Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,677,451 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,677,4510AT99.5% 437.4 / NA 183intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (91/91);  minor base G (0/1);  total (91/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,677,4520GC100.0% 433.5 / NA 183intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (91/92);  total (91/92)
*CP0425831,677,4530CG100.0% 437.7 / NA 184intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (91/93);  total (91/93)

Only 100 of 184 total aligned reads displayed.

CGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGAGCATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATA  >  CP042583/1677171‑1677734
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                         
            aaaaGCGTTGGATGTAAGAAAGCCTGCCGCCTGGCAAAATACCCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGAt                                                                                                                                                                                                                                                                          <  2:278693/288‑1 (MQ=255)
            aaaaGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGAt                                                                                                                                                                                                                                                                          <  1:1696636/288‑1 (MQ=255)
            aaaaCCGCGGGAGTTAGGCAGGCCTGCCGCGGGGCAAAATAACCTGCAACGGACGCCAGCAGGTAAAGTTTCCGGTTATAGTAGGT‑TCTAGGAATCGGGGGTGGATAAAGCCGATACCGCCGCTAATGCTGGTGGCCTGAAAGCCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAGACATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGAt                                                                                                                                                                                                                                                                          <  2:1223756/288‑1 (MQ=255)
                 cgcTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGttt                                                                                                                                                                                                                                                                    >  1:147921/1‑289 (MQ=255)
                           cAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGGATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATGGGTTTTCCGCTTcac                                                                                                                                                                                                                                                          >  2:1154678/1‑289 (MQ=255)
                                     gccgccTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCCCTGCACAAATTGCTg                                                                                                                                                                                                                                                 >  2:1602586/1‑288 (MQ=255)
                                               aaaaTACACTGCAACTGACGCCAGCAGATAAAGTTTCCGGTTCTCATACCT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCg                                                                                                                                                                                                                                       <  1:1901303/288‑1 (MQ=255)
                                               aaaaTAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCg                                                                                                                                                                                                                                       <  1:403660/288‑1 (MQ=255)
                                                aaaTAACCTGCCACTGACGCCAGCAGATAAAGTTGCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGaa                                                                                                                                                                                                                                     <  2:1384072/289‑1 (MQ=255)
                                                        tGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAg                                                                                                                                                                                                                             <  1:123164/289‑1 (MQ=255)
                                                          ccACTGCAGCCAGCAGATAAAGTTTCCGGTTATAAAAACT‑TCTATGAATTCGGCCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCa                                                                                                                                                                                                                           <  2:2239698/289‑1 (MQ=255)
                                                              tGACGCCGGTAGATAAACTTTCCGTTTGTGATTACTCTCT‑TGAATTCGGGCTGGAACATGCCGTTCCCGCCGCTAATGCTGGTAGCCTGAAAACAGATCCATGGACACTGAAAATAAGCGGCGAAGTGGCAAAACCATTGAACCTCGATCATGATGACTTAACGCGTCGCTTCCAGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTATTCCGCTGCACAAATTGCTGGCGCTTGCAGAAACTACCAGCAATGc                                                                                                                                                                                                                       <  2:643473/289‑1 (MQ=255)
                                                               gACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCg                                                                                                                                                                                                                      <  2:543997/289‑1 (MQ=255)
                                                               gACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTAGAAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCg                                                                                                                                                                                                                      <  1:128/289‑1 (MQ=255)
                                                                aggcaagcagGTAAAGTTTCCGGTTATAATGATT‑GTGATGAGTTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCg                                                                                                                                                                                                                      <  2:2003238/283‑1 (MQ=255)
                                                                    cagcagATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAATCCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTa                                                                                                                                                                                                                 >  1:954061/1‑289 (MQ=255)
                                                                     agcagATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTAt                                                                                                                                                                                                                >  1:707869/1‑289 (MQ=255)
                                                                     agcagATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTAt                                                                                                                                                                                                                >  2:470619/1‑289 (MQ=255)
                                                                                tttCCGTTAATAATACTT‑TCTATGAATTCGGGCTGAATAAAGCCGCTCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAg                                                                                                                                                                                                                             <  2:230648/265‑1 (MQ=255)
                                                                                  tCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGaaa                                                                                                                                                                                                   >  2:1214274/1‑289 (MQ=255)
                                                                                  tCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGaa                                                                                                                                                                                                    >  2:752251/1‑288 (MQ=255)
                                                                                        tataATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCCACAATTCGAAGTATGTCACTTTCCAAACAAttt                                                                                                                                                                                             >  2:446736/1‑289 (MQ=255)
                                                                                         taaataaCT‑GCTACAAGTTCTGGCTGGATAAATCCGATGCCGCCGCTCATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTa                                                                                                                                                                                            <  2:400332/287‑1 (MQ=255)
                                                                                         ataataTCT‑TCGAGAGATTCGGGTGCGATAAAGCCATGCCCGCCGCTAATGCTGGTAGCCTGAACACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTa                                                                                                                                                                                            <  2:413269/289‑1 (MQ=255)
                                                                                                 ctgttaggAATTCGGGTTGGATAAGGCCAATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCg                                                                                                                                                                                     <  2:1260517/282‑1 (MQ=255)
                                                                                                            tCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATgcc                                                                                                                                                                          >  1:2151880/1‑289 (MQ=255)
                                                                                                              gggCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATgccag                                                                                                                                                                        >  1:840679/1‑289 (MQ=255)
                                                                                                               ggCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATgccag                                                                                                                                                                        >  1:166885/1‑288 (MQ=255)
                                                                                                                    gATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATgccaggccag                                                                                                                                                                   <  2:166885/288‑1 (MQ=255)
