Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,668,563 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,668,5630GC99.3% 634.4 / NA 270intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (121/146);  total (122/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,668,5640CG98.9% 630.9 / NA 270intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (120/147);  total (122/148)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,668,5650TA99.2% 628.8 / NA 270intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (119/147);  minor base C (2/0);  total (122/148)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 270 total aligned reads displayed.

CAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCTTAGTTATCGACTACGTCGGCCATGTTGTGCCTCTTGCTGACGCA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACGCTGTTACCGAACTGGCGATAGGCCTGAGTGTCGCTAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGATGTTTTTTCGCATATCGAT  >  CP042583/1668287‑1668843
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                       
aaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATa                                                                                                                                                                                                                                                                               <  1:1108345‑M2/114‑1 (MQ=255)
aaatccggacgtccgggcagactggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATa                                                                                                                                                                                                                                                                               <  1:889045‑M2/114‑1 (MQ=255)
                      tggcgtcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGgc                                                                                                                                                                                                                                                          >  1:1913995‑M2/154‑288 (MQ=255)
                      ggactgcatcaccccgaaatgccgagccctgcccggtgaacaggctggcgaggcgcttcgctagcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGgcg                                                                                                                                                                                                                                                         <  2:755328‑M2/136‑1 (MQ=255)
                           tcctgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCCAGCAGTTTTGCCACCGCGGCAAAGACCGGCCCGACCACCGAGTTACCGAACTGGCCGTAGGCCTGAGTGTCCGCAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCa                                                                                                                                                                                                                                                    >  2:1845948‑M2/149‑289 (MQ=255)
                              tgaacgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaaaa                                                                                                                                                                                                                                                 <  1:2567319‑M2/144‑1 (MQ=255)
                                  cgcgaaatgccaggccctgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTTGCTTCTCCCGGCGCTTCAAAACCCa                                                                                                                                                                                                                                             >  1:354415‑M2/142‑289 (MQ=255)
                                         tgccaggccctgcccggttcacaaactggcgaggcgcttcgctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAgcg                                                                                                                                                                                                                                      <  2:1128302‑M2/155‑1 (MQ=255)
                                             tgccccggcccggttaacgggctggcggtgagcttcgctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccattttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAgcgcgcg                                                                                                                                                                                                                                  <  1:650600‑M2/159‑1 (MQ=255)
                                                ccctgcccggttaacagactggggaggcgcttctctatcgcggtttcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgccgcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAgcgcgcg                                                                                                                                                                                                                                  <  2:1774596‑M2/159‑1 (MQ=255)
                                                   tgcccggttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACTGAATTTCGCTTCCTCCCGCGCTTCAAAACCCAGTAAGCGGCCCCATTc                                                                                                                                                                                                                             >  1:1259043‑M2/125‑288 (MQ=255)
                                                     gccggttaagcgactggcgcggcgcttctttatcgccgtataacgcgtggcaatcgcgcgattatttttgctgcgggtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCg                                                                                                                                                                                                                          <  2:2539583‑M2/167‑1 (MQ=255)
                                                      caggttagcagcctggcggagcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCg                                                                                                                                                                                                                          <  1:2464566‑M2/167‑1 (MQ=255)
                                                         gttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGc                                                                                                                                                                                                                      >  1:1164889‑M2/119‑289 (MQ=255)
                                                          ttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCgg                                                                                                                                                                                                                                                           <  1:946572‑M2/134‑1 (MQ=255)
                                                          ttaacagactggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCgg                                                                                                                                                                                                                                                           >  2:946572‑M2/118‑251 (MQ=255)
                                                                   tggcggggcgattctctatcgccgtatcacgcgtgggaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGAc                                                                                                                                                                                                            <  2:110077‑M2/181‑1 (MQ=255)
                                                                   tggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGCATACGGAATTTCGCGTCTCCCGGCGCATCACAACCCATTAAGCGCGCGCATTCCCGAGGCTCTAACcgc                                                                                                                                                                                                             >  2:1075757‑M2/109‑287 (MQ=255)
                                                                    ggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCCGAATACGGAATTTCGCTTCCCCCGGCGCTGCAAAACCCCTTAACCCCCCCCATTCCCCAGGCGttaacccccc                                                                                                                                                                                                           >  2:695366‑M2/108‑285 (MQ=255)
                                                                    ggcgaggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACT‑CCCCCCCCCCCCCCCCCCCCCCCCCCCACTACCGACTTTCGCTTCTCCCGGCGCTTCAACACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGt                                                                                                                                                                                                          >  1:1606420‑M2/108‑289 (MQ=255)
                                                                         ggggctgctctaccgccgtatgcgtcgtggaaatcgcgtggatatttttgctgcgggtggagttatcgtgcacgtctgccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCGGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGGCCGCCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGa                                                                                                                                                                                                       <  2:1428999‑M2/186‑1 (MQ=255)
                                                                         ggcgcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGa                                                                                                                                                                                                       <  1:40405‑M2/186‑1 (MQ=255)
