Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,842,455 C→T *445* (TAG→TAA)  adeQ ← adenine permease, high affinity, adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,842,4550CT100.0% 225.8 / NA 70*445* (TAG→TAA) adeQadenine permease, high affinity, adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

CCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTT  >  NC_000913/3842349‑3842582
                                                                                                          |                                                                                                                               
ccGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACag                                                                                                 >  1:839311/1‑139 (MQ=255)
          accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacc                                                                                       >  2:418855/1‑139 (MQ=255)
                     tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                            <  1:988996/91‑1 (MQ=255)
                     tACAGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                            >  2:988996/1‑91 (MQ=255)
                       ccGCCAGCGTGGCGAGCATTGTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGACCTGCGACCACCACcacaca                                                                            >  2:901019/1‑137 (MQ=255)
                       ccGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       <  2:250346/94‑1 (MQ=255)
                       ccGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       >  1:250346/1‑94 (MQ=255)
                       ccGCCAGCGTGGCGAGCATCATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  1:901019/137‑1 (MQ=255)
                                            tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCGTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     >  1:149094/1‑139 (MQ=255)
                                            tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     <  1:418855/139‑1 (MQ=255)
                                                 aTCATTAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTTCGACCAGCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                                <  2:1384066/139‑1 (MQ=255)
                                                 aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCTTCGCCAGTTATTAATccac                                                                                                                         <  2:1111275/66‑1 (MQ=255)
                                                 aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCTTCGCCAGTTATTAATccac                                                                                                                         >  1:1111275/1‑66 (MQ=255)
                                                 aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTg                                                                                            <  2:681899/95‑1 (MQ=255)
                                                 aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTg                                                                                            >  1:681899/1‑95 (MQ=255)
                                                 aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:350324/1‑111 (MQ=255)
                                                 aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:350324/111‑1 (MQ=255)
                                                           ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   <  1:1459454/94‑1 (MQ=255)
                                                           ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   >  2:1459454/1‑94 (MQ=255)
                                                           ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     >  1:877221/1‑124 (MQ=255)
                                                           ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     <  2:877221/124‑1 (MQ=255)
                                                           ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                      >  1:1151269/1‑139 (MQ=255)
                                                                   tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                 <  2:1120969/136‑1 (MQ=255)
                                                                   tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                 >  1:1120969/1‑136 (MQ=255)
                                                                   tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAg                              >  2:143463/1‑139 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       >  1:314173/1‑47 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                       <  2:314173/47‑1 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                      <  2:780172/80‑1 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                      >  1:780172/1‑80 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:889024/90‑1 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:889024/1‑90 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGGGCCAGCGCCCGGTGCCTACTTTCATGATGCAGTAc                           >  2:1156499/1‑139 (MQ=255)
                                                                      tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTAc                           >  2:1318438/1‑139 (MQ=255)
                                                                           aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACAt                      <  2:1151269/139‑1 (MQ=255)
                                                                             tCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:618088/83‑1 (MQ=255)
                                                                             tCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:618088/1‑83 (MQ=255)
                                                                               aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcg                                                               <  1:1129506/94‑1 (MQ=255)
                                                                               aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcg                                                               >  2:1129506/1‑94 (MQ=255)
                                                                                tGATCTCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcataca                                                                            <  2:987320/80‑1 (MQ=255)
                                                                                tGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:987320/1‑80 (MQ=255)
                                                                                    atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAg             >  1:1125506/1‑139 (MQ=255)
                                                                                    atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAg             >  2:423583/1‑139 (MQ=255)
                                                                                       tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                           <  2:512277/106‑1 (MQ=255)
                                                                                       tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                           >  1:512277/1‑106 (MQ=255)
                                                                                       tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc          >  2:371335/1‑139 (MQ=255)
                                                                                        ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa         <  2:149094/139‑1 (MQ=255)
                                                                                         gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                           >  1:1077812/1‑72 (MQ=255)
                                                                                         gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                           <  2:1077812/72‑1 (MQ=255)
                                                                                         gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGtt                                                                      <  1:1095424/77‑1 (MQ=255)
                                                                                         gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGtt                                                                      >  2:1095424/1‑77 (MQ=255)
                                                                                         gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa        <  1:143463/139‑1 (MQ=255)
                                                                                         gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa        <  2:1125506/139‑1 (MQ=255)
                                                                                          aaaCCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAAt       >  1:1171937/1‑139 (MQ=255)
                                                                                               cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCGAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTACAATCCCtt  >  2:907247/1‑139 (MQ=255)
                                                                                                 cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                        <  2:377480/51‑1 (MQ=255)
                                                                                                 cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                        >  1:377480/1‑51 (MQ=255)
                                                                                                 cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  2:1149165/1‑63 (MQ=255)
                                                                                                 cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            >  1:497207/1‑63 (MQ=255)
                                                                                                 cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  2:497207/63‑1 (MQ=255)
                                                                                                 cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                            <  1:1149165/63‑1 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   <  1:507374/54‑1 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                   >  2:507374/1‑54 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     <  2:532142/84‑1 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                     >  1:532142/1‑84 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                              >  1:690881/1‑91 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                              <  2:690881/91‑1 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                            >  1:761574/1‑109 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                            <  2:761574/109‑1 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCg               <  1:1192098/122‑1 (MQ=255)
                                                                                                   ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCg               >  2:1192098/1‑122 (MQ=255)
                                                                                                          |                                                                                                                               
CCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTT  >  NC_000913/3842349‑3842582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A