                                                                                                                         accgaaccccGCCGCAAAAGCTGGTAGCCAGTAAACCGATCCAAGGACACTGAAAAGCAAGAGCCGAGTGGCAAAACCATTGACCCTAGATTAGGGTGACGTAACCCGTCGCTTACCGCTGGAAGAGCGTATTTATCGTATGCGCTGGGTGGAAGCGAGGTCGATGGTGGTGCCGTGGAGTGGTTGTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGaa                                                                                                                                                             <  2:1584783/282‑2 (MQ=255)
                                                                                                                            cGATCCCGACGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGc                                                                                                                                                          <  2:834348/289‑1 (MQ=255)
                                                                                                                              aTCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCtt                                                                                                                                                        >  1:2085634/1‑289 (MQ=255)
                                                                                                                                 ccgccgCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTAt                                                                                                                                                     <  2:594532/289‑1 (MQ=255)
                                                                                                                                 ccgccgCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTAt                                                                                                                                                     <  1:1901506/289‑1 (MQ=255)
                                                                                                                                    ccgcTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATcgg                                                                                                                                                  >  2:2223083/1‑289 (MQ=255)
                                                                                                                                      gcTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATcggcg                                                                                                                                                <  1:319679/289‑1 (MQ=255)
                                                                                                                                           tGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTggg                                                                                                                                           <  1:653319/289‑1 (MQ=255)
                                                                                                                                              tGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGaa                                                                                                                                                                                  <  1:533199/247‑1 (MQ=255)
                                                                                                                                               ggTAGCCTGAAAACCGATCCATGGCCACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGCCTTAACCCGTCGCTTCCCGCTGGAAGAGCGAATTTATCGTATGCGCTGCGTGGAACCGTGGTCGATGGTCGTGCCGTGGACTGCTTTTCCGCTGCACAAATGGCTGGCGCTTGCCCAACCGACCAGCAATGCGAAGTATGTCCCTTTCGACACATTTGACGCACCGGAACAGATGCCAGGCCCGCAGGCCCGCCTTATCGGCGGGGGGCGGa                                                                                                                                       >  2:1566371/1‑289 (MQ=255)
                                                                                                                                                    ccTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACACGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAata                                                                                                                                   >  1:1909746/1‑288 (MQ=255)
                                                                                                                                                      tGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATcc                                                                                                                                <  1:466259/289‑1 (MQ=255)
                                                                                                                                                       gAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCt                                                                                                                               <  1:1037982/289‑1 (MQ=255)
                                                                                                                                                         aaaCCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACACGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTa                                                                                                                             <  2:1909746/289‑1 (MQ=255)
                                                                                                                                                               aTCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGa                                                                                                                       >  1:2106326/1‑289 (MQ=255)
                                                                                                                                                               aTCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGa                                                                                                                       >  1:19265/1‑289 (MQ=255)
                                                                                                                                                                   aTGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGa                                                                                                                   >  2:1798479/1‑289 (MQ=255)
                                                                                                                                                                         acTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGt                                                                                                             <  1:1175548/289‑1 (MQ=255)
                                                                                                                                                                           tGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGtc                                                                                                            >  1:1572891/1‑288 (MQ=255)
                                                                                                                                                                            gAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGTTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGtctc                                                                                                          >  1:2131102/1‑289 (MQ=255)
                                                                                                                                                                                   aGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAg                                                                                                   >  2:2012084/1‑289 (MQ=255)
                                                                                                                                                                                     cggcgAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATcggcg                                                                                                                                                <  1:1320739/242‑1 (MQ=255)
                                                                                                                                                                                     cggcgAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATcggcg                                                                                                                                                >  2:1320739/1‑242 (MQ=255)
                                                                                                                                                                                     cggcgAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCa                                                                                                 >  2:1737801/1‑289 (MQ=255)
                                                                                                                                                                                      ggcgAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGATCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAg                                                                                                   <  1:2223083/286‑1 (MQ=255)
                                                                                                                                                                                           aGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                            <  1:197152/288‑1 (MQ=255)
                                                                                                                                                                                           aGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                            <  2:2106326/288‑1 (MQ=255)
                                                                                                                                                                                           aGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                            <  1:1214274/288‑1 (MQ=255)
                                                                                                                                                                                                 aaaaCCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTc                                                                                     <  2:734643/289‑1 (MQ=255)
                                                                                                                                                                                                    aCCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTcaca                                                                                  <  1:1798479/289‑1 (MQ=255)
                                                                                                                                                                                                       aTTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGACGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTAGCGGCGGTGGGCTGACATATCCTTATGTCTAAGGATTCAGTCCCGACGACGCACTGCATCCGCCCACACtg                                                                               >  2:1095795/1‑289 (MQ=255)
                                                                                                                                                                                                       aTTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtg                                                                               >  1:1600406/1‑289 (MQ=255)
                                                                                                                                                                                                       aTTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtg                                                                               >  1:240834/1‑289 (MQ=255)
