Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,506,537 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,506,5370AT99.1% 522.4 / NA 224intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (111/111);  minor base G (1/1);  total (112/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,506,5380CG98.2% 500.8 / NA 224intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base G (107/110);  minor base T (3/1);  total (112/112)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,506,5390TA99.1% 509.0 / NA 224intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (112/108);  minor base G (0/2);  total (112/112)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 224 total aligned reads displayed.

GATCACGACGGTTATAAAATTCTTTGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTACTGAACTTGATCCGGGCTATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGACTGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATGCTCATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGG  >  CP042583/1506258‑1506817
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                      
tggaagaagcgacaacgttaattcggacacccgccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTACTCACCAACACCACCCCCCCCCGTGAGCCAAAg                                                                                                                                                                                                                                                                                 >  2:1168286‑M2/140‑289 (MQ=255)
tggaagaagcgacaacgttaattcggacacccgccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAg                                                                                                                                                                                                                                                                                 >  2:817054‑M2/140‑289 (MQ=255)
    agaagcgacaacgttaattcggacacccgccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgta                                                                                                                                                                                                                                                                              <  1:1300585‑M2/153‑1 (MQ=255)
       agcgacaacgttaattcggacacccgccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgtagt                                                                                                                                                                                                                                                                            <  1:419124‑M2/155‑1 (MQ=255)
                   aattcggccacccgccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCgg                                                                                                                                                                                                                                                              <  1:1000971‑M2/169‑1 (MQ=255)
                               gtccgggtttgagaacgacggttagaaatttcttcttaagcggctgctttccggtaactatgaaattacggaccttgatccggcgaatgacccgcgagccagttgattTCACTGAAAAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACtt                                                                                                                                                                                                                                                  <  2:1209038‑M2/181‑1 (MQ=255)
                               cgccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACtt                                                                                                                                                                                                                                                  <  2:1583420‑M2/181‑1 (MQ=255)
                                gccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACttt                                                                                                                                                                                                                                                 <  2:909931‑M2/182‑1 (MQ=255)
                                gccgggtttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACtt                                                                                                                                                                                                                                                  >  1:1709815‑M2/108‑288 (MQ=255)
                                      tttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCTCCGTTAATCACAAACACCAACCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCtt                                                                                                                                                                                                                                           >  1:571722‑M2/102‑289 (MQ=255)
                                      tttgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCt                                                                                                                                                                                                                                            <  2:1527347‑M2/187‑1 (MQ=255)
                                        tgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCGACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGGCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTAc                                                                                                                                                                                                                                         >  1:259006‑M2/100‑289 (MQ=255)
                                        tgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCCCAAACACCACCCCGCCGCCTCAGCCAAACTTACTACACGTACGTTCGGCAATTCTGCCTTTTCccttca                                                                                                                                                                                                                                         >  2:438824‑M2/100‑287 (MQ=255)
                                        tgatcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAACCCCAAACCCCACCCCCCCCCTTAAGCACAAGGAACAAAACTACCGTTCGGAAATTCTGCCTTTCCCcttt                                                                                                                                                                                                                                          >  2:1681738‑M2/100‑287 (MQ=255)
                                           tcacgacggttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAg                                                                                                                                                                                                                                       <  1:819509‑M2/192‑1 (MQ=255)
                                                   gttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCGTTACAGACATCCAg                                                                                                                                                                                                                               <  1:503342‑M2/200‑1 (MQ=255)
                                                    ttataaaatttttggtaagccgctgctttccggtaactatgaaattactgaactggatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAgg                                                                                                                                                                                                                              <  2:1330521‑M2/201‑1 (MQ=255)
                                                    ttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCaaa                                                                                                                                                                                                                                                                                  >  1:38242‑M2/88‑236 (MQ=255)
                                                    ttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACa                                                                                                                                                                                                                                        >  2:378733‑M2/88‑278 (MQ=255)
                                                    ttataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagatgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGc                                                                                                                                                                                                                             >  1:758451‑M2/88‑289 (MQ=255)
                                                      ataaaattctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCgt                                                                                                                                                                                                                           >  2:1786823‑M2/86‑289 (MQ=255)
                                                          aattctttgtaagccgctgctttccggtaactaggaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCa                                                                                                                                                                                                                       <  1:1810333‑M2/208‑1 (MQ=255)
