Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,528,162 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,528,1620AT100.0% 630.8 / NA 265intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (122/143);  total (122/143)
*CP0425831,528,1630CG99.6% 621.1 / NA 265intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (122/142);  minor base A (0/1);  total (122/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,528,1640TA99.6% 624.9 / NA 265intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (121/143);  minor base C (1/0);  total (122/143)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 265 total aligned reads displayed.

CCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCACTAAGGCTAAAAGGCAGCGGCGCGGATTCACTTTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGT  >  CP042583/1527885‑1528441
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                     
ccTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAACTAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGCTTCTGGCAGATATCAAGCACGTTCTGCGCACGCAGACCAAACCGTTTTCACGCGTCAATCTGCAACGCCGAAAGCGAAAAAGGCAGCGGCGCGGTTTCTGATTCACGttt                                                                                                                                                                                                                                                                              >  2:1989062/1‑289 (MQ=255)
ccTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGTTAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGttt                                                                                                                                                                                                                                                                              >  1:440275/1‑289 (MQ=255)
ccTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGttt                                                                                                                                                                                                                                                                              <  1:1382765/289‑1 (MQ=255)
ccTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGtt                                                                                                                                                                                                                                                                               <  1:1891235/288‑1 (MQ=255)
           tCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTta                                                                                                                                                                                                                                                                       <  2:127506/285‑1 (MQ=255)
               aaCAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGt                                                                                                                                                                                                                                                               <  1:1162711/289‑1 (MQ=255)
                             ataaCTGGATCTCACACCGTCCGGGGCAACAGATCCGGTGCATGGACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGtt                                                                                                                                                                                                                                                 <  2:1474750/289‑1 (MQ=255)
                                    gATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACt                                                                                                                                                                                                                                          >  1:779029/1‑289 (MQ=255)
                                    gATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACt                                                                                                                                                                                                                                          >  1:1585386/1‑289 (MQ=255)
                                         aCCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGttt                                                                                                                                                                                                                                                                              <  2:1648536/248‑1 (MQ=255)
                                         aCCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGAAGCGGCGCGGCTTCTGATTCCAGTTTCTCGTTATAGCTGGTGAAAATAGCAGGTTGCACACTAAAGc                                                                                                                                                                                                                                     >  2:149237/1‑289 (MQ=255)
                                            aCTGGCTGCGGGCACATATCCGGTGCATGAACACTGAGGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGt                                                                                                                                                                                                                                  <  2:1712529/289‑1 (MQ=255)
                                              ttgCTGCGGCAACAGATCCGGTGCATGAACACTGAGGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTa                                                                                                                                                                                                                                <  2:795394/287‑1 (MQ=255)
                                              tGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTa                                                                                                                                                                                                                                >  1:90832/1‑289 (MQ=255)
                                                gCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCTTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACTCGGATAACTGATTAGCTTGTGCGTTTCGTAAAGTTTCTGACAGATATCAAGCACGTTCTGCGCACTCATACCAAAACGTTTTGCCGCTTCAATCTGCAACGGTGAATCCGAAAAAGGCAGCGGCGCGGATTCTGACTCCCGTTTAAGATTATTGCTGGTGAAAATAGCCGGTTGACCACTAACGCAGTTaa                                                                                                                                                                                                                               >  2:1172227/1‑288 (MQ=255)
                                                    cGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGtt                                                                                                                                                                                                                                                                               >  1:38241/1‑236 (MQ=255)
                                                     ggCAACAAATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACcaca                                                                                                                                                                                                                          <  1:594484/288‑1 (MQ=255)
                                                         aCAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTc                                                                                                                                                                                                                     <  1:1009931/289‑1 (MQ=255)
                                                             gacaggTGCAGTAACCCGGATGGCATTCAGCACCTCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAGCGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGGTGAAAGCGAAAAAGGCAGCGGGGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGc                                                                                                                                                                                                                  <  2:492865/284‑1 (MQ=255)
                                                              tCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGc                                                                                                                                                                                                                  <  1:942181/287‑1 (MQ=255)
                                                                    tcatagacacTAATGGCATTCCTCACCGCGTGGCGGCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGGACGCGGATGAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGa                                                                                                                                                                                                           <  2:1628878/282‑1 (MQ=255)
                                                                    gCATGAACACTGATGGCATTCAACACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATCCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGAc                                                                                                                                                                                                          <  2:572307/289‑1 (MQ=255)
                                                                     cATGAACACTGATGGCAGTCATGACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCGAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGAc                                                                                                                                                                                                          <  2:35396/288‑1 (MQ=255)
                                                                             cTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAACGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGa                                                                                                                                                                                                        >  1:492865/1‑282 (MQ=255)
                                                                             cTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGttt                                                                                                                                                                                                                                                                              <  1:1752628/212‑1 (MQ=255)
                                                                             cTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACa                                                                                                                                                                                                 >  2:1159931/1‑289 (MQ=255)
                                                                                aTGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAgc                                                                                                                                                                                              >  1:453170/1‑289 (MQ=255)
                                                                                 tGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAgcg                                                                                                                                                                                             <  1:1524272/289‑1 (MQ=255)
                                                                                    cagtcatcaCCGGGTGGAGTGCGGCAAAAGGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGAGAAGTGAGTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGAGATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCATTCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGcc                                                                                                                                                                                           <  2:1683064/285‑1 (MQ=255)
                                                                                      ttCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCctt                                                                                                                                                                                        >  2:59467/1‑289 (MQ=255)
