Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,536,686 6 bp→CGATGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,536,6860GC99.0% 676.5 / 0.6 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base C (156/128);  minor base . (2/1);  total (158/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
*CP0425831,536,6870CG98.6% 664.6 / ‑4.9 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base G (156/128);  total (158/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*CP0425831,536,6880TA98.6% 673.4 / ‑2.1 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/2);  major base A (156/127);  minor base G (2/2);  total (158/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
*CP0425831,536,6890AT98.6% 673.0 / NA 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (156/128);  total (158/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,536,6900CG99.0% 679.8 / NA 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (156/129);  minor base T (2/1);  total (158/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,536,6910TA99.3% 682.1 / NA 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (156/129);  minor base G (1/1);  total (158/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGACTAAGTGTATTTTTGTTTAGTTCCCCACCCGTACTAACGACTTTAATTTCACTCTGAGCCAGAACATGAATCGCTTCCGCGCTGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAAGCTACTA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTACTGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAAC  >  CP042583/1536417‑1536961
                                                                                                                                                                                                                                                                             ||||||                                                                                                                                                                                                                                                                               
tGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATaaa                                                                                                                                                                                                                                                                  <  1:82081/289‑1 (MQ=255)
 gATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCGTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATaaaa                                                                                                                                                                                                                                                                 >  1:505068/1‑289 (MQ=255)
  aTAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTTCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATaaaaa                                                                                                                                                                                                                                                                >  1:1068993/1‑289 (MQ=255)
        ccTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGata                                                                                                                                                                                                                                                          >  2:359472/1‑289 (MQ=255)
        ccTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTCATTGTCGATAAAGGGTAATGCCTTGCGTGCAATACGCTGCTTCTCTTCATAAAACGATGAA‑GTGCGCCTATAAAAATGGaaa                                                                                                                                                                                                                                                          >  2:1563414/1‑287 (MQ=255)
            atgatCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTGTAAAAATGGCAATtct                                                                                                                                                                                                                                                      >  2:247851/1‑289 (MQ=255)
                     ttAGTAATTCTTCCTTGCAGTGAGAATTTATTTTTGTGTGGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAAAATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACa                                                                                                                                                                                                                                             <  2:1672107/289‑1 (MQ=255)
                          aaTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCt                                                                                                                                                                                                                                        <  2:1326596/289‑1 (MQ=255)
                          aaTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCt                                                                                                                                                                                                                                        <  2:1747132/289‑1 (MQ=255)
                           aTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATc                                                                                                                                                                                                                                           >  1:1139756/1‑285 (MQ=255)
                            ttcttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCAATTACGGTACTACTGGAATCGGCTGCCCTGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGAGATTCCCCGTCAACAttcattt                                                                                                                                                                                                                                      >  2:1303251/1‑283 (MQ=255)
                            ttcttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTGGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAGAGGGTAGTGCCTTGCGTTCAATAAGCTCCTTCGCTTCATAAAACGATGAA‑TCGCGCTTATAACAATGGATCTTCTCCGTCAACATCGCTGt                                                                                                                                                                                                                                      >  2:852345/1‑289 (MQ=255)
                              cttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCg                                                                                                                                                                                                                                          >  1:1618196/1‑283 (MQ=255)
                              cttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTAt                                                                                                                                                                                                                                    >  2:98488/1‑289 (MQ=255)
                                    tgcagtgaaaGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTaaaa                                                                                                                                                                                                                              <  1:349904/281‑1 (MQ=255)
                                          gaaaGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGca                                                                                                                                                                                                                         <  1:466162/286‑1 (MQ=255)
                                           aaaGTGTTTTTTTGTTTGTTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGcacc                                                                                                                                                                                                                       <  2:43981/288‑1 (MQ=255)
                                            aaGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGcacc                                                                                                                                                                                                                       <  2:410744/288‑1 (MQ=255)
                                                gtATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCAt                                                                                                                                                                                                                  >  2:161357/1‑289 (MQ=255)
                                                 tATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATa                                                                                                                                                                                                                 >  2:929266/1‑289 (MQ=255)
                                                           tAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGCATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATAGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTTTTCATAAAACGATGAA‑TCGCGCTTATAGAAATGGATATTCCCGGTCAACATCGCTGTATTTAAAACAGCACCACCATACGTTCTGGTc                                                                                                                                                                                                       >  2:754074/1‑289 (MQ=255)
                                                                ccccACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCaaaaa                                                                                                                                                                                                  <  1:773475/289‑1 (MQ=255)
                                                                   cACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGc                                                                                                                                                                                                >  2:1073214/1‑288 (MQ=255)
                                                                     cccgttgaaaCGACTTTATTTTCTGACTGAGCCAGAACTTGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCtt                                                                                                                                                                                             <  2:1641423/281‑1 (MQ=255)
                                                                     cccgttgaaaCGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCtt                                                                                                                                                                                             <  1:987182/281‑1 (MQ=255)
                                                                        gttgaaaCGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCa                                                                                                                                                                                           >  1:1179236/6‑288 (MQ=255)
                                                                               cGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAg                                                                                                                                                                                   <  2:787500/289‑1 (MQ=255)
                                                                               cGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAg                                                                                                                                                                                   <  1:1082772/289‑1 (MQ=255)
                                                                                                  aGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGa                                                                                                                                                                >  1:593998/1‑289 (MQ=255)
                                                                                                         aCATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGt                                                                                                                                                           <  2:949103/287‑1 (MQ=255)
