Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A1 F1 I2 R1 | 765 | 46.1 | 2269152 | 93.8% | 2128464 | 105.7 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,001,304 | (G)7→9 | coding (960/2259 nt) | xdhA → | xanthine dehydrogenase, molybdenum binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,001,297 | 1 | . | G | 100.0% | 136.5 / NA | 38 | A318G (GCG→GGG) | xdhA | xanthine dehydrogenase, molybdenum binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (21/17); total (21/17) | |||||||||||
| * | NC_000913 | 3,001,297 | 2 | . | G | 92.3% | 128.8 / 3.6 | 39 | coding (953/2259 nt) | xdhA | xanthine dehydrogenase, molybdenum binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (20/16); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
CCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTT > NC_000913/3001165‑3001427 || cccGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑gggggg < 1:1004356/139‑1 (MQ=255) tttACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTa > 2:855579/1‑139 (MQ=255) gACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATcc > 1:238725/1‑139 (MQ=255) ggCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt > 1:342332/1‑139 (MQ=255) gCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTAc > 1:153945/1‑139 (MQ=255) gTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGc > 1:551299/1‑139 (MQ=255) tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc < 2:320913/133‑1 (MQ=255) tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc > 1:320913/1‑133 (MQ=255) cgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGtt > 1:1057354/1‑139 (MQ=255) gAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg < 2:272348/139‑1 (MQ=255) ttATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑gggggg < 2:551866/72‑1 (MQ=255) ttATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑gggggg > 1:551866/1‑72 (MQ=255) ataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAc < 2:1057354/139‑1 (MQ=255) tGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTCCTGTTCAAAGACCTCCTATCCCAACCTcccc > 1:931024/1‑139 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt < 1:293407/92‑1 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt > 2:293407/1‑92 (MQ=255) tGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCgg < 1:552143/139‑1 (MQ=255) gTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg < 2:796629/108‑1 (MQ=255) gTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg > 1:796629/1‑108 (MQ=255) acacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg < 2:153945/139‑1 (MQ=255) cacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg < 1:43699/133‑1 (MQ=255) cacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg > 2:43699/1‑133 (MQ=255) cGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTg > 1:407874/1‑139 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGt > 2:605581/1‑139 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGGATAAAGTCGCt > 1:305083/1‑57 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGGATAAAGTCGCt < 2:305083/57‑1 (MQ=255) ctcACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTtgtg > 1:112994/1‑65 (MQ=255) ctcACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCt > 1:898862/1‑89 (MQ=255) ctcACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCt < 2:898862/89‑1 (MQ=255) ctaaCGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTtgtg < 2:112994/62‑1 (MQ=255) cACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTAc < 2:704896/74‑1 (MQ=255) cACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCt < 2:333247/87‑1 (MQ=255) cACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCt > 1:333247/1‑87 (MQ=255) cACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACACTTAc > 1:704896/1‑74 (MQ=255) cGGGCCCTCCATCGCTTCTGCGGGGGGGGGAATAAATTCCCTTACCTTTATCCTCGTTGTTCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg < 2:931024/139‑1 (MQ=255) cACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTtgtg > 2:618511/1‑57 (MQ=255) cACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTtgtg < 1:618511/57‑1 (MQ=255) cTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCt < 2:79627/36‑1 (MQ=255) cTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCt > 1:79627/1‑36 (MQ=255) cTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt < 2:423255/51‑1 (MQ=255) cTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt > 1:423255/1‑51 (MQ=255) gCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt < 1:1039186/139‑1 (MQ=255) || CCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTT > NC_000913/3001165‑3001427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |