Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,653,330 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,653,3300GC99.7% 789.4 / NA 333intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (158/173);  total (158/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,653,3310CG99.7% 784.0 / NA 333intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (157/174);  total (158/175)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,653,3320TA99.7% 799.3 / NA 333intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (157/175);  minor base C (1/0);  total (158/175)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

AACGAACTGGCTCATGCTGCAATGACCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAAAGCATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑GCTAAAACCGTCTTTGCTAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCGCTGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCACTTTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTA  >  CP042583/1653053‑1653609
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                     
    aaCTGGCTCATGCTGCAATGACCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAAttt                                                                                                                                                                                                                                                                           >  1:796775/1‑288 (MQ=255)
                   cAATGACCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACAGTTCAAATCTATTATTGATAAATTAAGCCTGGCATGCAAAAGCGGCGAAGGTCTTTATTATAACGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGAGGCAGGTTTACTGCCCCCGTTTCAAATCCGCGACGGCAAATCTTCCACTGAGCGCTCGGTAAAA‑CGTCTTTCGAAGGTATAAGTTTGCCTTTTGCTGCCAGa                                                                                                                                                                                                                                                           >  2:1599196/1‑289 (MQ=255)
                        aCCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATACATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGc                                                                                                                                                                                                                                                      >  2:1261559/1‑289 (MQ=255)
                        aCCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAAc                                                                                                                                                                                                                                                        >  2:2301879/1‑287 (MQ=255)
                        aCCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACg                                                                                                                                                                                                                                                       >  2:1221179/1‑288 (MQ=255)
                              cAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAg                                                                                                                                                                                                                                                <  1:1183557/289‑1 (MQ=255)
                                      cccAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTccc                                                                                                                                                                                                                                         >  2:737412/1‑288 (MQ=255)
                                       ccAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACGCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCTTTTTGCTGCCAGCAACGCTACCAGCTCTccc                                                                                                                                                                                                                                         >  2:450459/1‑287 (MQ=255)
                                                          aaTGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTgccgag                                                                                                                                                                                                                    <  1:128568/289‑5 (MQ=255)
                                                                 tGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGgtgt                                                                                                                                                                                                             >  2:219728/1‑289 (MQ=255)
                                                                         agcagcATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCTTTTTGCTGCCAGAAACGCTACCAGCTCTCCCTCTGTCATCCCTTCGTCCGACCAGGTGTGAAAACGt                                                                                                                                                                                                     >  2:2288552/1‑289 (MQ=255)
                                                                         agcagcATGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTAcc                                                                                                                                                                                                                                                  <  1:1589604/244‑1 (MQ=255)
                                                                              caTGAAGAAGAGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGCGCAGGTGTGAAAACGTg                                                                                                                                                                                                    >  1:1971562/1‑285 (MQ=255)
                                                                                   agaagaGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTAc                                                                                                                                                                                                                                                   <  2:2711319/233‑1 (MQ=255)
                                                                                   agaagaGAAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCAGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCa                                                                                                                                                                                            >  1:1389461/1‑288 (MQ=255)
                                                                                        agaAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCaaa                                                                                                                                                                                       >  1:372593/1‑288 (MQ=255)
                                                                                        agaAACTGTTCAAATCGATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCaaa                                                                                                                                                                                       >  1:1235753/1‑288 (MQ=255)
                                                                                                     atcgattattGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATc                                                                                                                                                                          >  1:557588/5‑288 (MQ=255)
                                                                                                       cgattataGATAAATTACGCCTGGCAGGCAAAAGCAGGGAAGATCTGTATTTTATAGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCa                                                                                                                                                                       <  2:1389461/287‑1 (MQ=255)
                                                                                                          ttattGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGc                                                                                                                                                                        >  2:2073504/1‑285 (MQ=255)
                                                                                                           tattGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCtt                                                                                                                                                                   <  1:2203167/289‑1 (MQ=255)
                                                                                                                aTAAATTAAACCTGGCAGGCAAAAGGCGCGAAGGTCTGTATTTTATCCACAAAGAACTCTCTACCCCCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGa                                                                                                                                                              <  2:1457624/289‑1 (MQ=255)
