Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,663,584 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,663,5840AT98.4% 571.5 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (112/128);  minor base G (0/4);  total (112/132)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,663,5850GC99.6% 573.3 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (110/132);  total (112/132)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,663,5860TA100.0% 578.5 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (112/132);  total (112/132)

Only 100 of 244 total aligned reads displayed.

GAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGAGCGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGAGTGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGTCGGCCAG  >  CP042583/1663304‑1663861
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                    
gaaagaaCATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCATCTGCCGGCAAAGATCAGACTGCTAACTGGATTATCGCGACCTATCAACAAGTGTCAGGCaaa                                                                                                                                                                                                                                                                                >  1:1376721/1‑289 (MQ=255)
  aagaaCATTTTAAATTCACGACTTTTGATGATGTCGACGATGAAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACg                                                                                                                                                                                                                                                                              <  2:1128557/289‑1 (MQ=255)
     aaCATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGccc                                                                                                                                                                                                                                                                           >  1:997718/1‑289 (MQ=255)
      caaTTTTAAATTCACGCAGTTGTAGGATGTCGACTATGCAACCATGGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGcc                                                                                                                                                                                                                                                                            <  2:190789/285‑1 (MQ=255)
        aTTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAc                                                                                                                                                                                                                                                                        <  1:1249694/289‑1 (MQ=255)
          tttAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCCCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAc                                                                                                                                                                                                                                                                        >  2:1109606/1‑287 (MQ=255)
            tAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATATGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAcccc                                                                                                                                                                                                                                                                     >  1:1545685/1‑286 (MQ=255)
            tAAAGTCACGGCTTTTGATGATGTAGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACcaca                                                                                                                                                                                                                                                                    <  2:257516/289‑1 (MQ=255)
             aaaTTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCacac                                                                                                                                                                                                                                                                   <  1:1064797/289‑1 (MQ=255)
              aaTTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACt                                                                                                                                                                                                                                                                  <  1:32336/289‑1 (MQ=255)
               aTTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGCACTGGATGCTTCTGCCGGAAAAGTTCAGGCTGCCAACTGGATTATCGCGACCTATCTACAATTGTCAGGCAAAACCACAACCACACtt                                                                                                                                                                                                                                                                 >  2:1359745/1‑289 (MQ=255)
                         tttGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCCCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCg                                                                                                                                                                                                                                                       <  1:1309106/289‑1 (MQ=255)
                          ttGATGATGTCGGAGATGCAACCATCGCCGAATGGACGGGATTAAGCCGGAGCCAGGGGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGa                                                                                                                                                                                                                                                      <  2:1007636/289‑1 (MQ=255)
                                 gggcgaggaGGCAACCAGGGCCGAATGGACGGGTTTAAGCCCGAGCCGGGCGGCGCTGACGAAGCTTCATGGGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGcc                                                                                                                                                                                                                                                <  2:1236606/285‑1 (MQ=255)
                                   cagacaaggacaCCCTCGCCGAATGAGCGGGATGAAGCCGTAGCCAGCCGGCGCGGACGCAGCTTCATGGGGCGTCGGT‑AACGCGAATCTGGCGCGACTGTGACGGGCGTATGGCACAATTTACCGCGCGTCTGAACGAACTGTGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCa                                                                                                                                                                                                                                               <  2:1545685/276‑1 (MQ=255)
                                        gaTGCAACCCACGCGGAATGGACGGGATTAGGCCGCAGCCAGGCGGCGCTGCGGCCGCTGCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGGGCGTATGGCACAATTTACCGCTCGTCTGAACGAGCTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACGGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGGTg                                                                                                                                                                                                                                        <  2:804724/289‑1 (MQ=255)
                                        gaTGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATg                                                                                                                                                                                                                                        <  1:305667/289‑1 (MQ=255)
                                          tgccacaatggacGAAGGGACGGGGTTAAGCCGGAGCCAGGGGGCGCTGCCGCAGCGTCATGAGGCG‑TCGTGAACGCTAATCTGGCGCGACAGTGACGGGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATgc                                                                                                                                                                                                                                       <  2:1598572/276‑1 (MQ=255)
                                          tGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATgc                                                                                                                                                                                                                                       <  2:1790432/288‑1 (MQ=255)
                                          tGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATgc                                                                                                                                                                                                                                       <  1:1397988/288‑1 (MQ=255)
                                          tGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATgc                                                                                                                                                                                                                                       <  1:610092/288‑1 (MQ=255)
                                                    gCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATgc                                                                                                                                                                                                                                       >  1:502360/1‑278 (MQ=255)
