Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F210 I1 R1	4928	385.2	6658600	97.5%	6492135	231.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	1,517,132	3 bp→TCG	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	1,517,132	0	A	T	99.7%	836.1 / NA	353	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (184/167); minor base C (0/1); total (184/169)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*	CP042583	1,517,133	0	G	C	99.7%	830.6 / NA	353	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (183/168); total (184/169)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,517,134	0	C	G	99.7%	836.5 / NA	353	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (183/168); total (184/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 353 total aligned reads displayed.

TACTACCGTATAACCCTCATCATCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCAGCTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GAGCCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGAGCTGGACCAGTAGCATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTAGTATACGTTTTCGCAGCTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTATTGTTTATTCCTGGGCG  >  CP042583/1516859‑1517413

|||

gactacCGTATAACCCTCCTCAGCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTCCGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGttcg                                                                                                                                                                                                                                                                              <  2:2440189/287‑4 (MQ=255)

         ataACCCTCATCATCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggc                                                                                                                                                                                                                                                                     >  1:143202/1‑288 (MQ=255)

          taACCCTCATCATCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgc                                                                                                                                                                                                                                                                   >  1:2599546/1‑289 (MQ=255)

              ccTCATCATCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggcg                                                                                                                                                                                                                                                               <  1:558583/289‑1 (MQ=255)

               cTCATCATCCTGTTGTTCGCTTTGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTTGGGCGGCTTGCTTTCGTGGACCATTTCGATGGCCggcgcggcgc                                                                                                                                                                                                                                                              >  2:3212696/1‑289 (MQ=255)

               cTCATCATCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                                                                                                                                                                                                              >  1:1764239/1‑289 (MQ=255)

                  atcatcCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtgt                                                                                                                                                                                                                                                           >  2:1712457/1‑289 (MQ=255)

                  atcatcCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTCGACCAACAGGGTATAGAACGCTTTATTTAGTCTTACGGCGCACTGGCGGCAGTT‑GTCGCGTTCTTGTTTCTGATTTTGCAGGCCATTGCTGCTCTGCTACCTGCGTTTTTGATTAGCTTTGCCAATGCTGTGCC‑GTTTGGCGCGTCCTGGGGCGGCTTGCTGTCGTGGACCAGTTCCAGGTCCGGCGCGCGGCtgg                                                                                                                                                                                                                                                           >  2:2596659/1‑288 (MQ=255)

                       ccTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTCTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCCCGGCGCTGTGCttt                                                                                                                                                                                                                                                      >  1:1475176/1‑289 (MQ=255)

                            tgttCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAt                                                                                                                                                                                                                                                 <  1:3187/289‑1 (MQ=255)

                                      gctgctGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                                                                                                                                                                                                                       <  2:840430/289‑1 (MQ=255)

                                        tgttgGGATGGGCGCTGCTTCCCGGAGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCCCTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGAt                                                                                                                                                                                                                                     <  2:1466007/286‑1 (MQ=255)

                                                tGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCcgcg                                                                                                                                                                                                                             <  1:1712457/289‑1 (MQ=255)

                                                 gggCGCTGCTTCCGGTCGGCCATGCGTTGATCAAGCGCAGGGTTGGGGCGTTTGCGGCCGTTGACCAACAGGGTATAGAACGCTGTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GGCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTGCTGGGGCGGCTTGCTGTCGTGGACCAGTGCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCcgcg                                                                                                                                                                                                                             <  2:2031929/288‑1 (MQ=255)

                                                       tgctTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAggggt                                                                                                                                                                                                                      >  1:2301714/1‑289 (MQ=255)

                                                                           tttATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCt                                                                                                                                                                                                                                                        >  1:2003359/1‑235 (MQ=255)

                                                                                   tCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCaaaa                                                                                                                                                                                                  <  2:1581124/281‑1 (MQ=255)

                                                                                    cGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                                                                                                                                                                                                                       <  2:2973063/243‑1 (MQ=255)

                                                                                    cGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCCGTGCTTTTTTATCGCCCGAGTGATGGGCCGCGAAGGGGTGGAAAAATTAACCGGCAAAACCATgtctt                                                                                                                                                                                         >  2:3135273/1‑285 (MQ=255)

