Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,547,986	C→T	695 (TAG→TAA)	malQ ←	4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,547,986	0	C	T	100.0%	151.1 / NA	47	695 (TAG→TAA)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/21); total (26/21)

TCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGT  >  NC_000913/3547851‑3548117

tCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTAc                                                                                                                                  >  2:89582/1‑139 (MQ=255)

tCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTAc                                                                                                                                  >  1:22718/1‑139 (MQ=255)

                    tttGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGctct                                                                                                              >  1:1209336/1‑139 (MQ=255)

                    tttGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGctct                                                                                                              >  2:97744/1‑139 (MQ=255)

                    tttGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGctct                                                                                                              >  2:1302488/1‑139 (MQ=255)

                         tAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                          <  1:1191623/122‑1 (MQ=255)

                         tAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                          >  2:1191623/1‑122 (MQ=255)

                               tgactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTc                                                                                                      <  1:140728/136‑1 (MQ=255)

                               tgactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTc                                                                                                      >  2:140728/1‑136 (MQ=255)

                                 actgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTcc                                                                                                 >  2:280004/1‑139 (MQ=255)

                                  ctgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTcc                                                                                                 >  2:555509/1‑139 (MQ=255)

                                     ccGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAAt                                                                                             >  2:1476435/1‑139 (MQ=255)

                                      cGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATc                                                                                            <  1:1302488/139‑1 (MQ=255)

                                           gcggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                       <  1:97744/139‑1 (MQ=255)

                                                gTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAAc                                                                                  <  1:89582/139‑1 (MQ=255)

                                                    ccGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGt                                                                              >  1:789552/1‑139 (MQ=255)

                                                           aTCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGcc                                                                       <  2:22718/139‑1 (MQ=255)

                                                                          cGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                     <  1:610869/78‑1 (MQ=255)

                                                                          cGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                     >  2:610869/1‑78 (MQ=255)

                                                                           gggATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                       >  1:202766/1‑91 (MQ=255)

                                                                           gggATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                       <  2:202766/91‑1 (MQ=255)

                                                                           gggATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                       >  1:941576/1‑107 (MQ=255)

                                                                           ggcaTGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                       <  2:941576/104‑1 (MQ=255)

                                                                                      gtagGCCGGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCg                                             <  1:555509/139‑1 (MQ=255)

                                                                                       tagGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                       >  1:655047/1‑95 (MQ=255)

                                                                                       tagGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                       <  2:655047/95‑1 (MQ=255)

                                                                                           cc‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                       >  2:515717/1‑139 (MQ=255)

                                                                                           cc‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                       >  2:42783/1‑139 (MQ=255)

                                                                                           cc‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                       >  2:1302130/1‑139 (MQ=255)

                                                                                                       gcgTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                          >  2:1448946/1‑45 (MQ=255)

                                                                                                       gcgTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                          <  1:1448946/45‑1 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCg                                                                                                        <  2:587462/60‑1 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCg                                                                                                        >  1:587462/1‑60 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAAt                                                                                             <  2:903417/71‑1 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAAt                                                                                             >  1:903417/1‑71 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAg                                   >  1:1302335/1‑129 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAg                                   <  2:1302335/129‑1 (MQ=255)

                                                                                                          tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                         >  1:1474676/1‑139 (MQ=255)

                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                        <  1:42783/139‑1 (MQ=255)

                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                        <  2:789552/139‑1 (MQ=255)

                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                        <  2:1474676/139‑1 (MQ=255)

                                                                                                              gcatcacatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGt                     <  2:382527/139‑1 (MQ=255)

                                                                                                                atcgcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGttt                   <  1:280004/139‑1 (MQ=255)

                                                                                                                atcgcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGttt                   >  2:1152476/1‑139 (MQ=255)

                                                                                                                    catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTtat               >  2:326211/1‑139 (MQ=255)

                                                                                                                         ggCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTgg          >  1:840004/1‑139 (MQ=255)

                                                                                                                                 ccGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGt  <  2:1209336/139‑1 (MQ=255)

TCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCC‑GGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGT  >  NC_000913/3547851‑3548117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A