Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,668,530 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,668,5300GC99.7% 691.7 / NA 294intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (144/149);  minor base A (1/0);  total (145/149)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,668,5310CG99.0% 678.5 / NA 294intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base G (142/149);  total (145/149)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,668,5320TA98.6% 674.7 / ‑5.6 294intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (141/147);  minor base C (4/0);  total (145/149)
Fisher's exact test for biased strand distribution p-value = 5.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 294 total aligned reads displayed.

AGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCTTAGTTATCGACTACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACGCTGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCGCTAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGT  >  CP042583/1668261‑1668810
                                                                                                                                                                                                                                                                                |||                                                                                                                                                                                                                                                                                        
tcacgcagcgatattcatcaacgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGAt                                                                                                                                                                                                                                                                        <  1:2025943‑M2/88‑1 (MQ=255)
   cgcagcgatattcatcaacgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TCG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACTACCGAGTTACCGAA‑CTGGCGATAg                                                                                                                                                                                                                                                                      >  1:3187229‑M2/199‑288 (MQ=255)
   cgcagcgatattcatcaacgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatattgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAg                                                                                                                                                                                                                                                                      >  1:1357058‑M2/199‑288 (MQ=255)
      agcgatattcatcaacgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacccgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttggccctctttctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCACGCCGTTTTTCCCACGCGGCTAAG‑ACCTGCCCCACCACCCAGGTACCGAA‑CCGGCCAtagggc                                                                                                                                                                                                                                                                   >  2:553072‑M2/196‑286 (MQ=255)
        cgatattcatcaacgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCCAGTTACCGCA‑CTGGCGCTAGGCCTGa                                                                                                                                                                                                                                                                >  2:1752389‑M2/194‑289 (MQ=255)
                  caacgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAgtg‑tccgaa                                                                                                                                                                                                                                                      >  2:1906256‑M2/184‑284 (MQ=255)
                    acgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCCCCGCCTGT‑TTG‑ATTTTTGGCCCAAGCAGTTTTGCCACCGCGGCCAAT‑ACCGGCCCGACCACCGAGTTACCGAA‑CTGGCGATAGGCCtgagtg‑tccgaaacc                                                                                                                                                                                                                                                   >  2:3049907‑M2/182‑282 (MQ=255)
                     cgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCctgagtg‑tccgaaaccg                                                                                                                                                                                                                                                  >  1:1236446‑M2/181‑281 (MQ=255)
                     cgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCctgagtg‑tccgaaaccg                                                                                                                                                                                                                                                  >  1:1320605‑M2/181‑281 (MQ=255)
                     cgacaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGACACCGCCCGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCCAAG‑ACCGGCACGAACACCGCGTTACCGAA‑CTCGCGAaaggcctgagtg‑ttgtccccta                                                                                                                                                                                                                                                  >  2:615351‑M2/181‑280 (MQ=255)
                       acaaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCCCGCCCACCGAGTTACCGCA‑CTGGCCATAGgcccgtgtg‑tccgacacccga                                                                                                                                                                                                                                                >  2:1868196‑M2/179‑279 (MQ=255)
                        caaaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGa                                                                                                                                                                                                                                                <  1:3038988‑M2/111‑1 (MQ=255)
                                               ctgtcggccgtaacgcaaaatgccaggctctgccctgttaacagcctggcgatgcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCgg                                                                                                                                                                                                                         <  2:2531993‑M2/134‑1 (MQ=255)
                                               ctggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCgg                                                                                                                                                                                                                         <  1:2986836‑M2/134‑1 (MQ=255)
                                                tggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctaccgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgttaacgtcggccctgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGTCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCATGCCCCCCCAGTTACCGAACCTGCCGA‑CGGCCTTAGTGCTCG‑AAACCGGAAAACGGAATTTCCCTTTCCCcggg                                                                                                                                                                                                                        >  2:1727476‑M2/154‑287 (MQ=255)
                                                   cgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGCGTTACCGAA‑CTGGCCATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAAGTTCGCTTCCCCCCGCCCt                                                                                                                                                                                                                     >  2:2705613‑M2/151‑288 (MQ=255)
                                                      cctgaacgcgaaaggccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaa                                                                                                                                                                                                                 <  1:2529470‑M2/142‑1 (MQ=255)
                                                        tgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaaa                                                                                                                                                                                                                >  1:2172239‑M2/146‑288 (MQ=255)
                                                                          acctgcccggttaacagcgtggcgaggctcttctatatcgccgtctcacgcgtggcaatcgcgcgcatatttttgctgctggtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAgcgcgcg                                                                                                                                                                                                <  2:3263323‑M2/159‑1 (MQ=255)
                                                                             tgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTc                                                                                                                                                                                           >  1:1482109‑M2/125‑288 (MQ=255)
                                                                             tgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcatggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGa                                                                                                                                                                                                                                                                <  2:403154‑M2/96‑1 (MQ=255)
