Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,201,206 G→A intergenic (‑241/‑1) ygiF ← / → ygiM inorganic triphosphatase/SH3 domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,201,2060GA100.0% 226.3 / NA 69intergenic (‑241/‑1)ygiF/ygiMinorganic triphosphatase/SH3 domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/35);  total (34/35)

GGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTGATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATC  >  NC_000913/3201103‑3201339
                                                                                                       |                                                                                                                                     
ggCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  2:1447226/139‑1 (MQ=255)
           acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  <  2:646725/98‑1 (MQ=255)
           acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  >  1:646725/1‑98 (MQ=255)
           acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAgcg                                                                                         >  1:1037293/1‑139 (MQ=255)
           acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAgcg                                                                                         >  2:328240/1‑139 (MQ=255)
                 agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   <  2:114474/139‑1 (MQ=255)
                 agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   >  1:887322/1‑139 (MQ=255)
                     ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGa                                                                                                                    >  2:409791/1‑102 (MQ=255)
                     ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGa                                                                                                                    <  1:409791/102‑1 (MQ=255)
                     ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  1:1301175/118‑1 (MQ=255)
                     ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    >  2:1301175/1‑118 (MQ=255)
                           ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               >  2:565459/1‑101 (MQ=255)
                           ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               <  1:565459/101‑1 (MQ=255)
                           ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:662844/139‑1 (MQ=255)
                           ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:328240/139‑1 (MQ=255)
                                gTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   >  2:1495950/1‑92 (MQ=255)
                                gTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   <  1:1495950/92‑1 (MQ=255)
                                     aTGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTgaagaa                                                               <  2:1037293/139‑1 (MQ=255)
                                        cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTa                                                                                                      <  2:633612/97‑1 (MQ=255)
                                        cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTa                                                                                                      >  1:633612/1‑97 (MQ=255)
                                        cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:97846/1‑124 (MQ=255)
                                        cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:97846/124‑1 (MQ=255)
                                         tttGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAAcgc                                                           >  1:453315/1‑139 (MQ=255)
                                         tttGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAAcgc                                                           >  2:304290/1‑139 (MQ=255)
                                             cGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         >  2:1344206/1‑121 (MQ=255)
                                             cGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:1344206/121‑1 (MQ=255)
                                                  cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAc                                                                                                          <  1:177985/83‑1 (MQ=255)
                                                  cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAc                                                                                                          >  2:177985/1‑83 (MQ=255)
                                                  cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGTCGTCTca                                                                           <  1:299761/114‑1 (MQ=255)
                                                  cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGTCGTCTca                                                                           >  2:299761/1‑114 (MQ=255)
                                                       cTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcg                                               >  1:1047141/1‑137 (MQ=255)
                                                       cTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcg                                               <  2:1047141/137‑1 (MQ=255)
                                                              cACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGa                                      <  2:453315/139‑1 (MQ=255)
                                                               actactATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCt                                                        >  1:623005/1‑120 (MQ=255)
                                                               actactATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCt                                                        <  2:623005/120‑1 (MQ=255)
                                                                  actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:1261694/98‑1 (MQ=255)
                                                                  actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:1261694/1‑98 (MQ=255)
                                                                           ccACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCg                         >  2:606482/1‑139 (MQ=255)
                                                                            cACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGc                        <  2:810464/139‑1 (MQ=255)
                                                                              cTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:1086269/88‑1 (MQ=255)
                                                                              cTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         >  2:1086269/1‑88 (MQ=255)
                                                                                ttCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  1:1251366/1‑84 (MQ=255)
                                                                                ttCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  2:1251366/84‑1 (MQ=255)
                                                                                 tCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  1:1417132/58‑1 (MQ=255)
                                                                                 tCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    >  2:1417132/1‑58 (MQ=255)
                                                                                   cATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      <  2:635127/134‑1 (MQ=255)
                                                                                   cATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  1:635127/1‑134 (MQ=255)
                                                                                      gACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCgg              <  2:887322/139‑1 (MQ=255)
                                                                                         aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  1:12166/1‑75 (MQ=255)
                                                                                         aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  2:12166/75‑1 (MQ=255)
                                                                                         aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTc                           <  1:837791/123‑1 (MQ=255)
                                                                                         aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTc                           >  2:837791/1‑123 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  2:1387867/48‑1 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    >  1:1387867/1‑48 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   <  1:1248991/65‑1 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   >  2:1248991/1‑65 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  2:665003/1‑126 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      <  1:665003/126‑1 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGAt         >  1:1438123/1‑139 (MQ=255)
                                                                                           tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGAt         >  1:1005755/1‑139 (MQ=255)
                                                                                                aCAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCa                                                                                                <  1:1257628/47‑1 (MQ=255)
                                                                                                aCAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCa                                                                                                >  2:1257628/1‑47 (MQ=255)
                                                                                                 cAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTAt   <  1:304290/139‑1 (MQ=255)
                                                                                                  aGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATc  >  2:910066/1‑139 (MQ=255)
                                                                                                   gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:155407/1‑65 (MQ=255)
                                                                                                   gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:155407/65‑1 (MQ=255)
                                                                                                   gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  2:520817/1‑118 (MQ=255)
                                                                                                   gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      <  1:520817/118‑1 (MQ=255)
                                                                                                    ccTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACt                                                                                    <  2:128253/55‑1 (MQ=255)
                                                                                                       |                                                                                                                                     
GGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTGATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATC  >  NC_000913/3201103‑3201339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A