Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,980,957 |
(G)6→8 |
coding (71/1386 nt) |
yifK → |
putative APC family amino acid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,980,951 | 1 | . | G | 100.0%
| 149.9
/ NA
| 42 | L22R (CTG→CGG) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (23/19); total (23/19) |
| * | NC_000913 | 3,980,951 | 2 | . | G | 100.0%
| 149.1
/ NA
| 42 | L22R (CTG→CGG) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (23/19); total (23/19) |
CTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGA > NC_000913/3980824‑3981080
||
cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑ggggggggcac > 1:833654/1‑134 (MQ=255)
aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCg > 1:728639/1‑139 (MQ=255)
aCCCATAACGATAACCGGTAACACAGGAAAGCATGCAAACACAACACGAGGATTTATGGCACCTACCAAACCAGCGCTGCAGTTTGGGCTGGAAGCTCGACCTATCGCACTCACCGCCAT‑‑‑GGGGGGGACACCATGGGCg > 2:850299/1‑139 (MQ=255)
tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGc > 1:390801/1‑139 (MQ=255)
tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGc > 2:763567/1‑139 (MQ=255)
aaCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGcc > 1:88147/1‑139 (MQ=255)
aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCt > 2:583808/1‑139 (MQ=255)
aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTAt > 2:1047807/1‑139 (MQ=255)
aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGgc < 2:728639/139‑1 (MQ=255)
aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGgc > 1:797235/1‑139 (MQ=255)
cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:1047807/139‑1 (MQ=255)
aGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 2:833654/139‑1 (MQ=255)
aGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 2:217982/139‑1 (MQ=255)
cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCt > 2:1064524/1‑139 (MQ=255)
aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTg > 2:144916/1‑139 (MQ=255)
cAAACACAACACGAGGATTTATGGCAGATAACAAACCCGAGCTACAGCGTGGGCTGGAAGCTCTACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCGTGaaa < 2:916250/139‑1 (MQ=255)
caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑ggg < 1:791433/82‑1 (MQ=255)
caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑ggg > 2:791433/1‑82 (MQ=255)
cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa < 1:942364/129‑1 (MQ=255)
cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa > 2:423315/1‑129 (MQ=255)
cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa > 2:942364/1‑129 (MQ=255)
cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa < 1:423315/129‑1 (MQ=255)
acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 2:88147/139‑1 (MQ=255)
aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 1:107743/1‑106 (MQ=255)
aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 2:107743/106‑1 (MQ=255)
tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 1:1064524/139‑1 (MQ=255)
tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 1:144916/139‑1 (MQ=255)
cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg < 2:797235/139‑1 (MQ=255)
gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc > 1:650400/1‑139 (MQ=255)
gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:22733/90‑1 (MQ=255)
gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:22733/1‑90 (MQ=255)
agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGcc > 1:892144/1‑139 (MQ=255)
gCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg < 2:390801/139‑1 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGcc < 1:530207/61‑1 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGcc > 2:530207/1‑61 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCg > 1:501230/1‑139 (MQ=255)
ggCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑gggggggg > 2:298330/1‑40 (MQ=255)
ggCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑gggggggg < 1:298330/42‑3 (MQ=255)
gCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat < 1:763567/139‑1 (MQ=255)
gCTCGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat < 1:583808/139‑1 (MQ=255)
cGACATATCGAACTCATCGCCCTGGGGGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAGTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatca > 1:1029743/1‑139 (MQ=255)
cGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc < 2:532201/88‑1 (MQ=255)
cGACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc > 1:532201/1‑88 (MQ=255)
gACATATCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATg < 1:211363/139‑1 (MQ=255)
atCGAACTCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTc > 1:465220/1‑139 (MQ=255)
tCATCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGc > 1:247136/1‑139 (MQ=255)
aTCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGa > 2:1033205/1‑139 (MQ=255)
aTCGCCCTGG‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGa > 1:767690/1‑139 (MQ=255)
||
CTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑‑GGGGGG‑‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGA > NC_000913/3980824‑3981080
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
