Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 473,068 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583473,0680GC99.4% 722.2 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (163/141);  total (164/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583473,0690CG99.4% 718.8 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (163/141);  total (164/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583473,0700TA99.3% 722.1 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (161/140);  minor base G (1/1);  total (164/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 306 total aligned reads displayed.

GTAATGTGATAACAAACCTTGTCCCCCGCGCATCGCTCGTTACCGCAACCGTGCCTTTATGCGCGACAACAATGCTTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTGCTAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGCACATCTTCCAGCTCCTTCTGGCTGC  >  CP042583/472788‑473348
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
gTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAg                                                                                                                                                                                                                                                                                  >  2:2330835/1‑289 (MQ=255)
          aaCAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCCCTCCGGTTGGCGTATAACGCAGGGCGGTCGAAAGCAGGTTGCTTAgc                                                                                                                                                                                                                                                                        >  1:1049413/1‑289 (MQ=255)
           aCAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAgc                                                                                                                                                                                                                                                                        >  2:449101/1‑288 (MQ=255)
             aaaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCGCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGcc                                                                                                                                                                                                                                                                     <  1:2623241/289‑1 (MQ=255)
                   tGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTTCACCAGGTGATCGACCGTCTGACAGGGCACTACAATTGTCTCTCCGGTTGGTGTATAACCCAGGGCGTCCGAAAGCAGGGTGCTTCACGCCCggac                                                                                                                                                                                                                                                                >  2:942047/1‑285 (MQ=255)
                   tGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACgc                                                                                                                                                                                                                                                               <  1:515505/289‑1 (MQ=255)
                          cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCt                                                                                                                                                                                                                                                                            <  1:1934305/269‑1 (MQ=255)
                          cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCt                                                                                                                                                                                                                                                                            >  2:1934305/1‑269 (MQ=255)
                          cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCa                                                                                                                                                                                                                                                        >  1:2257693/1‑289 (MQ=255)
                               atccgacGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTGACGCCCGACGCAGCAACCGCGGa                                                                                                                                                                                                                                                   >  2:1152018/7‑289 (MQ=255)
                               atccgacGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATc                                                                                                                                                                                                                                                         <  1:1761479/277‑1 (MQ=255)
                                     cGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGcc                                                                                                                                                                                                                                              <  1:147711/288‑1 (MQ=255)
                                      gTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCcg                                                                                                                                                                                                                                            <  1:2190548/289‑1 (MQ=255)
                                                   tGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACCATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTTTCTCTCCGGTTGGCGTATAACGAAGGGCGTTCGAAAGCCGGTTGATTAGCGCCCGACCCAGCATCAGCGGATCCCCCGCGACCTGACCCtt                                                                                                                                                                                                                               >  2:2135348/1‑289 (MQ=255)
                                                   tGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCcg                                                                                                                                                                                                                                            <  2:2205672/276‑1 (MQ=255)
                                                   tGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACtt                                                                                                                                                                                                                               >  1:903510/1‑289 (MQ=255)
                                                   tGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCCAc                                                                                                                                                                                                                                                                 >  2:1892865/1‑255 (MQ=255)
                                                         tatggccgACAACAATCGATTTGCCCATCGCCAGGCCAATACCGCAACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGGCAAACAAGCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGcc                                                                                                                                                                                                                         <  2:1683608/283‑1 (MQ=255)
                                                              gcgACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCcaaca                                                                                                                                                                                                                     <  1:449101/288‑1 (MQ=255)
                                                                  caacaaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAAcc                                                                                                                                                                                                                 >  1:1695734/1‑288 (MQ=255)
                                                                  caacaaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCg                                                                                                                                                                                                                <  1:2208962/289‑1 (MQ=255)
                                                                             ttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAAGAAACCGCAACCCCAcg                                                                                                                                                                                                      >  1:1196833/1‑288 (MQ=255)
                                                                               ccccaTCGCCACGCCAATACCGCTACCGTCGCCTTTTCGCGGGCTGGAGGGGTCAACGCGATAGGAACGGTCAACCAATCGCGGTTAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCAcgccg                                                                                                                                                                                                   <  2:644466/285‑1 (MQ=255)
                                                                                    tagccaagcagaTACCGCAACGTGAACCTGTTCGCTGGCGGGAGGGGACAACGCGATAGAAACGGTCAAACAATGCGGGTAAGTGCTCGGGCGCAATGGGGGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACAACAATTGTCTCTCCGGTGGGCGTATAACGCAGGTCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATc                                                                                                                                                                                              <  2:1641611/278‑1 (MQ=255)
                                                                                     cGCAAGGCCAATACCGCTCCCTTCACCTTTTCGAGGGCGGGATGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAAGGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCt                                                                                                                                                                                             <  2:1742595/289‑1 (MQ=255)
                                                                                      gCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCt                                                                                                                                                                                             >  1:1920093/1‑288 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGTGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGAGCCCGACGCAGCATCAGTGGATCGCCCGCGAACTGACACTTGTCGCCCACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACg                                                                                                                                                                                   >  1:1668652/1‑288 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGcc                                                                                                                                                                                                                                                                     <  1:125034/206‑1 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGcc                                                                                                                                                                                                                                                                     >  2:125034/1‑206 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACg                                                                                                                                                                                   >  1:838506/1‑288 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCCCTAACg                                                                                                                                                                                   >  2:989478/1‑288 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACCCCACGCCCCCATCTCCCCCTCAcc                                                                                                                                                                                   >  2:2661285/1‑287 (MQ=255)