                                                                            gcttctctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGAt                                                                                                                                                                                                   <  2:2017454‑M2/190‑1 (MQ=255)
                                                                                 tctatcgccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGc                                                                                                                                                                                              <  1:1192823‑M2/195‑1 (MQ=255)
                                                                                       gccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCCCTTCAAAACCCATGAAGCGCGCGCATTCCCGAGGCGGTTACCGACCGGGACGATGTTGACGATTc                                                                                                                                                                                        >  2:1056417‑M2/89‑289 (MQ=255)
                                                                                        ccgtatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGaa                                                                                                                                                                                                                                                                          <  2:1560923‑M2/119‑1 (MQ=255)
                                                                                           tatcacgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGGCCGAAACCGGAATACGGAATTTCGCTTCCCCCGGCGCTTCAAAACCCATTAAGCGCGCGCCTTCCCCAGGCGTTAACCGACGTTGACGATGTTTCTGATTCAGCg                                                                                                                                                                                    >  2:488340‑M2/85‑289 (MQ=255)
                                                                                               acgcgtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATc                                                                                                                                                                                >  1:1399947‑M2/81‑289 (MQ=255)
                                                                                                   gtggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCa                                                                                                                                                                           >  1:1540743‑M2/77‑289 (MQ=255)
                                                                                                    tggcaatcgagcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCAACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCa                                                                                                                                                                           <  1:1659289‑M2/213‑1 (MQ=255)
                                                                                                     ggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCaaa                                                                                                                                                                         >  1:1871303‑M2/75‑289 (MQ=255)
                                                                                                     ggcaatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGCGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGAc                                                                                                                                                                                                            >  2:310607‑M2/75‑255 (MQ=255)
                                                                                                      gcaatgctgtgaatatttttgctgcgagtggccttatcggggacggcggccatgtagtgccgcttgctgacgcACACGCCA‑CGCCGGGTTGATTTTTGGCTCAAGCGGTTTTGCCACCGAGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCaaa                                                                                                                                                                         <  2:1623197‑M2/215‑1 (MQ=255)
                                                                                                       caatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCCACGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCACAACCCATGAAGCGCGCGCATTCCCGAGTCGTAAACCTACGTGGACTATGCTCCTTATTCAGCGGATCG‑tcaact                                                                                                                                                                        >  2:2342037‑M2/73‑286 (MQ=255)
                                                                                                       caatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAg                                                                                                                                                                        <  1:1834068‑M2/216‑1 (MQ=255)
                                                                                                       caatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAg                                                                                                                                                                        <  1:2185742‑M2/216‑1 (MQ=255)
                                                                                                       caatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAg                                                                                                                                                                        >  1:570836‑M2/73‑288 (MQ=255)
                                                                                                       caatcgcgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAg                                                                                                                                                                        <  1:1593199‑M2/216‑1 (MQ=255)
                                                                                                             cgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTtc                                                                                                                                                                  >  1:2412221‑M2/67‑288 (MQ=255)
                                                                                                             cgcgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCAATAAGCGCGCGCATTCACGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTtc                                                                                                                                                                  >  1:2718531‑M2/67‑288 (MQ=255)
                                                                                                               cgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCaaaa                                                                                                                                                                                                                                                 <  1:225506‑M2/144‑1 (MQ=255)
                                                                                                               cgcatatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTtctc                                                                                                                                                                >  1:857428‑M2/65‑288 (MQ=255)
                                                                                                                  atatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCAccc                                                                                                                                                            <  1:2390777‑M2/228‑1 (MQ=255)
                                                                                                                   tatttttgctgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCg                                                                                                                                                           <  1:1144505‑M2/229‑1 (MQ=255)
                                                                                                                            tgcgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTCGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGAGGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGACTTTCTCCACCGTGGCCatat                                                                                                                                                  >  1:2494658‑M2/52‑289 (MQ=255)
                                                                                                                              cgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTtctc                                                                                                                                                                >  1:60661‑M2/50‑273 (MQ=255)
                                                                                                                              cgagtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATc                                                                                                                                                 <  1:300026‑M2/239‑1 (MQ=255)
