Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,761,850 C→T A36T (GCG→ACG)  yibF ← glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,761,8500CT100.0% 195.3 / NA 61A36T (GCG→ACG) yibFglutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/29);  total (32/29)

TCGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGCGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAATATC  >  NC_000913/3761721‑3761984
                                                                                                                                 |                                                                                                                                      
tCGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                               <  2:1259182/139‑1 (MQ=255)
                  ttAATTCAATATACTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTc                                                                                                             >  1:1527620/1‑139 (MQ=255)
                    aaTTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                           <  2:684782/139‑1 (MQ=255)
                             tatTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  <  2:1527620/139‑1 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                               <  2:1321197/107‑1 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                               >  1:1321197/1‑107 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGATAGTTATGCCCt                                                                                               <  1:326611/139‑1 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  >  2:34583/1‑136 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  <  1:34583/136‑1 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                               >  2:195813/1‑139 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                               <  2:746349/139‑1 (MQ=255)
                                tCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                               <  1:195813/139‑1 (MQ=255)
                                    cGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTc                                                                                           >  1:757974/1‑139 (MQ=255)
                                     gatgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                              >  2:359699/1‑103 (MQ=255)
                                     gatgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                              <  1:359699/103‑1 (MQ=255)
                                     gatgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCt                                                                                          >  1:156492/1‑139 (MQ=255)
                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                           <  2:363121/118‑1 (MQ=255)
                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                           >  1:363121/1‑118 (MQ=255)
                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  <  2:76479/127‑1 (MQ=255)
                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  >  1:76479/1‑127 (MQ=255)
                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaaca                                                                                      >  1:1292882/1‑139 (MQ=255)
                                            ggCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAacaaca                                                                                   <  2:757974/139‑1 (MQ=255)
                                            ggCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAacaaca                                                                                   >  1:1276716/1‑139 (MQ=255)
                                              cGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAg                                                                                                                            <  1:487261/96‑1 (MQ=255)
                                              cGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAg                                                                                                                            >  2:487261/1‑96 (MQ=255)
                                              cGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaa                                                                                     <  1:1276245/135‑1 (MQ=255)
                                              cGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaa                                                                                     >  2:1276245/1‑135 (MQ=255)
                                                  tCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAg                                                                             >  1:1359477/1‑139 (MQ=255)
                                                       ccAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGt                                                                        <  2:1359477/139‑1 (MQ=255)
                                                           cATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCg                                                                                                            <  1:1390995/99‑1 (MQ=255)
                                                           cATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCg                                                                                                            >  2:1390995/1‑99 (MQ=255)
                                                           cATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGc                                                                    >  1:1031002/1‑139 (MQ=255)
                                                                      ttCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACggg                                                         <  2:1276716/139‑1 (MQ=255)
                                                                      ttCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACggg                                                         <  2:1292882/139‑1 (MQ=255)
                                                                          gTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCt                                                                                          <  2:570115/102‑1 (MQ=255)
                                                                          gTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCt                                                                                          >  1:570115/1‑102 (MQ=255)
                                                                              ccAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACaa                                                              >  1:179784/1‑126 (MQ=255)
                                                                              ccAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACaa                                                              <  2:179784/126‑1 (MQ=255)
                                                                                aGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                               >  2:1443424/1‑91 (MQ=255)
                                                                                aGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                               <  1:1443424/91‑1 (MQ=255)
                                                                                aGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGc                                               >  1:1554351/1‑139 (MQ=255)
                                                                                aGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGc                                               >  1:1339199/1‑139 (MQ=255)
                                                                                         aCTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACcgacga                                      <  2:1554351/139‑1 (MQ=255)
                                                                                           ttttCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGt                                    >  1:1078859/1‑139 (MQ=255)
                                                                                                  aaCGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGc                             <  2:1339199/139‑1 (MQ=255)
                                                                                                     gggTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACggg                                                         >  2:1250843/1‑108 (MQ=255)
                                                                                                     gggTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACggg                                                         <  1:1250843/108‑1 (MQ=255)
                                                                                                     gggTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGc                                                        <  2:1136637/109‑1 (MQ=255)
                                                                                                     gggTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGc                                                        >  1:1136637/1‑109 (MQ=255)
                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGt                        >  2:468741/1‑139 (MQ=255)
                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGt                        >  2:504973/1‑139 (MQ=255)
                                                                                                         tAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTc                                 <  1:78938/128‑1 (MQ=255)
                                                                                                         tAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTc                                 >  2:78938/1‑128 (MQ=255)
                                                                                                         tAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTcc                      >  2:1635688/1‑139 (MQ=255)
                                                                                                                  ccACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  <  1:876533/54‑1 (MQ=255)
                                                                                                                  ccACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                  >  2:876533/1‑54 (MQ=255)
                                                                                                                     cGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGa                          <  1:940572/123‑1 (MQ=255)
                                                                                                                     cGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGa                          >  2:940572/1‑123 (MQ=255)
                                                                                                                       ccGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAAAAg                                                                                  >  1:794952/1‑65 (MQ=255)
                                                                                                                       ccGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAAAAg                                                                                  <  2:794952/65‑1 (MQ=255)
                                                                                                                             tCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAATATc  >  2:1447043/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                      
TCGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGCGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAATATC  >  NC_000913/3761721‑3761984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A