Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,487,849 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,487,8490AT99.5% 489.3 / NA 208intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (98/109);  minor base G (1/0);  total (99/109)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,487,8500CG100.0% 489.4 / NA 208intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (99/109);  total (99/109)
*CP0425831,487,8510TA99.5% 489.0 / NA 208intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (99/108);  total (99/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

GACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCACTAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGGCTCCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGACTGGAGCTTTTCGTATTGCCACTTTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGACTCGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAAT  >  CP042583/1487569‑1488128
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
gACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCt                                                                                                                                                                                                                                                                                 >  2:182803/1‑289 (MQ=255)
  ccGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGTCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCtt                                                                                                                                                                                                                                                                                <  1:268307/288‑1 (MQ=255)
     aaTTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCg                                                                                                                                                                                                                                                                            >  1:1531895/1‑289 (MQ=255)
     aaTTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCg                                                                                                                                                                                                                                                                            >  1:473020/1‑289 (MQ=255)
     aaTTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTAAGCCGTAAGTGAGGAGCTTTTCg                                                                                                                                                                                                                                                                            >  1:1257406/1‑289 (MQ=255)
     aaTTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGGGTCCATACAAGGTCAGGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCg                                                                                                                                                                                                                                                                            >  2:1545681/1‑289 (MQ=255)
     aaTACAACGCAGAAACTGCTTACCAGGCCATTCAAAAACCAACGACCATAACCCGCGATAAACCCATCGCACTCTCGATAGACGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCg                                                                                                                                                                                                                                                                            <  2:1307974/289‑1 (MQ=255)
       ttCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACGCATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGACGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTa                                                                                                                                                                                                                                                                          <  2:859376/289‑1 (MQ=255)
          aaaTCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTg                                                                                                                                                                                                                                                                       <  1:1024478/289‑1 (MQ=255)
           aaGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGGCATGGTGGTCTCCGCCGTATGTGAGGAGCTTTTCGTATTg                                                                                                                                                                                                                                                                       >  2:791576/1‑288 (MQ=255)
                gAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGcctga                                                                                                                                                                                                                                                                  <  2:1372087/288‑4 (MQ=255)
                     tgtgTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGc                                                                                                                                                                                                                                                            <  1:849559/289‑1 (MQ=255)
                        gtcccGGGCCCTTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAg                                                                                                                                                                                                                                                         <  2:385904/286‑1 (MQ=255)
                          aCCGGCCCATTCCATAACTGACGACCATAGCCCGCGCTCAACACATAGCGCTCTCTATATCCGTTATAACGGTCTGTCACCAAGCTTACCTGAACAAATAAATAGTCATAATCAGTTAAATTAATACGGCTCCGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTCCTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                                                                                                                                                                                       <  2:281563/289‑1 (MQ=255)
                              ggCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGatat                                                                                                                                                                                                                                                     >  2:748918/1‑287 (MQ=255)
                                ccATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCt                                                                                                                                                                                                                                                  <  1:1850065/288‑1 (MQ=255)
                                 cATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGCTTGTGCAAGATATCCttc                                                                                                                                                                                                                                                >  1:240751/1‑287 (MQ=255)
                                   ttCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGcctga                                                                                                                                                                                                                                                                  >  1:1867254/1‑266 (MQ=255)
                                             gacgacCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGcctga                                                                                                                                                                                                                                                                  <  2:1487327/259‑4 (MQ=255)
                                                            cgGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTTCGTTCAGATTACCTTCGAAAGGtttttt                                                                                                                                                                                                                     >  1:444684/1‑289 (MQ=255)
                                                            cgGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtttttt                                                                                                                                                                                                                     >  2:571313/1‑289 (MQ=255)
                                                                    caaTCGCGCACTCGATAGCCATTATAACAGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGGCATATTCAGTTAAATTAAAACGGCTACGCCCGCCCGCTGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTCCTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTgg                                                                                                                                                                                                             <  2:906937/287‑1 (MQ=255)
                                                                     catggggcTCTCGATAGCCGTTATAACGGTCGGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGc                                                                                                                                                                                                            <  2:700686/283‑1 (MQ=255)
                                                                        cgcgCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAgg                                                                                                                                                                                                         >  1:49607/1‑289 (MQ=255)
                                                                         gcgcTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        <  2:596106/289‑1 (MQ=255)
                                                                         gcgcTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        <  1:170782/289‑1 (MQ=255)
                                                                         gcgcTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        <  1:1352551/289‑1 (MQ=255)
                                                                         gcgcTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        <  1:1479344/289‑1 (MQ=255)
                                                                         gaactctGGATAGCCGTGATAACGGTCGGTCAGCCAACTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        <  2:498150/286‑1 (MQ=255)
                                                                                         ttATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCATATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATg                                                                                                                                                                                               >  1:166625/1‑282 (MQ=255)
                                                                                         ttATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCATATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATg                                                                                                                                                                                               <  2:166625/282‑1 (MQ=255)
