Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,662,183 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,662,1830GC99.6% 570.5 / ‑7.1 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (138/105);  minor base T (0/1);  total (138/106)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,662,1840CG99.2% 565.7 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (138/102);  total (138/106)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,662,1850TA99.2% 573.8 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (137/104);  minor base C (1/1);  total (138/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTAGTAGCCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGGCTAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCG  >  CP042583/1661898‑1662462
                                                                                                                                                                                                                                                                                             |||                                                                                                                                                                                                                                                                                     
     cAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAg                                                                                                                                                                                                                                                                                 <  1:1666478/289‑1 (MQ=255)
      aaaTCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAgg                                                                                                                                                                                                                                                                                <  1:1037470/289‑1 (MQ=255)
            ctGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACAtt                                                                                                                                                                                                                                                                          <  1:546569/289‑1 (MQ=255)
                         tatCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTaa                                                                                                                                                                                                                                                             >  2:837082/1‑289 (MQ=255)
                               aGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcc                                                                                                                                                                                                                                                        >  1:1709350/1‑288 (MQ=255)
                                            tcatggcaaaaaactAAAATTAAACCTAAAATCAAAAAGCCATATAAACAGGTTATCTACCATGTCTTCCGCATGACGGATAATGTGACTAATGGTATTTTTGGAAACATGCGCTAACACATTCTGACTGGTGGTCTCCCACCAGTCATGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCTGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTAc                                                                                                                                                                                                                                          <  2:1058206/275‑1 (MQ=255)
                                            tcatcgcATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTAc                                                                                                                                                                                                                                          <  1:578494/283‑1 (MQ=255)
                                            tcatcgcATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTAc                                                                                                                                                                                                                                          <  1:837082/283‑1 (MQ=255)
                                            gcctcccATATAGGTAATATTAAACGTAAGATCACAACACATAATGAACAAGTTATCTACCATGTCATCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTTGTGGTTTCCCACCAGTCAGGCTGAATACGATTACTCTGCTTTCTCCACAAAGATATCGTCCTGATGCCCTGCTTCATTAACGATCCAGATACCGAGCGAGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTCCCAGTag                                                                                                                                                                                                                                          <  2:722349/284‑2 (MQ=255)
                                                  cATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTCCCTTCACTAAACTCCCCAACTGCGAGTACCACGc                                                                                                                                                                                                                                     >  2:1067315/1‑288 (MQ=255)
                                                                   aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATTCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCTACAAAGATACCGTCCTGATGCCCTGCTTCTTTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCCGCGAAACCAGGTAAATTGTGCCTTCACTAAACTCCTCACCTGCCAGTACCACGCCAGGACCAGCCGGACCg                                                                                                                                                                                                                    >  2:1579906/1‑288 (MQ=255)
                                                                   aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGACGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCg                                                                                                                                                                                                                    >  2:247676/1‑288 (MQ=255)
                                                                   aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCCGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGc                                                                                                                                                                                                                   <  1:161225/289‑1 (MQ=255)
                                                                       aaGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACAAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCAt                                                                                                                                                                                                                >  1:1337694/1‑288 (MQ=255)
                                                                         gATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCg                                                                                                                                                                                                             >  1:1436755/1‑289 (MQ=255)
                                                                               aaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGa                                                                                                                                                                                                       <  1:799502/289‑1 (MQ=255)
                                                                               aaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCACTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTg                                                                                                                                                                                                        >  2:228893/1‑288 (MQ=255)
                                                                                    ataataAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTTACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCTCCAAGATCCCGTCCTTATGCCATGCTTCATTAACGACCTCGACGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACAGTGTGCCTTCACTAAACTCCTCAACTCCCAGTACCACGACCGGACACCGCGGACCGCCATCCGTGTTGACTGtg                                                                                                                                                                                                  >  2:590798/1‑288 (MQ=255)
                                                                                    ataataAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGt                                                                                                                                                                                                   <  1:969006/288‑1 (MQ=255)
                                                                                       ataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTAcc                                                                                                                                                                                               <  1:342814/289‑1 (MQ=255)
                                                                                              ggTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCg                                                                                                                                                                                             >  2:1835933/1‑284 (MQ=255)