                                                                                                                 tAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGa                                                                                                                                                              <  2:674822/288‑1 (MQ=255)
                                                                                                                    aTTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          <  1:2170593/289‑1 (MQ=255)
                                                                                                                    aTTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          <  1:566531/289‑1 (MQ=255)
                                                                                                                    aTTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          <  1:750317/289‑1 (MQ=255)
                                                                                                                    aTTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          <  2:621833/289‑1 (MQ=255)
                                                                                                                      tAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCa                                                                                                                                                        <  2:173707/289‑1 (MQ=255)
                                                                                                                       aaGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCAt                                                                                                                                                       <  1:17588/289‑1 (MQ=255)
                                                                                                                              gcaggcaAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTcc                                                                                                                                                                                                                                          <  1:1844155/199‑1 (MQ=255)
                                                                                                                              gcaggcaAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTcc                                                                                                                                                                                                                                          >  2:1844155/1‑199 (MQ=255)
                                                                                                                              gcaggcaAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGtata                                                                                                                                                >  2:2446907/1‑289 (MQ=255)
                                                                                                                                  gcaAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTg                                                                                                                                             >  1:1453574/1‑288 (MQ=255)
                                                                                                                                   caAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTg                                                                                                                                             <  2:1078669/287‑1 (MQ=255)
                                                                                                                                    acaaGCGGCGAAGGTCTGTATTTTATCGACAAACAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTc                                                                                                                                          <  1:2097017/287‑1 (MQ=255)
                                                                                                                                    aaaaGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTc                                                                                                                                          >  1:2462383/1‑289 (MQ=255)
                                                                                                                                          ggcgAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGcctg                                                                                                                                     <  1:2288552/288‑3 (MQ=255)
                                                                                                                                          ggcgAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGcct                                                                                                                                      <  1:2402267/287‑2 (MQ=255)
                                                                                                                                               ctgTCTATATTCTATCGACAAAGCACTCACTCCGCCCTACACCCAAAACTAATGCTCTCCGGCGGCAGGTTTACGGCCGCCATTTCAATTCAGGGCCGTAAAATCTTACGCTGATCGGT‑CAAAAACCCGTCTTTGTAAGGTATCAATTTCCCTTTTGCCGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCATGTGTGAAAACGTGCCTGTTAACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGcctgattcaa                                                                                                                               <  2:1333523/287‑9 (MQ=255)
                                                                                                                                                  tCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAatca                                                                                                                            <  2:2122637/289‑1 (MQ=255)
                                                                                                                                                           tatagcaaaaacgctctcgACCCTCGACACAGAAACCTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCGGATCTGT‑‑CGAAACCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCGCCCGCTGTAATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  <  2:141570/276‑1 (MQ=255)
                                                                                                                                                           tATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGc                                                                                                                                                                                                   >  2:2368188/1‑209 (MQ=255)
                                                                                                                                                           tATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGa                                                                                                                                                              >  1:265693/1‑246 (MQ=255)
                                                                                                                                                           tATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCCGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          <  2:1576564/250‑1 (MQ=255)
                                                                                                                                                           tATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCCGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          >  1:1576564/1‑250 (MQ=255)
                                                                                                                                                           tATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCATTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGc                                                                                                                                                                                                   <  1:1914270/209‑1 (MQ=255)
                                                                                                                                                           tATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCATTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGc                                                                                                                                                                                                   >  2:1914270/1‑209 (MQ=255)
                                                                                                                                                            aTCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGTCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  <  1:2701620/289‑1 (MQ=255)
                                                                                                                                                            aTCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCt                                                                                                                                                                                                                                      >  2:779840/1‑173 (MQ=255)
