Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,756	C→T	112 (TAG→TAA)	mazF ←	mRNA interferase toxin, antitoxin is MazE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,756	0	C	T	100.0%	123.8 / NA	39	112 (TAG→TAA)	mazF	mRNA interferase toxin, antitoxin is MazE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/18); total (21/18)

GGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCA  >  NC_000913/2910621‑2910885

gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTAc                                                                                                                                >  2:1240345/1‑139 (MQ=255)

    aGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCaa                                                                                                                            >  1:678231/1‑139 (MQ=255)

     gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAAt                                                                                                                           <  2:678231/139‑1 (MQ=255)

           ttCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATc                                                                                                                          >  2:1334144/1‑134 (MQ=255)

           ttCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATc                                                                                                                          <  1:1334144/134‑1 (MQ=255)

                gATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTaa                                                                                                                >  1:306829/1‑139 (MQ=255)

                  tCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAAtt                                                                                                              <  1:1478072/139‑1 (MQ=255)

                        aGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCt                                                                                                        <  2:306829/139‑1 (MQ=255)

                           cgcTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                     <  1:805910/139‑1 (MQ=255)

                              tGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                  <  1:1331386/139‑1 (MQ=255)

                                 ataataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGTGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                               <  1:1240345/139‑1 (MQ=255)

                                          aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAAttt                                                                                                             >  1:1143264/1‑116 (MQ=255)

                                          aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAAttt                                                                                                             <  2:1143264/116‑1 (MQ=255)

                                          aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                      >  1:274165/1‑139 (MQ=255)

                                            cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCctc                                                                                    <  1:516977/139‑1 (MQ=255)

                                               aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTgg                                                                                 >  2:1117521/1‑139 (MQ=255)

                                                  gTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATATTGGCTTTAATGAGTTGTAATTACTCTGGGGc                                                                              >  2:464398/1‑139 (MQ=255)

                                                                        tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCg                                                             >  1:618322/1‑134 (MQ=255)

                                                                        tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCg                                                             <  2:618322/134‑1 (MQ=255)

                                                                              aaaTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCtt                                                  <  2:274165/139‑1 (MQ=255)

                                                                                  tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGccc                                              >  2:216062/1‑139 (MQ=255)

                                                                                                 tGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTaa                                                                                                    >  1:602973/1‑70 (MQ=255)

                                                                                                 tGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTaa                                                                                                    <  2:602973/70‑1 (MQ=255)

                                                                                                      aCCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAcc                          >  1:1500161/1‑139 (MQ=255)

                                                                                                          gtgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGAt                      <  2:1500161/139‑1 (MQ=255)

                                                                                                           tgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATc                                                                                                                          >  1:245101/1‑38 (MQ=255)

                                                                                                           tgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATc                                                                                                                          <  2:245101/38‑1 (MQ=38)

                                                                                                           tgACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                     >  1:1438865/1‑139 (MQ=255)

                                                                                                                  aaTAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAAc              >  2:1158432/1‑139 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                     >  1:1171260/1‑42 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                     <  2:1171260/42‑1 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGa                                                                                                 >  2:1118229/1‑46 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGa                                                                                                 <  1:1118229/46‑1 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt    >  2:365862/1‑139 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt    >  2:456800/1‑139 (MQ=255)

                                                                                                                            aGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCAt    >  2:1160645/1‑139 (MQ=255)

                                                                                                                              ttAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                     <  1:33053/104‑1 (MQ=255)

                                                                                                                              ttAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                     >  2:33053/1‑104 (MQ=255)

                                                                                                                              ttAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCa  <  1:1160645/139‑1 (MQ=255)

GGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCA  >  NC_000913/2910621‑2910885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A