Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,674,584 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,674,5840GC99.7% 877.8 / NA 366intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (192/173);  minor base T (1/0);  total (193/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,674,5850CG100.0% 877.2 / NA 366intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (193/173);  total (193/173)
*CP0425831,674,5860TA100.0% 886.1 / NA 366intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (193/173);  total (193/173)

Only 100 of 366 total aligned reads displayed.

TGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGGCTATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGGCTTTTTTCTGGGCTATATCTAATGGCATTAACAATAAGATTGCTTAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCACTAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGGCTGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGGCTAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATA  >  CP042583/1674305‑1674865
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
  caGCCCTATGTTAATTTCTTTGCTGTAACGTAATCAGGACAACATTATGCTTATTGGGATAGTGATACGTAGCACTTCCCCCATTCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGGCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAAt                                                                                                                                                                                                                                                                                <  2:2976181/288‑1 (MQ=255)
          tGTTAATTTCTTTGCGGTAACGTAATACGGAAAACATTATGCTTATTGAGATAGTGATACGTATCACTTCCCCCTTGCAATTCGGCAATCGCTTTGACTAAAGAAAGGACTAGTCCCTGACATAGGGATTGACGAGAATTATCTCCCCGCCAAAATCTACGGACGAGCTCTTCCGTCTCATTAATTTTCGTTCCAGGCCTGCCGAGAACAATATTAAGTTAGCTGTTGTTATCAAGAAAACTGGATATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTaa                                                                                                                                                                                                                                                                        >  2:1706026/1‑289 (MQ=255)
                      tGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCAtt                                                                                                                                                                                                                                                                          >  2:1348938/1‑275 (MQ=255)
                              cGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACAtt                                                                                                                                                                                                                                                    <  2:1940824/289‑1 (MQ=255)
                                        aaCACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTgtagta                                                                                                                                                                                                                                          >  1:955049/1‑289 (MQ=255)
                                        aaCACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATAAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTgtagta                                                                                                                                                                                                                                          >  1:2094580/1‑289 (MQ=255)
                                               tATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAAtttt                                                                                                                                                                                                                                   <  1:1988426/289‑1 (MQ=255)
                                                   cTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATcc                                                                                                                                                                                                                               >  2:190387/1‑289 (MQ=255)
                                                      aTTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCa                                                                                                                                                                                                                            >  1:2040298/1‑289 (MQ=255)
                                                       ttGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCaa                                                                                                                                                                                                                           >  1:3069730/1‑289 (MQ=255)
                                                            aTAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGAtt                                                                                                                                                                                                                      >  2:3064844/1‑289 (MQ=255)
                                                            aTAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTGTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGAtt                                                                                                                                                                                                                      >  2:1225356/1‑289 (MQ=255)
                                                              aGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGAttt                                                                                                                                                                                                                     >  2:94593/1‑288 (MQ=255)
                                                                     cGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGAtt                                                                                                                                                                                                              >  2:2517849/1‑288 (MQ=255)
                                                                        aGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTgtagt                                                                                                                                                                                                                                           >  2:3154333/1‑256 (MQ=255)
                                                                        aGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTa                                                                                                                                                                                                                                  >  1:1448039/1‑265 (MQ=255)
                                                                        aGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTa                                                                                                                                                                                                                                  <  2:1448039/265‑1 (MQ=255)
                                                                          cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGAtt                                                                                                                                                                                                                                                              <  1:543601/235‑1 (MQ=255)
                                                                          cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        >  1:2862418/1‑289 (MQ=255)
                                                                          cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        >  1:2618526/1‑289 (MQ=255)
                                                                          cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        >  2:1532139/1‑289 (MQ=255)
                                                                           aCTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        <  1:1261675/288‑1 (MQ=255)
                                                                               cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGa                                                                                                                                                                                                                >  2:1590363/1‑276 (MQ=255)
                                                                                  ccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCAtt                                                                                                                                                                                                                                                                          >  2:1260236/1‑215 (MQ=255)
                                                                                  ccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTaa                                                                                                                                                                                                >  1:2439364/1‑289 (MQ=255)
                                                                                     tGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTg                                                                                                                                                                                                                                               >  2:1109217/1‑239 (MQ=255)
                                                                                     tGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGc                                                                                                                                                                                             >  2:2072289/1‑289 (MQ=255)
                                                                                          ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGAtt                                                                                                                                                                                                                                                              <  1:1567930/219‑1 (MQ=255)
                                                                                            cGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAAtc                                                                                                                                                                                        >  1:698996/1‑287 (MQ=255)
                                                                                                aaTCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCttt                                                                                                                                                                                   <  1:1917802/288‑1 (MQ=255)
                                                                                                 aTCGCTTTGACTAAGGAAAGGCCTAGTCACTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGCATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtct                                                                                                                                                                                 <  2:1816780/289‑1 (MQ=255)
