Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F210 I1 R1	4928	368.7	6392010	97.5%	6232209	231.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	1,669,667	3 bp→CGA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	1,669,667	0	G	C	99.8%	961.7 / NA	403	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (190/211); total (192/211)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,669,668	0	C	G	99.3%	951.6 / ‑3.1	403	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (190/210); minor base T (2/1); total (192/211)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,669,669	0	T	A	99.8%	961.6 / NA	403	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (192/210); minor base C (0/1); total (192/211)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

ACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATGCTTTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCGCTGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTAACTATCGGTTACACTTATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGAACTAAACGCATCTATCACCTTGTGATCGA  >  CP042583/1669386‑1669947

|||

acGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGT‑ATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCAtt                                                                                                                                                                                                                                                                                  >  1:2121539/1‑289 (MQ=255)

           aTGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCa                                                                                                                                                                                                                                                                        <  1:1521210/289‑1 (MQ=255)

                  aGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTa                                                                                                                                                                                                                                                                 >  1:14490/1‑289 (MQ=255)

                  aGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTa                                                                                                                                                                                                                                                                 >  1:913244/1‑289 (MQ=255)

                  aGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTa                                                                                                                                                                                                                                                                 >  2:1044633/1‑289 (MQ=255)

                            cgcgGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCcg                                                                                                                                                                                                                                                        <  2:631188/288‑1 (MQ=255)

                            cgcgGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCcg                                                                                                                                                                                                                                                        <  1:2898284/288‑1 (MQ=255)

                              cgGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCcgcg                                                                                                                                                                                                                                                      >  2:2990263/1‑288 (MQ=255)

                                    tCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGt                                                                                                                                                                                                                                               >  1:1977777/1‑289 (MQ=255)

                                      ttCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAGGTTCGTACCGCATGTTTGTTCCTTTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGTCATGGATGCCGCCAATTCCGGCGAATAGATCTATAAAGCGAAACGTATAAAGCGGAAAATGCGTCGGTGGA‑TCGGGTAATAAAGTTTACAGATGGGCGAACACTATCTCACTTAAATGGTGCCATGTTTAGTCATTCGCCAGCACACTGTTTAAAATTgaaaagatcccgcca                                                                                                                                                                                                                                             >  2:3187771/1‑275 (MQ=255)

                                        cATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGAtt                                                                                                                                                                                                                                           <  1:3156756/289‑1 (MQ=255)

                                                tgatgCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTa                                                                                                                                                                                                                                   <  1:687390/289‑1 (MQ=255)

                                                tgatgCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTa                                                                                                                                                                                                                                   <  1:984288/289‑1 (MQ=255)

                                                                          gTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGtt                                                                                                                                                                                                         >  1:277682/1‑289 (MQ=255)

                                                                             ggCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTa                                                                                                                                                                                                      >  1:2710146/1‑289 (MQ=255)

                                                                               cTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGtttt                                                                                                                                                                                                       <  1:2529288/286‑1 (MQ=255)

                                                                                  tataAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAAcatc                                                                                                                                                                                                 >  2:661967/1‑289 (MQ=255)

                                                                                  tataAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAAcatc                                                                                                                                                                                                 >  2:2007107/1‑289 (MQ=255)

                                                                                    taAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGAtt                                                                                                                                                                                                                                           <  1:775415/245‑1 (MQ=255)

                                                                                        tGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTTCGCCACCAACGTTTTAACATCATAAAt                                                                                                                                                                                           >  1:2362426/1‑289 (MQ=255)

                                                                                         gCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGAtt                                                                                                                                                                                                                                           <  2:2747731/240‑1 (MQ=255)

                                                                                          cGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGAtt                                                                                                                                                                                                                                           <  1:1011308/239‑1 (MQ=255)

                                                                                          cGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAAttt                                                                                                                                                                                         >  2:2616450/1‑289 (MQ=255)

                                                                                          cGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAAATCTTTCTCACTTAAAAGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTGACCTCCACCATTAAGCTGCGCCACCAACGGTTTCACATCATAAAttt                                                                                                                                                                                         >  2:3043016/1‑289 (MQ=255)

                                                                                            tACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTgc                                                                                                                                                                                       >  2:1931472/1‑289 (MQ=255)

                                                                                            tACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCACTGATCCTCACCTACACCATTAAGCTGAGCCACCAACGTTTTAACATCATAAAttctc                                                                                                                                                                                       >  2:1871104/1‑286 (MQ=255)

                                                                                             aCCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTCAGCAGCGCCACTAACGTTTTAACAGCATAAATTTgca                                                                                                                                                                                      >  2:2154926/1‑289 (MQ=255)

                                                                                                 cATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGcagcag                                                                                                                                                                                  >  1:2592524/1‑289 (MQ=255)

                                                                                                 cATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGcagcag                                                                                                                                                                                  >  2:3166718/1‑289 (MQ=255)

                                                                                                 cATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGcagcag                                                                                                                                                                                  >  2:2339136/1‑289 (MQ=255)