                                                                                         ttATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTaa                                                                                                                                                                                        >  2:1261403/1‑289 (MQ=255)
                                                                                                 gTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa                                                                                                                                                                                >  1:252920/1‑289 (MQ=255)
                                                                                                 gTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa                                                                                                                                                                                >  1:1510823/1‑289 (MQ=255)
                                                                                                 gTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCACGACAGTAAAAACGGaa                                                                                                                                                                                >  2:1277808/1‑289 (MQ=255)
                                                                                                   cTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAgcg                                                                                                                                                                                                                 >  2:1161434/1‑254 (MQ=255)
                                                                                                    tGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatca                                                                                                                                                                             >  1:622472/1‑289 (MQ=255)
                                                                                                           cagcTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCgg                                                                                                                                                                         <  1:1156294/286‑1 (MQ=255)
                                                                                                                tGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCa                                                                                                                                                                 <  2:730277/289‑1 (MQ=255)
                                                                                                                      aCCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatcatc                                                                                                                                                                           >  2:356103/1‑273 (MQ=255)
                                                                                                                      aCCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatcatc                                                                                                                                                                           <  1:356103/273‑1 (MQ=255)
                                                                                                                      aCCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAgt                                                                                                                                                           >  1:90398/1‑288 (MQ=255)
                                                                                                                                   tCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATg                                                                                                                                               >  2:836324/1‑288 (MQ=255)
                                                                                                                                        aTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGcccccc                                                                                                                                         <  1:881546/289‑1 (MQ=255)
                                                                                                                                             ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACa                                                                                                                                                 <  1:1437687/276‑1 (MQ=255)
                                                                                                                                             ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCaa                                                                                                                                    >  2:694703/1‑289 (MQ=255)
                                                                                                                                             ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCa                                                                                                                                     >  2:628016/1‑288 (MQ=255)
                                                                                                                                              taaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTTGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGcc                                                                                                                                      <  1:1806600/286‑1 (MQ=255)
                                                                                                                                                    aaaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg                                                                                                                             <  1:1270161/289‑1 (MQ=255)
                                                                                                                                                    aaaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg                                                                                                                             <  1:133793/289‑1 (MQ=255)
                                                                                                                                                    aaaaCGGCTACGCCCGCCCCCCGAAGATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTAATGGCGTTCCCCCACAGCGACCAGGCAGTGGTGGGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg                                                                                                                             <  2:444684/289‑1 (MQ=255)
                                                                                                                                                      aaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTAGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGAt                                                                                                                           <  1:598907/289‑1 (MQ=255)
                                                                                                                                                        cGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATgg                                                                                                                         <  1:944849/289‑1 (MQ=255)
                                                                                                                                                           cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                                                                                                                                                                                       >  2:1500383/1‑160 (MQ=255)
                                                                                                                                                           cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAg                                                                                                                      >  1:1590271/1‑289 (MQ=255)
                                                                                                                                                              cgcccggccgccGCATATTTTTCTGGAGAACGCGCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGaa                                                                                                                   <  2:1239869/289‑1 (MQ=255)
                                                                                                                                                              cgcccgcccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGaa                                                                                                                   <  1:694703/289‑1 (MQ=255)
                                                                                                                                                                    cccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtttt                                                                                                                                                                                                                       >  1:796060/1‑183 (MQ=255)
                                                                                                                                                                    cccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        >  2:380720/1‑198 (MQ=255)
                                                                                                                                                                    cccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                                                                                                                                                                                        <  1:380720/198‑1 (MQ=255)
                                                                                                                                                                    cccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCt                                                                                                             >  2:309814/1‑289 (MQ=255)
                                                                                                                                                                              atTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCtt                                                                                                   <  1:773011/289‑1 (MQ=255)
                                                                                                                                                                                ttttctgaaGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCa                                                                                                 <  2:252920/281‑1 (MQ=255)
