Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,850,197	T→C	Y80C (TAC→TGC)	hycA ←	regulator of the transcriptional regulator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,850,197	0	T	C	98.8%	286.8 / ‑4.8	81	Y80C (TAC→TGC)	hycA	regulator of the transcriptional regulator FhlA
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (42/38); total (43/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

CAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGTAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTG  >  NC_000913/2850063‑2850331

cAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATa                                                                                                                                    >  2:1238830/1‑139 (MQ=255)

  gCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACt                                                                                                                                  >  2:1338422/1‑139 (MQ=255)

             gagaTAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTgg                                                                                                                       <  1:1199704/139‑1 (MQ=255)

                        ccAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGcc                                                                                                            <  1:1338422/139‑1 (MQ=255)

                         cAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGcc                                                                                                            <  1:852056/138‑1 (MQ=255)

                         cAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGcc                                                                                                            >  2:852056/1‑138 (MQ=255)

                           gAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATc                                                                                                         >  1:522225/1‑139 (MQ=255)

                            aaCCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACt                                                                                                                                  <  2:1519258/113‑1 (MQ=255)

                            aaCCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACt                                                                                                                                  >  1:1519258/1‑113 (MQ=255)

                            aaCCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATcc                                                                                                        >  1:219741/1‑139 (MQ=255)

                             aCCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCg                                                                                                       <  1:1238830/139‑1 (MQ=255)

                              ccTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGc                                                                                                      >  2:166351/1‑139 (MQ=255)

                               cTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGcc                                                                                                     <  1:1376747/139‑1 (MQ=255)

                               cTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGcc                                                                                                     <  2:581090/139‑1 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTa                                                                                                                 <  2:335264/125‑1 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTa                                                                                                                 >  1:335264/1‑125 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCa                                                                                                           <  2:833618/131‑1 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCa                                                                                                           <  2:786076/131‑1 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCa                                                                                                           >  1:833618/1‑131 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCa                                                                                                           >  1:786076/1‑131 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAg                                                                                                   >  2:1308978/1‑139 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAg                                                                                                   >  2:815443/1‑139 (MQ=255)

                                 gTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAg                                                                                                   <  2:93962/139‑1 (MQ=255)

                                      cgATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCaa                                                                                                                                      >  1:1597706/1‑99 (MQ=255)

                                      cgATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCaa                                                                                                                                      <  2:1597706/99‑1 (MQ=255)

                                      cgATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGa                                                                                              >  1:1121351/1‑139 (MQ=255)

                                                   tGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGaaa                                                                                 <  1:166351/139‑1 (MQ=255)

                                                    gACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGa                                                                                                                               <  2:145671/92‑1 (MQ=255)

                                                    gACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGa                                                                                                                               >  1:145671/1‑92 (MQ=255)

                                                       cTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGtgtg                                                                                                                        >  2:1521678/1‑96 (MQ=255)

                                                       cTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGtgtg                                                                                                                        <  1:1521678/96‑1 (MQ=255)

                                                          gCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCa                                                                          >  1:212558/1‑139 (MQ=255)

                                                               caatcaTGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGTAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAg                                                                     >  2:190592/1‑139 (MQ=255)

                                                               caatcaTGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCaaa                                                                        <  1:1035314/136‑1 (MQ=255)

                                                               caatcaTGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCaaa                                                                        >  2:1035314/1‑136 (MQ=255)

                                                               caatcaTGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAg                                                                     >  1:679802/1‑139 (MQ=255)

                                                                 atcaTGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGa                                                                   <  2:1318794/139‑1 (MQ=255)

                                                                     tGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGc                                                                                                      <  2:666338/100‑1 (MQ=255)

                                                                     tGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGc                                                                                                      >  1:666338/1‑100 (MQ=255)

                                                                     tGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGc                                                                                                      <  1:1641561/100‑1 (MQ=255)

                                                                     tGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGc                                                                                                      >  2:1641561/1‑100 (MQ=255)

                                                                     tGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGaa                                                               >  1:714654/1‑139 (MQ=255)

