Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,386	C→T	R78C (CGT→TGT)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,386	0	C	T	100.0%	114.4 / NA	37	R78C (CGT→TGT)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/16); total (21/16)

GCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTA  >  NC_000913/3927254‑3927520

gcgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGa                                                                                                                                  >  1:1140951/1‑139 (MQ=255)

       ttCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAgg                                                                                                                           >  2:797386/1‑139 (MQ=255)

                 tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGAc                                                                                                                 >  2:123887/1‑139 (MQ=255)

                       ggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgc                                                                                                           <  2:1140951/139‑1 (MQ=255)

                             cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGc                                                                                                     >  1:1358861/1‑139 (MQ=255)

                                 cAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAg                                                                                                 <  1:797386/139‑1 (MQ=255)

                                   ggATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGACGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAggg                                                                                               <  2:524205/139‑1 (MQ=255)

                                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGc                                                                                                                         >  1:449026/1‑102 (MQ=255)

                                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGc                                                                                                                         <  2:449026/102‑1 (MQ=255)

                                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                                          <  2:821899/117‑1 (MQ=255)

                                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                                          >  1:821899/1‑117 (MQ=255)

                                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAAGGCAACACGACTTCAGCGCGGGCGa                                                                                                   >  2:58952/1‑124 (MQ=255)

                                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAAGGCAACACGACTTCAGCGCGGGCGa                                                                                                   <  1:58952/124‑1 (MQ=255)

                                                                              aaaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGCAGTGGCAATGTCAAACCTTAGGGCAACACATCTTCAGCGCGGGCGAAGGGATGTACACGCACATGAAAGCCCTTCGCCCCGATTAAGACCGTc                                                    >  2:598222/1‑139 (MQ=255)

                                                                               aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCt                                                   >  2:251919/1‑139 (MQ=255)

                                                                                     tGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTcc                                             >  1:400272/1‑139 (MQ=255)

                                                                                           aTGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCa                                       >  1:1155544/1‑139 (MQ=255)

                                                                                            tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                      <  2:709434/139‑1 (MQ=255)

                                                                                                cAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                  >  2:557820/1‑139 (MQ=255)

                                                                                                cAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCGTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                  <  2:717955/139‑1 (MQ=255)

                                                                                                 aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAaca                                                                                                                     <  2:1003710/55‑1 (MQ=255)

                                                                                                 aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAaca                                                                                                                     >  1:1003710/1‑55 (MQ=255)

                                                                                                 aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                 <  1:557820/139‑1 (MQ=255)

                                                                                                   ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              >  1:1011073/1‑92 (MQ=255)

                                                                                                   ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCt                               >  2:646440/1‑139 (MQ=255)

                                                                                                   ttCGAAGAGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              <  2:1011073/92‑1 (MQ=255)

                                                                                                        aGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGa                                                                                                   >  1:695604/1‑66 (MQ=255)

                                                                                                        aGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGa                                                                                                   <  2:695604/66‑1 (MQ=255)

                                                                                                               cattcaCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              >  2:141427/1‑80 (MQ=255)

                                                                                                               cattcaCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaa                                                                              <  1:141427/80‑1 (MQ=255)

                                                                                                                    aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAc              <  2:1339898/139‑1 (MQ=255)

                                                                                                                    aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAc              >  1:1339898/1‑139 (MQ=255)

                                                                                                                      tGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                           <  2:652857/108‑1 (MQ=255)

                                                                                                                      tGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                           >  1:652857/1‑108 (MQ=255)

                                                                                                                                gAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATTAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                   <  2:991508/122‑1 (MQ=255)

                                                                                                                                gAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTa  >  1:1099981/1‑139 (MQ=255)

                                                                                                                                gAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTGACCAGt                   >  1:991508/1‑122 (MQ=255)

GCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTA  >  NC_000913/3927254‑3927520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A