Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,546,362 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,546,3620AT99.2% 583.0 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (101/145);  minor base G (0/2);  total (101/147)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,546,3630GC100.0% 581.4 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (101/147);  total (101/147)
*CP0425831,546,3640TA99.2% 586.4 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (99/147);  minor base C (2/0);  total (101/147)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GTGGTTGAGCAACAACACACCGTTCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAAAGTCATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAAAGTCATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGA  >  CP042583/1546082‑1546632
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                            
           accaaaaacCGTTGCAAAATGTGGTCGCGATTCGCGGTGGCGTGCCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACATGCCGTGGACGATGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac                                                                                                                                                                                                                                                             <  2:1555854/282‑1 (MQ=255)
                  acCGTTCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACCTGTGAAATCTATCTTCAATCCCATGGCGAATTTGAAaccacccc                                                                                                                                                                                                                                                       >  2:20052/1‑286 (MQ=255)
                   ccGTTCAAAAATTGCGTCGCTATTCGCGGTGGCGTACCTGTTTGCTTGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTTTTAGCGCATTTCCTCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                                                                                                                                                                                                                                     <  1:1079330/289‑1 (MQ=255)
                   ccGTTCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                                                                                                                                                                                                                                     <  1:1028218/289‑1 (MQ=255)
                   ccGTTCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                                                                                                                                                                                                                                     <  1:727550/289‑1 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGTCGAATTTGAAACCACCTCTGc                                                                                                                                                                                                                                                    >  1:878142/1‑287 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGACACCACCTCTGcc                                                                                                                                                                                                                                                   >  2:78240/1‑288 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac                                                                                                                                                                                                                                                             >  1:501515/1‑278 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGcc                                                                                                                                                                                                                                                   >  2:1845118/1‑288 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGc                                                                                                                                                                                                                                                    >  1:999282/1‑287 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGAGAAATCGATCTTGAATCCCATGGCGAATTTGAAACCAACCCTGcc                                                                                                                                                                                                                                                   >  2:734798/1‑288 (MQ=255)
                      ttCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGACTTTCCGCGTGGGTAGAACTTGTGAAATCGACCTTGAATCACATGGCGAATTTGAAACCACCTCTGcc                                                                                                                                                                                                                                                   >  2:362120/1‑288 (MQ=255)
                       tCAAAGATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGccc                                                                                                                                                                                                                                                  <  1:1115732/288‑1 (MQ=255)
                       tCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGc                                                                                                                                                                                                                                                    >  1:572663/1‑286 (MQ=255)
                       tCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTGGCTGGCCGTGGTGTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAAGTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGccc                                                                                                                                                                                                                                                  <  2:1396184/288‑1 (MQ=255)
                        cAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTg                                                                                                                                                                                                                                                <  1:621626/289‑1 (MQ=255)
                          gcacgggtgTCGCGATTCGCGGGGGGGTACCGGTTTGCTGGCCTTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                                                                                                                                                                                                                                               <  2:963840/280‑1 (MQ=255)
                           aaaTGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCAt                                                                                                                                                                                                                                             <  2:52972/289‑1 (MQ=255)
                             aTGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAc                                                                                                                                                                                                                                           <  1:1481466/289‑1 (MQ=255)
                             aTGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAc                                                                                                                                                                                                                                           <  1:1854218/289‑1 (MQ=255)
                             aTGGCCTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAc                                                                                                                                                                                                                                           <  2:514251/289‑1 (MQ=255)
                              tGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                                                                                                                                                                                                                                          <  2:808183/289‑1 (MQ=255)
                              tGGCGTCGCTATTCGCGGTGGCGTAACGGTTGTCTGGCCGTGGTTTGGTACGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGACGTGGATGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                                                                                                                                                                                                                                          <  2:1052620/289‑1 (MQ=255)
                                          tCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTAAtttctgtc                                                                                                                                                                                                                               >  1:963840/1‑283 (MQ=255)
                                                       ccGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTa                                                                                                                                                                                                                 <  2:571769/289‑1 (MQ=255)
                                                              gCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAg                                                                                                                                                                                                          <  2:831840/289‑1 (MQ=255)
                                                                  gCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTc                                                                                                                                                                                                      >  1:18110/1‑289 (MQ=255)
                                                                    cGTGGTTTGGTCCGGGGGAACAACAAGGTCGGCCTGCGCACGGTGTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGAACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCa                                                                                                                                                                                                     <  2:1622495/288‑1 (MQ=255)
                                                                    cGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAACTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGAGATATCGCTAAGGTAAGCGGCaa                                                                                                                                                                                                    >  2:1636801/1‑288 (MQ=255)
                                                                    cGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAAc                                                                                                                                                                                                                                 >  1:229334/1‑260 (MQ=255)
                                                                    cGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAAc                                                                                                                                                                                                                                 <  2:229334/260‑1 (MQ=255)
                                                                    cGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAg                                                                                                                                                                                                    <  1:734798/289‑1 (MQ=255)
                                                                     gTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGt                                                                                                                                                                                                   >  1:927043/1‑289 (MQ=255)