                                                                                                ttATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTc                                                                                                                                                                                      >  2:319142/1‑289 (MQ=255)
                                                                                                  aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTAccc                                                                                                                                                                                              <  2:916428/279‑1 (MQ=255)
                                                                                                  aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCa                                                                                                                                                                                     >  2:1629496/1‑288 (MQ=255)
                                                                                                  aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCa                                                                                                                                                                                     >  1:446209/1‑288 (MQ=255)
                                                                                                  aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCa                                                                                                                                                                                     >  1:1882528/1‑288 (MQ=255)
                                                                                                        cATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCAt                                                                                                                                                                              <  1:147395/289‑1 (MQ=255)
                                                                                                         aTGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTTCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTTATTAAAGAACCAGATGCCGAACGGGTAGTCTTCCAGCGAAACAAGGTACATTGTGCCTTCAATAAACTCCTCAACTTTCAGTACCACGCACGGAAGACGCCGCTCGCACTCCGTTTTCACTGTTACCCGa                                                                                                                                                                                            >  2:950096/1‑274 (MQ=255)
                                                                                                         aTGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGAGGGTAGTCTTCTAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGCCGCGGACCGCCATCCGATTTGACTTGTACCCGATCATTCACCTTCATc                                                                                                                                                                             >  2:658028/1‑289 (MQ=255)
                                                                                                          tGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCg                                                                                                                                                                            >  1:1430696/1‑289 (MQ=255)
                                                                                                             cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTAccc                                                                                                                                                                                              >  1:597378/1‑268 (MQ=255)
                                                                                                              gtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTTCCTTTTACCCGACCATTCACCTTCAACGgtt                                                                                                                                                                         >  2:493897/1‑288 (MQ=255)
                                                                                                              gtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGtttt                                                                                                                                                                        <  1:11329/289‑1 (MQ=255)
                                                                                                               tcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAATAACCAGATGCCGAGCGGGTAGTCTTCCCGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCCATACCATGCACGCAAGACGCGGACCGCCATACTTTTTTGAGGTTACCCGGTCATTCCCCTTCATCGgttca                                                                                                                                                                       >  2:1494580/1‑287 (MQ=255)
                                                                                                               tcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAgg                                                                                                                                                                                                                                                                                >  1:770846/1‑184 (MQ=255)
                                                                                                               tcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAgg                                                                                                                                                                                                                                                                                <  2:770846/184‑1 (MQ=255)
                                                                                                                 cGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGGTTTGACTGTTACCCGATCATTCACCTTCATCGTTTtcc                                                                                                                                                                      >  2:719637/1‑288 (MQ=255)
                                                                                                                     tGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCtg                                                                                                                                                                 <  2:1709350/289‑1 (MQ=255)
                                                                                                                      gACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCtgt                                                                                                                                                                >  2:78460/1‑289 (MQ=255)
                                                                                                                        cGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTgg                                                                                                                                                              <  2:967381/289‑1 (MQ=255)
                                                                                                                          gATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCt                                                                                                                                                            >  2:31300/1‑289 (MQ=255)
                                                                                                                                gtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGc                                                                                                                                                      <  2:1932493/289‑1 (MQ=255)
                                                                                                                                ggtaCTAATTGTATTTTTGGCAACCTGCGCTAACACATTCGGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGc                                                                                                                                                      <  2:400762/286‑1 (MQ=255)
                                                                                                                                     tAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAgtg                                                                                                                                                 >  2:1528867/1‑289 (MQ=255)
                                                                                                                                         ggTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGa                                                                                                                                             >  1:298726/1‑289 (MQ=255)
                                                                                                                                                       cATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTTTGCCAGTGTAGAAAAATTTCTtttt                                                                                                                                >  2:1805577/1‑288 (MQ=255)