                                                                              gcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcCACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTCCAAAACCCATTAACCTCGCGCATTcc                                                                                                                                                                                          >  2:2477201‑M2/124‑288 (MQ=255)
                                                                              gcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCTAA‑CCGGCGATCGGCCCGATGG‑TCCGGAAACGGAATACGGAATTTCGCATCTCCCGGCGCTACAAAACCCATCAAGCGCGCGCATTcc                                                                                                                                                                                          >  2:3115302‑M2/124‑288 (MQ=255)
                                                                               gcaggttaaaagacgggcggggcgcttctttatcgccgtataacgcggggcaatcgcgagaagatttttgctgcgagtggccttatcgggaacgtcggccaggttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTccc                                                                                                                                                                                         <  2:3017627‑M2/166‑1 (MQ=255)
                                                                               cccggttaaaagccgggcgggggcgttctttagccccgtatcacgcgtggcaatcgcgggcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTccc                                                                                                                                                                                         <  2:3309773‑M2/166‑1 (MQ=255)
                                                                                ccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCTACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCg                                                                                                                                                                                        <  1:2260737‑M2/167‑1 (MQ=255)
                                                                                 cggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAg                                                                                                                                                                                      <  1:2705613‑M2/169‑1 (MQ=255)
                                                                                             ggggaggtcccttctcaacccccgtatcacgcgtggaaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgtcgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGAc                                                                                                                                                                          <  2:1683573‑M2/181‑1 (MQ=255)
                                                                                              ggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaa                                                                                                                                                                                                                 <  1:479274‑M2/142‑1 (MQ=255)
                                                                                                     cgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACg                                                                                                                                                                   <  1:1727476‑M2/188‑1 (MQ=255)
                                                                                                       cttctctgtggccgtatcccgcgtggcaatcggccgcatatttttgctgctagtggccttagcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGAt                                                                                                                                                                 <  2:1320605‑M2/190‑1 (MQ=255)
                                                                                                                  ccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGGAGATGTTTCTTATTCa                                                                                                                                                     >  1:2206407‑M2/88‑289 (MQ=255)
                                                                                                                     tatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCTCTAACCGACGTGGACGATGTTgctcattaggcg                                                                                                                                                  >  2:1606658‑M2/85‑284 (MQ=255)
                                                                                                                     tatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTg                                                                                                                                                                       <  2:2985102‑M2/184‑1 (MQ=255)
                                                                                                                     tatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCAATCCCGATGCCTTAACCGACCTGGACGAGGTTGCTGATTCcgcg                                                                                                                                                  >  2:965586‑M2/85‑289 (MQ=255)
                                                                                                                       tcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGAt                                                                                                                                                                                                                                                                        <  2:224477‑M2/88‑1 (MQ=255)
                                                                                                                       tcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAg                                                                                                                                                                                      >  1:1387443‑M2/83‑251 (MQ=255)
                                                                                                                       tcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAg                                                                                                                                                                                      <  2:1387443‑M2/169‑1 (MQ=255)
                                                                                                                       tcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGa                                                                                                                                                >  1:1935602‑M2/83‑289 (MQ=255)
                                                                                                                         acgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAg                                                                                                                                                                                                                                                                      >  2:2161596‑M2/81‑170 (MQ=255)
                                                                                                                         acgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAg                                                                                                                                                                                                                                                                      <  1:2161596‑M2/90‑1 (MQ=255)
                                                                                                                               ggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑tcc                                                                                                                                                                                                                                                         >  1:1220377‑M2/75‑175 (MQ=255)
                                                                                                                               ggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCg                                                                                                                                                                            <  1:147111‑M2/179‑1 (MQ=255)
                                                                                                                               ggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGGCaaa                                                                                                                                        >  1:792456‑M2/75‑289 (MQ=255)
                                                                                                                                     cgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCtt                                                                                                                                   >  1:2301011‑M2/69‑288 (MQ=255)
                                                                                                                                        gcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTtct                                                                                                                                <  1:3226090‑M2/223‑1 (MQ=255)