                                                                                                tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGACAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACg                                                                                                                                                                                   >  1:1822705/1‑288 (MQ=255)
                                                                                                    gCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACCCCTGTCGCCAACAAACGGCAACCCCACGCCCCGATCTTCCGCCAAcccccc                                                                                                                                                                               >  2:1140544/1‑286 (MQ=255)
                                                                                                     cTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGa                                                                                                                                                                             >  1:1147343/1‑289 (MQ=255)
                                                                                                       aCCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGaaa                                                                                                                                                                           <  2:276672/289‑1 (MQ=255)
                                                                                                                    cGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACtt                                                                                                                                                              >  1:1011750/1‑289 (MQ=255)
                                                                                                                       tGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTg                                                                                                                                                            <  1:2041908/288‑1 (MQ=255)
                                                                                                                               ggggTCAACGCGAATGAAACGGTCAACCAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTc                                                                                                                                                    <  2:1852784/288‑1 (MQ=255)
                                                                                                                                    gaaCGCGATAGAAACGGTCAAGCAGTCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGc                                                                                                                                              <  2:876080/288‑1 (MQ=255)
                                                                                                                                     aaCGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCCCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTCCCGACTTCATCCGc                                                                                                                                              >  2:1338127/1‑288 (MQ=255)
                                                                                                                                          gATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGtt                                                                                                                                        >  1:2563359/1‑289 (MQ=255)
                                                                                                                                           aTAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATTGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGa                                                                                                                                                                                                                                                   >  2:2306411/1‑181 (MQ=255)
                                                                                                                                           aTAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATTGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGa                                                                                                                                                                                                                                                   <  1:2306411/181‑1 (MQ=255)
                                                                                                                                                    gTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCCAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCCTCCGCCCGGTTGAGCCttt                                                                                                                                >  2:1554639/1‑287 (MQ=255)
                                                                                                                                                              cgcgGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGa                                                                                                                    <  1:529300/289‑1 (MQ=255)
                                                                                                                                                              cgcgGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGa                                                                                                                    <  2:846359/289‑1 (MQ=255)
                                                                                                                                                                   tAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAg                                                                                                                <  1:2715644/288‑1 (MQ=255)
                                                                                                                                                                     aGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTg                                                                                                             >  2:541177/1‑289 (MQ=255)
                                                                                                                                                                           cGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTa                                                                                                                                                                                                                                                                          >  1:571197/1‑126 (MQ=255)
                                                                                                                                                                           cGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTa                                                                                                                                                                                                                                                                          <  2:571197/126‑1 (MQ=255)
                                                                                                                                                                             ggCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCcg                                                                                                                                                                                                                                            <  1:971348/154‑1 (MQ=255)
                                                                                                                                                                                 cAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGGTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCgg                                                                                                  >  2:2263407/1‑288 (MQ=255)
                                                                                                                                                                                  aaTGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCcg                                                                                                                                                                                                                                            <  2:1046686/149‑1 (MQ=255)
                                                                                                                                                                                   aTGGGCGTACCGGGGTTTTCGCCGATAACATGCACCAGGGGATCGACCGTCTGACATCGCACTACAATTGTCTCTCCTGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCt                                                                                               <  2:1991637/289‑1 (MQ=255)
                                                                                                                                                                                    tGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCACCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTg                                                                                              >  1:1489324/1‑289 (MQ=255)
                                                                                                                                                                                       gCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAg                                                                                            <  2:2567434/288‑1 (MQ=255)
                                                                                                                                                                                        cGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCg                                                                                          >  2:1516067/1‑289 (MQ=255)
                                                                                                                                                                                            ccGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACgcagc                                                                                                                                                                                                                                                            <  1:319693/123‑1 (MQ=255)
                                                                                                                                                                                            ccGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCgg                                                                                                                       >  1:1591151/1‑256 (MQ=255)
                                                                                                                                                                                            ccGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCgg                                                                                                                       <  2:1591151/256‑1 (MQ=255)
                                                                                                                                                                                             cGGGGTTTGCGACGAGAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACGACGATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGaaa                                                                                     <  1:287087/289‑1 (MQ=255)
                                                                                                                                                                                                ggTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCa                                                                                                                                                                                                              <  2:2550836/165‑1 (MQ=255)
                                                                                                                                                                                                ggTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACa                                                                                   >  1:1421861/1‑288 (MQ=255)
