Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,618,494 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,618,4940AT99.6% 611.8 / NA 262intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (135/125);  total (135/127)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,618,4950GC99.6% 616.1 / NA 262intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (134/127);  minor base A (1/0);  total (135/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,618,4960CG99.6% 608.2 / NA 262intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (133/127);  total (135/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCAGCCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTT  >  CP042583/1618214‑1618774
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
ttACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGaa                                                                                                                                                                                                                                                                                   >  2:1533004/1‑289 (MQ=255)
                aCGATCGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAACCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATAACA‑AAATtcgc                                                                                                                                                                                                                                                                   >  2:1681560/1‑288 (MQ=255)
                     cGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGAt                                                                                                                                                                                                                                                             <  1:1960126/289‑1 (MQ=255)
                      gCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATa                                                                                                                                                                                                                                                            <  1:695363/289‑1 (MQ=255)
                                   gtcactgtcCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGAt                                                                                                                                                                                                                                               <  1:1379434/289‑1 (MQ=255)
                                   gtcactgtcCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGAt                                                                                                                                                                                                                                               <  2:666169/289‑1 (MQ=255)
                                    tcactgtcCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGa                                                                                                                                                                                                                                                              >  2:315875/1‑273 (MQ=255)
                                    tcactgtcCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGa                                                                                                                                                                                                                                                              <  1:315875/273‑1 (MQ=255)
                                              aaTCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCCAGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGGTGTACGTATTAACTTTTCTCACGGATCGCCTGAAGATCACA‑GAATTGGTCCGGAATAAGTACGTGAGCTTGACGccacac                                                                                                                                                                                                                                    >  2:907816/1‑284 (MQ=255)
                                              aaTCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATgcg                                                                                                                                                                                                                                                                    <  2:1756177/257‑1 (MQ=255)
                                               aTCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCg                                                                                                                                                                                                                                          >  2:1221960/1‑282 (MQ=255)
                                               aTCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCg                                                                                                                                                                                                                                          <  1:1221960/282‑1 (MQ=255)
                                               aTCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCaaa                                                                                                                                                                                                                                      >  1:101170/1‑286 (MQ=255)
                                                   tataCGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTgggg                                                                                                                                                                                                                               <  1:1370540/289‑1 (MQ=255)
                                                   tataCGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTgggg                                                                                                                                                                                                                               <  1:1175499/289‑1 (MQ=255)
                                                    ataCGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCg                                                                                                                                                                                                                                          <  2:1419786/277‑1 (MQ=255)
                                                    ataCGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGc                                                                                                                                                                                                                              >  1:1060455/1‑289 (MQ=255)
                                                        tacgtCCGATTGAGATGAATTGATCGCAATCGCGGTGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTAAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAAAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCa                                                                                                                                                                                                                          <  2:1322306/285‑1 (MQ=255)
                                                         aCATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCGACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCAt                                                                                                                                                                                                                         <  2:777885/289‑1 (MQ=255)
                                                          cATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCAtg                                                                                                                                                                                                                        >  1:1941841/1‑289 (MQ=255)
                                                          cATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCAtg                                                                                                                                                                                                                        >  2:1071155/1‑289 (MQ=255)
                                                           aTCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCAtgt                                                                                                                                                                                                                       >  1:1333313/1‑289 (MQ=255)
                                                            tCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCAtgtg                                                                                                                                                                                                                      >  1:261073/1‑289 (MQ=255)
                                                             ccGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGATTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTgg                                                                                                                                                                                                                     <  1:1246691/289‑1 (MQ=255)
                                                                    agaTTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAACGTTCGTGAGATTGCCGCAAAACTGGGGCGTCCTGTGGCTATTCt                                                                                                                                                                                                              >  2:2181176/1‑289 (MQ=255)
                                                                      aTTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTgg                                                                                                                                                                                                            >  2:884278/1‑289 (MQ=255)
                                                                      aTTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTgg                                                                                                                                                                                                            >  1:688329/1‑289 (MQ=255)
                                                                       ttAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTggg                                                                                                                                                                                                           <  1:2161520/289‑1 (MQ=255)
                                                                       ttAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTggg                                                                                                                                                                                                           <  2:261073/289‑1 (MQ=255)
                                                                           tttATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGAc                                                                                                                                                                                                       <  2:727465/289‑1 (MQ=255)
                                                                               tCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTc                                                                                                                                                                                                    >  1:1647508/1‑288 (MQ=255)
                                                                                cGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATa                                                                                                                                                                                                                                                            <  2:1945189/231‑1 (MQ=255)
                                                                                   cATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAgg                                                                                                                                                                                                <  1:2225328/288‑1 (MQ=255)