                                                                                                                                                            aTCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCt                                                                                                                                                                                                                                      <  1:779840/173‑1 (MQ=255)
                                                                                                                                                            aTCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  <  1:967180/289‑1 (MQ=255)
                                                                                                                                                            aTCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  <  2:2039944/289‑1 (MQ=255)
                                                                                                                                                                   aaGCACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCa                                                                                                           <  1:2052086/289‑1 (MQ=255)
                                                                                                                                                                   aaGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCa                                                                                                           <  1:2251152/289‑1 (MQ=255)
                                                                                                                                                                   aaGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCa                                                                                                           <  1:344097/289‑1 (MQ=255)
                                                                                                                                                                   aaGAACTCTCTACCCTCGACACACAAAACTAATGCTCCCGGGCGGCAGGTTTACTGCCGCCGTTGAAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAGGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCa                                                                                                           <  2:310201/289‑1 (MQ=255)
                                                                                                                                                                       gctgtctACCCTCGACACACAAAACTAATGCTCGCCGGCGGGAGGTTTACTGCCGCCTTTTCACATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATATATTTTCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCa                                                                                                           <  2:372352/281‑1 (MQ=255)
                                                                                                                                                                       aCTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATg                                                                                                         <  1:2558154/287‑1 (MQ=255)
                                                                                                                                                                           tctACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTg                                                                                                   >  2:896131/1‑289 (MQ=255)
                                                                                                                                                                           tctACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCCCGCCTGATTCAATCATCATATTTAACACTTCCTGACCGta                                                                                                   >  2:32948/1‑288 (MQ=255)
                                                                                                                                                                               cccACGACACACAACAATAATGCTCTCCGGCGGCATGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAATGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATa                                                                                               <  2:2309390/289‑1 (MQ=255)
                                                                                                                                                                                ccTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTTGCATGCGTATATTg                                                                                                                                             >  2:544651/1‑242 (MQ=255)
                                                                                                                                                                                 cTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATaga                                                                                             <  2:930630/289‑1 (MQ=255)
                                                                                                                                                                                    gACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAACTCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCCCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTTCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGAATGGCATGCGTACATTGCCCGCCTGATTCAATCATCATATTTAACACTTCATAACCGGGAATaacgt                                                                                           >  2:568467/1‑284 (MQ=255)
                                                                                                                                                                                    gACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCACCTTCCAGACTGGCCTGCGTATATTGCACGCCTGATTCAATCATCATACTTAACACTTCTTGACAGTGAACagactt                                                                                          >  2:2609371/1‑286 (MQ=255)
                                                                                                                                                                                     acacacAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTgccgag                                                                                                                                                                                                                    <  1:656039/166‑5 (MQ=255)
                                                                                                                                                                                         acaAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGta                                                                                                                                                  <  2:2659925/228‑1 (MQ=255)
                                                                                                                                                                                          caAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTgg                                                                                                                                                          >  2:398409/1‑219 (MQ=255)
                                                                                                                                                                                                  aTGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  <  1:1102924/251‑1 (MQ=255)
                                                                                                                                                                                                  aTGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  >  2:1102924/1‑251 (MQ=255)
                                                                                                                                                                                                  aTGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCctcctc                                                                            >  1:2357265/1‑289 (MQ=255)
                                                                                                                                                                                                   tGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAatca                                                                                                                            <  1:901506/240‑1 (MQ=255)
                                                                                                                                                                                                       ctcCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGAcc                                                                                                      <  2:2017297/258‑1 (MQ=255)
                                                                                                                                                                                                        tcCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAAcg                                                                                                                                                                                    <  1:1004429/179‑1 (MQ=255)