                                                                                                                                                                                ttttctgaaGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCa                                                                                                 <  2:1580394/281‑1 (MQ=255)
                                                                                                                                                                                   tctgaaGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCAttt                                                                                              >  1:1094481/6‑289 (MQ=255)
                                                                                                                                                                                           agactcatacgtggaagaaGTCTTACTGGCGTTCCCCCACAGCGACCAGGGAGGGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGGGCTTTTCGTATTGGCTGATTGTGCAAGATATCCTGCGTTCAGGTTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGAGCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAaga                                                                                       <  2:1011569/275‑1 (MQ=255)
                                                                                                                                                                                            cGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCaa                                                                                                                                    <  2:884147/242‑1 (MQ=255)
                                                                                                                                                                                            cGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGt                                                                                     >  2:1712349/1‑289 (MQ=255)
                                                                                                                                                                                                  tcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac                                                                               <  1:759990/289‑1 (MQ=255)
                                                                                                                                                                                                      tAGAAGAAGTATTACTGGCGGTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTacaaca                                                                           <  1:1400451/289‑1 (MQ=255)
                                                                                                                                                                                                         aagaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCACCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACaaaa                                                                        >  2:185546/1‑289 (MQ=255)
                                                                                                                                                                                                             agTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTCTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAag                                                                    >  1:747256/1‑289 (MQ=255)
                                                                                                                                                                                                                ttaacgggCGTTCCCCCAAAGCGCGCAGGAAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGAATGTGCAAGATATCCTGCGTTCAGATTACCGTCGAAAGGTTTTTTAGCGCTGGCAGTATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCATCATTTATCAAGAGTCCGTACAACAAAAAAAgaga                                                                 <  2:1418710/283‑1 (MQ=255)
                                                                                                                                                                                                                aTTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGc                                                                                                                        >  1:278949/1‑234 (MQ=255)
                                                                                                                                                                                                                aTTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac                                                                               <  2:83854/275‑1 (MQ=255)
                                                                                                                                                                                                                aTTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac                                                                               >  1:83854/1‑275 (MQ=255)
                                                                                                                                                                                                                aTTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAgaga                                                                 >  2:1521639/1‑289 (MQ=255)
                                                                                                                                                                                                                      ggCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATcg                                                           <  2:1094481/289‑1 (MQ=255)
                                                                                                                                                                                                                           tCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGt                                                       >  1:1208524/1‑288 (MQ=255)
                                                                                                                                                                                                                                  cagcgaccAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGaaa                                               <  1:1545681/283‑1 (MQ=255)
                                                                                                                                                                                                                                     cgcccAGGAAATGGTGGGTCCATAACAGGTCATGGTGGGGTCAGCCGTAAGTGAGGAGCTTTTCGTAGTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACt                                             <  2:90398/285‑1 (MQ=255)
                                                                                                                                                                                                                                     cgaccAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACtt                                            >  2:1815281/4‑289 (MQ=255)
                                                                                                                                                                                                                                       accAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGAACAACCCGGTCCCGGAAACtt                                            >  2:1120444/2‑287 (MQ=255)
                                                                                                                                                                                                                                             cAGTGGTGTTGCCAAACCAGGTCATAGTGGGGTCAGCCGTAAGTGAGGGGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTa                                       <  2:240751/286‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTaa                                      <  1:1833816/287‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTa                                       <  1:345173/286‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAg                                     <  1:836324/288‑1 (MQ=255)
                                                                                                                                                                                                                                                 ggtgTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATc                                 <  1:1416101/288‑1 (MQ=255)
                                                                                                                                                                                                                                                     tgtCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCgg                                                                                                                                                                         <  2:1349154/148‑1 (MQ=255)
                                                                                                                                                                                                                                                      gtCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCtt                                        <  2:1840704/276‑1 (MQ=255)
                                                                                                                                                                                                                                                      gtCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGAt                           >  1:223156/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         cATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAg                        <  1:365123/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         cATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAg                        <  1:1120444/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGCAACTTTCTTAAGGATCAAGTATTAGCGCCCCTg                >  2:1637793/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                   cATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACCAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGa              <  1:555265/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tggtgTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGt         >  2:1316831/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            gtCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACg     <  1:1496372/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              cAGCCGTAAGTGATGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGaa   <  2:473020/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGc                                                                                                                                                                                                            <  2:272262/87‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAAt  >  1:1899683/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
GACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCATAACCCGCGGTCAACACATCGCGCTCTCGATAGCCGTTATAACGGTCTGTCAGCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCACTAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGGCTCCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGACTGGAGCTTTTCGTATTGCCACTTTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGACTCGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAAT  >  CP042583/1487569‑1488128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A