                                                                                                                                                          gcgcTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTg                                                                                                                            <  2:1249489/289‑1 (MQ=255)
                                                                                                                                                          gcgcTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTg                                                                                                                            >  1:1363924/1‑289 (MQ=255)
                                                                                                                                                            gcTAACACGTTCTGACTGGTCGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCCCAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGc                                                                                                                          <  2:599323/289‑1 (MQ=255)
                                                                                                                                                            gcTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGc                                                                                                                          <  2:446209/289‑1 (MQ=255)
                                                                                                                                                                      tCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGTCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTAACCTTCATCGTTTTCCTCCTGTGGCCTTGTGCCAGTGTAGAACAATTTCGTTTTTCTTGCAGCCCCCgg                                                                                                                 >  2:216647/1‑288 (MQ=255)
                                                                                                                                                                        tGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCa                                                                                                                                                                                                                                                     >  1:899176/1‑154 (MQ=255)
                                                                                                                                                                          aCTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgcgc                                                                                                            >  1:925515/1‑289 (MQ=255)
                                                                                                                                                                          aCTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGgcgcgc                                                                                                            >  1:340044/1‑289 (MQ=255)
                                                                                                                                                                              gtggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGCCGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCCGGGCCCCGCGa                                                                                                        >  2:1694307/1‑289 (MQ=255)
                                                                                                                                                                              gtggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGa                                                                                                        >  1:220061/1‑289 (MQ=255)
                                                                                                                                                                                              cAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCg                                                                                         <  1:942360/288‑1 (MQ=255)
                                                                                                                                                                                              cAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCg                                                                                         <  1:714296/288‑1 (MQ=255)
                                                                                                                                                                                              cAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa                                                                                        <  2:1861274/289‑1 (MQ=255)
                                                                                                                                                                                              cAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa                                                                                        <  1:78460/289‑1 (MQ=255)
                                                                                                                                                                                              cAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa                                                                                        <  2:1436755/289‑1 (MQ=255)
                                                                                                                                                                                                   tGAATAAGCTTACTCTGCTTTCTCCACAACGAGACCGTCCTGATGCCCTGCTTCATTAAAGATCCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGt                                                                                    <  2:1367340/288‑1 (MQ=255)
                                                                                                                                                                                                   tGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGt                                                                                    <  1:247676/288‑1 (MQ=255)
                                                                                                                                                                                                     gataataTTATGCTGATTTGTCCACGAAGATGCAGTCCTTATGCCCCGCTTCAATAAAGGACAAGTTGTCATGAGGGTAGTCTTCCAGCTCAACCAGGTACTTTTTGCCTTCACTAAGCTCCTCAACTGACAGTACCACGCCAGGACGACGCGGAACGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTTGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCta                                                                                 <  2:1003486/288‑1 (MQ=255)
                                                                                                                                                                                                      aTAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCta                                                                                 <  2:1363924/288‑1 (MQ=255)
                                                                                                                                                                                                      aTAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCta                                                                                 <  1:1845484/288‑1 (MQ=255)
                                                                                                                                                                                                       tAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGAt                                                                                                                                                                                           <  1:1002020/181‑1 (MQ=255)
                                                                                                                                                                                                           aTTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGt                                                                                    <  2:66824/280‑1 (MQ=255)
                                                                                                                                                                                                           aTTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGt                                                                                    >  1:66824/1‑280 (MQ=255)
                                                                                                                                                                                                               ctctGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAagag                                                                       >  1:1631583/1‑289 (MQ=255)
                                                                                                                                                                                                                    cTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCTCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGcc                                                                  >  1:1171354/1‑289 (MQ=255)
