Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,917	G→A	G112S (GGC→AGC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,917	0	G	A	100.0%	149.6 / NA	46	G112S (GGC→AGC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/24); total (22/24)

AAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAT  >  NC_000913/2935783‑2936031

aaGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCAc                                                                                                                >  1:135418/1‑139 (MQ=255)

         ctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGccc                                                                                                       <  2:135418/139‑1 (MQ=255)

         ctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCGTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGTCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGccc                                                                                                       <  2:489889/139‑1 (MQ=255)

            gCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCgg                                                                                                    <  1:882958/139‑1 (MQ=255)

             cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGgc                                                                                                   <  1:1010970/139‑1 (MQ=255)

              gaagaaAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGgcg                                                                                                  <  1:636109/139‑1 (MQ=255)

                                 aGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCgg                                                                                                    >  2:455788/1‑118 (MQ=255)

                                 aGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCgg                                                                                                    <  1:455788/118‑1 (MQ=255)

                                         ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAg                                                                       >  2:71071/1‑139 (MQ=255)

                                            cTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTc                                                                    <  1:71071/139‑1 (MQ=255)

                                               aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcaca                                                                 >  1:346023/1‑139 (MQ=255)

                                               aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTc                                                                    >  2:768285/1‑136 (MQ=255)

                                               aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTc                                                                    <  1:768285/136‑1 (MQ=255)

                                                      cGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaa                                                          <  2:606951/139‑1 (MQ=255)

                                                         tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGgc                                                                                                   >  1:740403/1‑95 (MQ=255)

                                                         tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGgc                                                                                                   <  2:740403/95‑1 (MQ=255)

                                                           aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGccc                                                                                                       >  2:1078293/1‑89 (MQ=255)

                                                           aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGccc                                                                                                       <  1:1078293/89‑1 (MQ=255)

                                                           aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTAc                                                                                          <  2:928762/102‑1 (MQ=255)

                                                           aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTAc                                                                                          >  1:928762/1‑102 (MQ=255)

                                                           aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCa                                                     >  1:2705/1‑139 (MQ=255)

                                                           aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCa                                                     >  1:962251/1‑139 (MQ=255)

                                                                ttGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCa                                                <  2:346023/139‑1 (MQ=255)

                                                                   cTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                             <  2:2705/139‑1 (MQ=255)

                                                                       tGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATtct                                         >  2:989578/1‑139 (MQ=255)

                                                                             ggCAGTGAAACCATCGACATGGATCCATCCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCATCATTCTCCAttt                                   >  2:677513/1‑139 (MQ=255)

                                                                                  tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                              >  1:920540/1‑139 (MQ=255)

                                                                                      aCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                          >  1:206962/1‑139 (MQ=255)

                                                                                        cATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                             <  2:265978/118‑1 (MQ=255)

                                                                                        cATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                             >  1:265978/1‑118 (MQ=255)

                                                                                         aTCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                            <  1:109540/134‑1 (MQ=255)

                                                                                         aTCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                            >  2:109540/1‑134 (MQ=255)

                                                                                           cGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                     >  2:601279/1‑139 (MQ=255)

                                                                                           cGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                     <  2:206962/139‑1 (MQ=255)

                                                                                               aTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCCGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                 >  2:825946/1‑139 (MQ=255)

                                                                                               aTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                 <  1:989578/139‑1 (MQ=255)

                                                                                                  gATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGa                                                           <  1:701780/94‑1 (MQ=255)

                                                                                                  gATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGa                                                           >  2:701780/1‑94 (MQ=255)

                                                                                                   aTCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGc             <  2:842523/139‑1 (MQ=255)

                                                                                                       aaCCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                          <  2:456129/122‑1 (MQ=255)

                                                                                                       aaCCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                          >  1:456129/1‑122 (MQ=255)

                                                                                                         cccgcccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCTCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                       >  2:698630/1‑91 (MQ=255)

                                                                                                         cccgcccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                       <  1:698630/91‑1 (MQ=255)

                                                                                                              ccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt  <  2:962251/139‑1 (MQ=255)

                                                                                                                              gCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                          >  1:785363/1‑99 (MQ=255)

                                                                                                                              gCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                          <  2:785363/99‑1 (MQ=255)

AAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAT  >  NC_000913/2935783‑2936031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A