Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,547,654 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,547,6540AT100.0% 602.2 / NA 255intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (120/135);  total (120/135)
*CP0425831,547,6550CG99.6% 602.1 / NA 255intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (119/135);  minor base T (1/0);  total (120/135)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,547,6560TA98.8% 596.4 / NA 255intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (118/134);  minor base C (2/1);  total (120/135)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGACTGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCC  >  CP042583/1547378‑1547932
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                    
      aTTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCCTGTTCCCCGCCCTTCCGCTGGCCGTGCCACCTCCCCACTAAAt                                                                                                                                                                                                                                                                      >  2:1628666/1‑289 (MQ=255)
          cgaCGGCGTAAACTGGCTTGGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGa                                                                                                                                                                                                                                                                   <  2:1091057/287‑1 (MQ=255)
          agaCGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGaa                                                                                                                                                                                                                                                                  >  1:122410/1‑289 (MQ=255)
               gCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCAtt                                                                                                                                                                                                                                                             >  1:866442/1‑289 (MQ=255)
               gCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCAtt                                                                                                                                                                                                                                                             >  2:1706264/1‑289 (MQ=255)
                    aaCTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTttg                                                                                                                                                                                                                                                        <  1:1480593/289‑1 (MQ=255)
                         gCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTttgtt                                                                                                                                                                                                                                                      <  1:267200/286‑1 (MQ=255)
                          cTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCa                                                                                                                                                                                                                                                   <  1:1774238/288‑1 (MQ=255)
                          cTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCAt                                                                                                                                                                                                                                                  >  2:1907635/1‑289 (MQ=255)
                          cTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCCTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCACTGAGGGTTAAACCGAGTTCAATGCCAGCGCGTAGTACAGCAACTTCTGGTTTGCGGT‑GACTGGCATCTTCGCCCATCTACCATGTTCCCTGCCCTACGGCCGGCAGTTAGACCTGCCCCCTAAATATAATCGTTTTTTTTTGCAt                                                                                                                                                                                                                                                  >  2:1395282/1‑289 (MQ=255)
                              gcatgcatTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGtt                                                                                                                                                                                                                                              <  2:726178/289‑1 (MQ=255)
                               catgcatTTAACGCTTTCTGCCCACCAGCATTCCACGGATAGGCTTTAGAGACTAGAAAGAACTACTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTGTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAAGGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGtt                                                                                                                                                                                                                                              <  2:1725481/288‑1 (MQ=255)
                                     tttATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTccc                                                                                                                                                                                                                                       <  2:1501907/289‑1 (MQ=255)
                                     attATCGCTTTTTTCCCGCCAGCATTCCACGGTTCGACTTTAGAGACTGGTAAGACCTCCTCTCCCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACGTTTCGGCGGTATCAATGTGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTccc                                                                                                                                                                                                                                       <  2:504539/288‑1 (MQ=255)
                                      ttATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCa                                                                                                                                                                                                                                      <  1:1706264/289‑1 (MQ=255)
                                              ttttGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGc                                                                                                                                                                                                                              >  1:426479/1‑289 (MQ=255)
                                                ttGCCCGCCAGCATTCGCCGGATAGACTTTAGAGACGAGCAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGcac                                                                                                                                                                                                                            <  2:332973/289‑1 (MQ=255)
                                                     cGCCAGCATTCCACGGATAGACTTTAGAGACGCGAAAGACCTCCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTGCTGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCg                                                                                                                                                                                                                       <  1:1093097/289‑1 (MQ=255)
                                                            aTTCCACGCTTATACTTTAGAGACTAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAGCCACCTTTTCGGCTCCGCCATCGGCATACATGTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATg                                                                                                                                                                                                                  <  2:1333141/287‑1 (MQ=255)
                                                               ccACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCaaaa                                                                                                                                                                                                             <  2:1589364/289‑1 (MQ=255)
                                                                          cTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTTCCTGCCCTACGGCTGGCTGTGAGACATCGCCACTAAATTGAATCATTTTTTTTTTCCTTGTTTCCCCCCAGGTATTTCACCACCGTAATGCAAAACAGGCCGTgt                                                                                                                                                                                                   >  2:471133/1‑287 (MQ=255)
                                                                             tAGAGTCGAGAAAGCCCTTCTCTCGCAGACCGGTTAATGCTGCCCCAACCCCCTTTTCGGCAACGCCATCGGCATACATTTCGGCGGGATCAATGAGGGTTAAACCGAGTTCAATGCCCGCTCGTAGTGCAGCGACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                                                                                                                               <  2:303564/289‑1 (MQ=255)
                                                                             tAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGc                                                                                                                                                                                                >  2:1558305/1‑288 (MQ=255)
                                                                             tAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTGTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                                                                                                                               <  1:698530/289‑1 (MQ=255)
                                                                              agagcctctaaaGACCTTCCCTCGGCGACCGGTTAATGCTTCCCCCACCACCGTTTCGGGACGGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                                                                                                                              <  2:82365/280‑1 (MQ=255)
                                                                                     agaAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCg                                                                                                                                                                                                                       <  2:1226877/257‑1 (MQ=255)
                                                                                     agaAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttttt                                                                                                                                                                                       >  1:765576/1‑289 (MQ=255)
                                                                                         aGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGc                                                                                                                                                                                   >  1:630331/1‑289 (MQ=255)
                                                                                              ttcccccGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                                                                              <  1:59561/283‑1 (MQ=255)
                                                                                              ttCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                                                                              <  1:11924/289‑1 (MQ=255)