                                                                                                 aTCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtct                                                                                                                                                                                 <  1:1238101/289‑1 (MQ=255)
                                                                                                 aTCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtct                                                                                                                                                                                 <  1:1873544/289‑1 (MQ=255)
                                                                                                  tCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTtct                                                                                                                                                                                 <  2:2089127/288‑1 (MQ=255)
                                                                                                         gACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         <  2:2640096/289‑5 (MQ=255)
                                                                                                         gACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         <  2:600252/289‑5 (MQ=255)
                                                                                                                  aagGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTAtt                                                                                                                                                                  <  1:2072289/287‑1 (MQ=255)
                                                                                                                    ggCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        <  1:3127980/247‑1 (MQ=255)
                                                                                                                            cccTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGATCATTAATTTTCGTTAAAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCca                                                                                                                                                      <  1:9255/289‑1 (MQ=255)
                                                                                                                                gACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCAtt                                                                                                                                                                                                                                                                          <  2:577964/169‑1 (MQ=255)
                                                                                                                                gACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCAtt                                                                                                                                                                                                                                                                          >  1:577964/1‑169 (MQ=255)
                                                                                                                                 aCCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         >  2:2861193/1‑261 (MQ=255)
                                                                                                                                    tACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAAttt                                                                                                                                              >  1:1814325/1‑289 (MQ=255)
                                                                                                                                     aCGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCa                                                                                                                                                                                                                            <  2:2469702/210‑1 (MQ=255)
                                                                                                                                     aCGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAAttt                                                                                                                                              >  1:1635354/1‑288 (MQ=255)
                                                                                                                                     aCGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAAttt                                                                                                                                              >  1:1027736/1‑288 (MQ=255)
                                                                                                                                         aaTGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGa                                                                                                                                          <  2:752256/288‑1 (MQ=255)
                                                                                                                                           tgacgcgaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACt                                                                                                                                        >  2:3309331/9‑288 (MQ=255)
                                                                                                                                           tgacgcgaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACt                                                                                                                                        >  2:1281141/9‑288 (MQ=255)
                                                                                                                                             acgcgaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAAt                                                                                                                                                                                                                                                                    <  1:757926/156‑1 (MQ=255)
                                                                                                                                             acacgaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACtt                                                                                                                                       >  1:3190255/7‑287 (MQ=255)
                                                                                                                                              cgctaattatCTCCCCGCCAAAATCTACGGAAGGGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        <  2:1793904/216‑1 (MQ=255)
                                                                                                                                              cgcgaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTc                                                                                                                                                                                                        >  1:1793904/6‑221 (MQ=255)
                                                                                                                                              cgcgaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTcc                                                                                                                                     <  1:3284634/283‑1 (MQ=255)
                                                                                                                                                 gaattatCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTa                                                                                                                                  <  2:1375418/286‑1 (MQ=255)
                                                                                                                                                  aattattTCCCCAGCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTAtt                                                                                                                                <  2:441523/288‑1 (MQ=255)
                                                                                                                                                             cGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTa                                                                                                                                                                                                                                                                                  >  1:2291734/1‑132 (MQ=255)
                                                                                                                                                             cGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaaag                                                                                                                                                                       >  1:295016/1‑233 (MQ=255)
                                                                                                                                                             cGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaaag                                                                                                                                                                       <  1:1966468/239‑7 (MQ=255)
                                                                                                                                                             cGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         >  1:1195402/1‑233 (MQ=255)
                                                                                                                                                               ccAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAgcgag                                                                                                                   <  2:2018060/289‑5 (MQ=255)
                                                                                                                                                                        aCGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCC‑TATTGAGAGAAAGCGAGTCCAGTTTCa                                                                                                         <  1:3256463/289‑1 (MQ=255)
                                                                                                                                                                             agagTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCa                                                                                                      <  2:534747/288‑1 (MQ=255)
                                                                                                                                                                               agTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGAttcgat                                                                                                                                                                                                                                                          <  1:2695376/138‑5 (MQ=255)