                                                                                             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATgg                                                                                                                                                                                <  2:196454/289‑1 (MQ=255)

                                                                                                           cgtcacccAACAGGGTATCGATCTCTTTATTCAGTCGTCCGGCGCATTGGCGGCAGTT‑GTCTCGTTCTTTTTGATGATTTTGCCGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACt                                                                                                                                                                  <  2:1530667/283‑1 (MQ=255)

                                                                                                                aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGTCGCACTGGCGGCTGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑Ct                                                                                                                                                             <  1:430495/288‑1 (MQ=255)

                                                                                                                    aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCtt                                                                                                                                                                                          >  1:2981591/1‑256 (MQ=255)

                                                                                                                          tataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGCGTGATGGGCCGCGAAGTGGTGGGAGACTTCACCGGCAAAACCGGTACTGACAGTATGGACGGCTTTTTCACTAC‑CCACGGCaacacc                                                                                                                                                  >  2:3047145/1‑285 (MQ=255)

                                                                                                                          tataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAacac                                                                                                                                                  >  2:1870747/1‑289 (MQ=255)

                                                                                                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAacaca                                                                                                                                                 <  1:2402098/289‑1 (MQ=255)

                                                                                                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAacaca                                                                                                                                                 <  1:1246739/289‑1 (MQ=255)

                                                                                                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAacaca                                                                                                                                                 <  1:2933171/289‑1 (MQ=255)

                                                                                                                            taGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGCTGGGCcgcg                                                                                                                                                                                                                             >  2:3306482/1‑213 (MQ=255)

                                                                                                                                     ttATTCAGTCTTCCGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGt                                                                                                                                       <  1:1976897/289‑1 (MQ=255)

                                                                                                                                      tATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGAc                                                                                                                                                                              >  1:3248405/1‑250 (MQ=255)

                                                                                                                                           aGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAt                                                                                                                                                                                                                                                 >  1:1636976/1‑178 (MQ=255)

                                                                                                                                           aGTCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAt                                                                                                                                                                                                                                                 <  2:1636976/178‑1 (MQ=255)

                                                                                                                                             tCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGc                                                                                                                                                        <  2:562313/264‑1 (MQ=255)

                                                                                                                                             tCTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGGTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGTCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCTGGCTTGACATGTTGGACGGCTTCTTCACTCGCCTAGGCAAAACACCCCAtgtggtgctgtcc                                                                                                                                 >  2:2373485/1‑276 (MQ=255)

                                                                                                                                              cTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCgg                                                                                                                                <  1:1870747/287‑1 (MQ=255)

                                                                                                                                              cTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATCGGCCGCGAAGTGGTGGAGAAATTACCCGGCAAAACCGTGCTTGACAGTATGGCCGGCTTTTCCACACG‑CTAACGCAACCACACCCTTTTGTTCTGTCggct                                                                                                                              >  2:3080964/1‑287 (MQ=255)

                                                                                                                                              cTTACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGATTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGGTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATAGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGtt                                                                                                                              >  1:584599/1‑289 (MQ=255)

                                                                                                                                                tACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAacaca                                                                                                                                                 <  1:1596338/268‑1 (MQ=255)

                                                                                                                                                tACGGCGCACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAGCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAACATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTGCACTCG‑CTACGGCAAACCCCCCATTCTGGTCTGTCGGTTAt                                                                                                                            >  2:1176706/1‑289 (MQ=255)

                                                                                                                                                     cgcACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCt                                                                                                                       >  1:1306502/1‑289 (MQ=255)

                                                                                                                                                      gcACTGTCGGCAGTTGGTCCG‑TTCTTGTTGCTGCGTTTGCGGGCCATTGCTGCACTGCGACCTGCGTTTTTGATCACCGTTTCCAATGC‑GCCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCtt                                                                                                                      <  2:2781494/289‑1 (MQ=255)

                                                                                                                                                       cACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCt                                                                                                                                                                                                                                                        <  1:1077792/159‑1 (MQ=255)