                                                                                                                                         cgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCg                                                                                                                                                                                        <  1:371543‑M2/167‑1 (MQ=255)
                                                                                                                                           catatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAc                                                                                                                             >  1:102178‑M2/63‑288 (MQ=255)
                                                                                                                                            atatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAccc                                                                                                                           <  1:1752389‑M2/228‑1 (MQ=255)
                                                                                                                                             tatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCg                                                                                                                          <  2:707263‑M2/229‑1 (MQ=255)
                                                                                                                                             tatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCg                                                                                                                          <  1:2896988‑M2/229‑1 (MQ=255)
                                                                                                                                             tatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCg                                                                                                                          <  1:2144649‑M2/229‑1 (MQ=255)
                                                                                                                                              gtttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGt                                                                                                                         <  1:814533‑M2/230‑1 (MQ=255)
                                                                                                                                                   tgctgggcgtggccgtgtcgtaaacgtcggccatttggtgcctcttgcggactcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCa                                                                                                                    <  2:2113763‑M2/235‑1 (MQ=255)
                                                                                                                                                      tgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCata                                                                                                                  >  1:1806017‑M2/52‑288 (MQ=255)
                                                                                                                                                          ggtggccttatcgtgaacttcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGCCTCAAGCAGTTTTGCCACCGCGGCAAAG‑AACGGCACGCCCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGCG‑TCCGAAACCGGAATACGGAATGTCACTTCTTCGGACGCTTCATAACCCATTACGCGCGCGCATTCACGAGGCGTGaa                                                                                                                                                                               >  2:2042622‑M2/48‑223 (MQ=255)
                                                                                                                                                          agtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATccc                                                                                                              >  1:2381834‑M2/48‑288 (MQ=255)
                                                                                                                                                                    tcgtgaacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaa                                                                                                                                                                                                                 >  1:2086986‑M2/38‑179 (MQ=255)
                                                                                                                                                                         aacgtcggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGAt                                                                                                                                                                 >  1:2331967‑M2/33‑222 (MQ=255)
                                                                                                                                                                              cggccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaaaa                                                                                                                                                                                                               >  1:2660577‑M2/28‑171 (MQ=255)
                                                                                                                                                                                gccatgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTc                                                                                        >  2:2319784‑M2/26‑288 (MQ=255)
                                                                                                                                                                                    tgttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCcg                                                                                      >  2:2184746‑M2/22‑286 (MQ=255)
                                                                                                                                                                                     gttgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGcc                                                                                  <  2:321928‑M2/269‑1 (MQ=255)
                                                                                                                                                                                       tgtggctctagctgcaggAACGCCAACG‑CTGTGTTG‑ATTTTTGGCTCATGCAGTTTTGCCACCGCGGCAAAG‑CCCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCAt                                                                                <  2:1227935‑M2/271‑1 (MQ=255)
                                                                                                                                                                                       tgtgcctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCTGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCAt                                                                                <  1:990916‑M2/271‑1 (MQ=255)
                                                                                                                                                                                           cctcttgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGa                                                                                                                                                                     <  1:1428491‑M2/186‑1 (MQ=255)
                                                                                                                                                                                                tgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCg                                                                                                                                                                                        <  2:934415‑M2/167‑1 (MQ=255)
                                                                                                                                                                                                tgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGa                                                                                                                                                >  1:670124‑M2/10‑216 (MQ=255)
                                                                                                                                                                                                tgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGcc                                                                                  >  2:2674552‑M2/10‑278 (MQ=255)
                                                                                                                                                                                                tgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                       <  2:1476849‑M2/280‑1 (MQ=255)
                                                                                                                                                                                                tgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                       >  1:3123315‑M2/10‑289 (MQ=255)
                                                                                                                                                                                                tgctgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                       <  1:908041‑M2/280‑1 (MQ=255)
                                                                                                                                                                                                   tgacgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaataa                                                                     <  2:1806017‑M2/282‑1 (MQ=255)