                                                                                      cgcgGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGcc                                                                                                                                                                                            <  1:285085/289‑1 (MQ=255)
                                                                                      cgcgGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGc                                                                                                                                                                                             >  1:50535/1‑288 (MQ=255)
                                                                                             ttATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATc                                                                                                                                                                                     <  1:801425/289‑1 (MQ=255)
                                                                                              tATTTCCTTCGGATGTCATGTTCAAGCAACACCTTGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGAAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATcc                                                                                                                                                                                    <  1:1207984/289‑1 (MQ=255)
                                                                                              tATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATcc                                                                                                                                                                                    <  2:1064516/289‑1 (MQ=255)
                                                                                                     ttCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATc                                                                                                                                                                             <  1:384355/289‑1 (MQ=255)
                                                                                                     ttCGGATTTCATGTTAAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTGAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATc                                                                                                                                                                             <  1:977434/289‑1 (MQ=255)
                                                                                                          aTTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCTCGGATAAAGTTCGTGAGTTTGCCGCAAAACCGGGGCGTCATGCGGCTATTCTGGGTCACCTCCCGGGGTCCAAAATCCGTGTATCCAc                                                                                                                                                                          >  2:1699467/1‑287 (MQ=255)
                                                                                                          aTTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCt                                                                                                                                                                        <  2:1048577/289‑1 (MQ=255)
                                                                                                          aTTTCAGGTCTAAGCAACACCTGATTCGTCCACACAACGGAGAATCACATGTCAAAAAGGCTTCGCAGACCAAAAATCGTTCCCACGTTAGGACCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGTGGGTGCCAACGTTGTACGTATGAACTTTTCCCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTGCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCt                                                                                                                                                                        <  2:554069/289‑1 (MQ=255)
                                                                                                                tGTTCCAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAaag                                                                                                                                                                  <  1:1412499/289‑1 (MQ=255)
                                                                                                                   tCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAaagaag                                                                                                                                                               >  1:78468/1‑289 (MQ=255)
                                                                                                                    cAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGCACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAaagaag                                                                                                                                                               >  1:1754030/1‑288 (MQ=255)
                                                                                                                      aGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCTACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTg                                                                                                                                                                                                         >  1:1399751/1‑244 (MQ=255)
                                                                                                                           ccccTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGtt                                                                                                                                                       <  1:1546834/287‑1 (MQ=255)
                                                                                                                                ggTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCt                                                                                                                                                  <  1:1618143/289‑1 (MQ=255)
                                                                                                                                 ggtgttTCAGTCAACGGAGTATTAAATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCAAGAAACAGATCGAGCTAATCATCTTGAAAAATTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAATTTCGGGAGATTGCCGCCAAACTGGGGCCTCATGTGGCTACTCTGGGTGACATCCCGGGTCACAAAATCCGGTCAACCACCTTTAAATAAGGACAATTTTTccac                                                                                                                                                 >  2:1641954/3‑287 (MQ=255)
                                                                                                                                      ttCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATc                                                                                                                                                                             <  1:855475/256‑1 (MQ=255)
                                                                                                                                       tCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCCCA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGCGGCTATTCTGGGTGACCTCCAGTGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAACGTGTTCCTCAATAtt                                                                                                                                           >  2:1895867/1‑289 (MQ=255)
                                                                                                                                               cGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTAGGGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCTTGTTACCACCTTTAAAGTAGGCACAGTTTTCCTCAACATTGGGGATaa                                                                                                                                   >  2:1430002/1‑289 (MQ=255)
                                                                                                                                                            gTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTTTATCCACCTTTAAAGAAGGCAAAGGTTTCCTCAATATTGGGGATAAATTCCTGCTCGAc                                                                                                                      >  1:2201157/1‑289 (MQ=255)
                                                                                                                                                              ccAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGa                                                                                                                                                                                                        >  1:1695953/1‑205 (MQ=255)
                                                                                                                                                              ccAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTaaa                                                                                                                                                                   >  2:1686148/1‑242 (MQ=255)
                                                                                                                                                              ccAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACg                                                                                                                     <  2:1785059/288‑1 (MQ=255)
                                                                                                                                                               cAGAAGGCTTCGGAGAACAAAAATAGTTCCCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATGGCGGCGGGTGCCAACGTGGTACGTATGAACTTTTATAACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGcc                                                                                                                   <  2:1155432/289‑1 (MQ=255)
                                                                                                                                                                aGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCa                                                                                                                  >  1:2146049/1‑289 (MQ=255)
                                                                                                                                                                 gAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCTCGCGGATAAAGTTCGTGTGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAAGATTGGGGATAAATTCCTGCCCGACTCCa                                                                                                                  >  2:528599/1‑288 (MQ=255)
                                                                                                                                                                  aaGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCaaaa                                                                                                                                                                                       >  2:2203933/1‑218 (MQ=255)
                                                                                                                                                                   aGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAAc                                                                                                                <  2:361874/288‑1 (MQ=255)
                                                                                                                                                                       ttCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTggg                                                                                                           >  1:1278644/1‑289 (MQ=255)
                                                                                                                                                                        tCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATACTCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTGTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGt                                                                                                          <  1:2014869/289‑1 (MQ=255)
                                                                                                                                                                         cGCAGAACAAAGAGCGTTACCTCGTTAGGCCCAGCAACAGATCGCGATAATAATATTGAAAAAGTTATCGCGGCGGATGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTa                                                                                                         <  2:472754/289‑1 (MQ=255)