                                                                                                  aTGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGTTTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGG‑TTGGGTACTAACGTTTGCAGATGGGCGAAGTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCTGCGAACGTTTTAAAATTGCGGAGCGCCAGTGATTATCACCGTCCACATTAAGCTGCCCCAACACGTTTTTAACCTCATAAATTTccagcata                                                                                                                                                                                 >  2:2202135/1‑287 (MQ=255)

                                                                                                    gtttgttCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGttt                                                                                                                                                                               >  2:1435554/1‑289 (MQ=255)

                                                                                                      ttgttCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTtct                                                                                                                                                                             <  1:716698/289‑1 (MQ=255)

                                                                                                        gttCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTcc                                                                                                                                                                           <  2:2710146/289‑1 (MQ=255)

                                                                                                        gttCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTcc                                                                                                                                                                           <  1:2616450/289‑1 (MQ=255)

                                                                                                           ccATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGc                                                                                                                                                                        <  1:619240/289‑1 (MQ=255)

                                                                                                             aTTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCaa                                                                                                                                                                                                             <  1:3130427/250‑1 (MQ=255)

                                                                                                               tCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCAtt                                                                                                                                                                     >  2:756751/1‑288 (MQ=255)

                                                                                                                cGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCcg                                                                                                                                                                                                                                                        <  2:943004/204‑1 (MQ=255)

                                                                                                                         acacGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGc                                                                                                                                                          <  2:2433055/289‑1 (MQ=255)

                                                                                                                           acGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGccacc                                                                                                                                                                                                               >  2:2861084/1‑234 (MQ=255)

                                                                                                                               aCTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGAtt                                                                                                                                                                                                                                           <  2:2110522/202‑1 (MQ=255)

                                                                                                                               aCTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGtttt                                                                                                                                                                                                       <  2:491630/238‑1 (MQ=255)

                                                                                                                               aCTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTcc                                                                                                                                                     <  1:1159443/288‑1 (MQ=255)

                                                                                                                                   tCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCcg                                                                                                                                                                                                                                                        >  2:1397057/1‑185 (MQ=255)

                                                                                                                                   tCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGAtt                                                                                                                                                                                                                                           <  1:857224/198‑1 (MQ=255)

                                                                                                                                   tCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTcc                                                                                                                                                     >  1:141268/1‑284 (MQ=255)

                                                                                                                                    ccGCCAATCGATTCCAAAGCGGGGCGGATGCCGGCAAGTCCGGCGAATAGGTCGGTAAAGCGAAACGCATGATGCGGATGATGTGGCGGTTGT‑TTGGGTAATAACGTTTGCATATGGGCGAACTCTTTCTCACTTAAACGGGGCCATGCCGAGTCAGTCGCCAGCGAAAGTGTTAAAATTGCCGCGGTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTGTAACATCATAAATTTGCAGCAGTTTGTCCAGCATTGCCTGTGCGGCATTCCCGGTg                                                                                                                                                <  2:2823995/288‑1 (MQ=255)

                                                                                                                                    ccGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTg                                                                                                                                                <  1:3097090/288‑1 (MQ=255)

                                                                                                                                             gcttCAAAACCGAGACGGATGCCGCAAAGTCCGGCGAATAGTTCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTatgaat                                                                                                                                      <  2:2283170/287‑6 (MQ=255)

                                                                                                                                               ttCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTtct                                                                                                                                                                             >  1:1867164/1‑248 (MQ=255)

                                                                                                                                               ttCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTatgaatc                                                                                                                                     <  2:1488336/288‑7 (MQ=255)

                                                                                                                                                         cgACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCa                                                                                                                                                                                                                                                                        >  1:1694796/1‑147 (MQ=255)

                                                                                                                                                         cgACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCa                                                                                                                                                                                                                                                                        <  2:1694796/147‑1 (MQ=255)

                                                                                                                                                         cgACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTactgata                                                                                                                          >  1:1355109/1‑284 (MQ=255)

                                                                                                                                                                tGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTc                                                                                                                   <  1:1918003/289‑1 (MQ=255)

                                                                                                                                                                         ttCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTatgaatcg                                                                                                                                    <  2:1806875/263‑8 (MQ=255)

                                                                                                                                                                                  aTAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCgg                                                                                                 <  1:110117/289‑1 (MQ=255)

                                                                                                                                                                                  aTAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCgg                                                                                                 <  1:3043016/289‑1 (MQ=255)

                                                                                                                                                                                       tCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCAt                                                                                             <  1:1381166/288‑1 (MQ=255)

                                                                                                                                                                                         gATAACGCGGAACGCATTATGCGGATGATTGTCCGGTAGT‑ATGGGTAATAAAGTTGGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTGTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAATGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTg                                                                                          <  2:610602/289‑1 (MQ=255)

                                                                                                                                                                                           tAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTa                                                                                                                                                                                                                                   >  1:386063/1‑150 (MQ=255)

                                                                                                                                                                                           tAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTa                                                                                                                                                                                                                                   <  2:386063/150‑1 (MQ=255)

                                                                                                                                                                                                   aaCGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGa                                                                                <  2:313054/289‑1 (MQ=255)

                                                                                                                                                                                                     cGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCgg                                                                                                 <  1:2273719/270‑1 (MQ=255)

