Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,969,207 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,969,2070GC99.0% 694.8 / NA 298intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base C (145/150);  total (145/153)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,969,2080CG99.0% 691.8 / NA 298intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (143/151);  total (145/153)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,969,2090TA99.7% 703.8 / NA 298intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (143/153);  total (145/153)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 299 total aligned reads displayed.

GTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGACTCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGGCTACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATGCTGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAG  >  CP042583/1968930‑1969491
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                           
      tGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCgg                                                                                                                                                                                                                                                                              <  2:3116885/289‑1 (MQ=255)
       gTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGc                                                                                                                                                                                                                                                                             <  1:3172430/289‑1 (MQ=255)
       gTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGc                                                                                                                                                                                                                                                                             <  1:1505337/289‑1 (MQ=255)
        tACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCCGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGc                                                                                                                                                                                                                                                                             <  2:2798033/288‑1 (MQ=255)
           ggCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAAGTTGTTCCCCGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGGGCGCAGTCCGCCGgt                                                                                                                                                                                                                                                                         >  2:2994298/1‑289 (MQ=255)
                  gCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACTGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTa                                                                                                                                                                                                                                                                    >  1:1211418/1‑287 (MQ=255)
                            gtGGAAGACCGGAGGACGCTGTGACAATTCATCGCGGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCAtcgag                                                                                                                                                                                                                                                        <  2:2625682/288‑5 (MQ=255)
                            ctGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTTGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCaccgag                                                                                                                                                                                                                                                        <  1:957563/289‑6 (MQ=255)
                            ctGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCAtcgag                                                                                                                                                                                                                                                        <  2:1617153/289‑5 (MQ=255)
                                    ccGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGaa                                                                                                                                                                                                                                                <  2:1177239/289‑1 (MQ=255)
                                          agacaggggacAATTCATCCCTCCCAAACAGGGGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATgg                                                                                                                                                                                                                                           <  2:2833115/278‑1 (MQ=255)
                                               tgtgacAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGCAGGGGAACATGGTGTcca                                                                                                                                                                                                                                     >  1:929414/6‑287 (MQ=255)
                                                  gacAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAata                                                                                                                                                                                                                                   >  1:3057177/3‑288 (MQ=255)
                                                  gacAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGACCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGGCAata                                                                                                                                                                                                                                   >  1:1060960/3‑288 (MQ=255)
                                                         cATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCAtcgag                                                                                                                                                                                                                                                        <  1:2890209/260‑5 (MQ=255)
                                                                 ccAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcgcg                                                                                                                                                                                                                   >  2:1095825/1‑289 (MQ=255)
                                                                         gtggtAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCCTCGAGAAGGGGAAAATGTGGTCAAAATCCTGCGGCTGACCCGGGTCTTGa                                                                                                                                                                                                           >  2:1713565/1‑289 (MQ=255)
                                                                          tggtAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATg                                                                                                                                                                                                            <  1:1611075/287‑1 (MQ=255)
                                                                                 aCGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCAtcgagaagg                                                                                                                                                                                                                                                    <  1:1482036/240‑9 (MQ=255)
                                                                                    gcgcGATACGCCATTTCCCGTCCTGCGGCTGGACGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATCTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAat                                                                                                                                                                                                                                    >  1:2225454/1‑253 (MQ=255)
                                                                                    gcgcGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACAt                                                                                                                                                                                                                                             >  1:3131258/1‑244 (MQ=255)
                                                                                    gcgcGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcg                                                                                                                                                                                                                     <  2:895919/268‑1 (MQ=255)
                                                                                    gcgcGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATAtt                                                                                                                                                                                                >  1:2136046/1‑289 (MQ=255)
                                                                                     cgcgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGGGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGGCAATATCCTGCGGCTCACGCGGCGCATGAGCGTGGATAttt                                                                                                                                                                                               >  2:2421234/1‑288 (MQ=255)
                                                                                      gcgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTgg                                                                                                                                                                                                                                                                          >  1:446310/1‑213 (MQ=255)
                                                                                       cgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACa                                                                                                                                                                                                                                              <  1:2712644/240‑1 (MQ=255)
                                                                                       cgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACa                                                                                                                                                                                                                                              >  2:2712644/1‑240 (MQ=255)
                                                                                       cgATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCGCATGAACGCTGATATTGgc                                                                                                                                                                                             >  1:1431184/1‑289 (MQ=255)
                                                                                                   ttccGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCTCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAAc                                                                                                                                                                                                                                               <  1:1658773/225‑1 (MQ=255)
                                                                                                         ccTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGGGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            <  1:1902074/288‑1 (MQ=255)
                                                                                                         ccTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            <  1:2176857/288‑1 (MQ=255)
                                                                                                            gCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcg                                                                                                                                                                                                                     >  2:2283980/1‑244 (MQ=255)