                                                                                                                                                                                                            tAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTAt                                                                                                     <  1:605187/258‑1 (MQ=255)
                                                                                                                                                                                                                   cACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCg                                                                                                                                                                     <  1:2678497/187‑1 (MQ=255)
                                                                                                                                                                                                                   cACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGAACAtttt                                                                >  1:2025650/1‑288 (MQ=255)
                                                                                                                                                                                                                    aCCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCAACCGCCAGGGTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAAAAGCATATCGCTGACCATTTTc                                                               >  1:1733588/1‑288 (MQ=255)
                                                                                                                                                                                                                             tCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGAACGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGACAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTGCCTGGTTGTTACCGGCCTTAGCGAGAAACAGCATATAGCTCTCCATTTTCGCCATTcgcg                                                     >  1:8147/1‑289 (MQ=255)
                                                                                                                                                                                                                              cGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTcgcg                                                     >  2:1483377/1‑288 (MQ=255)
                                                                                                                                                                                                                                 ccGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAg                                                 <  2:1822705/289‑1 (MQ=255)
                                                                                                                                                                                                                                    tCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGa                                                                                                                                      >  2:2087179/1‑201 (MQ=255)
                                                                                                                                                                                                                                    tCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGa                                                                                                                                      <  1:2087179/201‑1 (MQ=255)
                                                                                                                                                                                                                                    tCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGTCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGc                                                >  1:720834/1‑287 (MQ=255)
                                                                                                                                                                                                                                    tCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGc                                                >  2:755919/1‑287 (MQ=255)
                                                                                                                                                                                                                                    tCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATACCGCTGACCATTTTCGCCATTCGCCTCCGc                                                >  2:1004969/1‑287 (MQ=255)
                                                                                                                                                                                                                                       gACAGCGCACTACAATTGGCTTGCCGGTGGGCGAAAAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTc                                           <  2:2345783/289‑1 (MQ=255)
                                                                                                                                                                                                                                        aCAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTc                                           <  2:523308/288‑1 (MQ=255)
                                                                                                                                                                                                                                        aCAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTc                                           <  2:301403/288‑1 (MQ=255)
                                                                                                                                                                                                                                         ctgcgacatACATTTGGCTAGCCGGTTGGCGGATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAGACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCga                                         <  2:324788/281‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAac                                                                                                                                                                  >  2:1084839/1‑165 (MQ=255)
                                                                                                                                                                                                                                            cgcACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATAAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCAGCTAACGCCTCGAAAAAATCGAaca                                                                                                                                                                 <  2:2491594/166‑1 (MQ=255)
                                                                                                                                                                                                                                              cACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATta                                    >  1:2469802/1‑289 (MQ=255)
                                                                                                                                                                                                                                              cACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATta                                    >  1:1889195/1‑289 (MQ=255)
                                                                                                                                                                                                                                               aCTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATta                                    <  2:2691633/288‑1 (MQ=255)
                                                                                                                                                                                                                                               aCTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATta                                    <  1:2333745/288‑1 (MQ=255)
                                                                                                                                                                                                                                                  aCAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATtagagt                                >  1:784767/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      ttGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTg                                                                                                                                                            <  1:1135960/161‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGAACATTTTCGCCATTCGCGTCAGCTCTTCGAGATtagagtaga                             >  2:1402100/1‑288 (MQ=255)
                                                                                                                                                                                                                                                         tctctcCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGcac                         >  1:2131734/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          ctttcCGGTGGGCGAATAGGTCATGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGCAGCGCCCGCGACCTGACCCTGGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTGTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGcaca                        <  2:1196833/286‑1 (MQ=255)
                                                                                                                                                                                                                                                          ctctcCGGTTGGCGAATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGcac                         <  1:1362736/288‑1 (MQ=255)
                                                                                                                                                                                                                                                           tctcCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGcaca                        >  1:1524139/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           tctcCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGcaca                        >  1:2287584/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           tctcCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGcaca                        >  1:2381903/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             tcCGGTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCcaa                                                                                                                                                                                                                       >  1:953394/1‑95 (MQ=255)
                                                                                                                                                                                                                                                                 gTTGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATta                                    <  2:968435/270‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tGGCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGCACATCTTCCAg                <  1:857747/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTTATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGCCTAGTTTCCAGCt              >  2:1454034/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                     gCGTATAACGCAGGGCGTTCGAAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGCACATCTTCCAGCt              >  1:1855179/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
GTAATGTGATAACAAACCTTGTCCCCCGCGCATCGCTCGTTACCGCAACCGTGCCTTTATGCGCGACAACAATGCTTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTGCTAAGCAGGTTGCTTAGCGCCCGACGCAGCATCAGCGGATCGCCCGCGACCTGACACTTGTCGCCAACAAACCGCAACTCCACGCCGCGATCTTCCGCTAACGCCTCGAAAAAATCGAACACTTTGCCGACTTCATCCGCCAGGTTGAGCATTTTCTTTTCGGGGATTAGCTGGTTGTTATCGGCCTGAGCGAGAAACAGCATATCGCTGACCATTTTCGCCATTCGCGTCAGCTCTTCGAGATTAGAGTAGAGCACATCTTCCAGCTCCTTCTGGCTGC  >  CP042583/472788‑473348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A