                                                                                                                                                                                                        aTAATTCGGGTGATGTGCCGGTGGTGTTGGGT‑ATAACGGTTGCAGATGGGCGAACTCTTTCTCACTTATACGGTGCAATGCCGAGTCATTCGCCAGCGCACGTTTTAACATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAGATTTGCAGCAGTTTCTCCATCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGGGATGGCCGACAGGt                                                                           <  2:135794/289‑1 (MQ=255)

                                                                                                                                                                                                           aTGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTaa                                                                        <  2:2561100/289‑1 (MQ=255)

                                                                                                                                                                                                               ggATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTc                                                                                                                   <  1:476398/242‑1 (MQ=255)

                                                                                                                                                                                                                 atgatgTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTactgatata                                                                                                                        <  1:3173685/235‑8 (MQ=255)

                                                                                                                                                                                                                 atgatgTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTactgatata                                                                                                                        >  2:3173685/1‑228 (MQ=255)

                                                                                                                                                                                                                      gtgCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAg                                                             <  2:1640270/289‑1 (MQ=255)

                                                                                                                                                                                                                       tgCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAg                                                             <  2:1139098/288‑1 (MQ=255)

                                                                                                                                                                                                                         ccGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCg                                                                                                  >  2:1771550/1‑249 (MQ=255)

                                                                                                                                                                                                                         ccGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCg                                                                                                  <  1:1771550/249‑1 (MQ=255)

                                                                                                                                                                                                                           ggtggt‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGAAAGGTTAACATAATTACa                                                              >  1:2099009/1‑283 (MQ=255)

                                                                                                                                                                                                                               gt‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAg                                                                                                                                                                                                                                                >  2:871155/1‑101 (MQ=255)

                                                                                                                                                                                                                                    gggTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATTCCGAGTCATTCGCAAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCa                                                <  1:1564084/289‑1 (MQ=255)

                                                                                                                                                                                                                                    gggTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCa                                                <  1:582441/289‑1 (MQ=255)

                                                                                                                                                                                                                                        actaaCGTTTGCAGATGGGCGAAATAGTTCTACCTTAAACGGTGCCATTCCGCGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTTATTCTCACCTACACCATTAAGCTGCGCAACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGGTAACATAATTACAGCCATTTAGGGCCAcgcg                                            <  2:3080551/287‑1 (MQ=255)

                                                                                                                                                                                                                                          taaCGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTc                                                                                                                   >  2:2383218/1‑216 (MQ=255)

                                                                                                                                                                                                                                              gTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagc                                      <  1:2451751/289‑1 (MQ=255)

                                                                                                                                                                                                                                              gTTTGCAGATGGGCGAACTCTATCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagc                                      <  1:3187771/289‑1 (MQ=255)

                                                                                                                                                                                                                                               tttGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCg                                                                                                  <  1:1656443/228‑1 (MQ=255)

                                                                                                                                                                                                                                               tttGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCAt                                                                                             <  1:662368/233‑1 (MQ=255)

                                                                                                                                                                                                                                               tttGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGACACGCGCTcagcc                                     >  1:1783901/1‑289 (MQ=255)

                                                                                                                                                                                                                                                 tGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGt                                                                                                                                  >  2:422541/1‑194 (MQ=255)

                                                                                                                                                                                                                                                 tGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGt                                                                                                                                  <  1:422541/194‑1 (MQ=255)

                                                                                                                                                                                                                                                 tGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagccag                                   <  1:2183118/289‑1 (MQ=255)

                                                                                                                                                                                                                                                 tGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagcca                                    >  1:2844834/1‑288 (MQ=255)

                                                                                                                                                                                                                                                        gggCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGAATTCACCGGCCATCTgaga                                                                                       >  2:25011/1‑230 (MQ=255)

                                                                                                                                                                                                                                                          gCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGatgaa                           <  2:809596/288‑5 (MQ=255)

                                                                                                                                                                                                                                                                    tctcACTTAAACGGTGCCATGCCTAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTgaga                                                                                       >  1:2971349/1‑218 (MQ=255)

                                                                                                                                                                                                                                                                    tctcACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGAGGAAAACGCATCTa                 >  2:1375376/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                          ttAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCTCCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTactgatata                                                                                                                        >  2:2922798/1‑172 (MQ=255)

                                                                                                                                                                                                                                                                          ttAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCa                                                <  2:2605638/251‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  tGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGt                                                                                                                                  <  2:1334753/161‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  tGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGt                                                                                                                                  >  1:1334753/1‑161 (MQ=255)

                                                                                                                                                                                                                                                                                  tGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCAt                                                                                             >  2:705740/1‑198 (MQ=255)

                                                                                                                                                                                                                                                                                  tGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGa                                                                                >  1:2316924/1‑211 (MQ=255)

                                                                                                                                                                                                                                                                                   gCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGa  <  1:2397890/288‑1 (MQ=255)

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ACGCCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATGCTTTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGT‑TTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCGCTGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTAACTATCGGTTACACTTATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGAACTAAACGCATCTATCACCTTGTGATCGA  >  CP042583/1669386‑1669947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A