Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,994	(C)_6→7	coding (567/1125 nt)	smf ←	DNA recombination‑mediator A family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,988	1	.	C	98.5%	228.6 / ‑3.9	65	coding (573/1125 nt)	smf	DNA recombination‑mediator A family protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (35/29); total (35/30)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGC  >  NC_000913/3432850‑3433105

tGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg                                                                                                                         >  2:122792/1‑139 (MQ=255)

 gCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                       <  2:1278574/139‑1 (MQ=255)

 gCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                       <  1:92091/139‑1 (MQ=255)

      cACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                                 >  1:532101/1‑139 (MQ=255)

          aGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑ccctgt                                                                                                             <  1:1150391/139‑5 (MQ=255)

          aGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                                 <  1:1172429/135‑1 (MQ=255)

          aGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                                 >  2:1172429/1‑135 (MQ=255)

               aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAg                                                                                                         >  2:978669/1‑139 (MQ=255)

               aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAg                                                                                                         >  2:40228/1‑139 (MQ=255)

                   cTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGa                                                                                                     >  2:677721/1‑139 (MQ=255)

                     aCGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑c                                                                                                                  >  2:1251376/1‑123 (MQ=255)

                     aCGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑c                                                                                                                  <  1:1251376/123‑1 (MQ=255)

                         aaaGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCa                                                                                               >  1:400777/1‑139 (MQ=255)

                           aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATGGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACt                                                                                                   >  1:1015803/1‑133 (MQ=255)

                           aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGCGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACt                                                                                                   <  2:1015803/133‑1 (MQ=255)

                            gCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGcc                                                                                            <  1:677721/139‑1 (MQ=255)

                             ccGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCa                                                                                           >  1:1261948/1‑139 (MQ=255)

                               gCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGt                                                                                         <  1:122792/139‑1 (MQ=255)

                                cTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTc                                                                                        >  1:1134565/1‑139 (MQ=255)

                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGa                                                                                                     >  2:5072/1‑121 (MQ=255)

                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGa                                                                                                     <  1:5072/121‑1 (MQ=255)

                                           aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                             <  2:337943/139‑1 (MQ=255)

                                             tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                           >  1:308254/1‑139 (MQ=255)

                                             tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                           >  1:741880/1‑139 (MQ=255)

                                                 ccTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGa                                                                       >  2:243284/1‑139 (MQ=255)

                                                  cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                      >  1:436032/1‑139 (MQ=255)

                                                  cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                      <  2:532101/139‑1 (MQ=255)

                                                      aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  <  2:1503404/139‑1 (MQ=255)

                                                      aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  <  2:1261948/139‑1 (MQ=255)

                                                       cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATg                                                                 <  1:40228/139‑1 (MQ=255)

                                                         tAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                       >  2:1415080/1‑83 (MQ=255)

                                                         tAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                       <  1:1415080/83‑1 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGc                                                                                             <  1:1087528/106‑1 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAtt                                                            >  2:1297443/1‑139 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAtt                                                            >  2:1439242/1‑139 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                                 >  1:654116/1‑85 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                                 <  2:654116/85‑1 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGATGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGc                                                                                             >  2:1087528/1‑106 (MQ=255)

                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAAATGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCGAGTCGGGCATGACGGCGGGGATGAATTGTaat                                                            >  2:500365/1‑137 (MQ=255)

                                                              cACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa                                                          <  2:741880/139‑1 (MQ=255)

                                                                   aTAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:1439242/139‑1 (MQ=255)

                                                                   aTAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:243284/139‑1 (MQ=255)

                                                                     aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCAtt                                                   >  2:68190/1‑139 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAg                                                                                          <  2:539567/94‑1 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAg                                                                                          >  1:539567/1‑94 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  <  2:479523/118‑1 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  >  1:479523/1‑118 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     >  2:722765/1‑131 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:722765/131‑1 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                <  1:207741/136‑1 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                >  2:207741/1‑136 (MQ=255)

                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                             <  1:932161/139‑1 (MQ=255)

                                                                                 tcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGc                                       >  1:1418236/1‑139 (MQ=255)

                                                                                       gAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCt                                 <  2:400777/139‑1 (MQ=255)

                                                                                         aaTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGa                               <  2:1418236/139‑1 (MQ=255)

                                                                                          aTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGAc                              >  2:1242538/1‑139 (MQ=255)

                                                                                           ttGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                             <  1:500365/139‑1 (MQ=255)

                                                                                              tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCa                          <  2:436032/139‑1 (MQ=255)

                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                   >  1:1246288/1‑96 (MQ=255)

                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                   <  2:1246288/96‑1 (MQ=255)

                                                                                                    aGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTAcc                    >  1:485576/1‑139 (MQ=255)

                                                                                                      gggTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                       >  2:797265/1‑38 (MQ=255)

                                                                                                      gggTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                       <  1:797265/38‑1 (MQ=255)

                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  2:606670/94‑1 (MQ=255)

                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     >  1:606670/1‑94 (MQ=255)

                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATCAAGTCCATTCCCCAATACAGCAATGCTGACg                             >  1:98396/1‑118 (MQ=255)

                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCG‑CCCCACCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                             <  2:98396/118‑1 (MQ=255)

                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:687754/93‑1 (MQ=255)

                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     >  2:687754/1‑93 (MQ=255)

                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa                <  2:404102/130‑1 (MQ=255)

                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa                >  1:404102/1‑130 (MQ=255)

                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt       >  1:510891/1‑139 (MQ=255)

                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt       >  2:310979/1‑139 (MQ=255)

                                                                                                                  gAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt      >  2:253330/1‑139 (MQ=255)

                                                                                                                   aGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                  >  2:1169183/1‑94 (MQ=255)

                                                                                                                   aGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                  <  1:1169183/94‑1 (MQ=255)

                                                                                                                   aGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTa     >  1:154596/1‑139 (MQ=255)

                                                                                                                      ggAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATgc  <  2:154596/139‑1 (MQ=255)

                                                                                                                            tCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                       >  2:40226/1‑112 (MQ=255)

                                                                                                                            tCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                       <  1:40226/112‑1 (MQ=255)

TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGC  >  NC_000913/3432850‑3433105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A