Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,969,243 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,969,2430GC99.7% 684.2 / NA 290intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (138/151);  minor base A (0/1);  total (138/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,969,2440CG98.6% 668.4 / NA 290intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base G (134/151);  total (138/152)
Fisher's exact test for biased strand distribution p-value = 5.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,969,2450TA99.3% 685.4 / NA 290intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (138/149);  minor base G (0/2);  total (138/152)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CCGGAGCACGCTGACTCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGGCTACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATGCTGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGGCTGTGTAACCAGCGCCAGCTTTCCAGCATG  >  CP042583/1968966‑1969522
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                     
ccGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGaa                                                                                                                                                                                                                                                                              >  2:139852/1‑289 (MQ=255)
 cGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAAc                                                                                                                                                                                                                                                                             <  2:2211307/289‑1 (MQ=255)
    aGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACAt                                                                                                                                                                                                                                                                           <  1:658589/288‑1 (MQ=255)
      cACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATgg                                                                                                                                                                                                                                                                         <  2:1321562/288‑1 (MQ=255)
           tgtgacAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACAt                                                                                                                                                                                                                                                                           >  2:3090870/6‑281 (MQ=255)
           tgtgacAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGCCGGCTGGTTTTTACCTTCCCTCCAGAAGGTGAACATGGTGTCaa                                                                                                                                                                                                                                                                   >  2:137696/6‑289 (MQ=255)
                   ttCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCCCGCCTGCGGGGAGTTCCAGACCGGCGCCCAGGCCAACGGCACTTGCGAACGGTGCGCAGCCCGCCGGTTGTTACCTTACATCGAGAAGGTGAAAATGCGGTCAatataccga                                                                                                                                                                                                                                                           >  2:1116705/1‑284 (MQ=255)
                         gCTGCCAAACATGTGGTAATCCGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGGGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGa                                                                                                                                                                                                                                                     <  2:1895852/289‑1 (MQ=255)
                           tGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcg                                                                                                                                                                                                                                                   <  2:2670732/289‑1 (MQ=255)
                           tGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcg                                                                                                                                                                                                                                                   <  1:2557169/289‑1 (MQ=255)
                             ccAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCGGGTTGTTACCTTCCATCGAGAAGGGGAACATGGGGTCAATATCCTGCGGCTGAcgcg                                                                                                                                                                                                                                                 >  2:3011/1‑289 (MQ=255)
                                     gtggtAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGa                                                                                                                                                                                                                                         >  1:308101/1‑289 (MQ=255)
                                     gtggtAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGa                                                                                                                                                                                                                                         >  1:2184342/1‑289 (MQ=255)
                                       ggtAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAAc                                                                                                                                                                                                                                       >  1:918636/1‑289 (MQ=255)
                                        gtaagccGGCGCGATACGCCATTTCCCTGCCTGCTGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCGGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAAc                                                                                                                                                                                                                                       <  2:2036835/282‑1 (MQ=255)
                                          aaTACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAAc                                                                                                                                                                                                                                       >  1:2080090/1‑286 (MQ=255)
                                                gcgcGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATAtt                                                                                                                                                                                                                              >  1:2044767/1‑289 (MQ=255)
                                                 cgcgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATgg                                                                                                                                                                                                                                                                         <  2:1997501/245‑1 (MQ=255)
                                                 cgcgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcg                                                                                                                                                                                                                                                   >  2:665864/1‑267 (MQ=255)
                                                   cgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAAc                                                                                                                                                                                                                                                                             <  1:3006228/239‑1 (MQ=255)
                                                   cgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGgc                                                                                                                                                                                                                           >  1:851378/1‑289 (MQ=255)
                                                      tACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGgcgcg                                                                                                                                                                                                                        <  1:516407/289‑1 (MQ=255)
                                                                cccGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTg                                                                                                                                                                                                                             <  1:468563/274‑1 (MQ=255)
                                                                  cGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACg                                                                                                                                                                                                            <  2:1678413/289‑1 (MQ=255)
                                                                   gTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGc                                                                                                                                                                                                                  <  1:2616578/282‑1 (MQ=255)
                                                                   gTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGa                                                                                                                                                                                                           <  1:139852/289‑1 (MQ=255)
                                                                     ccTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                                                          <  1:316945/288‑1 (MQ=255)
                                                                       tGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACa                                                                                                                                                                                                                                                                            <  2:798930/220‑1 (MQ=255)
                                                                       tGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACa                                                                                                                                                                                                                                                                            >  1:798930/1‑220 (MQ=255)
                                                                          ggCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCCCGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGt                                                                                                                                                                                                    <  1:1876069/289‑1 (MQ=255)