                                                                     tGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGaa                                                               >  1:1613417/1‑139 (MQ=255)

                                                                      gCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATc                                                                                                         <  2:398174/96‑1 (MQ=255)

                                                                      gCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATc                                                                                                         >  1:398174/1‑96 (MQ=255)

                                                                          tCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACaga                                                          >  1:1224494/1‑139 (MQ=255)

                                                                            aaTGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCa                                                                                                           <  2:1226068/88‑1 (MQ=255)

                                                                            aaTGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCa                                                                                                           >  1:1226068/1‑88 (MQ=255)

                                                                                    gTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTATTTGTc                                                >  1:1460887/1‑139 (MQ=255)

                                                                                        cgcCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGgc                                            >  2:552690/1‑139 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGtgtg                                                                                                                        <  2:964354/61‑1 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGtgtg                                                                                                                        >  1:964354/1‑61 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTgg                                                                                                                       <  2:594492/62‑1 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTgg                                                                                                                       >  1:594492/1‑62 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTaa                                                                                                                >  2:558757/1‑69 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTaa                                                                                                                <  1:558757/69‑1 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGgcgc                                          >  1:1383456/1‑139 (MQ=255)

                                                                                          ccTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGgcgc                                          >  2:1651152/1‑139 (MQ=255)

                                                                                             gcgcAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATg                                                                              >  2:218223/1‑100 (MQ=255)

                                                                                             gcgcAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATg                                                                              <  1:218223/100‑1 (MQ=255)

                                                                                             gcgcAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCa                                       >  2:1237965/1‑139 (MQ=255)

                                                                                             gcacAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACg                                                                 <  1:150296/110‑1 (MQ=255)

                                                                                             gcacAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACg                                                                 >  2:150296/4‑113 (MQ=255)

                                                                                               gcAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGc                                     >  2:1253061/1‑139 (MQ=255)

                                                                                                 aaTCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTc                                   <  2:679802/139‑1 (MQ=255)

                                                                                                       cTGTTTGTCTTCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCg                             >  2:427047/1‑139 (MQ=255)

                                                                                                                 tCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCaaa                                                                        <  2:944090/86‑1 (MQ=255)

                                                                                                                 tCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCaaa                                                                        >  1:944090/1‑86 (MQ=255)

                                                                                                                 tCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGgc                   <  2:522225/139‑1 (MQ=255)

                                                                                                                 tCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGgc                   <  1:427047/139‑1 (MQ=255)

                                                                                                                 tCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGgc                   <  2:134169/139‑1 (MQ=255)

                                                                                                                 tCGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGgc                   <  2:1383456/139‑1 (MQ=255)

                                                                                                                  cGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGaaa                                                                                 >  2:220402/1‑76 (MQ=255)

                                                                                                                  cGCCATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGaaa                                                                                 <  1:220402/76‑1 (MQ=255)

                                                                                                                    ccATCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGgcgcg                <  2:1460887/139‑1 (MQ=255)

                                                                                                                       tCTTTTGTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCtt             >  2:1385095/1‑139 (MQ=255)

                                                                                                                             gTTTCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATa       <  2:714654/139‑1 (MQ=255)

                                                                                                                              tttCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGaaa                                                                                 <  1:768546/64‑1 (MQ=255)

                                                                                                                              tttCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGaaa                                                                                 >  2:768546/1‑64 (MQ=255)

                                                                                                                                tCGACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACg    <  2:219741/139‑1 (MQ=255)

                                                                                                                                  gACGCAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTg  <  2:1224494/139‑1 (MQ=255)

CAGCGATTTTTTCGAGATAGTGTTCCAGAACCTGTTCGCGATCGCGGATGTTGACCTTGCCATCAATCATGCCGTCAATGCTCAGTTGCGCCTGCGCAATCCGCTGTTTGTCTTCGCCATCTTTTGTTTCGACGTAATACTCGATGGTGTGGCTGTTAAAGCCATCCGCCAGGGTGACGTAAATGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTG  >  NC_000913/2850063‑2850331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A