Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,416	G→A	219 (TAG→TAA)	pphB →	serine/threonine‑specific protein phosphatase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,416	0	G	A	100.0%	140.3 / NA	44	219 (TAG→TAA)	pphB	serine/threonine‑specific protein phosphatase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/19); total (25/19)

CAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAA  >  NC_000913/2860285‑2860552

caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                   <  2:740893/139‑1 (MQ=255)

       aCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCCCATGC‑tcttct                                                                                                                           >  2:821621/1‑139 (MQ=255)

                     tttATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTa                                                                                                             >  2:1233993/1‑139 (MQ=255)

                         tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                         <  1:1233993/139‑1 (MQ=255)

                         tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                         <  2:1206234/139‑1 (MQ=255)

                          atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAg                                                                                                               <  2:1438724/132‑1 (MQ=255)

                          atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAg                                                                                                               >  1:1438724/1‑132 (MQ=255)

                          atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCg                                                                                                        >  1:1211120/1‑139 (MQ=255)

                              ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                   <  2:947632/109‑1 (MQ=255)

                              ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                   >  1:947632/1‑109 (MQ=255)

                              ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGc                                                                                                                    <  2:167953/123‑1 (MQ=255)

                              ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGc                                                                                                                    >  1:167953/1‑123 (MQ=255)

                                   tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                               >  2:968054/1‑139 (MQ=255)

                                                        gACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCg                                                                          >  2:675339/1‑139 (MQ=255)

                                                            ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                      >  1:650241/1‑139 (MQ=255)

                                                                   aCCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCaaa                                                               >  2:1181765/1‑139 (MQ=255)

                                                                        aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                          >  2:1409585/1‑139 (MQ=255)

                                                                        aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                          >  1:902053/1‑139 (MQ=255)

                                                                          ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                        <  1:1409585/139‑1 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAcc                                                                                                          <  2:382618/81‑1 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAcc                                                                                                          >  1:382618/1‑81 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                       <  1:710167/116‑1 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                       >  2:710167/1‑116 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCa                                                                 >  2:1434239/1‑122 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCa                                                                 <  1:1434239/122‑1 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                >  1:916700/1‑139 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                >  2:1352068/1‑139 (MQ=255)

                                                                                        gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgc                                          >  2:951566/1‑139 (MQ=255)

                                                                                            gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAAtc                                                                                                                                        <  2:752839/42‑1 (MQ=255)

                                                                                            gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                   <  2:1270327/47‑1 (MQ=255)

                                                                                            gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                   >  1:1270327/1‑47 (MQ=255)

                                                                                            gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                       <  2:820840/106‑1 (MQ=255)

                                                                                            gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                       >  1:820840/1‑106 (MQ=255)

                                                                                             ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGcgcgcg                                     >  1:584795/1‑139 (MQ=255)

                                                                                             ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGcgcgcg                                     >  2:28264/1‑139 (MQ=255)

                                                                                                aaCAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACt                                  >  1:1402993/1‑139 (MQ=255)

                                                                                                    gcgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                              <  1:1352068/139‑1 (MQ=255)

                                                                                                       ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTgg                                <  1:1246193/134‑1 (MQ=255)

                                                                                                       ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTgg                                >  2:1246193/1‑134 (MQ=255)

                                                                                                                     tataAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtc             <  2:1402993/139‑1 (MQ=255)

                                                                                                                     tataAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTAGCACTCTCCGGCAAACcgtc             <  1:821621/139‑1 (MQ=255)

                                                                                                                       taaaataaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                       >  1:135284/1‑79 (MQ=255)

                                                                                                                       taaaataaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                       <  2:135284/79‑1 (MQ=255)

                                                                                                                       taaaataaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt           <  1:951566/139‑1 (MQ=255)

                                                                                                                               agtaatCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                      >  2:1294865/1‑72 (MQ=255)

                                                                                                                               agtaatCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                      <  1:1294865/72‑1 (MQ=255)

                                                                                                                                gtaatCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaataa  <  1:675339/139‑1 (MQ=255)

CAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAA  >  NC_000913/2860285‑2860552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A