                                                                                                             gAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTttcttc                                                                                                                                                     >  2:971764/1‑289 (MQ=255)
                                                                                                                             gTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAAt                                                                                                                                      <  1:700798/288‑1 (MQ=255)
                                                                                                                               tGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATccc                                                                                                                                   <  1:796273/289‑1 (MQ=255)
                                                                                                                                        aCGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAtt                                                                                                                          >  1:1461768/1‑289 (MQ=255)
                                                                                                                                           tCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAtttga                                                                                                                       <  2:607401/289‑4 (MQ=255)
                                                                                                                                                ccACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAtttgaga                                                                                                                     <  1:161357/286‑6 (MQ=255)
                                                                                                                                                  acgaCGTTCCTGTAACAATTTGGTCAATTCCATTACGGTACTCCTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTc                                                                                                                <  1:1563414/286‑1 (MQ=255)
                                                                                                                                                   cTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTc                                                                                                                >  2:740213/1‑288 (MQ=255)
                                                                                                                                                    tACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCa                                                                                                               >  2:1184970/1‑288 (MQ=255)
                                                                                                                                                         tCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTc                                                                                                                                                                                                            <  2:675808/190‑1 (MQ=255)
                                                                                                                                                         tCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTc                                                                                                                                                                                                            >  1:675808/1‑190 (MQ=255)
                                                                                                                                                         tCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCtttt                                                                                                         >  1:10020/1‑289 (MQ=255)
                                                                                                                                                         tCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCtttt                                                                                                         >  1:537853/1‑289 (MQ=255)
                                                                                                                                                         tCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGATAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCtttt                                                                                                         >  2:1226488/1‑289 (MQ=255)
                                                                                                                                                           cTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCAACTCAAATTTGAGACTGTCACCTTTTTa                                                                                                       >  2:1462352/1‑289 (MQ=255)
                                                                                                                                                            tGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTa                                                                                                       >  1:253123/1‑288 (MQ=255)
                                                                                                                                                            tGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTAt                                                                                                      <  1:929266/289‑1 (MQ=255)
                                                                                                                                                              tAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATTGTGGCTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGTTAAAAAAGCTTTCATCTTCCAGCTTCTCAATATCGTGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAATTTTGAGACTGTCACCTTTTTATCg                                                                                                    >  1:1718157/1‑289 (MQ=255)
                                                                                                                                                              tAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCg                                                                                                    >  1:1768399/1‑289 (MQ=255)
                                                                                                                                                                    tttGAGCAATTTCCTTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTTGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCa                                                                                              <  1:856828/289‑1 (MQ=255)
                                                                                                                                                                     ttGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTCGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCAACATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTACTTCGGTTACCTGAAAAATCCCACTCAACTTGGAGATTGTCCACTTTTTCTCGTTGGCa                                                                                              >  2:1408659/1‑288 (MQ=255)
                                                                                                                                                                     ttGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGt                                                                                                                 <  1:753235/269‑1 (MQ=255)
                                                                                                                                                                     ttGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCa                                                                                              >  2:529835/1‑288 (MQ=255)
                                                                                                                                                                        aGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCa                                                                                          >  2:920626/1‑289 (MQ=255)
                                                                                                                                                                             ttCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCAttt                                                                                       >  2:389298/1‑287 (MQ=255)
                                                                                                                                                                                       gTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTg                                                                              >  1:191990/1‑286 (MQ=255)
                                                                                                                                                                                       gTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTg                                                                              >  2:1906777/1‑286 (MQ=255)
                                                                                                                                                                                        tactacTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCCGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTg                                                                          >  2:153011/1‑289 (MQ=255)
                                                                                                                                                                                        tactacTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCt                                                                           <  1:947922/288‑1 (MQ=255)
                                                                                                                                                                                        tactacTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTg                                                                          >  1:906951/1‑289 (MQ=255)
                                                                                                                                                                                          ctactGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAACAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTTCTTAATTGCCTGaa                                                                        >  2:837341/1‑289 (MQ=255)
                                                                                                                                                                                          ctactGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAtttgaga                                                                                                                     <  2:1194827/244‑6 (MQ=255)
                                                                                                                                                                                           tactGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGaa                                                                        >  1:424544/1‑288 (MQ=255)
                                                                                                                                                                                             ctGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAAt                                                                       >  1:1272974/1‑287 (MQ=255)
                                                                                                                                                                                                  aTCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAtttgaga                                                                                                                     >  1:1665211/1‑231 (MQ=255)