                                                                                                                                                                                                        tcCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCCTATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCaaaaaa                                                                      >  1:497208/1‑289 (MQ=255)
                                                                                                                                                                                                        tcCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCaaaaaa                                                                      >  2:2589149/1‑289 (MQ=255)
                                                                                                                                                                                                         ccGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGcctgattcaa                                                                                                                               <  1:2615989/231‑9 (MQ=255)
                                                                                                                                                                                                         ccGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCaaaaaaa                                                                     >  1:446740/1‑289 (MQ=255)
                                                                                                                                                                                                                cAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAatca                                                                                                                            <  1:302617/227‑1 (MQ=255)
                                                                                                                                                                                                                            cgccgTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGc                                                   <  1:709866/288‑1 (MQ=255)
                                                                                                                                                                                                                             gccgTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGcc                                                  >  1:2082785/1‑288 (MQ=255)
                                                                                                                                                                                                                             gccgTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCg                                                 <  2:2227001/289‑1 (MQ=255)
                                                                                                                                                                                                                             gccgTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCg                                                 <  2:2428433/289‑1 (MQ=255)
                                                                                                                                                                                                                              ccgTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtatcgtagcgtattccg                                                 >  2:2483363/1‑281 (MQ=255)
                                                                                                                                                                                                                                               cGGCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTgcgc                               <  1:1361064/289‑1 (MQ=255)
                                                                                                                                                                                                                                               cGGCAAATCTTACCTGGTTCGGT‑CTAAAAACCGGCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTgcgc                               <  2:1453574/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCAAATCTTACTCTGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCa                             >  1:1394/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    aaTCTTACTCTGATCGGT‑CGAAAAACCGACTTTCGACGGTATAAATTTGCCTTTTGCGGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATGGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAgcg                          <  2:763795/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        ttACTCTGATCGGT‑CGAAACACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGATCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCa                        <  1:244044/287‑1 (MQ=255)
                                                                                                                                                                                                                                                           ctctGATCGGT‑CGAAAAACCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTaa                                                                                                                  <  1:2303415/194‑1 (MQ=255)
                                                                                                                                                                                                                                                                 atcggtcgaaaaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTTCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTGACTAAGACTTTGCGCCAGCGCCGGAcgtaat               >  2:2697142/10‑283 (MQ=255)
                                                                                                                                                                                                                                                                 atcggtcgaaaaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAatcatc                                                                                                                          >  2:816022/10‑181 (MQ=255)
                                                                                                                                                                                                                                                                 atcggtcgaaaaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGcc                                                                  >  1:201979/10‑237 (MQ=255)
                                                                                                                                                                                                                                                                 atcggtcgaaaaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAAt               >  1:269050/10‑288 (MQ=255)
                                                                                                                                                                                                                                                                 atcggtcgaaaaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAAt               >  1:1212661/10‑288 (MQ=255)
                                                                                                                                                                                                                                                                 atcggtcgaaaaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACGGAAt               >  1:177564/10‑288 (MQ=255)
                                                                                                                                                                                                                                                                           aaaCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTg    <  1:1647314/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aaaCCGGCTTTCGAAAGTATAAATTTGCCTTTTGCTGCCAGAAACCCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTTCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTg    <  2:177564/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aCCGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACAAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTa  <  2:1152336/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccGTCTTTCGAAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCa                                                                                                           <  2:1371800/183‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                     
AACGAACTGGCTCATGCTGCAATGACCAATCAGGACTACCCAACATTTAATTTCCTGCAATGGTATGTTTCTGAGCAGCATGAAGAAGAGAAACTGTTCAAAAGCATTATTGATAAATTAAGCCTGGCAGGCAAAAGCGGCGAAGGTCTGTATTTTATCGACAAAGAACTCTCTACCCTCGACACACAAAACTAATGCTCTCCGGCGGCAGGTTTACTGCCGCCGTTTCAAATCAGTGACGGCAAATCTTACTCTGATCGGT‑GCTAAAACCGTCTTTGCTAGGTATAAATTTGCCTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCGCTGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCACTTTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTA  >  CP042583/1653053‑1653609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A