                                                            ttctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACtt                                                                                                                                                                                                                                                  <  2:1867146‑M2/181‑1 (MQ=255)
                                                              ctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCatgat                                                                                                                                                                                                                   >  1:1951416‑M2/78‑289 (MQ=255)
                                                              ctttgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGCGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCatgat                                                                                                                                                                                                                   >  2:829185‑M2/78‑289 (MQ=255)
                                                                ttttactccgctcctttccggttactatgaaactactgaactttagccgacgaatgaccagcgagccagttgattTCACTAAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGTGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGATCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAg                                                                                                                                                                                                                                       <  1:1593794‑M2/192‑1 (MQ=255)
                                                                 tgtaagccgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTTAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACCTCCAAGCGTGTCATGATGtt                                                                                                                                                                                                                >  1:1639502‑M2/75‑289 (MQ=255)
                                                                    aagccgctgcgttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACTAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGa                                                                                                                                                                                                              <  2:932552‑M2/217‑1 (MQ=255)
                                                                        cgctgctttccggtaactatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAAt                                                                                                                                                                                                           <  1:115965‑M2/220‑1 (MQ=255)
                                                                                    gtaactatgaaattactgaacttgatccggcgaatgtccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAAACCTCCAGGCGTGTCATTATTTTGAAATCCATCGACATctg                                                                                                                                                                                              >  2:1034785‑M2/56‑288 (MQ=255)
                                                                                       actatgaaattactgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGGAGTAAACGTACGTTCGGCAATTCCGACTTTCGCCTTACAGACATCCAGGCGTGTTCTGATGTTGAAATCCATCGTCATcttccct                                                                                                                                                                                          >  2:648560‑M2/53‑289 (MQ=255)
                                                                                                   ctgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTc                                                                                                                                                                              <  1:555108‑M2/249‑1 (MQ=255)
                                                                                                    tgaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCg                                                                                                                                                                             <  1:986435‑M2/250‑1 (MQ=255)
                                                                                                     gaacttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGc                                                                                                                                                                            <  1:916080‑M2/251‑1 (MQ=255)
                                                                                                         ttgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCtttt                                                                                                                                                                        <  2:331584‑M2/255‑1 (MQ=255)
                                                                                                         ttgacccggcaaatgaccagggagccagttaattTCACTCAAAAACATTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCtttt                                                                                                                                                                        <  2:1273418‑M2/255‑1 (MQ=255)
                                                                                                         tgggtccggcgaatgaacaacgggccagttgattTCACGGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCtttt                                                                                                                                                                        <  2:195727‑M2/255‑1 (MQ=255)
                                                                                                          tgatccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTa                                                                                                                                                                       <  2:534299‑M2/256‑1 (MQ=255)
                                                                                                             tccggcgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTacc                                                                                                                                                                     >  2:1118679‑M2/31‑288 (MQ=255)
                                                                                                                  cgaatgaccagcgagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCGAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCAATCGACATCTGCCCTTCCGGAAGTTTCGCTTTTACCCACTgg                                                                                                                                                               >  2:1318484‑M2/26‑289 (MQ=255)
                                                                                                                              gagccagttgattTCACTGAGAAACATTTTACCTTGCGGTTGTAAAAGCCGCAGCGTGTGCCTGCCATCGACCCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCaa                                                                                                                                                     <  1:789364‑M2/274‑1 (MQ=255)
                                                                                                                              gagccagttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGCGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCa                                                                                                                                                      <  1:829185‑M2/273‑1 (MQ=255)
                                                                                                                                  cagttgattTCACTGAACAACGGTTTACCTTGCGGTTGTAAAAGACGGAGGGGGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCaa                                                                                                                                               <  2:1397080‑M2/280‑1 (MQ=255)
                                                                                                                                   agttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCtttt                                                                                                                                                                        <  2:711430‑M2/255‑1 (MQ=255)
                                                                                                                                   agttgattTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCtttt                                                                                                                                                                        >  1:711430‑M2/9‑263 (MQ=255)
                                                                                                                                            cACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCAGCAGGGTGTGCCTGCCAGCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGGGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGaa                                                                                                                                     <  2:443676/289‑1 (MQ=255)
                                                                                                                                               tGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACgg                                                                                                                                  >  1:1080914/1‑289 (MQ=255)
