Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,061,157	G→A	237 (TAG→TAA)	yihL →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,061,157	0	G	A	100.0%	164.1 / NA	51	237 (TAG→TAA)	yihL	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/25); total (26/25)

TATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAT  >  NC_000913/4061026‑4061281

tATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGtttt                                                                                                                       >  1:435687/1‑139 (MQ=255)

tATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGtttt                                                                                                                       >  1:859423/1‑139 (MQ=255)

                     aTGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCa                                                                                                  >  2:1240033/1‑139 (MQ=255)

                          aGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                             <  2:435687/139‑1 (MQ=255)

                             gATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGAt                                                                                          >  1:1093290/1‑139 (MQ=255)

                                 ttAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTa                                                                                      >  2:1111369/1‑139 (MQ=255)

                                          aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTa                                                                                                                      >  2:1503689/1‑98 (MQ=255)

                                          aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTa                                                                                                                      <  1:1503689/98‑1 (MQ=255)

                                                  cgcCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              >  2:1443115/1‑114 (MQ=255)

                                                  cgcCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  1:1443115/114‑1 (MQ=255)

                                                            cAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttat                                                           >  1:892931/1‑139 (MQ=255)

                                                                gCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc                                                       <  2:859423/139‑1 (MQ=255)

                                                                 cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                              >  1:1098768/1‑83 (MQ=255)

                                                                 cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                              <  2:1098768/83‑1 (MQ=255)

                                                                         tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATg                                              >  1:1371474/1‑139 (MQ=255)

                                                                         tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATg                                              >  1:322909/1‑139 (MQ=255)

                                                                          ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTc                                                                             <  1:1261808/107‑1 (MQ=255)

                                                                          ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTc                                                                             >  2:1261808/1‑107 (MQ=255)

                                                                          ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                             >  1:1179444/1‑139 (MQ=255)

                                                                            aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                           <  2:322909/139‑1 (MQ=255)

                                                                            aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                           <  1:1255051/139‑1 (MQ=255)

                                                                             aaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCtt                                          <  2:522674/139‑1 (MQ=255)

                                                                             aaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCtt                                          <  1:912331/139‑1 (MQ=255)

                                                                              aTAGATATTGAATATTGGTGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCttt                                         >  2:1299606/1‑139 (MQ=255)

                                                                              aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCttt                                         <  2:892931/139‑1 (MQ=255)

                                                                                   tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                   >  1:540506/1‑60 (MQ=255)

                                                                                   tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                   <  2:540506/60‑1 (MQ=255)

                                                                                   tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCAttt                                    >  2:1081988/1‑139 (MQ=255)

                                                                                       gAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                        <  1:1051653/115‑1 (MQ=255)

                                                                                       gAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                        >  2:1051653/1‑115 (MQ=255)

                                                                                        aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                           >  2:985655/1‑127 (MQ=255)

                                                                                        aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAata                               <  1:122222/139‑1 (MQ=255)

                                                                                        aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACACTTCCTCTTTATTTACATGCTTATGCCt                                           <  1:985655/127‑1 (MQ=255)

                                                                                               gtgATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                        <  1:1299606/137‑1 (MQ=255)

                                                                                               gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   <  1:435530/80‑1 (MQ=255)

                                                                                               gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   >  2:435530/1‑80 (MQ=255)

                                                                                               gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                        >  2:854262/1‑139 (MQ=255)

                                                                                                  aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              >  2:294365/1‑66 (MQ=255)

                                                                                                  aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  1:294365/66‑1 (MQ=255)

                                                                                                  aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg                             <  2:283994/131‑1 (MQ=255)

                                                                                                  aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg                             >  1:283994/1‑131 (MQ=255)

                                                                                                  aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGa                     >  2:90183/1‑139 (MQ=255)

                                                                                                      gAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAaga                 <  1:1240033/139‑1 (MQ=255)

                                                                                                               gACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCct        <  1:1111369/139‑1 (MQ=255)

                                                                                                                     atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCg                           <  1:38448/114‑1 (MQ=255)

                                                                                                                     atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCg                           >  2:38448/1‑114 (MQ=255)

                                                                                                                     atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt  <  2:1093290/139‑1 (MQ=255)

                                                                                                                       catTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttat                                                           <  2:1081285/80‑1 (MQ=255)

                                                                                                                       catTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttat                                                           >  1:1081285/1‑80 (MQ=255)

                                                                                                                               tGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                        >  1:1093381/1‑75 (MQ=255)

                                                                                                                               tGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                        <  2:1093381/75‑1 (MQ=255)

TATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAT  >  NC_000913/4061026‑4061281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A