                                                                                                                                                                                                                    cTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCg                                                                                         <  2:1236570/266‑1 (MQ=255)
                                                                                                                                                                                                                    cTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCg                                                                                         >  1:1236570/1‑266 (MQ=255)
                                                                                                                                                                                                                    cTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGcc                                                                  >  1:1523258/1‑289 (MQ=255)
                                                                                                                                                                                                                    cTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGcc                                                                  >  1:590350/1‑289 (MQ=255)
                                                                                                                                                                                                                       tctcCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAg                                                                >  1:4940/1‑288 (MQ=255)
                                                                                                                                                                                                                         tcCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTTTGGCTTTGTTCCAGTGTATAACAATTTCGTTTTTCTGGCTGCCCCGGGCGCGCGCGAGTGCTGATTTTCTCGATGTGTTATACTTTACAGATgactggtg                                                             >  2:524625/1‑282 (MQ=255)
                                                                                                                                                                                                                         tcCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCg                                                             <  1:180463/289‑1 (MQ=255)
                                                                                                                                                                                                                         tcCACAAAGATACCGTCCTGATGCCCTGCTTCAGTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCg                                                             <  1:1059599/289‑1 (MQ=255)
                                                                                                                                                                                                                                       gTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAgg                                                               >  1:1558542/1‑273 (MQ=255)
                                                                                                                                                                                                                                        tCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTTGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTggc                                              >  2:695574/1‑289 (MQ=255)
                                                                                                                                                                                                                                        tCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTTGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGAATgg                                               >  2:650767/1‑288 (MQ=255)
                                                                                                                                                                                                                                        tCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTggc                                              <  2:849914/289‑1 (MQ=255)
                                                                                                                                                                                                                                            gATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCCGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTGAGACATGCAAGGCGGACTTAACGACTGGCGGca                                          >  2:757780/1‑289 (MQ=255)
                                                                                                                                                                                                                                            gATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa                                                                                        >  2:1115800/1‑243 (MQ=255)
                                                                                                                                                                                                                                            gATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGca                                          >  1:420404/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 ccTGCTTCATTAAAGAACCAGCTGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGcaacaa                                      <  1:962259/288‑1 (MQ=255)
                                                                                                                                                                                                                                                 ccTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGcaacaa                                      <  1:1285149/288‑1 (MQ=255)
                                                                                                                                                                                                                                                  cTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCt                                                                                  >  2:939773/1‑243 (MQ=255)
                                                                                                                                                                                                                                                   tGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACag                                   >  2:511438/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   tGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACag                                   <  1:651030/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     cTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACagag                                 >  1:20366/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      ttCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGt                                <  1:1629496/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             aaGGACCCGAAGCCGGGCGTGTCGTCTGCCAGCCAAACCAGGTAAATTGTGCCTGCACTAAACCCCGCCACTGCCAGTACCAAGCCAGGACGACGCGGACCGCCATCCGTTGTGACTGTTACCCGATCATTCACCGTCAGCGTGTGCCTCCTGTGGCTTTGAGACAGTGTAGAACAATTGCGTTTCGCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCGCGACGGTCTAAACTTAAGAGAGGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGtt                         <  2:1189320/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               gAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa                                                                                        >  2:1021505/1‑224 (MQ=255)
                                                                                                                                                                                                                                                               gAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGa                                                                                        <  1:1021505/224‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aCCAGATGCCAAGCGGGTAGTCTTCCAGCTAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGc                       <  1:1388413/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gggTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGaa       <  1:511438/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ggTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAATAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGACGATGaaa      >  1:1078470/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      cTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCg  <  1:319142/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             |||                                                                                                                                                                                                                                                                                     
CCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTAGTAGCCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGGCTAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAGTACCACGCCAGGACGACGCGGACCGCCATCCGTTTTGACTGTTACCCGATCATTCACCTTCATCGTTTTCCTCCTGTGGCTTTGTGCCAGTGTAGAACAATTTCGTTTTTCTGGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCG  >  CP042583/1661898‑1662462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A