                                                                                        catcaCCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTaa                                                                                                                                                                                                                                        <  2:26138/239‑1 (MQ=255)
                                                                                        catcaCCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttct                                                                                                                                                                                      >  1:1170341/1‑289 (MQ=255)
                                                                                        catcaCCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttct                                                                                                                                                                                      <  1:572981/289‑1 (MQ=255)
                                                                                        catcaCCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttct                                                                                                                                                                                      <  2:1349857/289‑1 (MQ=255)
                                                                                         atcaCCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttctt                                                                                                                                                                                     <  2:655248/289‑1 (MQ=255)
                                                                                         atcaCCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttctt                                                                                                                                                                                     <  1:1706443/289‑1 (MQ=255)
                                                                                             ccGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGCACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATc                                                                                                                                                                                 <  2:643912/289‑1 (MQ=255)
                                                                                                cgTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATTTCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTg                                                                                                                                                                              >  1:1726535/1‑289 (MQ=255)
                                                                                                cgTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATg                                                                                                                                                                                                                                      <  2:1972737/233‑1 (MQ=255)
                                                                                                cgTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATg                                                                                                                                                                                                                                      >  1:1972737/1‑233 (MQ=255)
                                                                                                cgTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTc                                                                                                                                                                                                                     <  1:1041765/250‑1 (MQ=255)
                                                                                                cgTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGa                                                                                                                                                                                                           >  2:705696/1‑260 (MQ=255)
                                                                                                   ggCGTCCGGCAACATGTTCTTCTGGCAAATAGCGACGATCAGAACGCGGATAAGTGATTAGCTTGTGCGTCTCGTACAGTTTCTGGCAGATCTCAAGCACGTTCCCCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCACCGCTGAAAGCGCAAAAGGCAGCCGGGTGGCCTCTGCTTCTCGCTTCACGTTACAGTGCCTGCGAATAGCCGGTTGACCACTAATGCGGTTAACGACATGCTCCGCCAGTGGACGAGGTAACAAGCGACGTTATTCATccctgta                                                                                                                                                                           >  2:234916/1‑284 (MQ=255)
                                                                                                   ggCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGt                                                                                                                                                                            <  2:916404/288‑1 (MQ=255)
                                                                                                     cGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTAc                                                                                                                                                                          <  2:1919034/288‑1 (MQ=255)
                                                                                                       tCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGt                                                                                                                                                                       >  1:845410/1‑289 (MQ=255)
                                                                                                            cAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTcac                                                                                                                                                                   >  1:1780701/1‑288 (MQ=255)
                                                                                                                                tAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                                                                                                                                              <  1:646729/289‑1 (MQ=255)
                                                                                                                                tAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                                                                                                                                              <  1:1449427/289‑1 (MQ=255)
                                                                                                                                tAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                                                                                                                                              <  1:1993525/289‑1 (MQ=255)
                                                                                                                                tAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCACCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                                                                                                                                              <  2:484854/289‑1 (MQ=255)
                                                                                                                                tAGCGAAAATCAGAACCCGGATAAGTGGTTAGCTTGGGCGTTTCGTCCAGTTTCTGCCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                                                                                                                                              <  2:1120739/289‑1 (MQ=255)
                                                                                                                                                    aTAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGcc                                                                                                                                                                                                                                                     >  1:1431018/1‑166 (MQ=255)
                                                                                                                                                    aTAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGcc                                                                                                                                                                                                                                                     <  2:1431018/166‑1 (MQ=255)
                                                                                                                                                      aaGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGc                                                                                                                        <  1:1671146/289‑1 (MQ=255)
                                                                                                                                                         tGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAg                                                                                                                      <  2:1777115/288‑1 (MQ=255)
                                                                                                                                                          gATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAg                                                                                                                      <  1:149237/287‑1 (MQ=255)
                                                                                                                                                          gATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGgt                                                                                                                    <  1:1071386/289‑1 (MQ=255)
                                                                                                                                                            ttAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGgtgt                                                                                                                  <  1:667254/289‑1 (MQ=255)
                                                                                                                                                              aGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAACGGCAGCGGCGCGGCTTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCCCCGCCAGTGGACGATGTAACAATCTCCCTTCTTCATCCTGGTACGGTTCACACCCTTCGCTCGGTTGCCAGATAGCGGTAACCCTCCCATCGGCAGGTGTc                                                                                                                 >  2:1652902/1‑288 (MQ=255)
                                                                                                                                                              aGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttctt                                                                                                                                                                                     <  1:985315/220‑1 (MQ=255)
                                                                                                                                                                cTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCAt                                                                                                                                                                                  >  1:1283202/1‑221 (MQ=255)
                                                                                                                                                                 ttGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGa                                                                                                             <  1:683150/289‑1 (MQ=255)
                                                                                                                                                                  tgtgCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGat                                                                                                            >  1:115409/1‑289 (MQ=255)
