Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,269,602 |
G→A |
*396* (TAG→TAA) |
yhaC → |
pentapetide repeats‑related protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,269,602 | 0 | G | A | 96.7%
| 86.7
/ ‑3.4
| 30 | *396* (TAG→TAA) | yhaC | pentapetide repeats‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/12); minor base T (1/0); total (18/12) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01 |
TCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTA > NC_000913/3269466‑3269734
|
tCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAAc > 2:925178/1‑139 (MQ=255)
ttCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa < 2:48730/139‑1 (MQ=255)
ccGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt < 1:1079820/139‑1 (MQ=255)
acacATATGAAGCATATAACTCAGAGAAGCAATATATTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATc < 1:925178/139‑1 (MQ=255)
acaTATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCtt > 1:1412894/1‑139 (MQ=255)
atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa < 1:1295527/98‑1 (MQ=255)
atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa > 2:1295527/1‑98 (MQ=255)
atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt < 1:1288681/102‑1 (MQ=255)
atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt > 2:1288681/1‑102 (MQ=255)
atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAccc > 2:1060055/1‑139 (MQ=255)
tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCAcc > 2:963932/1‑139 (MQ=255)
cTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTaa < 1:1525386/99‑1 (MQ=255)
cTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTaa > 2:1525386/1‑99 (MQ=255)
agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATATACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaa > 1:214539/1‑139 (MQ=255)
agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc > 1:1159617/1‑122 (MQ=255)
agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc < 2:1159617/122‑1 (MQ=255)
gagaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaa > 1:329866/1‑139 (MQ=255)
agaAGCAATATTTTACCAGAGAGGCCTATACGTATGATTACGACCTTTTATACGCAATATAAACTTAATAACTTCTTACAAAGCCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCacaa > 1:769717/1‑139 (MQ=255)
gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaa < 1:486178/63‑1 (MQ=255)
gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaa > 2:486178/1‑63 (MQ=255)
gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc < 2:1206989/102‑1 (MQ=255)
gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc > 1:1206989/1‑102 (MQ=255)
taCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt > 2:738916/1‑56 (MQ=255)
taCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt < 1:738916/56‑1 (MQ=255)
aTTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGc > 2:596926/1‑139 (MQ=255)
tACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc < 1:273821/82‑1 (MQ=255)
tACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc > 2:273821/1‑82 (MQ=255)
tttAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGat > 1:743690/1‑58 (MQ=255)
tttAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGat < 2:743690/58‑1 (MQ=255)
aaTATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTa > 2:481181/1‑139 (MQ=255)
|
TCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTA > NC_000913/3269466‑3269734
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