                                                             aCGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAAGTGGAATATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCATGGGCTCTGGGCAAACACATGCGCTCTTACTGGAGGTGATgg                                                                                                                                                                                                                   >  2:414717/1‑289 (MQ=255)
                                                                  aTTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGCCAGTGACGAGCGTATGGCACACTTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTGCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTAAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGACCCCTTACTGGAGGTAAGGGCTTa                                                                                                                                                                                                               >  2:1400389/1‑288 (MQ=255)
                                                                     taGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGGTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCATGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgcg                                                                                                                                                                                                           <  1:1677813/288‑1 (MQ=255)
                                                                     aaGCCGTAGCCAGGCGGCGCTGAGGCGGGTTCATGAGGCGTCGGT‑AACGCTAATCGGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgcg                                                                                                                                                                                                           <  2:1836415/289‑1 (MQ=255)
                                                                     aaGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgcg                                                                                                                                                                                                           <  2:1758691/289‑1 (MQ=255)
                                                                     aaGCCGGAGCCGGGCGGGGCAGACGGAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgcg                                                                                                                                                                                                           <  2:985765/289‑1 (MQ=255)
                                                                           tAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGAtt                                                                                                                                                                                                     >  1:908030/1‑289 (MQ=255)
                                                                               cggggggggcTGGCGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGa                                                                                                                                                                                                 <  2:1471050/281‑1 (MQ=255)
                                                                               cAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGACACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGa                                                                                                                                                                                                 <  1:144341/289‑1 (MQ=255)
                                                                                  gcggcgCTGACGCAGCTTCATGAGGCGTCGGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAg                                                                                                                                                                                              >  1:1471050/1‑289 (MQ=255)
                                                                                                    atagggggctcggT‑AACGCCAATAGGGCGCGAAAGTGCCGAGCGTATGGCACAATTTACCGCTCGTGTGAACGAACTGGGCTTACCGTTTATGCAGGGGGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAgggg                                                                                                                                                                            <  2:548761/277‑1 (MQ=255)
                                                                                                          gcgtcggT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTTCCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCAt                                                                                                                                                                       <  1:700305/282‑1 (MQ=255)
                                                                                                           cgtcggT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCa                                                                                                                                                                        >  1:67361/6‑286 (MQ=255)
                                                                                                             tcggT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCg                                                                                                                                                                                                                                                       >  2:315562/4‑205 (MQ=255)
                                                                                                             tcggT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGc                                                                                                                                                                   >  1:1165582/4‑289 (MQ=255)
                                                                                                             tcggT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGc                                                                                                                                                                   >  2:935945/4‑289 (MQ=255)
                                                                                                                gggaaCGCTAATCTGGCGGGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTATGAACGAACTGGGCTTACAGTTTATGCAGGGTTCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCCACAATTGTCAGGCAAACGCCCAACCCCACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCat                                                                                                                                                                 <  1:1359745/286‑1 (MQ=255)
                                                                                                                          aTCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGATGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGAt                                                                                                                                                        >  1:1501847/1‑288 (MQ=255)
                                                                                                                           tCTGGCGCGACAGTGACAAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATcc                                                                                                                                                      >  2:769675/1‑289 (MQ=255)
                                                                                                                                cgcgACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCGAAATCGTGAAGGGGTGCATCTGAATGATGAGGAGCCGGccc                                                                                                                                                 >  1:465976/1‑289 (MQ=255)
                                                                                                                                    aCAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGcc                                                                                                                                                                                                                                    <  2:1893116/202‑1 (MQ=255)
                                                                                                                                         gACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGt                                                                                                                                             <  1:1480523/284‑1 (MQ=255)
                                                                                                                                               cGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGc                                                                                                                                  >  2:21969/1‑289 (MQ=255)
                                                                                                                                               cGTATGGCACAATTTACCGCTAGGCTGAACGGACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACg                                                                                                                                   <  2:908030/288‑1 (MQ=255)
