Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,878,603	T→C	D84G (GAT→GGT)	ygbF ←	CRISPR adaptation ssRNA endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,878,603	0	T	C	100.0%	158.3 / NA	46	D84G (GAT→GGT)	ygbF	CRISPR adaptation ssRNA endonuclease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/19); total (27/19)

TATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACGA  >  NC_000913/2878472‑2878737

tataCATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATc                                                                                                                                 >  1:1027616/1‑139 (MQ=255)

          ttCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTa                                                                                                                       >  2:671346/1‑139 (MQ=255)

          ttCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTa                                                                                                                       >  2:682998/1‑139 (MQ=255)

                          tCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCaa                                                                                                       <  2:1027616/139‑1 (MQ=255)

                                  aaaCAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTgg                                                                                               <  1:682998/139‑1 (MQ=255)

                                        cccACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCa                                                                                         <  1:438882/139‑1 (MQ=255)

                                        cccACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCa                                                                                         <  2:805861/139‑1 (MQ=255)

                                        cccACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCa                                                                                         >  1:805861/1‑139 (MQ=255)

                                                          aCATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTc                                                                       >  2:380460/1‑139 (MQ=255)

                                                               tCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAACTCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGc                                                                  >  2:259897/1‑139 (MQ=255)

                                                               tCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCgggg                                                                                                                         <  1:491756/84‑1 (MQ=255)

                                                               tCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCgggg                                                                                                                         >  2:491756/1‑84 (MQ=255)

                                                                ccTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTgg                                                                                               <  2:733119/109‑1 (MQ=255)

                                                                ccTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTgg                                                                                               >  1:733119/1‑109 (MQ=255)

                                                                ccTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGcc                                                                 >  1:983646/1‑139 (MQ=255)

                                                                ccTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGcc                                                                 >  1:693987/1‑139 (MQ=255)

                                                                ccTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGcc                                                                 >  1:486361/1‑139 (MQ=255)

                                                                    attattaAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATg                                                             >  2:417284/1‑139 (MQ=255)

                                                                     ttattaAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGc                                                            <  1:380460/139‑1 (MQ=255)

                                                                          aaaGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCa                                                       >  1:498974/1‑139 (MQ=255)

                                                                            aGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCaa                                                                                                       <  1:836314/89‑1 (MQ=255)

                                                                            aGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCaa                                                                                                       >  2:836314/1‑89 (MQ=255)

                                                                               tCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCgggg                                                                                                                         <  2:1019322/68‑1 (MQ=255)

                                                                               tCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCgggg                                                                                                                         >  1:1019322/1‑68 (MQ=255)

                                                                               tCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCa                                                       <  2:941292/134‑1 (MQ=255)

                                                                               tCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCa                                                       >  1:941292/1‑134 (MQ=255)

                                                                                cAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAATTCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGc                                                            <  1:1058657/128‑1 (MQ=255)

                                                                                cAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTAc                                                                                                                              <  1:957075/62‑1 (MQ=255)

                                                                                cAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTAc                                                                                                                              >  2:957075/1‑62 (MQ=255)

                                                                                cAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACAt                                                 >  1:82236/1‑139 (MQ=255)

                                                                                cAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTAACGGGGTACGCCTGTTTAACCCAACTGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGc                                                            >  2:1058657/1‑128 (MQ=255)

                                                                                        tCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTAc                                                                                                                              >  1:847918/1‑54 (MQ=255)

                                                                                        tCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTAc                                                                                                                              <  2:847918/54‑1 (MQ=255)

                                                                                           ttGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTcc                                      >  1:360195/1‑139 (MQ=255)

                                                                                            tGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCg                                     >  2:878065/1‑139 (MQ=255)

                                                                                            tGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCg                                     >  2:259238/1‑139 (MQ=255)

                                                                                             gTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGtt                                                                                <  2:225722/95‑1 (MQ=255)

                                                                                             gTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGtt                                                                                >  1:225722/1‑95 (MQ=255)

                                                                                                          tAAAAAAGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCAGCACTACATTGCCTTCTTCCGCCAGTCCAGCTAtt                       >  1:689591/1‑139 (MQ=255)

                                                                                                           aaaaaaGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTAttt                      <  2:82236/139‑1 (MQ=255)

                                                                                                             aaaaGACACCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGt                    <  2:486361/139‑1 (MQ=255)

                                                                                                                   cacCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCg                                                                      >  1:951401/1‑83 (MQ=255)

                                                                                                                   cacCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCg                                                                      <  2:951401/83‑1 (MQ=255)

                                                                                                                   cacCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGTTc                  >  1:354321/1‑135 (MQ=255)

                                                                                                                   cacCAACCTTAAACCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGTTc                  <  2:354321/135‑1 (MQ=255)

                                                                                                                              aaCCACCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACg   <  2:693987/139‑1 (MQ=255)

                                                                                                                               accaccCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACGa  >  2:410111/1‑139 (MQ=255)

TATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCCATCACTACATTGCCTTCTTCCGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACGA  >  NC_000913/2878472‑2878737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A