Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,015	G→A	intergenic (‑105/‑100)	nudF ← / → tolC	ADP‑ribose pyrophosphatase/transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,015	0	G	A	100.0%	161.7 / NA	51	intergenic (‑105/‑100)	nudF/tolC	ADP‑ribose pyrophosphatase/transport channel
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/24); total (27/24)

GGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAG  >  NC_000913/3177888‑3178149

ggCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGcc                                                                                                                             <  1:1070505/139‑1 (MQ=255)

  cAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCaa                                                                                                                           >  1:23875/1‑139 (MQ=255)

    ggTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAAcc                                                                                                                         >  1:882997/1‑139 (MQ=255)

       tGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCttt                                                                                                                      <  2:882997/139‑1 (MQ=255)

        gTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCtttt                                                                                                                     >  1:1189592/1‑139 (MQ=255)

               ttAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAg                                                                                                              <  2:1189592/139‑1 (MQ=255)

                 aaGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACTTTTTGCGGTAGCg                                                                                                            <  2:1030023/139‑1 (MQ=255)

                 aaGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCg                                                                                                            <  2:333173/139‑1 (MQ=255)

                  aGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCgg                                                                                                           >  1:564052/1‑139 (MQ=255)

                   gCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  1:586652/139‑1 (MQ=255)

                   gCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:23875/139‑1 (MQ=255)

                       tGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGcc                                                                                                                             >  1:201844/1‑116 (MQ=255)

                       tGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGcc                                                                                                                             <  2:201844/116‑1 (MQ=255)

                       tGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCa                                                                                                                            <  2:1141662/117‑1 (MQ=255)

                       tGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCa                                                                                                                            >  1:1141662/1‑117 (MQ=255)

                                 cTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATcc                                                                                            >  2:475989/1‑139 (MQ=255)

                                   ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  2:836683/1‑139 (MQ=255)

                                   ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  1:587436/1‑139 (MQ=255)

                                   ggCACATATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTTATGTGTTATATTGGGGCCCATAAAGGCCCTATGACAGTTATCTCCATCTTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  2:1062898/1‑139 (MQ=255)

                                        tAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaataa                                                                                     >  1:1495052/1‑139 (MQ=255)

                                         aaTAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaataat                                                                                    >  1:20157/1‑139 (MQ=255)

                                           tAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAAttt                                                                                  <  1:475989/139‑1 (MQ=255)

                                             gTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTa                                                                                <  2:1495052/139‑1 (MQ=255)

                                              tGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTAc                                                                               >  2:556973/1‑139 (MQ=255)

                                                aaTTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAg                                                                             >  1:738306/1‑139 (MQ=255)

                                                     aaTGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGa                                                                        <  2:1067841/139‑1 (MQ=255)

                                                       tgtgAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      <  1:1462314/139‑1 (MQ=255)

                                                       tgtgAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      <  2:20157/139‑1 (MQ=255)

                                                          gAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATcgcg                                                                   >  2:957934/1‑139 (MQ=255)

                                                                 aGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATa                                                            >  1:65158/1‑139 (MQ=255)

                                                                      cGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCt                                                       <  1:957934/139‑1 (MQ=255)

                                                                        tttGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTc                                                     <  1:836683/139‑1 (MQ=255)

                                                                            aCTGCCGTTTGAGCAGTAATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTcacca                                                 <  1:404745/139‑1 (MQ=255)

                                                                                ccGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAg                                             <  2:738306/139‑1 (MQ=255)

                                                                                ccGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACAACAAg                                             >  1:1137948/1‑139 (MQ=255)

                                                                                          aGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTg                                                                                                                    <  2:813065/58‑1 (MQ=255)

                                                                                          aGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTg                                                                                                                    >  1:813065/1‑58 (MQ=255)

                                                                                             cATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaataa                                                                                     >  1:42309/1‑86 (MQ=255)

                                                                                             cATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaataa                                                                                     <  2:42309/86‑1 (MQ=255)

                                                                                                      aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  1:598483/56‑1 (MQ=255)

                                                                                                      aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  2:598483/1‑56 (MQ=255)

                                                                                                      aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAAttt                                                                                  <  1:347406/80‑1 (MQ=255)

                                                                                                      aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAAttt                                                                                  >  2:347406/1‑80 (MQ=255)

                                                                                                      aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      <  2:287297/92‑1 (MQ=255)

                                                                                                      aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      >  1:287297/1‑92 (MQ=255)

                                                                                                        ttGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCt                                                                 <  2:808162/95‑1 (MQ=255)

                                                                                                        ttGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCt                                                                 >  1:808162/1‑95 (MQ=255)

                                                                                                                caTTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCtt             >  1:334330/1‑139 (MQ=255)

                                                                                                                  ttAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTAt           >  2:793558/1‑139 (MQ=255)

                                                                                                                           tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAg  >  2:1435022/1‑139 (MQ=255)

                                                                                                                           tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAg  >  2:342131/1‑139 (MQ=255)

GGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAG  >  NC_000913/3177888‑3178149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A