                                                                             ggggaGCGTGCCGGTACGGTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTaa                                                                                                                                                                                                  <  2:3268032/284‑1 (MQ=255)
                                                                                     tGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACa                                                                                                                                                                                                                                                                            <  2:2629913/206‑1 (MQ=255)
                                                                                                 ggTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCGAGt                                                                                                                                                                             >  1:971909/1‑289 (MQ=255)
                                                                                                  gTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACg                                                                                                                                                                                                                                      >  1:437738/1‑231 (MQ=255)
                                                                                                  gTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGTTCTTGGCGCGCATGGCGGTACGCCCGCTGTAACGGGGCGGTTTCCGGGCCAGtg                                                                                                                                                                            >  2:2554903/1‑288 (MQ=255)
                                                                                                      aGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTg                                                                                                                                                                                             <  1:3305756/268‑1 (MQ=255)
                                                                                                          aTCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCt                                                                                                                                                                                                                                                             <  2:2082984/200‑1 (MQ=255)
                                                                                                          aTCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGc                                                                                                                                                                                                                                     <  2:887905/224‑1 (MQ=255)
                                                                                                          aTCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGccc                                                                                                                                                                     >  1:26386/1‑288 (MQ=255)
                                                                                                            ccAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACg                                                                                                                                                                                                            >  2:2366499/1‑247 (MQ=255)
                                                                                                            ccAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACg                                                                                                                                                                                                            <  1:2366499/247‑1 (MQ=255)
                                                                                                                aCCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGaa                                                                                                                                                                                                                                        <  2:464044/215‑1 (MQ=255)
                                                                                                                aCCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGc                                                                                                                                                                                            <  2:1848367/259‑1 (MQ=255)
                                                                                                                aCCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGc                                                                                                                                                                                            >  1:1848367/1‑259 (MQ=255)
                                                                                                                  cATTTTCGCAGGTCTCAAACAGACACACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAAGTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACg                                                                                                                                                            <  2:1143870/289‑1 (MQ=255)
                                                                                                                    ttttCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGAGGTTCACGGGCCAGTTTCTGCCCACGAATACGGa                                                                                                                                                          >  1:1143870/1‑289 (MQ=255)
                                                                                                                     tttCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGaa                                                                                                                                                         >  1:2436585/1‑289 (MQ=255)
                                                                                                                            ggTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTAc                                                                                                                                                                                                             >  1:2505686/1‑230 (MQ=255)
                                                                                                                                       gACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGa                                                                                                                                                                                                                                                     >  2:1045120/1‑179 (MQ=255)
                                                                                                                                       gACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCGGGCgcagc                                                                                                                                       >  1:1727317/1‑289 (MQ=255)
                                                                                                                                        acgcacgcCCGGATCGCCAAAGCGCAGTTTGCCGCCCGCTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgcagc                                                                                                                                       <  2:1240448/288‑1 (MQ=255)
                                                                                                                                         cgcacgcCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgcagca                                                                                                                                      <  1:1976004/288‑1 (MQ=255)
                                                                                                                                         cgcacgcCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgcagc                                                                                                                                       <  2:2028632/287‑1 (MQ=255)
                                                                                                                                                ccGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGAt                                                                                                                              >  1:1293140/1‑289 (MQ=255)
                                                                                                                                                        ccAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACg                                                                                                                                                                                                            <  2:2615980/203‑1 (MQ=255)
                                                                                                                                                        ccAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTaa                                                                                                                                                                                                  <  1:2942276/213‑1 (MQ=255)
                                                                                                                                                                    ttGCCGCCCACTTCGTCCTGGAATTTGTGCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGGGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                           <  1:3245046/288‑1 (MQ=255)
                                                                                                                                                                        cgccCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGc                                                                                                      <  1:95126/289‑1 (MQ=255)
                                                                                                                                                                               ttCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCccaac                                                                                                >  2:2322898/1‑288 (MQ=255)
                                                                                                                                                                               ttCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCacaaca                                                                                               >  1:6973/1‑289 (MQ=255)
                                                                                                                                                                                    ccTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgcagc                                                                                                                                       <  2:713130/244‑1 (MQ=255)
                                                                                                                                                                                           tttGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATcaca                                                                                   <  1:137696/289‑1 (MQ=255)