                                                                                                                                                                                                  aTCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCt                                                                >  1:1353552/1‑289 (MQ=255)
                                                                                                                                                                                                  aTCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACCCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCt                                                                >  2:1118601/1‑289 (MQ=255)
                                                                                                                                                                                                     ggCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGcac                                                                                                                                                                                                                        >  2:1779607/1‑134 (MQ=255)
                                                                                                                                                                                                     ggCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCtttt                                                             >  2:1497614/1‑289 (MQ=255)
                                                                                                                                                                                                       cTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGcacc                                                                                                                                                                                                                       <  1:102658/133‑1 (MQ=255)
                                                                                                                                                                                                           cATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgccgcc                                                       <  1:1224681/289‑1 (MQ=255)
                                                                                                                                                                                                           cATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgccgc                                                        <  2:1061788/288‑1 (MQ=255)
                                                                                                                                                                                                             tggtggTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCAc                                                     <  1:153090/289‑1 (MQ=255)
                                                                                                                                                                                                             tggtggTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCAc                                                     <  2:1015012/289‑1 (MQ=255)
                                                                                                                                                                                                                    tttATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCACCATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTac                                               >  2:1480040/1‑288 (MQ=255)
                                                                                                                                                                                                                     ttATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTacac                                             <  2:666580/289‑1 (MQ=255)
                                                                                                                                                                                                                       aTTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACAccc                                           <  2:1272974/289‑1 (MQ=255)
                                                                                                                                                                                                                          ggcGATAAAGGGTAGTTCCTGGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGAGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTg                                        <  2:1768399/287‑1 (MQ=255)
                                                                                                                                                                                                                             gATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                                                >  1:1789357/1‑246 (MQ=255)
                                                                                                                                                                                                                              aTAAAGGGTCGTGCCTTGCCTGCAATAGGCTGATTCTCTTGCTAAAA‑CGATGAGGCTCGCTTATAAAAATGGATATTCTCCGTCAGCATCGCTGTATTTAACACAGCACCACCATAGGTTCTGGTCAAAAGGCCTTCATCTTCCTGCTTCTCGAGATCGCGGCGAATGGTTTCTTCGGTTACCTGACAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAGTCCTGTCTTTTGCCGCCACGATTACACCCCTGTAt                                     <  2:1856246/288‑1 (MQ=255)
                                                                                                                                                                                                                                     gTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTAc                             >  1:1705813/1‑289 (MQ=255)
                                                                                                                                                                                                                                        gTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGTCACGATTACACCCCTTTATCtttttccct                           >  2:1885283/1‑284 (MQ=255)
                                                                                                                                                                                                                                         tGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGcac                                                                                                                                                                                                                        <  1:554527/98‑1 (MQ=255)
                                                                                                                                                                                                                                         tGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGcac                                                                                                                                                                                                                        >  2:554527/1‑98 (MQ=255)
                                                                                                                                                                                                                                         tGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGca                                                                                                                                                                                                                         >  2:1648070/1‑97 (MQ=255)
                                                                                                                                                                                                                                         tGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGca                                                                                                                                                                                                                         <  1:1648070/97‑1 (MQ=255)
                                                                                                                                                                                                                                              tGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCTTTGGCAACCATTTGCTTAATGGCCTGAATCCTGTCTTTTGCCGCCACGATTCGACCCCTGTATCTTTTTACATCACATTa                    >  1:977269/1‑289 (MQ=255)
                                                                                                                                                                                                                                              tGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTa                    >  1:960634/1‑289 (MQ=255)
                                                                                                                                                                                                                                              tGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTa                    >  2:1176331/1‑289 (MQ=255)
                                                                                                                                                                                                                                               gCGTGCAATAAGCTGCGTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCTCCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGTTTTCTTCGGTGACCTGACAAATCCCACTCAAATTTGGGACTGTCCCCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTGGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAg                   <  2:333214/289‑1 (MQ=255)
                                                                                                                                                                                                                                                cGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAgc                  >  1:189060/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 gTGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAgcg                 <  1:1195627/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  tGCAATAAGCTGCTTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACCCCCCTGTATCTTTTTACATCACATTAgcgc                >  1:867429/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            tgctTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTTTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCg        <  1:1303251/287‑1 (MQ=255)
                                                                                                                                                                                                                                                            tgctTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCa      <  1:1088870/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            tgctTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCa      <  1:389298/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            tgctTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCa      <  2:1619284/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              ctTCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATa    >  1:635501/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               ttCTCTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCCCGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATtctctatta    >  2:713256/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                tctcTTCATAAAACGATGAA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAAc  <  1:147612/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ||||||                                                                                                                                                                                                                                                                               
TGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGACTAAGTGTATTTTTGTTTAGTTCCCCACCCGTACTAACGACTTTAATTTCACTCTGAGCCAGAACATGAATCGCTTCCGCGCTGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAAGCTACTA‑GCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTACTGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAAC  >  CP042583/1536417‑1536961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A