                                                                       ggTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGtgg                                                                                                                                                                                                 >  1:1503501/1‑289 (MQ=255)
                                                                            ggTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGtgggctgg                                                                                                                                                                                            >  1:1230585/1‑289 (MQ=255)
                                                                                 ggcggcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGAt                                                                                                                                                                                       <  2:1503501/289‑1 (MQ=255)
                                                                                  gcggcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTCACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATc                                                                                                                                                                                      >  2:1585385/1‑289 (MQ=255)
                                                                                  gcggcACAACAAGGCCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCt                                                                                                                                                                                                                  <  2:656604/261‑1 (MQ=255)
                                                                                  gcggcACAACAAGGCCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCt                                                                                                                                                                                                                  >  1:656604/1‑261 (MQ=255)
                                                                                                tCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATaaa                                                                                                                                                                        >  2:578895/1‑289 (MQ=255)
                                                                                                tCTGCCTGCGCACGGTTTTGCCCACAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTAGGCGATCGCTTCATTGATaaa                                                                                                                                                                        >  2:1713175/1‑289 (MQ=255)
                                                                                                   gcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTg                                                                                                                                                                     >  1:1622578/1‑289 (MQ=255)
                                                                                                   gcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTg                                                                                                                                                                     >  1:305312/1‑289 (MQ=255)
                                                                                                          cACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGAc                                                                                                                                                               >  2:1819248/1‑288 (MQ=255)
                                                                                                                   gccctcacacgaccGTGGTCGCTGAGATCGCATGATGAAGATACTGAGGGAGTAGCGCTGACTGTTGAAGTGATGCAAAGCGAAGAGAGGAAAAAATTCTGGCCGACTGAATGTACGCTGTTAGCGCATTTCATCGTGGGTACAACTTGTGAATGCGATCTTGAATCACATGGGGAACTTGAAGCCACATGTGCCCTGCTTACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATGGCTTCATTGATAAGGTGAACGACGCgaaagaaa                                                                                                                                                     <  2:1684597/277‑1 (MQ=255)
                                                                                                                       gCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGt                                                                                                                                                 >  1:1273902/1‑289 (MQ=255)
                                                                                                                       gCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATAGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGt                                                                                                                                                 >  1:702349/1‑289 (MQ=255)
                                                                                                                         gaCCTGACGTGGACGCTGAAAGCACATCATGAAGAGGCTGATGGCGTAGCCCTGACTGTTGAATTGACGCAAAGCGAGGAGACGAAAAAATGCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCGCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTa                                                                                                                                                <  2:1780762/287‑1 (MQ=255)
                                                                                                                         aaCCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTACCGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTAc                                                                                                                                               >  2:233615/1‑289 (MQ=255)
                                                                                                                         aaCCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGt                                                                                                                                                 >  1:1702923/1‑287 (MQ=255)
                                                                                                                          aCCTGCCGTGGACGCTGAAATCACATCATGAAGAAGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAATCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACt                                                                                                                                              <  2:30455/289‑1 (MQ=255)
                                                                                                                             tgcactGGACGATGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAGAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCGTTTCCGCGTGGGTAATACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTTGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGAc                                                                                                                                           <  2:477754/284‑1 (MQ=255)
                                                                                                                             tGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGAc                                                                                                                                           <  2:1192660/289‑1 (MQ=255)
                                                                                                                               ccGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGGCTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACTCGAAAGAAAATGTa                                                                                                                                                <  1:1525038/282‑1 (MQ=255)
                                                                                                                                 gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGAt                                                                                                                                       <  2:1139672/289‑1 (MQ=255)
                                                                                                                                 gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGAt                                                                                                                                       <  2:1910366/289‑1 (MQ=255)
                                                                                                                                   ggACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCa                                                                                                                                                                                <  1:1795528/246‑1 (MQ=255)
                                                                                                                                   ggACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg                                                                                                                                      <  1:1474572/288‑1 (MQ=255)
                                                                                                                                   ggACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg                                                                                                                                      <  1:330703/288‑1 (MQ=255)
                                                                                                                                   ggACGCTGAAATCAAATCAGTAAGATGCTGATGGCGTAGCGCGGACTTTTGAATTGACGCAAAGCGAAGAGCCGAAAAAAGTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGGATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg                                                                                                                                      <  2:1431646/288‑1 (MQ=255)
                                                                                                                                       gCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTAtt                                                                                                                                 <  1:1011132/289‑1 (MQ=255)
                                                                                                                                         tgaaatcacatcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCATAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACTACCTCTGCCCTGCATACCTACTTTAACGACGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGACCGCTTCATTGATAAAGTGAATGACGAGAAAGAAAATGTAATGACCGATGGttctc                                                                                                                                >  2:1525038/9‑284 (MQ=255)
                                                                                                                                         tgaaatcacatcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCa                                                                                                                               >  1:1145536/9‑289 (MQ=255)
                                                                                                                                         tgaaatcacatcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCa                                                                                                                               >  1:280023/9‑289 (MQ=255)
                                                                                                                                                  atcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTccc                                                                                                                      <  2:1222585/289‑1 (MQ=255)
                                                                                                                                                      tGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGAc                                                                                                                  <  2:1083844/289‑1 (MQ=255)