                                                                                              ttCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                                                                              >  1:1527097/1‑289 (MQ=255)
                                                                                              ttCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGCTTTTATGCACaaa                                                                                                                                                                              >  1:1900267/1‑289 (MQ=255)
                                                                                               tctctcGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                                                                                                                             <  1:1067732/289‑1 (MQ=255)
                                                                                               tctctcGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCAATAAATTGAACCATTTTTTGTTGCATTGTTTCCTTCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTTACTCCCTGATTTTATGCACcaaa                                                                                                                                                                             >  2:1739817/1‑289 (MQ=255)
                                                                                                ctctcGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCCGTGAGACATCGCCACTACATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCTTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAACAt                                                                                                                                                                            >  2:409193/1‑289 (MQ=255)
                                                                                                 tctcGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATg                                                                                                                                                                           <  1:15835/289‑1 (MQ=255)
                                                                                                      cAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCa                                                                                                                                                                                                                                                   <  2:201259/212‑1 (MQ=255)
                                                                                                      cAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTg                                                                                                                                                                      >  1:1245327/1‑289 (MQ=255)
                                                                                                      cAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTg                                                                                                                                                                      >  1:405418/1‑289 (MQ=255)
                                                                                                          ccGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTTCACCACCGTAATGCAAAACAGGGCGTACCGCCCTGTCTTTATGCACAAAATGCCCTGGaaa                                                                                                                                                                  >  2:1849758/1‑289 (MQ=255)
                                                                                                            ggTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAATCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                                                                                                                <  1:1146330/289‑1 (MQ=255)
                                                                                                             gTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCCTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACCCCCTGTTTTTATTCACCAAATGCCCCGGAAAGAt                                                                                                                                                               >  2:1207458/1‑289 (MQ=255)
                                                                                                             gTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                                                               >  2:541258/1‑289 (MQ=255)
                                                                                                             gTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                                                               >  1:369743/1‑289 (MQ=255)
                                                                                                                   gCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAgg                                                                                                                                                                                                                                    <  1:1080266/214‑1 (MQ=255)
                                                                                                                       ccccAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCCATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTCGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGTTATTGCACCACCGTAATGCAAAACAGGGCGTTACTCCCTGCTTTTATGCACAAACTGCCCTGGAAAGATGCATAATCAg                                                                                                                                                     >  2:1577922/1‑289 (MQ=255)
                                                                                                                       ccccAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTTTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTTACGCCCTGTTTTTATGCACAAAATGACCTGGAAAGATGCATTagtag                                                                                                                                                     >  2:984342/1‑285 (MQ=255)
                                                                                                                       ccccAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                                                                                                                     >  2:781019/1‑289 (MQ=255)
                                                                                                                        cccATCCACCTGTTCGGCACCGCCATCGGCATACATTTCCGCGGTATCAATGCGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGa                                                                                                                                                    <  2:1002354/289‑1 (MQ=255)
                                                                                                                        cccAACCCCCTGTTCGGCACCGCCATCGGCATACCTTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGa                                                                                                                                                    <  2:1657890/289‑1 (MQ=255)
                                                                                                                                                      aTACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCCACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                      <  1:884098/289‑1 (MQ=255)
                                                                                                                                                      aTACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                                                                         >  2:799977/1‑238 (MQ=255)
                                                                                                                                                      aTACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                                                                         <  1:799977/238‑1 (MQ=255)
                                                                                                                                                      aTACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                      <  1:1683980/289‑1 (MQ=255)
                                                                                                                                                      aTACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                      <  1:1443645/289‑1 (MQ=255)
                                                                                                                                                      aTACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATACCGGTAGAAATCAGGc                                                                                                                      <  1:1849758/289‑1 (MQ=255)
                                                                                                                                                      aTACAGTTCGGCGGTATAAATGTGGGTTAAACCGAGTTCAATGCCCGCGCGTAGGGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                      <  2:426479/289‑1 (MQ=255)
                                                                                                                                                        aCATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCa                                                                                                                    >  1:235145/1‑289 (MQ=255)
                                                                                                                                                               ggcggTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg                                                                                                             >  1:1869403/1‑289 (MQ=255)
                                                                                                                                                               ggcggTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGG‑AAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                                                                            >  2:112413/1‑289 (MQ=255)
                                                                                                                                                                 cggTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt                                                                                                           >  1:1924101/1‑289 (MQ=255)
                                                                                                                                                                  ggTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt                                                                                                           <  1:857768/288‑1 (MQ=255)
                                                                                                                                                                              ggTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCCCCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATCCCCTGGAAAAATGCATTATCAGAATTGGTAGGTGATCCCGGTAGAAATTAGGCAAGTCCATGATTTTTCCACCATCg                                                                                              >  2:1146228/1‑289 (MQ=255)
                                                                                                                                                                                 tAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCggg                                                                                            <  2:37237/288‑1 (MQ=255)
