Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F89 I2 R1
|
21 |
29.0 |
1055738 |
89.7% |
946996 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,583,862 |
C→T |
R967H (CGT→CAT) |
hsdR ← |
endonuclease R Type I restriction enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,583,862 | 0 | C | T | 100.0%
| 64.6
/ NA
| 24 | R967H (CGT→CAT) | hsdR | endonuclease R Type I restriction enzyme |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/10); total (14/10) |
GAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGACGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCATAGCTCATCCAGACGACGCACCTGTTTAT > NC_000913/4583724‑4584000
|
gAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGc < 1:226860/143‑1 (MQ=255)
tCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCCTCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGATGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGAt > 2:392442/1‑143 (MQ=255)
gAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAATCCAGGCAGtt > 2:355887/1‑143 (MQ=255)
ccTGCGGCGTGTCGGAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTa < 1:415175/143‑1 (MQ=255)
tggggcgTGTGGTAATCCCCGTACAGCGATTTTACGCTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTaaa < 2:293262/140‑1 (MQ=255)
aaTCACCGTGCGGCGTGTTTGCGCGCGGCACTGCATCGTCGTTATCGCGGAAGATCGGCGCATCATTCAGGTTGTGGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTc < 2:461767/143‑1 (MQ=255)
tCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCa < 1:513076/143‑1 (MQ=255)
acTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGCTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCa > 1:415379/1‑143 (MQ=255)
tCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCACGAAGCTTGTTAAAGCATTCGGCGCTCCAGTGCGGCCCTTTTTCTCGCAgg > 2:483626/1‑143 (MQ=255)
ttCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGCgg > 1:348721/1‑143 (MQ=255)
tcgtcgATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGaa < 2:122262/143‑1 (MQ=255)
atatCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGtt > 2:276606/1‑143 (MQ=255)
atatCGAGGAAAATCGGCTCATCATT‑ACGTTTTTTTCGTCCCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGtt < 1:217007/143‑1 (MQ=255)
gAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCGGGCGCGAGGCCAAGCCGTCAAAGTTCa > 1:348145/1‑143 (MQ=255)
tccggTTGTTGACGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTgg < 1:3565/140‑1 (MQ=255)
aGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCa < 1:363991/143‑1 (MQ=255)
gATGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCCTAGCTCAt > 1:385717/1‑143 (MQ=255)
aTGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGCGGCGAACCCGTTAAAGTTCACGCCCGGCGCGTCCTGGGATCGCTccgc > 2:301091/1‑140 (MQ=255)
aTGT‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCAGAGCCCagc > 2:402882/1‑141 (MQ=255)
t‑CCGTTTTCAGCTTTTCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCATAGCTCATcccg > 1:374366/1‑141 (MQ=255)
cctttttCAGTTGTGCCAGTTGGGCCATAGACCCGTGTAGTTTGATATAGACTTCGGCGCTCCAGTGCGGCCCTTTTCCCCGCCGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCATAGCTCATCCAga < 2:58166/140‑1 (MQ=255)
tttCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCGTAGCTCATCCAGACGACGCACCTGt > 1:398737/1‑143 (MQ=255)
ttCCAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACACCCGCCGCGTCCTGGCATAGCTCATCCAGACGACGCACCTGta > 2:88646/1‑142 (MQ=255)
cAGATGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCAAGTGCGGCCCTTTTTCCCGCAGGCGCGCGGCGAAGCCGTTAAAGATCACGCCCGCCGCTGCCGGGCATAGCTCGTCCAGACGACGCCCCTGgttct > 2:454026/1‑141 (MQ=255)
|
GAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGT‑CCGTTTTCAGCTTTTCCAGACGGGCGATAAAGCCAGGCAGTTTGTTAAAGACTTCGGCGCTCCAGTGCGGCCCTTTTTCCCGCAGGCGCGAGGCGAAGCCGTTAAAGTTCACGCCCGCCGCGTCCTGGCATAGCTCATCCAGACGACGCACCTGTTTAT > NC_000913/4583724‑4584000
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