                                                                                                             cGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGc                                                                                                                                                                                                                                                                             <  2:1082332/187‑1 (MQ=255)
                                                                                                             cGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCAtcgag                                                                                                                                                                                                                                                        >  2:421600/1‑204 (MQ=255)
                                                                                                             cGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGa                                                                                                                                                                                                           <  2:2374072/253‑1 (MQ=255)
                                                                                                             cGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTgg                                                                                                                                                                                              <  1:2961544/266‑1 (MQ=255)
                                                                                                             cGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAAACGCACGCCCGGATCGCCCAAGCGCAGATTGCCGCCCACTTAGTCCTGGAATTTGTTCCCCGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCACACGGCACTTTCCACCGGTGCGCGGTCCGCTGGTTTTGACCTTCCGTCCAGGAGGCCAAAATGCTGTCaa                                                                                                                                                                                                                                     >  2:1658773/1‑227 (MQ=255)
                                                                                                                 tGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTaa                                                                                                                                                                    <  1:1127649/288‑1 (MQ=255)
                                                                                                                     aggcacgcGGTACGCTGGTGGAGTAATCCAGACCATTTTCGCTGGTTTAAAACAGACGCCCGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTg                                                                                                                                                               <  2:2035665/282‑1 (MQ=255)
                                                                                                                       cggtccGGTACGCTGGTGAAGTAATCCAGACCAGTTTCGCGGGTTTCAACAGGAGGCACGCCCGGATCGCCAAGGGGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTg                                                                                                                                                               <  2:2062841/283‑1 (MQ=255)
                                                                                                                         tGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTTGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCACTATCCTGCTGATGACGCTGCTCATGAACGCTGATATTGGCGAGCATGGCGGTACGACCGCTGTAACGGttccgt                                                                                                                                                           >  2:371304/1‑284 (MQ=255)
                                                                                                                         tGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCt                                                                                                                                                                                                                         >  1:864730/1‑227 (MQ=255)
                                                                                                                                      gTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGAcg                                                                                                                                                                                                                     >  1:2113372/1‑218 (MQ=255)
                                                                                                                                              agccagggcctgttCGCTGGTTTCAAACGGACGCACGCCCGGATCGCCAAAGCGCAGTGTGCCGCCCACTTCGTCCTGGAAGTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGGGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCa                                                                                                                                     <  2:3057177/277‑1 (MQ=255)
                                                                                                                                              aTCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAACGCGCAGTTTGCCGCCCACTTCGTCCTCGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCCAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGCGACGGTGAACATGTTGGCAATTTCCTTCGGCTGACGCGTCCCCTGACGGCTGATATTGGCCTGCATGGCGGTACGACCCCTGTAACGGTGC‑GTTCACCGGTCTCGTTTCTGccc                                                                                                                                      >  2:3178581/1‑288 (MQ=255)
                                                                                                                                              aTCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGccc                                                                                                                                      >  2:3135830/1‑288 (MQ=255)
                                                                                                                                              aTCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGccc                                                                                                                                      >  2:825011/1‑288 (MQ=255)
                                                                                                                                               tCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCAc                                                                                                                                    <  1:341610/289‑1 (MQ=255)
                                                                                                                                                 cAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACAt                                                                                                                                                                                                                                             >  1:277020/1‑183 (MQ=255)
                                                                                                                                                    aCCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAAt                                                                                                                                >  1:293834/1‑288 (MQ=255)
                                                                                                                                                        ttttCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCGAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAAt                                                                                                                                >  1:2051593/1‑284 (MQ=255)
                                                                                                                                                        ttttCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGGAACGGTGCGGTTCA‑TGGGCCCGTTTCTGCCCACGACTACGGa                                                                                                                           >  2:1151798/1‑289 (MQ=255)
                                                                                                                                                              cTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCAt                                                                                                                                                                                                             >  1:2348800/1‑202 (MQ=255)
                                                                                                                                                                             cgcacgcCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgcagc                                                                                                        <  1:1052580/287‑1 (MQ=255)
                                                                                                                                                                             cgcacgcCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgca                                                                                                          <  1:1683143/285‑1 (MQ=255)
                                                                                                                                                                                      ggATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGAAATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATa                                                                                              >  1:2613047/1‑288 (MQ=255)
                                                                                                                                                                                       gATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            <  1:2825779/210‑1 (MQ=255)
                                                                                                                                                                                       gATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATa                                                                                              >  1:1576756/1‑287 (MQ=255)
                                                                                                                                                                                        aTCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATg                                                                                                                                                                                                            >  1:2027587/1‑177 (MQ=255)
                                                                                                                                                                                          cGCCAACGCGCAGTTTGCCGCCCACTTCGTCCTGGCATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGc                                                                                         <  1:371304/289‑1 (MQ=255)
                                                                                                                                                                                          cGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTTA‑CGGGc                                                                                                                                                  >  2:1380397/1‑232 (MQ=255)
                                                                                                                                                                                            ccAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATAccgggca                                                                                       >  1:239137/1‑284 (MQ=255)
                                                                                                                                                                                              aaaGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            >  1:2550677/1‑203 (MQ=255)
                                                                                                                                                                                                  cgcAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            <  1:2600780/199‑1 (MQ=255)
                                                                                                                                                                                                  cgcAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            >  2:2600780/1‑199 (MQ=255)
