Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,056,273	G→A	P11P (CCG→CCA)	fau →	5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,056,273	0	G	A	100.0%	217.5 / NA	67	P11P (CCG→CCA)	fau	5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/33); total (34/33)

CTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAAC  >  NC_000913/3056143‑3056403

cTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAacaaca                                                                                                                            <  2:1175412/139‑1 (MQ=255)

 tGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                           >  1:1613144/1‑139 (MQ=255)

 tGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                           >  2:34696/1‑139 (MQ=255)

  gcggcgGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcagc                                                                                                                          >  1:658622/1‑139 (MQ=255)

   cggcggCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcagca                                                                                                                         >  1:771258/1‑139 (MQ=255)

                  gaTTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaaca                                                                                                          <  2:771258/139‑1 (MQ=255)

                  gaTTCCCTTCTTATCTGGCACCAGCCAGGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaaca                                                                                                          <  1:479314/139‑1 (MQ=255)

                      cccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGCTTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGcc                                                                                                      >  1:977088/1‑139 (MQ=255)

                                 tGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                           >  2:726022/1‑139 (MQ=255)

                                  ggCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                          <  1:726022/139‑1 (MQ=255)

                                  ggCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                          <  2:79410/139‑1 (MQ=255)

                                   gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatga                                                                                         >  1:696604/1‑139 (MQ=255)

                                    cACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                        >  2:779141/1‑139 (MQ=255)

                                     aCCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGatgatg                                                                                       >  2:287990/1‑139 (MQ=255)

                                        aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                           <  2:583987/100‑1 (MQ=255)

                                        aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                           >  1:583987/1‑100 (MQ=255)

                                         gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACtt                                                                                   >  1:678090/1‑139 (MQ=255)

                                                  cAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCgg                                                                          <  1:662224/139‑1 (MQ=255)

                                                   aaCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggt                                                                         <  1:779141/139‑1 (MQ=255)

                                                   aaCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggt                                                                         <  1:287990/139‑1 (MQ=255)

                                                       aCCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     <  1:509942/139‑1 (MQ=255)

                                                       aCCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     <  2:1613144/139‑1 (MQ=255)

                                                               tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCAGGTGGTGATGGcaca                                                             >  1:606121/1‑139 (MQ=255)

                                                                cccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACAt                                                            >  1:46344/1‑139 (MQ=255)

                                                                cccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACAt                                                            >  2:129150/1‑139 (MQ=255)

                                                                 ccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                           >  1:185485/1‑139 (MQ=255)

                                                                      gACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAATGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATccc                                                                              <  2:66600/115‑1 (MQ=255)

                                                                      gACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAATGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATccc                                                                              >  1:66600/1‑115 (MQ=255)

                                                                       aCATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                                     <  1:129150/139‑1 (MQ=255)

                                                                         aTTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCgg                                                                                             >  2:724756/1‑97 (MQ=255)

                                                                         aTTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCgg                                                                                             <  1:724756/97‑1 (MQ=255)

                                                                            aTCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                                <  1:1110042/139‑1 (MQ=255)

                                                                             tCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                        <  2:1430502/98‑1 (MQ=255)

                                                                             tCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                        >  1:1430502/1‑98 (MQ=255)

                                                                               ccGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTc                                             <  1:756411/139‑1 (MQ=255)

                                                                                 gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                       >  1:1193652/1‑79 (MQ=255)

                                                                                 gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                       <  2:1193652/79‑1 (MQ=255)

                                                                                 gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGcaca                                                             <  1:854949/121‑1 (MQ=255)

                                                                                 gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGcaca                                                             >  2:854949/1‑121 (MQ=255)

                                                                                  actaGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                                      >  1:935584/4‑111 (MQ=255)

                                                                                  aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGatgatg                                                                                       >  1:1138018/1‑94 (MQ=255)

                                                                                  aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGatgatg                                                                                       <  2:1138018/94‑1 (MQ=255)

                                                                                  aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                                      <  2:935584/111‑1 (MQ=255)

                                                                                  aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCctc                                          >  1:1540883/1‑139 (MQ=255)

                                                                                   cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                        <  1:1144334/92‑1 (MQ=255)

                                                                                   cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                        >  2:1144334/1‑92 (MQ=255)

                                                                                      gAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     >  2:551027/1‑108 (MQ=255)

                                                                                      gAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     <  1:551027/108‑1 (MQ=255)

                                                                                         aTCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                                >  2:1350957/1‑126 (MQ=255)

                                                                                         aTCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                                <  1:1350957/126‑1 (MQ=255)

                                                                                              cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGc                              <  2:977088/139‑1 (MQ=255)

                                                                                                      ttCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGa                      <  1:900131/139‑1 (MQ=255)

                                                                                                           cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                           >  2:1578350/1‑49 (MQ=255)

                                                                                                           cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                           <  1:1578350/49‑1 (MQ=255)

                                                                                                           cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGAc                                                                                     <  1:219218/71‑1 (MQ=255)

                                                                                                           cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGAc                                                                                     >  2:219218/1‑71 (MQ=255)

                                                                                                              cgtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGc              <  2:46344/139‑1 (MQ=255)

                                                                                                                tcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCa            >  2:759880/1‑139 (MQ=255)

                                                                                                                    cgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCa        <  1:34696/139‑1 (MQ=255)

                                                                                                                    cgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCa        >  1:509857/1‑139 (MQ=255)

                                                                                                                         gTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTa                                                                                  <  1:958468/60‑1 (MQ=255)

                                                                                                                         gTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTa                                                                                  >  2:958468/1‑60 (MQ=255)

                                                                                                                         gTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGAt                                                                   <  1:250669/75‑1 (MQ=255)

                                                                                                                         gTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGAt                                                                   >  2:250669/1‑75 (MQ=255)

                                                                                                                          ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGaac  >  1:709054/1‑139 (MQ=255)

                                                                                                                            aaCGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCt                                                   <  2:1468057/88‑1 (MQ=255)

                                                                                                                            aaCGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCt                                                   >  1:1468057/1‑88 (MQ=255)

CTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAAC  >  NC_000913/3056143‑3056403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A