                                                                                                                                                                         cGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCa                                                                                                                                                                                                                          >  2:1396610/1‑176 (MQ=255)
                                                                                                                                                                         cGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTa                                                                                                         <  1:1038470/289‑1 (MQ=255)
                                                                                                                                                                                      tCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGcc                                                                                                                   <  1:909849/266‑1 (MQ=255)
                                                                                                                                                                                      tCGTGACCCCGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGa                                                                                            <  1:1827911/289‑1 (MQ=255)
                                                                                                                                                                                               cGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGaaaa                                                                                   <  1:1126549/289‑1 (MQ=255)
                                                                                                                                                                                               cGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGGTACCT‑CAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGaaaaa                                                                                  >  2:1241429/1‑289 (MQ=255)
                                                                                                                                                                                                       ccAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTa                                                                           <  1:1870867/289‑1 (MQ=255)
                                                                                                                                                                                                         aGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAAc                                                                                                                >  1:1091929/1‑250 (MQ=255)
                                                                                                                                                                                                          gCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCCAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCGCAATATTGGGGATAAATTCCTGCCCGACGCCAACCTGGTAAAAGGTAAAGGCAACAAAGAAAAAGTCGGTATCg                                                                        >  2:1191338/1‑289 (MQ=255)
                                                                                                                                                                                                          gCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCa                                                                                                                                                                                                                          >  1:2031498/1‑143 (MQ=255)
                                                                                                                                                                                                          gCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCa                                                                                                                                                                                                                          <  2:2031498/143‑1 (MQ=255)
                                                                                                                                                                                                          gCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCCGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAATGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGCAAAGGTGAAGGCGACAAAGCAAACGTCggcaac                                                                         >  2:1246562/1‑284 (MQ=255)
                                                                                                                                                                                                          gCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCg                                                                                                                        >  2:1617776/1‑241 (MQ=255)
                                                                                                                                                                                                                   cgcgATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTg                                                                                                                                                                                                         >  1:762428/1‑151 (MQ=255)
                                                                                                                                                                                                                     cgATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCt                                                                                                                          <  2:1307611/228‑1 (MQ=255)
                                                                                                                                                                                                                     cgATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCt                                                                                                                          >  1:1307611/1‑228 (MQ=255)
                                                                                                                                                                                                                        taatagTCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGACCCTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGGTCGTGAGATTGCCGCAGAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAGGAAGGCAAAGTTTTCCTCAATATTGGGAATAAATGCCTGCTCGACGCCAACCGGGGTAAAGGTGAAGGCGACAAAGAACAAGTCGGTATCGACTACAAAGgcctg                                                          <  2:439747/289‑1 (MQ=255)
                                                                                                                                                                                                                        taataaTCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTa                                                                                                         <  1:622202/242‑1 (MQ=255)
                                                                                                                                                                                                                        taataaTCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTa                                                                                                         >  2:622202/1‑242 (MQ=255)
                                                                                                                                                                                                                         aatagtCTTGAAAAAGTTATCGCGGCGGGTGACAACGTTTTACGTATGAACTTTTCTCACGGCTCGCCGGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGgcctgc                                                         <  2:2102799/289‑1 (MQ=255)
                                                                                                                                                                                                                         aataatCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGgcctgc                                                         <  1:1464594/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaGTTATCGCGGGGGGTGCGAACGTTGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGAAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGAcgtcg                                             <  1:533823/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     gTGCCAACGTTTTACGTATGAACTTTTCTCACGGCACGCCTGAAGATCACA‑CAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGTCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATcc                              <  1:1434022/288‑1 (MQ=255)
                                                                                                                                                                                                                                                              ttGTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAGGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTgg                     >  1:177695/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                gTACGTATGAACTTTTCTCGCGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGAcg                                                <  2:1110898/259‑1 (MQ=255)
                                                                                                                                                                                                                                                                gTACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACg                  >  1:395789/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 tACGTATGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGCCGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATACTGCTGCTGGACGa                 >  1:14768/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      aTGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAAc                                                                                                                >  1:209864/1‑189 (MQ=255)
                                                                                                                                                                                                                                                                      aTGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGt             <  1:22400/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTc            >  1:1005573/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      aTGAACTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTc            >  2:919447/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         aaCTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGaa                                                                                     <  2:1980556/213‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aaCTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGaa                                                                                     >  1:1980556/1‑213 (MQ=255)
                                                                                                                                                                                                                                                                          aCTTTTCTCACGGCTCGCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGt        <  2:101170/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cTTTTCTCACAGCTCGCCTGAAGAT‑ACAGAAATGCGTGCGGATAAAGTTCGTGAGATTGCCGGAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGTTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTc       <  2:2064819/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
TTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACCACGTTAGGCCCAGCAACAGATCGCGATAATAATCTTGAAAAAGTTATCGCGGCGGGTGCCAACGTTGTACGTATGAACTTTTCTCACGGCAGCCCTGAAGATCACA‑AAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTT  >  CP042583/1618214‑1618774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A