                                                                                                                                                gTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCa                                                                                                                                 <  1:853434/289‑1 (MQ=255)
                                                                                                                                                 tATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCa                                                                                                                                 <  1:1011168/288‑1 (MQ=255)
                                                                                                                                                    ggCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCa                                                                                                                                                                        >  1:1223753/1‑246 (MQ=255)
                                                                                                                                                         aaTTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAATCGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGGGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAgg                                                                                                                        >  2:236618/1‑289 (MQ=255)
                                                                                                                                                         aaTTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGGGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGc                                                                                                                                        >  1:631166/1‑273 (MQ=255)
                                                                                                                                                           tttACCGCTCCTGTGAACGAACTGGGTTTACAGTTTATGCTGCGTGCGCGCGTCTGGCACGTACGTGATGCTTCTGCCGGAAAAGCTCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCGAACCAGACGTGGTCTGGGCGATGGGCCAAACGATGCGCCCGTCCTGGAGGTAATGGGTTATGCGGTGATTTTGAAAGGGCTAAATCGTGAAGGGGGGCGTCTGCATGATGAGGGTCCGGCCCGAGTCTGGCGAACACAGCGTGAAGGAc                                                                                                                      <  2:745218/289‑1 (MQ=255)
                                                                                                                                                                     gTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGt                                                                                                                                             <  1:539249/256‑1 (MQ=255)
                                                                                                                                                                     gTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGt                                                                                                                                             >  2:539249/1‑256 (MQ=255)
                                                                                                                                                                     gTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATg                                                                                                            >  2:982027/1‑289 (MQ=255)
                                                                                                                                                                     gTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATg                                                                                                            >  1:868688/1‑289 (MQ=255)
                                                                                                                                                                          aacgaacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTg                                                                                                       >  2:611843/1‑289 (MQ=255)
                                                                                                                                                                           acgaacTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTg                                                                                                       <  1:414717/288‑1 (MQ=255)
                                                                                                                                                                               acTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAggg                                                                                                  >  1:991477/1‑289 (MQ=255)
                                                                                                                                                                                    gCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTAcgcg                                                                                                                                                                                                           >  2:863131/1‑179 (MQ=255)
                                                                                                                                                                                              tATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCgg                                                                                                                                                                                                          <  2:398655/170‑1 (MQ=255)
                                                                                                                                                                                                tGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTtctc                                                                                 <  1:250235/289‑1 (MQ=255)
                                                                                                                                                                                                    gggTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAc                                                                                                                                                                                                                                                                        >  1:937571/1‑102 (MQ=255)
                                                                                                                                                                                                    gggTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGc                                                                              >  2:701116/1‑288 (MQ=255)
                                                                                                                                                                                                      gTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGACGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCg                                                                           >  2:1371506/1‑289 (MQ=255)
                                                                                                                                                                                                          gcgcTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGaa                                                                                                                                     <  2:947089/227‑1 (MQ=255)
                                                                                                                                                                                                                 tGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTa                                                                 >  2:67113/1‑288 (MQ=255)
                                                                                                                                                                                                                 tGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTAt                                                                <  1:956739/289‑1 (MQ=255)
                                                                                                                                                                                                                 tGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACC‑‑GTTTTCTCCGCCCGTTAAGCGTTAtc                                                               >  2:676561/1‑288 (MQ=255)
                                                                                                                                                                                                                     aCGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGACAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTAtcgct                                                            <  1:1876331/289‑1 (MQ=255)