                                                                                                                                                                                             tGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTc                                                                                          <  1:2338592/280‑1 (MQ=255)
                                                                                                                                                                                                tCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTc                                                                              <  1:3039665/289‑1 (MQ=255)
                                                                                                                                                                                                           ggggAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtc                                                                    <  1:2098072/288‑1 (MQ=255)
                                                                                                                                                                                                                gTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGTGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtc                                                               <  1:1334509/288‑1 (MQ=255)
                                                                                                                                                                                                                   ccAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACt                                                           >  1:1001365/1‑289 (MQ=255)
                                                                                                                                                                                                                           gcgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACtt                                                          <  2:932060/282‑1 (MQ=255)
                                                                                                                                                                                                                           gcgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGGCGCATCCGGCGCAGCATCCTTGATACCTGCCAGTTCCGGGATTTTCCCCACAAAAGCGTCAATCACATGGTCAAGCTGCCTCCAGTCCCCTTCCCGGc                                                    >  2:1356621/1‑288 (MQ=255)
                                                                                                                                                                                                                            cgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtc                                                               <  2:2142692/276‑1 (MQ=255)
                                                                                                                                                                                                                            cgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCt                                                   <  1:2615300/288‑1 (MQ=255)
                                                                                                                                                                                                                                cAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTcagca                                              <  1:2018253/289‑1 (MQ=255)
                                                                                                                                                                                                                                             cttgcgaaCGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGgcgc                                                                                                                                          <  1:628302/177‑1 (MQ=255)
                                                                                                                                                                                                                                             cttgcgaaCGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGgcgc                                                                                                                                          >  2:628302/8‑184 (MQ=255)
                                                                                                                                                                                                                                                gcgaaCGGTGCGGAGTCGGGTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAgcga                              <  1:1045508/285‑4 (MQ=255)
                                                                                                                                                                                                                                                    aCGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAgcgagtg                           <  1:1677970/288‑7 (MQ=255)
                                                                                                                                                                                                                                                     cGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTATGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtc                                                                    <  1:11192/246‑1 (MQ=255)
                                                                                                                                                                                                                                                     cGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagt                                                                >  1:2125972/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        tGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTaa                                                                                                                                                                                                  >  1:76208/1‑117 (MQ=255)
                                                                                                                                                                                                                                                        tGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGGTCACGGGCCAGTTTCTGCCTACGAATACGGAATGTGGCATCCGGCGCAGCATCTTTGATCCCTGCCAGATCCGGGATTTTCGCCACAACAGCGTTAATCCCATGGTCGATCTTCGTCCGGTCCACTTCCCGGTTCAGCATGGCGCTGTTCAgcgagggtaacc                      >  2:299381/1‑278 (MQ=255)
                                                                                                                                                                                                                                                        tGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGAGCGGTTCACGGGCCAGTTTCTGCCCACGAAGACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCg                                                                                            >  1:2145366/1‑219 (MQ=255)
                                                                                                                                                                                                                                                                cGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGATGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGc                                                                                                                                                                                 >  2:1797111/1‑126 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATg                                                                                                                                                                                                                                          >  2:2721312/1‑67 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATg                                                                                                                                                                                                                                          <  1:2721312/67‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATc                                                                                                                                    <  2:1921555/169‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATc                                                                                                                                    >  1:1921555/1‑169 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGa                                                                            >  2:966266/1‑225 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtcca                                                             >  2:2823538/1‑240 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtcca                                                             <  1:2823538/240‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTcagca                                              <  2:121151/255‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTcagca                                              >  1:121151/1‑255 (MQ=255)
                                                                                                                                                                                                                                                                  gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCGCAGCAGGGTGCTGTGGAGCGAGGGTAACCCGCGCCAGCt            >  2:160893/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    tGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGt                                                                                           >  1:1624362/1‑208 (MQ=255)
                                                                                                                                                                                                                                                                      gttgttACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTc                                                                                          <  2:305320/207‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gttACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTGCACGGCCCACACGGGTGCTGTGCAGCGTGTGTAACCAGCGCCCGCTTTCCAGc     >  2:2871386/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           tACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCAt   <  2:496240/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCAt   <  1:1828848/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aCCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCCCTTCACGGCTCAGCAGGGTGCTGTTCAGCGAGTGTAACCAGGCCCAGCTCTCCAGCATg  >  2:458358/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             ccTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGc                                        >  2:2450218/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                             ccTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATg  >  1:2265112/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                     
CCGGAGCACGCTGACTCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGGCTACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATGCTGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGGCTGTGTAACCAGCGCCAGCTTTCCAGCATG  >  CP042583/1968966‑1969522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A