Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,085,461	A→G	S151P (TCC→CCC)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,085,461	0	A	G	100.0%	249.4 / NA	69	S151P (TCC→CCC)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (37/32); total (37/32)

CAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAC  >  NC_000913/3085327‑3085594

cAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTAATGCCATCAACTCGGCTTTGGGACCg                                                                                                                                    <  2:373071/139‑1 (MQ=255)

          ttGTGCGCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCTTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑c                                                                                                                         >  1:129318/1‑139 (MQ=255)

          ttGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑c                                                                                                                         >  2:1051317/1‑139 (MQ=255)

            gtgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑ccc                                                                                                                       >  2:588024/1‑139 (MQ=255)

             tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                     >  2:1288555/1‑125 (MQ=255)

             tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                     <  1:1288555/125‑1 (MQ=255)

             tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCa                                                                                                                      >  1:466625/1‑139 (MQ=255)

             tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCa                                                                                                                      >  1:1369100/1‑139 (MQ=255)

                   aTCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGtt                                                                                                                >  1:1468456/1‑139 (MQ=255)

                       tctcGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGcc                                                                                                            >  2:1039810/1‑139 (MQ=255)

                              aaTTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCgaatga                                                                                                     <  1:1039810/139‑1 (MQ=255)

                               aTTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCgaatgaa                                                                                                    <  1:582376/139‑1 (MQ=255)

                                     gCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCgaat                                                                                                       >  2:352321/1‑130 (MQ=255)

                                     gCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCgaat                                                                                                       <  1:352321/130‑1 (MQ=255)

                                        aGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACt                                                                                           <  1:588024/139‑1 (MQ=255)

                                               tatatTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCAc                                                                                    >  2:339247/1‑139 (MQ=255)

                                                   ttCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGAGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                <  1:339247/139‑1 (MQ=255)

                                                    tCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcggc                                                                               >  2:1026871/1‑139 (MQ=255)

                                                      gcgGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGcggcgg                                                                             <  1:678624/139‑1 (MQ=255)

                                                        ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCCTCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           <  1:1051317/139‑1 (MQ=255)

                                                        ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           <  1:1026871/139‑1 (MQ=255)

                                                        ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           >  1:788248/1‑139 (MQ=255)

                                                                      ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                             <  2:58024/139‑1 (MQ=255)

                                                                      ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                             >  2:132905/1‑139 (MQ=255)

                                                                      ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                             >  2:461225/1‑139 (MQ=255)

                                                                      ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                             >  1:1431528/1‑139 (MQ=255)

                                                                      ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                             <  2:129318/139‑1 (MQ=255)

                                                                       tGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                            <  1:132905/139‑1 (MQ=255)

                                                                        gCAGACGATGACGCTACTGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCTGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATc                                                           <  2:336670/139‑1 (MQ=255)

                                                                        gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTACTGGTTAACTTCGAt                                                            >  2:948153/1‑139 (MQ=255)

                                                                        gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATc                                                           >  2:1057314/1‑139 (MQ=255)

                                                                           gACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATTAACTCGGCTTTGGGACCGGCTTCCAGACCCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTACTGGTTAACTTTGATCgg                                                         <  1:948153/139‑1 (MQ=255)

                                                                                tGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAGAc                                                   >  1:995500/1‑139 (MQ=255)

                                                                                tGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAAc                                                   <  2:1468456/139‑1 (MQ=255)

                                                                                        cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTgg                                                                       <  2:449419/111‑1 (MQ=255)

                                                                                        cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTgg                                                                       >  1:449419/1‑111 (MQ=255)

                                                                                        cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAAAAAGGAAGt                                           >  2:1326279/1‑139 (MQ=255)

                                                                                           gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           >  1:715822/1‑104 (MQ=255)

                                                                                           gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           <  2:715822/104‑1 (MQ=255)

                                                                                              aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGc                                     >  2:118020/1‑139 (MQ=255)

                                                                                                gCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGGTAAACAAGGAAGTAATCGCCg                                   <  1:491180/139‑1 (MQ=255)

                                                                                                gCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATa                                                      >  2:390383/1‑120 (MQ=255)

                                                                                                gCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATa                                                      <  1:390383/120‑1 (MQ=255)

                                                                                                   aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                                <  2:788248/139‑1 (MQ=255)

                                                                                                    gCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtat                               >  1:1119865/1‑139 (MQ=255)

                                                                                                      aTGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAg                             <  1:461225/139‑1 (MQ=255)

                                                                                                          gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCACTCACGcggcgg                                                                             <  1:852201/87‑1 (MQ=255)

                                                                                                          gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGcggcgg                                                                             >  2:852201/1‑87 (MQ=255)

                                                                                                          gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           >  2:1381038/1‑89 (MQ=255)

                                                                                                          gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           <  1:1381038/89‑1 (MQ=255)

                                                                                                          gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGt                         >  2:1060096/1‑139 (MQ=255)

                                                                                                              gTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGa                     >  1:334431/1‑139 (MQ=255)

                                                                                                               tACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGAt                    >  1:965984/1‑139 (MQ=255)

                                                                                                                   gCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATa                                                      >  2:308152/1‑101 (MQ=255)

                                                                                                                   gCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATa                                                      <  1:308152/101‑1 (MQ=255)

                                                                                                                   gCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTccc                >  2:1320472/1‑139 (MQ=255)

                                                                                                                   gCCATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTccc                >  1:1130622/1‑139 (MQ=255)

                                                                                                                    ccATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                >  1:474891/1‑74 (MQ=255)

                                                                                                                    ccATCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                <  2:474891/74‑1 (MQ=255)

                                                                                                                      aTCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCAcg                                                                                   >  2:221201/1‑69 (MQ=255)

                                                                                                                      aTCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCAcg                                                                                   <  1:221201/69‑1 (MQ=255)

                                                                                                                      aTCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGt                                                                      >  1:689966/1‑82 (MQ=255)

                                                                                                                      aTCAACTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGt                                                                      <  2:689966/82‑1 (MQ=255)

                                                                                                                           cTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGc                                                                                                             <  2:1065431/38‑1 (MQ=255)

                                                                                                                           cTCGGCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGc                                                                                                             >  1:1065431/1‑38 (MQ=255)

                                                                                                                              ggCTTTGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTg     <  1:192917/139‑1 (MQ=255)

                                                                                                                                 tttGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           >  1:994729/1‑66 (MQ=255)

                                                                                                                                 tttGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                           <  2:994729/66‑1 (MQ=255)

                                                                                                                                 tttGGGACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAc  <  1:1326279/139‑1 (MQ=255)

CAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGA‑CCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAC  >  NC_000913/3085327‑3085594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A