                                                                                                                                                                                                                        tACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTAtcgctcgc                                                         >  2:463032/1‑289 (MQ=255)
                                                                                                                                                                                                                        tACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTAtcgctcgc                                                         >  2:1728111/1‑289 (MQ=255)
                                                                                                                                                                                                                        tACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTAtcgctcgc                                                         >  1:495721/1‑289 (MQ=255)
                                                                                                                                                                                                                        tACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTGCTCCGCCCGTTCAGCGTTAtcgctcgg                                                         >  2:298469/1‑288 (MQ=255)
                                                                                                                                                                                                                          cTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACCGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTTAAGGGGTGCATCTGCATGAGGAGGCTCCGGCCCGCGTCTGGCGTACGCAGCGTGAAGGACCGGAAGGATGGCGTGAATGGCTGGACCATGTTTTTTCCGCCCGGTAACCGTTACCGCTCGCG‑a                                                      >  2:1101241/1‑289 (MQ=255)
                                                                                                                                                                                                                             gATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AAt                                                    >  2:823094/1‑288 (MQ=255)
                                                                                                                                                                                                                               tGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATAcc                                                 <  1:1657448/289‑1 (MQ=255)
                                                                                                                                                                                                                                  ttCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACg                                                                                                                                   <  2:1639107/205‑1 (MQ=255)
                                                                                                                                                                                                                                  ttCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACg                                                                                                                                   >  1:1639107/1‑205 (MQ=255)
                                                                                                                                                                                                                                  ttCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCCGCGTGAAGGACCGGAAGGATGGCGGGAAGGGCCGGACCATTTTTTCTACGCCCGTTTAGCGTTGTCGCTCCCG‑attcaccga                                              >  2:682499/1‑281 (MQ=255)
                                                                                                                                                                                                                                  ttCTGCCGGAAAAGATCAGCCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATGGTGAAAGGGCTAAATCGGGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGGCCATTTTTTCTCTGCCCTTTAAGCGTTGTCGCT‑GCGCAATACCCGa                                              >  2:500277/1‑289 (MQ=255)
                                                                                                                                                                                                                                   tCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTCTCTCTCGCG‑AATACCCGaa                                             >  2:1775676/1‑288 (MQ=255)
                                                                                                                                                                                                                                    gtGCCGGAAAAGATCAGGCACCTAACTGGAGTATCGCTACCTATCAACAATTGGCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGAt                                             <  2:1659654/287‑1 (MQ=255)
                                                                                                                                                                                                                                       ccGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTAc                                          >  1:376321/1‑288 (MQ=255)
                                                                                                                                                                                                                                            aaaGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAg                                    <  1:823094/289‑1 (MQ=255)
                                                                                                                                                                                                                                             aaGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTATGCCCAGc                                   <  1:1775676/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         ccAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGCTGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCTCTCCGC‑AATACCCCATTACGCCCCGCCCGCTTTGCgag                       >  2:83151/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         ccAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCgag                       >  1:393382/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                   tATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCg              >  1:160408/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   tATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATAGTGAAAGGGCTAAATCGTGCAGGGGTGCATCTGCATGATGAGGCTCCGGCCCGCGTCCGGCGAACGCAGCGTGAAGCACAGGAATGATGGCGGGACGGGCTGGACCATTCTTTCTCCGCCCGTTAAGCGTTATCGCTCCCG‑AATACCCGATTACTCCACGCTCGTTTAGCgcaataccgctg              >  2:1054279/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                     tCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATACGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGGGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCAACg           >  1:1759230/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      cgcgACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTGTCCGTCCGTTCATCCTTATCGCTCGCG‑AATTCCCGATTACGGCCAGCCTGTTTAGCGAGATAAAGCCGACgg          >  2:1418993/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       gcgACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGt         <  1:769675/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gcgACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGt         <  2:376321/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aCCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTg                                                                                                                            <  2:1935921/172‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aCCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTg                                                                                                                            >  1:1935921/1‑172 (MQ=255)
                                                                                                                                                                                                                                                                          aCCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCAGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACTGTCg       >  1:1799637/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                            cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGTCGGcc    <  1:1098584/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cTATCAACAATTGGCAGGCAAAAGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTGACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGTCGGcc    <  2:1227761/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGTCGGCCa   <  1:21969/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                    
GAAAGAACATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGAGCGT‑AACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGAGTGGCAAACGCCCAACCACACTTGGCCTGGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGATTACGCGGTGATTGTGAAAGGGCTAAATCGTGAAGGGGTGCATCTGCATGATGAGGATCCGGCCCGCGTCTGGCGAACGCAGCGTGAAGGACCGGAAGGATGGCGTGAAGGGCTGGACCATTTTTTCTCCGCCCGTTAAGCGTTATCGCTCGCG‑AATACCCGATTACGCCCAGCCTGTTTAGCGAGATAAAGCCGACGGTCGGCCAG  >  CP042583/1663304‑1663861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A