Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,813	A→G	intergenic (‑45/+21)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,813	0	A	G	100.0%	206.5 / NA	59	intergenic (‑45/+21)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/31); total (28/31)

TTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCC  >  NC_000913/3815680‑3815947

ttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCtcat                                                                                                                                   <  2:39053/139‑1 (MQ=255)

              cAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTaaaa                                                                                                                     <  1:847774/139‑1 (MQ=255)

                      aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGttt                                                                                                             >  2:696647/1‑139 (MQ=255)

                        tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                           <  1:416092/139‑1 (MQ=255)

                          gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCa                                                                                                         <  2:1307353/139‑1 (MQ=255)

                           ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAg                                                                                                        >  1:703247/1‑139 (MQ=255)

                             aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                      >  2:413513/1‑139 (MQ=255)

                               aCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                    <  1:1579351/139‑1 (MQ=255)

                                    aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCtt                                                                                               <  1:1202585/139‑1 (MQ=255)

                                         cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                                                                          >  1:157157/1‑139 (MQ=255)

                                             ttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCgt                                                                                      <  1:696647/139‑2 (MQ=255)

                                                      gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGga                                                                             >  1:1232506/1‑139 (MQ=255)

                                                        aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgatt                                                                           <  2:703247/139‑1 (MQ=255)

                                                         aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCt                                                                                                                                      <  1:237438/79‑1 (MQ=255)

                                                               aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTcc                                                                    >  1:421284/1‑139 (MQ=255)

                                                                     tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                         >  1:1017521/1‑112 (MQ=255)

                                                                     tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                         <  2:1017521/112‑1 (MQ=255)

                                                                       gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                            <  2:157157/139‑1 (MQ=255)

                                                                        cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                 <  2:510874/133‑1 (MQ=255)

                                                                        cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                 >  1:510874/1‑133 (MQ=255)

                                                                              tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGcc                                                     <  1:1396227/139‑1 (MQ=255)

                                                                                   tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTCCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaacaa                                                >  1:836264/1‑139 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGt                                                                                                                               >  2:834025/1‑56 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGt                                                                                                                               <  1:834025/56‑1 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                         <  1:1447887/94‑1 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                         >  2:1447887/1‑94 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                 >  2:1359176/1‑118 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                 <  1:1359176/118‑1 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                            <  1:266448/123‑1 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                            <  2:1332282/123‑1 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                            >  1:1332282/1‑123 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                            >  2:266448/1‑123 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaaca                                                 <  1:6425/134‑1 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaaca                                                 >  2:6425/1‑134 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                            >  1:72677/1‑139 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                            >  2:473523/1‑139 (MQ=255)

                                                                                       aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                            >  2:879385/1‑139 (MQ=255)

                                                                                         gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                                          <  2:1232506/139‑1 (MQ=255)

                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCt                                                                                                                                      >  1:430507/1‑44 (MQ=255)

                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                 <  1:1044477/113‑1 (MQ=255)

                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                 >  2:1044477/1‑113 (MQ=255)

                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                       <  1:879385/139‑1 (MQ=255)

                                                                                            ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                       <  1:626809/139‑1 (MQ=255)

                                                                                              cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGTGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                     <  1:618185/139‑1 (MQ=255)

                                                                                              cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                      <  2:1580487/106‑1 (MQ=255)

                                                                                              cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                      >  1:1580487/1‑106 (MQ=255)

                                                                                                   ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                         <  2:177870/82‑1 (MQ=255)

                                                                                                   ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                         >  1:177870/1‑82 (MQ=255)

                                                                                                               ttCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCtt                                                                                                                         <  2:1094635/38‑1 (MQ=255)

                                                                                                               ttCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCtt                                                                                                                         >  1:1094635/1‑38 (MQ=255)

                                                                                                               ttCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATg                                                                               <  2:1268602/80‑1 (MQ=255)

                                                                                                               ttCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATg                                                                               >  1:1268602/1‑80 (MQ=255)

                                                                                                               ttCTACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                    >  1:891681/1‑139 (MQ=255)

                                                                                                                  tACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                                                                              <  2:218441/62‑1 (MQ=255)

                                                                                                                  tACAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                                                                              >  1:218441/1‑62 (MQ=255)

                                                                                                                   aCAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                <  2:891681/139‑1 (MQ=255)

                                                                                                                    cAGACAAAAAAAAGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc               <  2:836264/139‑1 (MQ=255)

                                                                                                                         aaaaaaaaGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg          >  1:847442/1‑139 (MQ=255)

                                                                                                                          aaaaaaaGGCGGCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc         >  1:1228097/1‑139 (MQ=255)

                                                                                                                                 ggcggcTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgat                                                                       >  2:531180/1‑70 (MQ=255)

                                                                                                                                 ggcggcTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgat                                                                       <  1:531180/70‑1 (MQ=255)

                                                                                                                                 ggcggcTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGcc                                                           <  1:1597408/82‑1 (MQ=255)

                                                                                                                                 ggcggcTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGcc                                                           >  2:1597408/1‑82 (MQ=255)

                                                                                                                                 ggcggcTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc  >  1:349533/1‑139 (MQ=255)

TTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCC  >  NC_000913/3815680‑3815947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A