Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,815,811 |
C→T |
intergenic (‑43/+23) |
pyrE ← / ← rph |
orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,815,811 | 0 | C | T | 100.0%
| 139.3
/ NA
| 44 | intergenic (‑43/+23) | pyrE/rph | orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/23); total (21/23) |
CCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGT > NC_000913/3815685‑3815943
|
cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCg < 2:126949/139‑1 (MQ=255)
cGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGcc < 1:20037/139‑1 (MQ=255)
aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGtt > 1:459071/1‑139 (MQ=255)
aaaCTCGCCAAACTTTAACACCCGCTTGCTAAGCGCAAATTCAATAAACTGGCGCCGATATGGTTTAATGCATTGGCTCCCCATCTTACTTTTCTACAGAC‑AAAAACAGGTAACTCATCATTCGCCTTAACAATCAGtt > 1:1216401/1‑139 (MQ=255)
ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCg < 1:951285/117‑1 (MQ=255)
ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCg > 2:951285/1‑117 (MQ=255)
cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg < 1:450815/139‑1 (MQ=255)
ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg < 2:714425/139‑1 (MQ=255)
tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCg < 1:161436/102‑1 (MQ=255)
tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCg > 2:161436/1‑102 (MQ=255)
ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACa < 2:480623/139‑1 (MQ=255)
aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTATTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccc < 2:459071/139‑1 (MQ=255)
aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt < 2:1159869/139‑1 (MQ=255)
gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaacaa > 1:34960/1‑139 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgc > 1:454652/1‑79 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgc < 2:454652/79‑1 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg < 1:66374/92‑1 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg > 2:66374/1‑92 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg > 2:1141732/1‑135 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg < 1:1141732/135‑1 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGa > 1:804051/1‑139 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc > 1:53715/1‑93 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc < 2:53715/93‑1 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg > 1:334682/1‑139 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg < 2:1274671/139‑1 (MQ=255)
cTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg < 1:306151/139‑1 (MQ=255)
tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg > 1:275997/1‑134 (MQ=255)
tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg < 2:275997/134‑1 (MQ=255)
tcctcATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGt > 2:203091/1‑45 (MQ=255)
tcctcATCTTACTTTTCTACAGAC‑AAAAAAAGGTGACTCATCAGt < 1:203091/45‑1 (MQ=255)
tCTACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc > 1:1039414/1‑139 (MQ=255)
tACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcga < 2:1092350/83‑1 (MQ=255)
tACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcga > 1:1092350/1‑83 (MQ=255)
tACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg < 1:843661/113‑1 (MQ=255)
tACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg > 2:843661/1‑113 (MQ=255)
tACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg < 2:1440739/134‑1 (MQ=255)
tACAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg > 1:1440739/1‑134 (MQ=255)
cAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt > 1:709210/1‑64 (MQ=255)
cAGAC‑AAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt < 2:709210/64‑1 (MQ=255)
aaaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt < 1:721896/114‑1 (MQ=255)
aaaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt > 2:721896/1‑114 (MQ=255)
aaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt > 2:850509/1‑139 (MQ=255)
aaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt < 2:979285/78‑1 (MQ=255)
aaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt > 1:979285/1‑78 (MQ=255)
|
CCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGT > NC_000913/3815685‑3815943
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