                                                                                                                                                 aacaacAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGATCCAAAGGTAGTACACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCCTGTCATGATGTTGCAATCCATCGACATCTGCCCTGCCCTCAGTTTCGCTTTTCCCACCTGGTCCGCTGCCAAGGCAAAAGGATGGAACGGtt                                                                                                                                >  2:1735920/1‑289 (MQ=255)
                                                                                                                                                    aacaGTTTACCTTGCGGTTGTAAAGGCCCCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCGCAGTTGCCATGCCCCGTGAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGGAAAGGCAAAAGGCTGGAACGGTTTTa                                                                                                                             <  1:1690154/289‑1 (MQ=255)
                                                                                                                                                      caGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCAAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAg                                                                                                                           >  1:935251/1‑289 (MQ=255)
                                                                                                                                                      caGTTTACCTTGCGGTTGTAAAAGCCGAAGGGTGTGCCTGCAATCGAACAAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCAGCCGCGTGAGCCAAAGGTCGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGGCATGATGTGGAATTCCATCAGCATCTGCCCTGCCATCAGTTTCGATTTTACCACCTGTCCCTCTGCAACGGCAAACAGCGAGAACGGATTTatg                                                                                                                           >  2:309773/1‑287 (MQ=255)
                                                                                                                                                        gTTTACCTGGCGTTGGTAAAGACGGCAGGGTTGCCCTGCCGACGACCCGGCCGGGGCCCGGGTCGGTAGTGGGCAATTTATCGCCCAGTTGCCCCGCCCCGTTCATCACAAACACCACCCCGCCGGGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGAGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTa                                                                                                                             <  2:1299936/285‑1 (MQ=255)
                                                                                                                                                         tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCCTGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCCTTTACCACCCGGTCCGCTGCCAAGGCCAAAGGCTGGACCGGGTTTAAGGTa                                                                                                                        >  2:1470961/1‑289 (MQ=255)
                                                                                                                                                         tttAACGTGGGGTTGAAAAAGCCGCCGGGTGTGCCGGCCATCGAACCACCACGGGGCCGGGTCGGTAGAGAGAAATTTATCGCCCAGTGGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGt                                                                                                                         <  2:1424545/288‑1 (MQ=255)
                                                                                                                                                          ttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGt                                                                                                                         <  1:245519/287‑1 (MQ=255)
                                                                                                                                                           tACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATg                                                                                                                      >  2:1653105/1‑289 (MQ=255)
                                                                                                                                                                tGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTTCCGTCAGTTTCGCTTTTACCACCTGGTCGGCTGCATATGCAAACGGCTGGAATGGTATTAAGGGATGGGTaa                                                                                                                 >  2:1546536/1‑289 (MQ=255)
                                                                                                                                                                tGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaa                                                                                                                 >  2:426260/1‑289 (MQ=255)
                                                                                                                                                                 gCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaaa                                                                                                                >  1:1003824/1‑289 (MQ=255)
                                                                                                                                                                 gCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaaa                                                                                                                >  1:975083/1‑289 (MQ=255)
                                                                                                                                                                 gCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCATGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaaa                                                                                                                <  2:228281/289‑1 (MQ=255)
                                                                                                                                                                  cGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaaa                                                                                                                >  1:1724712/1‑288 (MQ=255)
                                                                                                                                                                  cGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTAGGGTTAAAg                                                                                                               >  1:1040428/1‑289 (MQ=255)
                                                                                                                                                                           aaGCCTAAGGGTGCTCCTGTCATCGAACCAGCCATCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCCGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTgcg                                                                                                      <  2:571722/289‑1 (MQ=255)
                                                                                                                                                                                    ggTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAACAGGCTGGAACGGGTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAgg                                                                                             >  1:1569966/1‑289 (MQ=255)
                                                                                                                                                                                     ggttgcctgccCCCGAACCAGCACCGGCCCTGGTCGGTAGTGAGCCATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAgg                                                                                             <  2:975083/285‑1 (MQ=255)
                                                                                                                                                                                        tgcctgccATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTgcg                                                                                                      <  2:717884/276‑1 (MQ=255)
                                                                                                                                                                                              ccATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGACTGCGCTTTCAAGGAGCATCTcg                                                                                    >  1:850614/1‑288 (MQ=255)
                                                                                                                                                                                                    aaCCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCt                                                                              >  1:544653/1‑288 (MQ=255)
                                                                                                                                                                                                          cACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACCCCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGc                                                                        <  2:490270/288‑1 (MQ=255)
                                                                                                                                                                                                               gcCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGaa                                                                                                                                     <  1:372555/222‑1 (MQ=255)
