Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,141	C→T	123 (TAG→TAA)	yhdN ←	DUF1992 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,141	0	C	T	100.0%	162.8 / NA	51	123 (TAG→TAA)	yhdN	DUF1992 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/21); total (30/21)

GCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCAT  >  NC_000913/3439017‑3439274

gCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAt                                                                                                                         >  1:1185707/1‑139 (MQ=255)

  gAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAttt                                                                                                                       >  2:554639/1‑139 (MQ=255)

     aCCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGt                                                                                                                    >  1:432388/1‑139 (MQ=255)

     aCCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGt                                                                                                                    >  2:1088472/1‑139 (MQ=255)

         aCCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCcaa                                                                                                                >  1:1184888/1‑139 (MQ=255)

          ccTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCcaac                                                                                                               <  1:1088472/139‑1 (MQ=255)

                  aCGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTc                                                                                                       <  2:432388/139‑1 (MQ=255)

                          cATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTGGTCAGCATAAt                                                                                               >  1:986582/1‑139 (MQ=255)

                                ccATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgccgc                                                                                         >  1:1108428/1‑139 (MQ=255)

                                  atcatcTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGt                                                                                       >  2:361790/1‑139 (MQ=255)

                                    catcTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaa                                                                                     >  2:1417637/1‑139 (MQ=255)

                                         gctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                <  2:1108428/139‑1 (MQ=255)

                                           tgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCg                                                                              >  1:324883/1‑139 (MQ=255)

                                                ttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                         >  2:314434/1‑139 (MQ=255)

                                                       taaCGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCa                                                                  >  2:1135334/1‑139 (MQ=255)

                                                             aTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGt                                                            <  1:361790/139‑1 (MQ=255)

                                                                 tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCa                                                        >  1:1136285/1‑139 (MQ=255)

                                                                      ggTGTTACTTCCGCCATTTCTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCTACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCTCAGCTTc                                                   <  2:986582/139‑1 (MQ=255)

                                                                          ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                        >  1:1350751/1‑66 (MQ=255)

                                                                          ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                        <  2:1350751/66‑1 (MQ=255)

                                                                          ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                <  1:572539/106‑1 (MQ=255)

                                                                          ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                >  2:572539/1‑106 (MQ=255)

                                                                          ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                               <  2:419098/139‑1 (MQ=255)

                                                                          ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                               <  2:1136285/139‑1 (MQ=255)

                                                                           tACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTc                                              <  1:1417637/139‑1 (MQ=255)

                                                                            aCTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTcc                                             >  1:81345/1‑139 (MQ=255)

                                                                                  gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTCGTCGCAGCTTCAATTCCAGTAAt                                       >  2:1257625/1‑139 (MQ=255)

                                                                                      tttttGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACa                                   >  1:1127413/1‑139 (MQ=255)

                                                                                       ttttGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACaa                                  >  2:698732/1‑139 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATc                                                                    <  1:1501391/96‑1 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATc                                                                    >  2:1501391/1‑96 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                         <  1:698732/139‑1 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                         >  2:195802/1‑139 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                         >  2:1488123/1‑139 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                         <  2:1185707/139‑1 (MQ=255)

                                                                                                cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                         <  2:1184888/139‑1 (MQ=255)

                                                                                                  cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                       <  1:195802/139‑1 (MQ=255)

                                                                                                  cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                       <  2:1127413/139‑1 (MQ=255)

                                                                                                   aCCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaaaa                                                                                   <  2:373882/78‑1 (MQ=255)

                                                                                                   aCCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaaaa                                                                                   >  1:373882/1‑78 (MQ=255)

                                                                                                        tGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCtt                 >  1:247678/1‑139 (MQ=255)

                                                                                                          cGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGa               >  2:78573/1‑139 (MQ=255)

                                                                                                                   catacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                       >  2:373539/1‑122 (MQ=255)

                                                                                                                   catacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                       <  1:373539/122‑1 (MQ=255)

                                                                                                                       cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                           >  2:725249/1‑50 (MQ=255)

                                                                                                                       cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                           <  1:725249/50‑1 (MQ=255)

                                                                                                                       cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTa                                                                                      >  1:660895/1‑55 (MQ=255)

                                                                                                                       cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTa                                                                                      <  2:660895/55‑1 (MQ=255)

                                                                                                                       cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcat  >  2:149740/1‑139 (MQ=255)

                                                                                                                        tCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCg                               <  2:215293/109‑1 (MQ=255)

                                                                                                                        tCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCg                               >  1:215293/1‑109 (MQ=255)

GCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCAT  >  NC_000913/3439017‑3439274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A