                                                                                                                                agtggccttatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCg                                                                                                                                                           >  2:666138‑M2/48‑276 (MQ=255)
                                                                                                                                        tatcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAACCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGACTTTGCTGATTCAGCGGAACG‑TCAAAGGCTTTCTCACCCGTGGCCATATCCCAGccgcgc                                                                                                                                       >  2:1834869‑M2/40‑287 (MQ=255)
                                                                                                                                         atcgtgaacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGCACTGGCGATAGGCCTGAGTGTCCGAACCCGGCCTACGGAATTTCGCTCCTCCCGCCCCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGCACGATGTTGCTCATTCCGCGGAACG‑CCGAACTCTTTCGCCCCCGTGCCCGTCTCCCagccgcgtcc                                                                                                                                     >  2:1201873‑M2/39‑286 (MQ=255)
                                                                                                                                               aacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCAccc                                                                                                                                                            <  2:1875259‑M2/228‑1 (MQ=255)
                                                                                                                                               aacgtcggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATAACCCAGCCGCGATCGATTaa                                                                                                                               >  1:2612148‑M2/33‑289 (MQ=255)
                                                                                                                                                agggaggccaggttgtggctttgggtgcggaA‑ACGCCAGCGCGTGTTTGATTTTTGGCTCGAGCAGTTTGGCGACGGCGGCAAAGCCCGGGACGACCACGGAGTGACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTaaa                                                                                                                              <  2:2686233‑M2/258‑1 (MQ=255)
                                                                                                                                                    cggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCCAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCCTCCACGGGGCCAGATCCCCGCCGCGATCGATTAAAAttt                                                                                                                          >  2:127457‑M2/28‑289 (MQ=255)
                                                                                                                                                    cggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAg                                                                                                                                                                                      <  1:450180‑M2/203‑1 (MQ=255)
                                                                                                                                                    cggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCg                                                                                                                                                           >  2:789370‑M2/28‑256 (MQ=255)
                                                                                                                                                    cggccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCg                                                                                                                                                           <  1:789370‑M2/229‑1 (MQ=255)
                                                                                                                                                      gccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCAATATCCCAGCCGCGATCGATTAAAATTTcc                                                                                                                        >  1:2295963‑M2/26‑289 (MQ=255)
                                                                                                                                                       ccatgttgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGGCCGAAACCGGAATACGGCATTTCGCTTCTCCCCGCGCTTCAAAACCCATTAACCCCGCGCATTCCCGAGCCGTTACCCGACGTCGACGATGTTGCTGATTCAGCGGACCG‑TCAAAGTCCTTCTCACGCGTTGCCATATCCCCACCGCGATCCATCAATATATcc                                                                                                                        >  2:468746‑M2/25‑288 (MQ=255)
                                                                                                                                                          tgttgcgcctcttgctgccacA‑ACGCCACCGCCTTTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCgcgc                                                                                                                    <  2:1911990‑M2/268‑1 (MQ=255)
                                                                                                                                                          ggttgtgcctgttgacgacgaA‑ACGCCACCGCCTGTTTGATTGTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCgcgc                                                                                                                    <  2:2372138‑M2/268‑1 (MQ=255)
                                                                                                                                                            tgtgtgcgcgtgcttacggA‑ACGCCAAGGCCTGTTTGATGTGTGGCTCAAGAAGTGTTGCCACCGCGGCAAAGAGCGGCACGCCCACCGAGTGACCGAACTGGCGATAGGCCTGAGTGTCCGAACCCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGcc                                                                                                                   <  2:2495568‑M2/269‑1 (MQ=255)
                                                                                                                                                             tgtgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCAt                                                                                                                 <  1:1788970‑M2/271‑1 (MQ=255)
                                                                                                                                                               tgcctcttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCAccc                                                                                                                                                            <  2:648803‑M2/228‑1 (MQ=255)
                                                                                                                                                                    cttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCCCCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCCCGCCATTTTTgtt                                                                                                          >  2:278324‑M2/12‑288 (MQ=255)
                                                                                                                                                                    ctTGCTGACGCA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCCCCACCACCGAGTTACCGAACCGGCGATAGGCCTCAGTTTCCGACACCGGAATACCGAATTTCGCCTCCCCCGGCGCTTCAAAACCCATTAAGCGCGCCCATTCCCGAGCAGCTACCCGCCCGGGCCGATGTTTTCTATTAAGCCGATCGACCAAAGT‑TTCCTCACACACGCCCATATCCGCGCCGCGCTCCATTAAAATCTCCTCTCCactttttta                                                                                                          >  2:2608962/1‑281 (MQ=255)
                                                                                                                                                                     ttgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCTACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATAT‑CCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                                                        <  2:927348‑M2/279‑1 (MQ=255)
                                                                                                                                                                      ttctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCCCGCGCATTCCCCAGGCGTTCACCGGCGTCGACGGTGTCTCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCAGATCCCAGCCGCTATCGATTAAAATTCCCGCCCCCTCATTGtcat                                                                                                        >  2:2207523‑M2/10‑289 (MQ=255)
                                                                                                                                                                      tgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGgcg                                                                                                                                                                                                                                                         >  2:391898‑M2/10‑145 (MQ=255)
                                                                                                                                                                      tgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCa                                                                                                                                                                           >  1:2013427‑M2/10‑222 (MQ=255)
                                                                                                                                                                      tgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCa                                                                                                                                                                           <  2:2013427‑M2/213‑1 (MQ=255)
                                                                                                                                                                      tgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                                                        <  1:1056417‑M2/280‑1 (MQ=255)
                                                                                                                                                                      tgctgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                                                        <  1:1772033‑M2/280‑1 (MQ=255)
                                                                                                                                                                         tgacgcA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaataa                                                                                                      <  1:1416117‑M2/282‑1 (MQ=255)
                                                                                                                                                                          gacgcA‑ACGCCACCGCCTTTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAAcg                                                                                                    <  1:74047‑M2/284‑1 (MQ=255)
                                                                                                                                                                               a‑aCGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCgcgc                                                                                                                    >  2:1797634/1‑268 (MQ=255)
                                                                                                                                                                               a‑aCGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCgcgc                                                                                                                    <  1:1797634/268‑1 (MQ=255)
                                                                                                                                                                                 aCGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGa                                                                                              <  1:2357491/289‑1 (MQ=255)
                                                                                                                                                                                  cGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGAc                                                                                             <  1:2656548/289‑1 (MQ=255)
                                                                                                                                                                                       ccGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAATCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCa                                                                                                                                                     <  1:2053157/228‑1 (MQ=255)
                                                                                                                                                                                            tGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCAGATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATACCGCGCAGACAGCGCAcgcg                                                                                   >  1:1009247/1‑289 (MQ=255)
                                                                                                                                                                                               ttGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCg                                                                                                                                                           <  2:2278336/214‑1 (MQ=255)
                                                                                                                                                                                                tGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGAc                                                                               <  2:1693054/289‑1 (MQ=255)
                                                                                                                                                                                                 gATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCCCAGACAGCGTACGCGTGAc                                                                               >  2:1975911/1‑288 (MQ=255)
                                                                                                                                                                                                       tGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGc                                                                        <  1:1975911/289‑1 (MQ=255)
                                                                                                                                                                                                                 aGTTTTGCCACCGAGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGt                                                                 <  2:1043102/286‑1 (MQ=255)
                                                                                                                                                                                                                             gcgGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCcgcg                                                                                                                                        <  2:1227558/203‑1 (MQ=255)
                                                                                                                                                                                                                                      aCCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCg                                                                                                                                                           >  1:704447/1‑175 (MQ=255)
                                                                                                                                                                                                                                                ccaccgagTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCGTTGCGGATTGt                                                                 >  2:452857/8‑255 (MQ=255)
                                                                                                                                                                                                                                                   ccgagTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACcgc                             >  1:1299384/5‑288 (MQ=255)
                                                                                                                                                                                                                                                          aCCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGa                     >  1:1336574/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          aCCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGa                     >  1:1210571/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           ccGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGAt                    >  2:191940/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              aaCTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCg                                          <  1:2150767/264‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaCTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCg                                          >  2:2150767/1‑264 (MQ=255)
                                                                                                                                                                                                                                                                cTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGATGtttt               >  1:1706906/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     gATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGATGTTTTTTCg           <  1:488340/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ttggCCTGAGTGTCCGAAACCGGAATACGAAATTTCGCTTCCCCCGGCGCTTAAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGATGTTTTTTCGCat        <  2:1332133/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                          gCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGATGTTTTTTCg           <  1:687071/283‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGCTGTTGCTGATTCAGCGGATCG‑TCCAAGTCCTTCTCACCCGTGGCCATATCCCATCCGCGACCGATTCAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCTGATTGTTCGGATAAACCATTCCATACCCGAAGCCGTTACCGCGCGCCTGATGTTTTTTCGCATAGCgca  >  2:1504698/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                       
CAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCTTAGTTATCGACTACGTCGGCCATGTTGTGCCTCTTGCTGACGCA‑ACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACGCTGTTACCGAACTGGCGATAGGCCTGAGTGTCGCTAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCG‑TCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACCGCGCGCCTGATGTTTTTTCGCATATCGAT  >  CP042583/1668287‑1668843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A