Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,929,455	C→T	N409N (AAC→AAT)	sdaC →	putative serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,929,455	0	C	T	100.0%	168.8 / NA	53	N409N (AAC→AAT)	sdaC	putative serine transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/25); total (28/25)

AGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGCGTATTC  >  NC_000913/2929333‑2929590

aGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTAtt                                                                                                                                    >  2:402545/1‑128 (MQ=255)

aGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTAtt                                                                                                                                    <  1:402545/128‑1 (MQ=255)

      cTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTc                                                                                                                   <  1:217488/139‑1 (MQ=255)

               aTTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                          >  2:460211/1‑123 (MQ=255)

               aTTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                          <  1:460211/123‑1 (MQ=255)

               aTTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCaa                                                                                                          >  1:776508/1‑139 (MQ=255)

                ttGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAAt                                                                                                         >  1:871910/1‑139 (MQ=255)

                   aaaCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAAtctc                                                                                                      >  1:959561/1‑139 (MQ=255)

                       ccTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  <  2:871910/139‑1 (MQ=255)

                         tGGGCGGTCCAATCATCGCGATGATTCTGTTGCGGATGCCGATGTACGCAATTCAGAAAGCATCGGCAATGCGTAAGTACAGCGGGCACATCAGCAATGTATTTGTTGTCGTGAGGGGTCTGTTTGCAATCTCAGCAAt                                                                                                <  2:104392/139‑1 (MQ=255)

                                tCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCACGTCAGAAAGTACCGGCCATGCGCAAGTACAGCAGTCACATCGGCAGTGTATTCGTTGTCGTGATGGGTCCGATTGCAATCTCCGCAATCTTCTAc                                                                                         >  2:867811/1‑139 (MQ=255)

                                 ccAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTAct                                                                                        <  1:667624/139‑1 (MQ=255)

                                          gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCaa                                                                                                          <  2:293411/112‑1 (MQ=255)

                                          gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCaa                                                                                                          >  1:293411/1‑112 (MQ=255)

                                              tgatCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTa                                                                           >  2:183659/1‑139 (MQ=255)

                                                          tGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgcg                                                               >  1:261663/1‑139 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  >  2:73893/1‑103 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  <  1:73893/103‑1 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGt                                                                                  <  2:1009913/119‑1 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGt                                                                                  >  1:1009913/1‑119 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCgcgc                                                              <  2:776508/139‑1 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCgcgc                                                              >  2:1065598/1‑139 (MQ=255)

                                                           gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCgcgc                                                              >  2:328414/1‑139 (MQ=255)

                                                            aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                                >  2:544100/1‑104 (MQ=255)

                                                            aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                                <  1:544100/104‑1 (MQ=255)

                                                                      aCGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGggc                                                   <  2:713282/139‑1 (MQ=255)

                                                                          aaTTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATg                                                                                                                       >  2:106561/1‑67 (MQ=255)

                                                                          aaTTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATg                                                                                                                       <  1:106561/67‑1 (MQ=255)

                                                                               aGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcc                                          >  2:470788/1‑139 (MQ=255)

                                                                                 aaaGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcctc                                        >  1:869677/1‑139 (MQ=255)

                                                                                      aCCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGttt                                   >  2:179602/1‑139 (MQ=255)

                                                                                       ccGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGtttt                                  <  1:328414/139‑1 (MQ=255)

                                                                                            aaTGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcgc                             <  1:168229/139‑1 (MQ=255)

                                                                                              tGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGa                           <  2:869677/139‑1 (MQ=255)

                                                                                               gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGAt                          <  1:1065598/139‑1 (MQ=255)

                                                                                                   aaGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt                      <  1:183659/139‑1 (MQ=255)

                                                                                                      tACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTc                   >  2:698849/1‑139 (MQ=255)

                                                                                                        cAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt                      >  2:277936/1‑134 (MQ=255)

                                                                                                        cAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt                      <  1:277936/134‑1 (MQ=255)

                                                                                                          gCGGTCACATCAGCATTGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTAt               >  2:752306/1‑139 (MQ=255)

                                                                                                          gCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAtctt                                                                                             >  2:251861/1‑61 (MQ=255)

                                                                                                          gCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAtctt                                                                                             <  1:251861/61‑1 (MQ=255)

                                                                                                           cGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATg              >  2:673079/1‑139 (MQ=255)

                                                                                                             gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                                    <  1:11271/51‑1 (MQ=255)

                                                                                                             gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                                    >  2:11271/1‑51 (MQ=255)

                                                                                                             gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  <  2:303143/53‑1 (MQ=255)

                                                                                                             gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  >  1:303143/1‑53 (MQ=255)

                                                                                                                acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcct                                         <  1:676087/107‑1 (MQ=255)

                                                                                                                acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcct                                         >  2:676087/1‑107 (MQ=255)

                                                                                                                acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcg                              <  1:416058/118‑1 (MQ=255)

                                                                                                                acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcg                              >  2:416058/1‑118 (MQ=255)

                                                                                                                 caTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGc        <  2:959561/139‑1 (MQ=255)

                                                                                                                       cAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGCGTATTc  >  2:964732/1‑139 (MQ=255)

AGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGCGTATTC  >  NC_000913/2929333‑2929590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A