                                                                                                                                                                                        aGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                                                                                    >  1:1623620/1‑289 (MQ=255)
                                                                                                                                                                                         gTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAgg                                                                                                                                                                                                                                    >  2:570310/1‑144 (MQ=255)
                                                                                                                                                                                          ttCAATGCCCGCGCGTAGTGAAGCAACTTCTGTTTTGCGCT‑TACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                                                                  <  1:743740/289‑1 (MQ=255)
                                                                                                                                                                                           tCAATGCCCGAGCCTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTTGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGAACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                                                                  <  2:1691310/288‑1 (MQ=255)
                                                                                                                                                                                               tGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtca                                                                             >  1:1598963/1‑289 (MQ=255)
                                                                                                                                                                                                      gcgTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagaca                                                                      <  1:350167/289‑1 (MQ=255)
                                                                                                                                                                                                        gTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                                                                    <  1:1558305/289‑1 (MQ=255)
                                                                                                                                                                                                             gcagcaACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACgg                                                                 <  1:12644/287‑1 (MQ=255)
                                                                                                                                                                                                              cagcaACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                                                                                                                         <  1:1074324/166‑1 (MQ=255)
                                                                                                                                                                                                              cagcaACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                                                                                                                         >  2:1074324/1‑166 (MQ=255)
                                                                                                                                                                                                                gcaACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCAGCCGACAGCCGGGTGTa                                                            >  2:906896/1‑289 (MQ=255)
                                                                                                                                                                                                                    cTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGctt                                                        >  1:1038627/1‑287 (MQ=255)
                                                                                                                                                                                                                     ttCTTTTTTGCGCT‑GCATGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg                                                       <  1:310615/289‑1 (MQ=255)
                                                                                                                                                                                                                     ttCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg                                                       <  2:235145/289‑1 (MQ=255)
                                                                                                                                                                                                                     ttCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg                                                       <  2:1527097/289‑1 (MQ=255)
                                                                                                                                                                                                                     ttCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg                                                       <  2:860637/289‑1 (MQ=255)
                                                                                                                                                                                                                      tCTGTTTTGCGCT‑GTCTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc                                                      >  1:1674607/1‑289 (MQ=255)
                                                                                                                                                                                                                      tCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc                                                      >  2:360341/1‑289 (MQ=255)
                                                                                                                                                                                                                      tCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc                                                      >  2:949341/1‑289 (MQ=255)
                                                                                                                                                                                                                      tCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc                                                      >  2:667826/1‑289 (MQ=255)
                                                                                                                                                                                                                       cTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTGAGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCg                                                     <  2:1434104/289‑1 (MQ=255)
                                                                                                                                                                                                                       cTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCg                                                     <  2:1038627/289‑1 (MQ=255)
                                                                                                                                                                                                                       cTGTTTTGCGCT‑GACTGGCATCTGCGCGCATAGACCAGGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTGTCCTCACAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCg                                                     <  2:532368/289‑1 (MQ=255)
                                                                                                                                                                                                                        ctgtttGCGCCGGACTGTGATCTTCGCCAATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCg                                                     <  2:630331/286‑1 (MQ=255)
                                                                                                                                                                                                                                          cATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc                                                <  1:565246/276‑1 (MQ=255)
                                                                                                                                                                                                                                           aTCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGt                                  <  2:31270/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  cccATATTCCATGTTCCCTACCATATGGCTGGCAGTGCGGCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTACTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAg                           <  2:1272689/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             tGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGt                                                   <  2:1076660/254‑1 (MQ=255)
                                                                                                                                                                                                                                                             tGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAg                 >  2:1889419/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             tGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGc                >  1:1005017/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               ttCCCTGCCCTCCGGCTGGCAGTGATACATAGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTg              <  2:860314/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCTAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCt         <  1:1786473/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGt                                                                                                                                 >  2:655624/1‑168 (MQ=255)
                                                                                                                                                                                                                                                                     gCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTc        <  2:765576/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTcc  >  1:467400/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTcc  >  1:1372164/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                    
ATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCT‑GACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGACTGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCC  >  CP042583/1547378‑1547932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A