                                                                                                                                                                                           tCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTa                                                                                        <  1:496087/288‑1 (MQ=255)
                                                                                                                                                                                                                  ggCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         <  2:1193249/184‑5 (MQ=255)
                                                                                                                                                                                                                   gCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaaag                                                                                                                                                                       <  2:2614107/185‑7 (MQ=255)
                                                                                                                                                                                                                   gCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACa                                                                                                 <  1:2750379/255‑1 (MQ=255)
                                                                                                                                                                                                                   gCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACa                                                                                                 >  2:2750379/1‑255 (MQ=255)
                                                                                                                                                                                                                   gCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCaa                                                                           <  2:1874935/277‑1 (MQ=255)
                                                                                                                                                                                                                    cGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGt                                                               <  1:1317635/288‑1 (MQ=255)
                                                                                                                                                                                                                        atCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTtc                                                             >  1:1369798/1‑286 (MQ=255)
                                                                                                                                                                                                                         tCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTtc                                                             >  1:2341653/1‑285 (MQ=255)
                                                                                                                                                                                                                             tatTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaaag                                                                                                                                                                       >  1:248122/1‑169 (MQ=255)
                                                                                                                                                                                                                             tatTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCa                                                                                                      <  2:1975766/240‑1 (MQ=255)
                                                                                                                                                                                                                             tatTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAg                                                                    <  1:1771650/274‑1 (MQ=255)
                                                                                                                                                                                                                              atTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCTCTGGCAAGAAACAGTATGTTCTCTGTTac                                                     >  2:3244305/1‑287 (MQ=255)
                                                                                                                                                                                                                                  aGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAAc                                                                      <  2:1774985/267‑1 (MQ=255)
                                                                                                                                                                                                                                       gatGTTGGTAGAAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCgcgaaata                                           >  1:2217285/3‑282 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         <  2:3259824/163‑5 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGTTATTCAAGATAGTCCAACAAATTTTCGCCTGCCTTATGGAGAGAAAGCGAGTCCAGCTTCACCAAAACATGGTTTTTATCTGCCCTGGCAAGAAACAGTATTTTCTCTGTTAACCgcgaatta                                           >  2:2052280/1‑282 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTc                                                                                                                                            >  2:312039/1‑192 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAAc                                                                      <  2:1014538/262‑1 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGt                                                                   >  1:1008743/1‑265 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCgcgaaata                                           >  1:2563844/1‑282 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCgcgaaata                                           >  2:2238754/1‑282 (MQ=255)
                                                                                                                                                                                                                                       gCTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCgcgaaata                                           >  1:36662/1‑282 (MQ=255)
                                                                                                                                                                                                                                        cTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGt                                                                   <  2:64571/264‑1 (MQ=255)
                                                                                                                                                                                                                                        cTGTTGGTATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGt                                                                   >  1:64571/1‑264 (MQ=255)
                                                                                                                                                                                                                                               tATCAAGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAg                                   <  2:1635354/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   aaGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGa                                                                                                                                          <  1:3048723/182‑1 (MQ=255)
                                                                                                                                                                                                                                                   aaGAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGctct                               >  1:2917108/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     gAAAACTGGTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTctgaa                                                                                                                                                                         <  2:370765/149‑5 (MQ=255)
                                                                                                                                                                                                                                                             gTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACt                                                                                                                                        >  2:2934352/1‑174 (MQ=255)
                                                                                                                                                                                                                                                             gTTATATGAATACGCGATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTAtt                                                                                                                                >  1:341632/1‑182 (MQ=255)
                                                                                                                                                                                                                                                                         cgcgatttttCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGttt         >  1:1101536/6‑289 (MQ=255)
                                                                                                                                                                                                                                                                            gatttttCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGt                                                               >  2:2340488/3‑232 (MQ=255)
                                                                                                                                                                                                                                                                            gatttttCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGtttttt      >  1:1132140/3‑289 (MQ=255)
                                                                                                                                                                                                                                                                            gatttttCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGtttttt      <  1:1672152/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tttCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTCCCGGCAATTGTTTTTTGata  <  2:823160/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
TGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGGCTATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGAATACGGCTTTTTTCTGGGCTATATCTAATGGCATTAACAATAAGATTGCTTAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCACTAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGGCTGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGGCTAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATA  >  CP042583/1674305‑1674865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A