                                                                                                                                                                                                      cgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACg                                                                                                                                                                   <  2:1953434‑M2/188‑1 (MQ=255)
                                                                                                                                                                                                      cgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACg                                                                                                                                                                   >  1:1953434‑M2/4‑191 (MQ=255)
                                                                                                                                                                                                      cgcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTtct                                                                                                                                >  1:1122177‑M2/4‑226 (MQ=255)
                                                                                                                                                                                                       gcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAAcgcg                                                                 <  1:2572021‑M2/286‑1 (MQ=255)
                                                                                                                                                                                                       gcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACgcgc                                                                >  1:309015‑M2/3‑289 (MQ=255)
                                                                                                                                                                                                       gcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACgcgc                                                                <  1:1868196‑M2/287‑1 (MQ=255)
                                                                                                                                                                                                       gcAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGAACGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                       >  1:1009057‑M2/3‑282 (MQ=255)
                                                                                                                                                                                                         aaCGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGa                                                                                                                                                >  2:792690/1‑207 (MQ=255)
                                                                                                                                                                                                          aCGCCCCCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGa                                                             <  1:246854/289‑1 (MQ=255)
                                                                                                                                                                                                          aCGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGa                                                                                                                                                                                                                                                                         >  2:1011943/1‑86 (MQ=255)
                                                                                                                                                                                                               aCCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCCGCCGCAGTCGATTAAAATTTCCCCGCCATCTTTGTAATAACGCCCAGACAGCg                                                        >  2:2572021/1‑289 (MQ=255)
                                                                                                                                                                                                                 cacccgT‑TTGAATTTTTGGCTCGAG‑ACAGTTGTCACCCCAGCCAAGAACCGGCACGACAACCGATTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAATTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGt                                                       <  2:1113446/284‑1 (MQ=255)
                                                                                                                                                                                                                     tGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGa                                                                                                                                                                                       >  2:115941/1‑156 (MQ=255)
                                                                                                                                                                                                                     tGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCg                                                                                                                          >  1:421224/1‑217 (MQ=255)
                                                                                                                                                                                                                     tGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTAcgcg                                                  >  2:835972/1‑289 (MQ=255)
                                                                                                                                                                                                                       t‑ttG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTtct                                                                                                                                <  1:1902342/209‑1 (MQ=255)
                                                                                                                                                                                                                       t‑ttG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGtt                                                >  2:2433391/1‑288 (MQ=255)
                                                                                                                                                                                                                       t‑ttG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTg                                                <  2:460646/289‑1 (MQ=255)
                                                                                                                                                                                                                               ttttGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCtt                                          >  1:1648988/1‑289 (MQ=255)
                                                                                                                                                                                                                                   ggCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTg                                                >  2:1457491/1‑279 (MQ=255)
                                                                                                                                                                                                                                         aGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGt                                <  2:2381834/289‑1 (MQ=255)
                                                                                                                                                                                                                                           gctggttcccACCGCGGCAGAG‑ACCGACACGACCAACGGGTTGCCGAA‑CGGGCGTTAGGCCTGAGTG‑TCCGAAACCGGAATACGGAAGTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGt                                <  2:359308/279‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCCACCGCGGCAAAG‑ACCGGCCCGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATaa                        >  1:593262/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                  aCCGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAAcc          <  1:835972/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccGGCACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGa        <  1:2433391/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cACGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                       <  1:334202/221‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aCGACCACCGAGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGt  <  1:553072/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |||                                                                                                                                                                                                                                                                                        
AGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCTTAGTTATCGACTACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGT‑TTG‑ATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAG‑ACCGGCACGACCACGCTGTTACCGAA‑CTGGCGATAGGCCTGAGTG‑TCGCTAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGT  >  CP042583/1668261‑1668810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A