                                                                                                                                                                                                      gTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACAt                                                                                                                                                                                                                                             <  1:242144/130‑1 (MQ=255)
                                                                                                                                                                                                       tttGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                            >  1:2319469/1‑289 (MQ=255)
                                                                                                                                                                                                       tttGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCCCAGCATCTTTTATCCCTGCCAGTTCCGGGagt                                                                            >  2:1570590/1‑287 (MQ=255)
                                                                                                                                                                                                        ttGCCGCCCACTTCGTCCTGGACTTTGTCCCCCGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                            <  2:502853/288‑1 (MQ=255)
                                                                                                                                                                                                                   ttCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCAt                                                                                                                                                                                       >  2:2457661/1‑171 (MQ=255)
                                                                                                                                                                                                                   ttCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                            >  1:1088751/1‑277 (MQ=255)
                                                                                                                                                                                                                     cGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGc                                                              <  2:1861851/289‑1 (MQ=255)
                                                                                                                                                                                                                        ccTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCAt                                                                                                                                                                                       >  2:3148325/1‑166 (MQ=255)
                                                                                                                                                                                                                        ccTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCAt                                                                                                                                                                                       <  1:3148325/166‑1 (MQ=255)
                                                                                                                                                                                                                        ccTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGAc                                                                                                                                                                            <  2:2165739/177‑1 (MQ=255)
                                                                                                                                                                                                                        ccTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGCATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTc                                                           >  1:1187515/1‑289 (MQ=255)
                                                                                                                                                                                                                        ccTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGCTCGGTTCA‑CGGGCCAGTTTCTTCCCACGACTACGGAATGTCCCATCCGGCGCAGCATCCTTGATCCCTTCCAGTTCCGGGGTTTTCGCCCCACCAGCGt                                                            >  2:2220093/1‑288 (MQ=255)
                                                                                                                                                                                                                                 tGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATg                                                  >  1:1164682/1‑289 (MQ=255)
                                                                                                                                                                                                                                    tCCACGCCTGCGGTGAGTTCCGGCCCGGCGCCAAGGCAAACGGAACTTGCGAACGGGGCGCAGTCGGCTGGTAGTTACCTTCCGTCGAGAAGGTGAAAATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTc                                               <  2:1209413/289‑1 (MQ=255)
                                                                                                                                                                                                                                             gCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCggg                                                                               >  2:2352515/1‑248 (MQ=255)
                                                                                                                                                                                                                                             gCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGGCAATCACATGGTCGAGCTGCg                                       >  1:1742755/1‑288 (MQ=255)
                                                                                                                                                                                                                                                   aGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCt                                          >  2:1898352/1‑279 (MQ=255)
                                                                                                                                                                                                                                                   aGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCCGCCCACGAATACGGAATGTACCATCCGGCGCAGCATCTTTTATACCTTCCAGTTCCGGGCTCTCCGCCACAACAGCGTCAATCACAATGTCGGGCTGCccccagt                                 >  2:1213684/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    gTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtc                                <  1:2497611/288‑1 (MQ=255)
                                                                                                                                                                                                                                                     ttCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtcc                               >  1:135730/1‑288 (MQ=255)
                                                                                                                                                                                                                                                       ccAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGc                                                                                                                                                                                                       >  2:1328629/1‑119 (MQ=255)
                                                                                                                                                                                                                                                       ccAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCAc                                                                                                                                    <  2:1006749/185‑1 (MQ=255)
                                                                                                                                                                                                                                                       ccAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCAc                                                                                                                                    >  1:1006749/1‑185 (MQ=255)
                                                                                                                                                                                                                                                       ccAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtc                                     <  1:2735976/280‑1 (MQ=255)
                                                                                                                                                                                                                                                       ccAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCCGCgtc                                     >  2:2735976/1‑280 (MQ=255)
                                                                                                                                                                                                                                                          gCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCa                         <  2:1052914/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          cccccGCGCCCAGGCAAACGGAACTAGCTAACGGTGCGCATTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTACCGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGACTGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCa                         <  1:1386639/288‑1 (MQ=255)
                                                                                                                                                                                                                                                               gcgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTg                                                                                                                                         <  1:1876159/172‑1 (MQ=255)
                                                                                                                                                                                                                                                               gcgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACg                       <  2:2951108/286‑1 (MQ=255)
                                                                                                                                                                                                                                                               gcgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGc                     >  1:1028999/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    cAGGCGACCTGCACTTGCGAACGGTGCGTCGTCGGCTGGTTGTTCCCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTcagca               <  2:2113925/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTcagca               <  1:2360199/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aaaCGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTg          <  1:651034/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ggCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCgcagc                                                                                                        <  2:938635/191‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 agtGGCAACGGTGCGCAGCCGGCTGGTGTTTACCTTCCAACGGGAAGGTGAAGATGGTGTCAATATCCGGCGGCTGCGGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGCGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGTATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAg  <  2:1431184/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                           
GTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGACTCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGATCGCCAAAGCGCAGTTTGCCGCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGGCTACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATGCTGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCA‑CGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAG  >  CP042583/1968930‑1969491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A