                                                                                                                                                                   gtgCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGata                                                                                                           >  1:425440/1‑289 (MQ=255)
                                                                                                                                                                    tgCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAg                                                                                                                                                                                               >  1:543332/1‑204 (MQ=255)
                                                                                                                                                                     gCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCTGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGatat                                                                                                          <  2:779029/288‑1 (MQ=255)
                                                                                                                                                                     gCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATAtg                                                                                                         >  1:822551/1‑289 (MQ=255)
                                                                                                                                                                          tcttACAGTTTCTGCCAGATAGCATGCACGTTCTGCGACCTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATTGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCtt                                                                                                    <  2:440072/286‑1 (MQ=255)
                                                                                                                                                                          tCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCtt                                                                                                    >  2:979775/1‑289 (MQ=255)
                                                                                                                                                                           cGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCAt                                                                                                                                                                                  <  2:1873358/210‑1 (MQ=255)
                                                                                                                                                                           cGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCAt                                                                                                                                                                                  >  1:1873358/1‑210 (MQ=255)
                                                                                                                                                                           cGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCttt                                                                                                   <  1:1195038/289‑1 (MQ=255)
                                                                                                                                                                                aGTTTCTGGCAGGTATCAAGCACGTTCTGCGCACTCAGACCAACACGTGTTGCCGCTTCAACCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACtt                                                                                              <  2:407737/289‑1 (MQ=255)
                                                                                                                                                                                     cTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAaag                                                                                         <  2:120299/289‑1 (MQ=255)
                                                                                                                                                                                       ggCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAaagaa                                                                                       >  1:1786579/1‑289 (MQ=255)
                                                                                                                                                                                                  aGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACtt                                                                                              <  2:104472/271‑1 (MQ=255)
                                                                                                                                                                                                        ttCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAgcgc                                                                                                                                                                                            <  1:993937/171‑1 (MQ=255)
                                                                                                                                                                                                        ttCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGt                                                                      >  1:1259125/1‑289 (MQ=255)
                                                                                                                                                                                                        ttCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGt                                                                      >  1:421614/1‑289 (MQ=255)
                                                                                                                                                                                                         tCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACaa                                                                                                                                                                                                <  1:301899/166‑1 (MQ=255)
                                                                                                                                                                                                         tCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACaa                                                                                                                                                                                                >  2:301899/1‑166 (MQ=255)
                                                                                                                                                                                                               aaCTCAGACCAAACGGTTTTGCCGCTGCAAGCTGCAACGCTGACAGCGAAAAAGGCGGCGGCGCTGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAAt                                                               <  2:1786579/288‑1 (MQ=255)
                                                                                                                                                                                                                aCTCAGACCAAAACGTTTTGCCGGTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAAt                                                               <  2:591534/288‑1 (MQ=255)
                                                                                                                                                                                                                 cTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAaagaa                                                                                       >  1:1638455/1‑263 (MQ=255)
                                                                                                                                                                                                                     gACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCGCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGGCAGGAAGTTTGTAAActct                                                           >  2:967475/1‑287 (MQ=255)
                                                                                                                                                                                                                              ggtttACCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGAAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCgcggcg                                                <  2:510618/287‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGcacca                                           <  2:845410/289‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGcacca                                           >  2:1536782/1‑288 (MQ=255)
                                                                                                                                                                                                                                     cGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGcaccac                                          <  2:453170/288‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGcaccac                                          <  2:336023/288‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCTTCAATCGGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGAGTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGcaccac                                          <  2:1799855/288‑1 (MQ=255)
                                                                                                                                                                                                                                                    cgctgaaagcgaaaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCCCACCACCAGCCCAAGCACGGGc                          >  2:1564814/13‑289 (MQ=255)
                                                                                                                                                                                                                                                       tgagagcgagaaaGGCAGAGGCGCGGATTCTGAGTCCCGTGTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGTGGTTAACCACATGCGCCGCCAGTGGACGATGTAACAAGCGCCCTTCGTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTc                       <  2:1329835/279‑1 (MQ=255)
                                                                                                                                                                                                                                                         aaagcgaaaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGTCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCt                      >  1:31167/8‑288 (MQ=255)
                                                                                                                                                                                                                                                         aaagcgaaaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCa                                                                                                                >  1:352684/8‑198 (MQ=255)
                                                                                                                                                                                                                                                                aaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAGTAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGt              <  1:245202/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                aaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGt              <  1:870/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cgcggcgCGGATTCTGATGCCCGTTTATCGTTATGCCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCGCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACg        <  2:1726535/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ggcgCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACgg       <  2:1243694/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                     
CCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTTCTGGCAAATAGCGACAATCAGAACGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCACTAAGGCTAAAAGGCAGCGGCGCGGATTCACTTTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGT  >  CP042583/1527885‑1528441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A