                                                                                                                                                                                                       aTTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGACCGGCCAGCAGGACCGCTTTATCGGCGGTGGGCGGAAATATCCTTCTGTCGAAGGATTGCGTCTCTCGGAAGCAATGCATCCGCTcccc                                                                                  >  2:617944/1‑283 (MQ=255)
                                                                                                                                                                                                        ttGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                            <  2:66869/275‑1 (MQ=255)
                                                                                                                                                                                                         tGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtg                                                                               >  1:1039472/1‑287 (MQ=255)
                                                                                                                                                                                                         tGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtg                                                                               <  2:1039472/287‑1 (MQ=255)
                                                                                                                                                                                                         tGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGAATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtgat                                                                             <  1:166387/289‑1 (MQ=255)
                                                                                                                                                                                                               tCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAg                                                                                                   <  1:1077254/261‑1 (MQ=255)
                                                                                                                                                                                                               tCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGt                                                                       >  1:1126669/1‑289 (MQ=255)
                                                                                                                                                                                                                 gATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGCGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCGTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAg                                                                     <  1:1761997/289‑1 (MQ=255)
                                                                                                                                                                                                                      tgatgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGtt                                                                >  1:1985693/1‑289 (MQ=255)
                                                                                                                                                                                                                       gatgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcga                                                                                                        >  1:1296627/1‑248 (MQ=255)
                                                                                                                                                                                                                       gatgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcga                                                                                                        <  2:1296627/248‑1 (MQ=255)
                                                                                                                                                                                                                        atgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAAt                                                                                                                                                                                                                         <  1:993884/134‑1 (MQ=255)
                                                                                                                                                                                                                             ttAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTa                                                                                                                                                                                            >  1:289322/1‑158 (MQ=255)
                                                                                                                                                                                                                             ttAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTa                                                                                                                                                                                            <  2:289322/158‑1 (MQ=255)
                                                                                                                                                                                                                             ttAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCa                                                         >  2:515658/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACAGTAGGGGTTTATGGCAAGGCGtt                                                  >  1:1548940/1‑289 (MQ=255)
                                                                                                                                                                                                                                     tCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTggg                                                                                                                                           <  1:1716582/199‑1 (MQ=255)
                                                                                                                                                                                                                                     tCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGACGGATTGCGTCTCGACGAAGCAATGGATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGtt                                                  >  1:333153/1‑288 (MQ=255)
                                                                                                                                                                                                                                     tCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGc                                                                                       <  1:8261/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        cGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGgcgcg                               >  1:453389/1‑288 (MQ=255)
                                                                                                                                                                                                                                                          tATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATcggcg                                                                                                                                                >  1:225409/1‑173 (MQ=255)
                                                                                                                                                                                                                                                               aTCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcgacga                                                                                                     >  2:882620/1‑211 (MQ=255)
                                                                                                                                                                                                                                                                tCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGa                      >  2:347174/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 cGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCg                       >  1:1878967/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                    aTGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGCCGAAGGATTGCGTCCCGACGACGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAACTGGCGACCCGGCGCGACt                    >  2:495077/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                    aTGCGCTGCGTGGAAGCGTGATCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCATTCGAAACAATTTATGCACCGGAACAGATGCCAGGCTAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTGATGTCGAAGTATTGCGTCTCGACGCAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGACGGTGCGACTGa                  <  1:241133/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                            >  2:1204958/1‑214 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                            <  1:1204958/214‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtgat                                                                             <  2:1664765/229‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGc                                                          >  1:1349341/1‑248 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGc                                                          <  2:1349341/248‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGAt                 >  1:1461325/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCCGCAATATCCTTATGTCGAAGGATGTCGTCCCGACGAAGCAATGCTCCCGCTCACCATGCGGCCCGACGTCGTTTATGCCTAGGCCTTACCCCCACCAAAAGGCCCCCCCGTGccgccgct                 >  2:2149987/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTACACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCCCGCCCGTGCGCCTgct                 >  2:1397202/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                       cgcTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCt                                                                                                                               <  2:1638800/177‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATg                                                                                                                                                                                          <  1:1836757/113‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATg                                                                                                                                                                                          >  2:1836757/1‑113 (MQ=255)
                                                                                                                                                                                                                                                                                     gtggtgGATGGTGGTGCCGGGGATTAGTTTACGGCGGCACAAATTGATGGAGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGAACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAata  <  2:1758776/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                         
CGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT‑TCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATGCTGGTAGCCTGAAAACCGATCCATGGACACTGAAAATCAGCGGCGAAGTGGCAAAACCATTGACCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGAGCATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATA  >  CP042583/1677171‑1677734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A