                                                                                                                                                       cACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGa                                                                                                                                                                               >  1:3286151/1‑232 (MQ=255)

                                                                                                                                                       cACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGa                                                                                                                                                                               <  2:3286151/232‑1 (MQ=255)

                                                                                                                                                       cACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTTG‑CTACGGCAAAAACCCCATTCTGGTCTGTCGGTTATTGCCttt                                                                                                                     >  2:1669376/1‑289 (MQ=255)

                                                                                                                                                       cACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCttt                                                                                                                     >  1:1819111/1‑289 (MQ=255)

                                                                                                                                                       cACTGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCttt                                                                                                                     >  1:946125/1‑289 (MQ=255)

                                                                                                                                                          tGGCGGCAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                                                                                                  <  1:2167376/289‑1 (MQ=255)

                                                                                                                                                            gcggcAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTa                                                                                                                                                                                                            >  1:2782903/1‑198 (MQ=255)

                                                                                                                                                                cAGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCtt                                                                                                             <  2:2322681/288‑1 (MQ=255)

                                                                                                                                                                 aGTT‑GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTc                                                                                                           >  1:3329113/1‑289 (MQ=255)

                                                                                                                                                                     tgtctcgttcttGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGa                                                                                                         >  1:886053/8‑288 (MQ=255)

                                                                                                                                                                                               aGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGa                                                                               <  1:699206/288‑1 (MQ=255)

                                                                                                                                                                                                      tgctgcACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTg                                                                                                                          <  2:800994/238‑1 (MQ=255)

                                                                                                                                                                                                           cACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGt                                                                                                                                                                                              <  1:2350065/166‑1 (MQ=255)

                                                                                                                                                                                                             ccGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGAc                                                                              <  2:2680615/275‑1 (MQ=255)

                                                                                                                                                                                                              cGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCg                                                               <  2:1306502/289‑1 (MQ=255)

                                                                                                                                                                                                                cTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCaaaa                                                                                                                                                                                                  >  1:3063540/1‑157 (MQ=255)

                                                                                                                                                                                                                cTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACt                                                                                                                                                                  <  2:1092277/189‑1 (MQ=255)

                                                                                                                                                                                                                cTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAacaca                                                                                                                                                 >  1:1554740/1‑205 (MQ=255)

                                                                                                                                                                                                                cTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGc                                                              >  1:2669077/1‑288 (MQ=255)

                                                                                                                                                                                                                cTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGc                                                              >  1:312322/1‑288 (MQ=255)

                                                                                                                                                                                                                cTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGGTATTGCCTTTGTTCCCTTTCGATCCAATCAGCTATGCTGCCGGGTTGACTCCAATACGTTTTccc                                                              >  2:2770925/1‑286 (MQ=255)

                                                                                                                                                                                                                cTACATGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGAt                                                                                                        <  1:1002079/246‑1 (MQ=255)

                                                                                                                                                                                                                  aCCTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAAt                                                                                                   <  1:2770968/249‑1 (MQ=255)

                                                                                                                                                                                                                   ccTGCGTTTTTGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCCTGCTGTCGTGGACCAGTTCGATGGCcggcgcggcg                                                                                                                                                                                                                                                               <  1:2362009/93‑1 (MQ=255)

                                                                                                                                                                                                                          ttttGATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTAt                                                   <  2:2834074/289‑1 (MQ=255)

                                                                                                                                                                                                                              gATCACCTTTGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCt                                                                                                                                                                                                                                                        >  1:2437170/1‑89 (MQ=255)

                                                                                                                                                                                                                                       tttcccatgcgtcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCt                                       <  1:3080964/275‑1 (MQ=255)

                                                                                                                                                                                                                                       tGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTAt                                                   <  2:1938274/276‑1 (MQ=255)

                                                                                                                                                                                                                                       tGCCAATGC‑GTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTAt                                                   >  1:1938274/1‑276 (MQ=255)

                                                                                                                                                                                                                                              tgcgtcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAg                                <  1:987263/282‑1 (MQ=255)