                                                                                                                                                                                                                cccTGGTCGGTAGTGAGCAATTGATCGCCCAGTTGCCACGCCCCGTTAATCACAAGCACCACCCCGCCGCGTGAGCCAAAGGAAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCa                                                                 <  1:1858034/289‑1 (MQ=255)
                                                                                                                                                                                                                cccTGGTCGGTAGTGAGCAATTCATCGCCCAGCTGCCACGCCACGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTTAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTTCTACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAATGTATGGTTAAAGCGGTATGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGtta                                                                 <  1:1123714/289‑3 (MQ=255)
                                                                                                                                                                                                                 ccTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCGAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCa                                                                 <  1:1318484/288‑1 (MQ=255)
                                                                                                                                                                                                                 ccTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCa                                                                 <  1:1377710/288‑1 (MQ=255)
                                                                                                                                                                                                                  cTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAc                                                                >  1:1794560/1‑288 (MQ=255)
                                                                                                                                                                                                                    ggTCGGTAGTAAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTa                                                              >  2:271305/1‑288 (MQ=255)
                                                                                                                                                                                                                           aGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTc                                                                  >  1:1222386/1‑277 (MQ=255)
                                                                                                                                                                                                                           aGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTc                                                                  <  2:1222386/277‑1 (MQ=255)
                                                                                                                                                                                                                               aGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTg                                                                                                        >  1:465253/1‑235 (MQ=255)
                                                                                                                                                                                                                               aGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATg                                                   >  1:21257/1‑288 (MQ=255)
                                                                                                                                                                                                                                   acttACCGCCCCGTTGCCCCGCCCCGTTAATCCCAAACACCACCCCGCCGCGTGGGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCggg                                              <  2:1013948/287‑1 (MQ=255)
                                                                                                                                                                                                                                    tttATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGa                                             >  2:1794381/1‑289 (MQ=255)
                                                                                                                                                                                                                                    tttATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGCTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCCCGCCGCCTTACAGCAACGGCACTATCCTTTCCATGCCGGGa                                             >  2:867158/1‑289 (MQ=255)
                                                                                                                                                                                                                                      tATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCCCGCCGCCTTCCAGCAACCTCACTATCCTATCCATGTCGGGaaa                                           >  2:897009/1‑289 (MQ=255)
                                                                                                                                                                                                                                         cGCCCAGTTTCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCGGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGATTTCAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGACACTATCCTTTCCATGCCGGGcaacaa                                        >  1:1371938/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 tGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGaaa                                   <  1:1740891/286‑1 (MQ=255)
                                                                                                                                                                                                                                                          ccGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgccg                                                                                 <  2:684017/231‑1 (MQ=255)
                                                                                                                                                                                                                                                                aTCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCga                 <  1:1653105/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   acaAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatgg              >  1:154744/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     aaaCACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggat            >  1:328532/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     aaaCACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggat            >  2:1572734/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      aaCACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggatg           <  1:897009/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cgcccccccGCCCAGGCTGCCAAAGGTAGTAAACGTAGGTTCGTCAATTCGGACTTCCGGCCTACCGACATCCAGCCGGGGACTGATGTTGATATCCATCGACATCTGCCCTGCTTTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCGGGAACGGTTTTAAGGTATGGTTAAAGCAGTCTGCGCTTTCAACGAGCACCTCGCCGCCTTCCAGCACCGTCACTATCCTTGCCAGGCCGCGAAACAAAGAAAACTCCCCATTCGCCGCgatggatg           <  2:823105/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                         accaccCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCC‑TGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAAt       <  1:1870581/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           caccCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGaa                                                                                                                                     >  2:664100/1‑162 (MQ=255)
                                                                                                                                                                                                                                                                           caccCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGaa                                                                                                                                     <  1:664100/162‑1 (MQ=255)
                                                                                                                                                                                                                                                                           caccCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAAt       >  1:1176859/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                           caccCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAAt       >  1:195929/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                             ccccGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCt    >  2:1633623/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                      
GATCACGACGGTTATAAAATTCTTTGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTACTGAACTTGATCCGGGCTATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGACTGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATGCTCATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGG  >  CP042583/1506258‑1506817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A