Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,694,840 G→A H133Y (CAT→TAT)  bcsA ← cellulose synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,694,8400GA100.0% 211.4 / NA 65H133Y (CAT→TAT) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/32);  total (33/32)

CGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694712‑3694971
                                                                                                                                |                                                                                                                                   
cgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTa                                                                                                                           >  2:1792960/1‑139 (MQ=255)
cgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTa                                                                                                                           >  2:1206751/1‑139 (MQ=255)
 gcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTAt                                                                                                                          >  1:404768/1‑139 (MQ=255)
  ccAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATg                                                                                                                         >  1:1357040/1‑139 (MQ=255)
       gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:1029706/139‑1 (MQ=255)
       gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:1357040/139‑1 (MQ=255)
       gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:1206751/139‑1 (MQ=255)
          ttAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAcgc                                                                                                                 >  1:1793157/1‑139 (MQ=255)
           tAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAcgcg                                                                                                                <  1:976559/139‑1 (MQ=255)
                  cTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGc                                                                                                         <  2:404768/139‑1 (MQ=255)
                  cTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGc                                                                                                         <  1:81153/139‑1 (MQ=255)
                     aGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGc                                                                                                      >  1:1101243/1‑139 (MQ=255)
                        aaCGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCg                                                                                                   <  1:1191304/139‑1 (MQ=255)
                               aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:845679/115‑1 (MQ=255)
                               aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  2:845679/1‑115 (MQ=255)
                                 tAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGATGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAt                                                                                          <  2:802897/139‑1 (MQ=255)
                                    cGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAcgcg                                                                                                                <  2:740290/114‑1 (MQ=255)
                                    cGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAcgcg                                                                                                                >  1:740290/1‑114 (MQ=255)
                                         gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTa                                                                                  >  2:1254155/1‑139 (MQ=255)
                                                      ggTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAata                                                                     <  1:1792960/139‑1 (MQ=255)
                                                          aCGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATAtttt                                                                 >  2:739770/1‑139 (MQ=255)
                                                                 aaCCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGAcc                                                          >  1:1545668/2‑139 (MQ=255)
                                                                      aGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGaaa                                                     >  2:773971/1‑139 (MQ=255)
                                                                             aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATc                                                                                             <  2:255299/92‑1 (MQ=255)
                                                                             aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATc                                                                                             >  1:255299/1‑92 (MQ=255)
                                                                             aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAt                                                                                          >  1:1146141/1‑95 (MQ=255)
                                                                             aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAt                                                                                          <  2:1146141/95‑1 (MQ=255)
                                                                             aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCg                                              >  1:849401/1‑139 (MQ=255)
                                                                             aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCg                                              >  2:602255/1‑139 (MQ=255)
                                                                                  cgcgCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCa                                                              <  1:400550/118‑1 (MQ=255)
                                                                                  cgcgCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCa                                                              >  2:400550/1‑118 (MQ=255)
                                                                                  cacgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTg                                                                                        >  1:1008321/3‑92 (MQ=255)
                                                                                  cacgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTg                                                                                        <  2:1008321/90‑1 (MQ=255)
                                                                                   gcgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgcg                                        <  2:1793157/139‑1 (MQ=255)
                                                                                    cgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGc                                                               <  2:1408009/115‑1 (MQ=255)
                                                                                    cgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGc                                                               >  1:1408009/1‑115 (MQ=255)
                                                                                            cACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCgg                               >  2:1292143/1‑139 (MQ=255)
                                                                                               tCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGAACTGAAAATGCCCGCCTGCGCGGTTTCGGCGt                            >  1:978531/1‑139 (MQ=255)
                                                                                                 aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:824190/49‑1 (MQ=255)
                                                                                                 aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  2:824190/1‑49 (MQ=255)
                                                                                                 aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:469142/49‑1 (MQ=255)
                                                                                                 aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  2:469142/1‑49 (MQ=255)
                                                                                                 aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAtt                                                                                         <  2:1700222/76‑1 (MQ=255)
                                                                                                 aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAtt                                                                                         >  1:1700222/1‑76 (MQ=255)
                                                                                                     gACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTa                                                                                  <  2:999781/79‑1 (MQ=255)
                                                                                                     gACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTa                                                                                  >  1:999781/1‑79 (MQ=255)
                                                                                                     aagagacagctTTTTTCTCACTCAGATACTGTGGTTTATGGCTAACGCGCTCAGGCACCTCGCTCATCCATCGATGGTAACGTCCACGAATAATTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCttt                      <  1:1096740/130‑1 (MQ=255)
                                                                                                         gAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcgc                  >  1:741448/1‑139 (MQ=255)
                                                                                                           gCTCTTTTTTCTCATCCAGATACTGTGTTTTATgg                                                                                                                        >  1:956939/1‑35 (MQ=255)
                                                                                                           gCTCTTTTTTCTCATCCAGATACTGTGTTTTATgg                                                                                                                        <  2:956939/35‑1 (MQ=255)
                                                                                                           gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGa                >  1:24033/1‑139 (MQ=255)
                                                                                                             tctTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGa                                                                        <  2:481394/81‑1 (MQ=255)
                                                                                                             tctTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGa                                                                        >  1:481394/1‑81 (MQ=255)
                                                                                                               ttttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 >  1:1377968/1‑54 (MQ=255)
                                                                                                               ttttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 <  1:1036821/54‑1 (MQ=255)
                                                                                                               ttttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 <  2:1377968/54‑1 (MQ=255)
                                                                                                               ttttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 >  2:1036821/1‑54 (MQ=255)
                                                                                                               ttttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCCTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTCTCTTTGCGCGATGCa            <  1:1254155/139‑1 (MQ=255)
                                                                                                                 ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  2:24033/139‑1 (MQ=255)
                                                                                                                 ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  1:602255/139‑1 (MQ=255)
                                                                                                                     ctcATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATc      <  1:773971/139‑1 (MQ=255)
                                                                                                                       cATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  1:94826/133‑1 (MQ=255)
                                                                                                                       cATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          >  2:94826/1‑133 (MQ=255)
                                                                                                                        aTCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAAt   <  2:1101243/139‑1 (MQ=255)
                                                                                                                         tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcctgtctctt  >  2:1096740/1‑131 (MQ=255)
                                                                                                                                |                                                                                                                                   
CGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694712‑3694971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A