                                                                                                                                                                                                                                              tgcgtcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAg                                <  1:1141738/282‑1 (MQ=255)

                                                                                                                                                                                                                                               gcgtcgcTGTTTGGCGCGTTCTGGGGAGGCTTGCTGTCGTGGACCAGTTCGATGGCTGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCGGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGCGCTTGACAGTATGGAAGGATATTTAACTAG‑ATACGGCAAAAAAACCATTCTGGTCTGTCGGTTATTCCCTTTTGTCTCATTAGAATCAATCAGATATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCATCGGGCCTGGtgcat                               >  2:2004822/7‑285 (MQ=255)

                                                                                                                                                                                                                                                  tcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATTCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTa                             >  2:1060622/4‑288 (MQ=255)

                                                                                                                                                                                                                                                  tcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGt                                   >  1:2545383/4‑282 (MQ=255)

                                                                                                                                                                                                                                                  tcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTa                             >  2:636931/4‑288 (MQ=255)

                                                                                                                                                                                                                                                  tcgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTa                             >  1:2887170/4‑288 (MQ=255)

                                                                                                                                                                                                                                                   cgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgctgc                                                                                       >  1:1613084/3‑229 (MQ=255)

                                                                                                                                                                                                                                                   cgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgctgc                                                                                       <  2:1613084/227‑1 (MQ=255)

                                                                                                                                                                                                                                                   cgcTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTAcc                           <  2:946125/287‑1 (MQ=255)

                                                                                                                                                                                                                                                      tGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGc                        <  1:760828/289‑1 (MQ=255)

                                                                                                                                                                                                                                                      tGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGc                        <  1:636931/289‑1 (MQ=255)

                                                                                                                                                                                                                                                            gcgcGTTTGGGGGGGGCTGGCTTCCGTGGACCGGTTCGATGACCGGCGCGGCGCGGTGCTTTTGTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTa                   <  2:2887170/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                gTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTATTGt               <  1:2951588/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCaa                                                                                                                                                      <  2:7000/151‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAaca                                                                                                                                                   <  2:2361323/154‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCCCCCCCGTGCTTGGTCCGTTACCTGCAACTAtttgtta            >  2:2250778/1‑284 (MQ=255)

                                                                                                                                                                                                                                                                           gcTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGa                                                                                                                                                                                        <  2:2763560/109‑1 (MQ=255)

                                                                                                                                                                                                                                                                           gcTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGa                                                                                                                                                                                        >  1:2763560/1‑109 (MQ=255)

                                                                                                                                                                                                                                                                           gcTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCa                                                                                                     <  2:2526625/191‑1 (MQ=255)

                                                                                                                                                                                                                                                                           gcTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCa                                                                                                     >  1:2526625/1‑191 (MQ=255)

                                                                                                                                                                                                                                                                           gcTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTATTGTTTATTCCTgggc   >  2:2940476/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                           gcTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTATTGTTTATTCCTgggc   >  1:371172/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                            cTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCg                                                                                                                                                                <  2:445777/132‑1 (MQ=255)

                                                                                                                                                                                                                                                                            cTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGt                                                                                                                                       <  2:1010916/156‑1 (MQ=255)

                                                                                                                                                                                                                                                                            cTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGt                                                                                                                                       >  1:1010916/1‑156 (MQ=255)

                                                                                                                                                                                                                                                                            cTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGt                                   >  1:2707765/1‑256 (MQ=255)

                                                                                                                                                                                                                                                                            cTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACGTTTTCGCTCGTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTATTGTTTATTCCTgggcg  >  2:1399639/1‑289 (MQ=255)

|||

TACTACCGTATAACCCTCATCATCCTGTTGTTCGCTATGCTGCTGGCATGGGCGCTGCTTCCCGGCGTCCATGAGTTTATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTT‑GTCAGCTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGC‑GAGCCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGAGCTGGACCAGTAGCATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCG‑CTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTAGTATACGTTTTCGCAGCTTTTTTATCGCCACCGGGCTTGGTCAGTTACCGGCAACTATTGTTTATTCCTGGGCG  >  CP042583/1516859‑1517413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A