Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,531,180	G→A	A420A (GCC→GCT)	yhgE ←	DUF4153 family putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,531,180	0	G	A	100.0%	139.8 / NA	46	A420A (GCC→GCT)	yhgE	DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/24); total (22/24)

ATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCGGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATA  >  NC_000913/3531070‑3531315

aTTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             <  1:697638/139‑1 (MQ=255)

aTTAACACATTATCGGCTAATACTTTTTCCGATACCTGTGGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCTAGCgcag                                                                                                             <  1:512338/139‑1 (MQ=255)

       caTTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTcc                                                                                                      >  1:302664/1‑139 (MQ=255)

           aTCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             >  1:837056/1‑128 (MQ=255)

           aTCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             <  2:837056/128‑1 (MQ=255)

                  aaTACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCCTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGc                                                                                           >  1:307581/1‑139 (MQ=255)

                        ttttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAc                                                                                              >  2:262681/1‑130 (MQ=255)

                        ttttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAc                                                                                              <  1:262681/130‑1 (MQ=255)

                         tttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTc                                                                                    >  1:372217/1‑139 (MQ=255)

                          ttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCg                                                                                   <  1:728433/139‑1 (MQ=255)

                                    tGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCCTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGa                                                                         <  2:307581/139‑1 (MQ=255)

                                       tGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAg                                                                      >  2:702429/1‑139 (MQ=255)

                                                 gTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCg                                                                                            <  2:774657/107‑1 (MQ=255)

                                                 gTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCg                                                                                            >  1:774657/1‑107 (MQ=255)

                                                   tGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagc                                                                                                            >  2:757937/1‑89 (MQ=255)

                                                   tGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagc                                                                                                            <  1:757937/89‑1 (MQ=255)

                                                   tGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTg                                                          >  2:804465/1‑139 (MQ=255)

                                                      tctcCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             <  1:153141/85‑1 (MQ=255)

                                                      tctcCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                             >  2:153141/1‑85 (MQ=255)

                                                      tctcCATCTAACGCCATCAGCAGATCCCGCGAGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACATGGTATGACg                                                       >  2:775077/1‑139 (MQ=255)

                                                          cATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCTATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTc                                                            >  2:395800/1‑130 (MQ=255)

                                                          cATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCTATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTc                                                            <  1:395800/130‑1 (MQ=255)

                                                                 cGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCg                                            >  1:652772/1‑139 (MQ=255)

                                                                 cGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCg                                            >  2:243154/1‑139 (MQ=255)

                                                                    cATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagca                                                                                                           <  1:269588/73‑1 (MQ=255)

                                                                    cATCAGCAGATCACGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcagca                                                                                                           >  2:269588/1‑73 (MQ=255)

                                                                     aTCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGctct                                        <  1:267959/139‑1 (MQ=255)

                                                                      tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGc                                                                                <  2:405263/98‑1 (MQ=255)

                                                                      tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGc                                                                                >  1:405263/1‑98 (MQ=255)

                                                                            gATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAAcg                                 <  1:211295/139‑1 (MQ=255)

                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTc                                                                                                                     <  2:373488/54‑1 (MQ=255)

                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTc                                                                                                                     >  1:373488/1‑54 (MQ=255)

                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 >  1:517481/1‑90 (MQ=255)

                                                                             aTCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 <  2:517481/90‑1 (MQ=255)

                                                                              tCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATCGCGACCGCTCTGCTCGAGCATGTAGATAg                                                                      >  2:352172/1‑100 (MQ=255)

                                                                              tCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAg                                                                      <  1:352172/100‑1 (MQ=255)

                                                                                   cgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACt                                                                                                                      >  2:82668/1‑47 (MQ=255)

                                                                                   cgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACt                                                                                                                      <  1:82668/47‑1 (MQ=255)

                                                                                    gcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCatat                         >  1:400661/1‑139 (MQ=255)

                                                                                           tCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGtt                  <  1:804465/139‑1 (MQ=255)

                                                                                             gggTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCa                <  1:94036/139‑1 (MQ=255)

                                                                                                 cTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCt            <  2:372217/139‑1 (MQ=255)

                                                                                                 cTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCt            <  2:87598/139‑1 (MQ=255)

                                                                                                    tCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 <  2:50424/67‑1 (MQ=255)

                                                                                                    tCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAg                                                                                 >  1:50424/1‑67 (MQ=255)

                                                                                                           cccAGCATCGCGTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCGTCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATa  <  1:547698/139‑1 (MQ=255)

ATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCGGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATA  >  NC_000913/3531070‑3531315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A