                                                                                                                                                            tGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACATGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACct                                                                                                                                                                                                                                                        <  1:646467/149‑1 (MQ=255)
                                                                                                                                                               tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGAt                                                                                                         <  1:347103/289‑1 (MQ=255)
                                                                                                                                                                gATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATc                                                                                                        <  2:1273902/289‑1 (MQ=255)
                                                                                                                                                                       tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGCAAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGtgtgca                                                                                                 >  2:913701/1‑287 (MQ=255)
                                                                                                                                                                       tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTa                                                                                                 >  1:1999759/1‑289 (MQ=255)
                                                                                                                                                                           gcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCt                                                                                              <  2:977099/288‑1 (MQ=255)
                                                                                                                                                                               aCTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCCAAGGTAAGCGTCAGTGGGCTGGGCGATCGATTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGACTGTATTCAGACCTTCCCTTACTGTACCTATccgcttgctacgaac                                                                                          >  2:1895274/1‑274 (MQ=255)
                                                                                                                                                                                 tattGCATTGACGCCAAGCGAAGGGACGAAAAAGTTATTGCCTGACGACTGTACTCTTTTAGCGAAGTTCCGCGTGGGTAAAACTTGTGAAATAGCTCTTGAATCACATGGCGAATTTGAACCCACCTCTTCCCTGCATACCTACTGTAGCGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCca                                                                                       <  2:1468028/287‑1 (MQ=255)
                                                                                                                                                                                    tGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACaa                                                                                    >  1:846188/1‑289 (MQ=255)
                                                                                                                                                                                    tGAATGGACGCAAAGCGAACAGACGAAAAAATTCTGGCATCACGACTCTACGCTGTTAGCTCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACaa                                                                                    <  2:79977/289‑1 (MQ=255)
                                                                                                                                                                                           aCGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg                                                                                                                                      <  2:1925855/232‑1 (MQ=255)
                                                                                                                                                                                           aCGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg                                                                                                                                      >  1:1925855/1‑232 (MQ=255)
                                                                                                                                                                                           aCGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCa                                                                             >  1:1909205/1‑289 (MQ=255)
                                                                                                                                                                                            cGCAAAGCGAAGAGTCTAAATAATTCTGGCCGCACGACTTTACGCTGTTATCGCATTTCCGGGTAGGTGAAACTTGTGGAATCTATCTTGAAGCACATGTCGAATTTGAACCCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTAGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCa                                                                             <  2:855301/288‑1 (MQ=255)
                                                                                                                                                                                                                    tCTGGCCGCACGACTTTACGCTGTTAGCGGATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGCCGAATTTTAAACCACCTCTTCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGaa                                                    <  2:434062/289‑1 (MQ=255)
                                                                                                                                                                                                                    tCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGaa                                                    >  1:106009/1‑289 (MQ=255)
                                                                                                                                                                                                                     cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAAt                                                   <  1:1174778/289‑1 (MQ=255)
                                                                                                                                                                                                                             aCGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGtattat                                           <  1:760564/289‑1 (MQ=255)
                                                                                                                                                                                                                             aCGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGtattat                                           <  1:1328904/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGCTGTTAGGGACTTTCCCCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAg                                   <  2:18110/289‑1 (MQ=255)
                                                                                                                                                                                                                                      cGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAgg                                  <  1:1836845/289‑1 (MQ=255)
                                                                                                                                                                                                                                         tGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATAGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGcca                               <  2:702349/289‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAACCCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGccac                              <  2:1702923/289‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTAGCGCATTTCCGCCTGGGTAAGACTTGTGAAATCGATCTTGACTCACATGGCGAATTTGAAACCCCCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGcca                               <  2:1356706/288‑1 (MQ=255)
                                                                                                                                                                                                                                           ttAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTa                                                                                                                                                >  2:1392934/1‑174 (MQ=255)
                                                                                                                                                                                                                                           ttAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTa                                                                                                                                                <  1:1392934/174‑1 (MQ=255)
                                                                                                                                                                                                                                           ttAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGAc                                                                                                                                           <  2:644051/179‑1 (MQ=255)
                                                                                                                                                                                                                                           ttAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGa                                                                                                                             <  1:1692094/193‑1 (MQ=255)
                                                                                                                                                                                                                                           ttAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGa                                                                                                                             >  2:1692094/1‑193 (MQ=255)
                                                                                                                                                                                                                                           ttAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGccacc                             >  2:1747306/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 cATTTCCGCGTGGGTAAAACGTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGcat                        <  2:280023/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            gggTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                                                                                                                                                                                                                                          <  1:1575965/67‑1 (MQ=255)
                                                                                                                                                                                                                                                              gTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTg           >  2:338818/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    cTTGTTAAATCGATCTTGAAGCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGATAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTg    <  2:1242524/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                            
GTGGTTGAGCAACAACACACCGTTCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAAAGTCATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAAAGTCATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGA  >  CP042583/1546082‑1546632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A