Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,491,137 |
T→C |
E109E (GAA→GAG) |
fic ← |
stationary phase‑induced protein, putative toxin |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,491,137 | 0 | T | C | 100.0%
| 157.5
/ NA
| 44 | E109E (GAA→GAG) | fic | stationary phase‑induced protein, putative toxin |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/24); total (20/24) |
GAAGATCCGCTGTGCCAGACCACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCTTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAGTTGCCCTGCCCAGTCGAAAATATCCTGATA > NC_000913/3491020‑3491265
|
gaagaTCCGCTGTGCCAGACCACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATc > 1:923876/1‑139 (MQ=255)
gtgCCAGACCACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGcc < 2:103197/139‑1 (MQ=255)
ccAGACCACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATc < 2:1013459/118‑1 (MQ=255)
ccAGACCACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATc > 1:1013459/1‑118 (MQ=255)
ccACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACcctct < 1:292999/102‑1 (MQ=255)
ccACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACcctct > 2:292999/1‑102 (MQ=255)
cTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCa > 1:459455/1‑118 (MQ=255)
cTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCa < 2:459455/118‑1 (MQ=255)
cccACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATc > 2:168409/1‑107 (MQ=255)
cccACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATc < 1:168409/107‑1 (MQ=255)
aCCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAg > 2:991320/1‑139 (MQ=255)
ccGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCa > 1:74379/1‑110 (MQ=255)
ccGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCa < 2:74379/110‑1 (MQ=255)
ccGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCa < 2:312903/139‑1 (MQ=255)
tAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCAt < 1:217142/74‑1 (MQ=255)
tAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCAt > 2:217142/1‑74 (MQ=255)
tAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAatat < 1:814998/139‑1 (MQ=255)
tAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAatat > 2:995864/1‑139 (MQ=255)
aTGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCa > 2:942362/1‑111 (MQ=255)
aTGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCa < 1:942362/111‑1 (MQ=255)
tGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACcctctt < 2:954838/55‑1 (MQ=255)
tGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACcctctt > 1:954838/1‑55 (MQ=255)
cgcCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCAt < 2:827287/69‑1 (MQ=255)
cgcCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCAt > 1:827287/1‑69 (MQ=255)
ccAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCct > 2:283462/1‑81 (MQ=255)
ccAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCct < 1:283462/81‑1 (MQ=255)
aGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGcc > 1:760594/1‑77 (MQ=255)
aGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGcc < 2:760594/77‑1 (MQ=255)
gCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACcctct > 2:418964/1‑47 (MQ=255)
gCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACcctct < 1:418964/47‑1 (MQ=255)
gCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACg > 2:627600/1‑139 (MQ=255)
gCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGAGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACg > 2:21591/1‑139 (MQ=255)
ccGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGTCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTCATAAATATCAACTTCACGc < 2:923876/139‑1 (MQ=255)
gCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAg < 1:995864/139‑1 (MQ=255)
gCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAg < 1:643236/139‑1 (MQ=255)
gCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAg < 1:564406/139‑1 (MQ=255)
cTTCGCTTTCTCCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGAGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAGTtgccctgcc < 1:21591/139‑1 (MQ=255)
ctcCAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAGTTGCCCTGCCCAGTCGaaa < 1:627600/139‑1 (MQ=255)
cAGGCCAACCAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAGTTGCCCTGCCCAGTCGAAAata < 1:492601/139‑1 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGc > 1:65913/1‑42 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGc < 2:65913/42‑1 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCct > 2:427086/1‑45 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCct < 1:427086/45‑1 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGat > 2:191189/1‑55 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGat < 1:191189/55‑1 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGa < 2:371336/89‑1 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGa > 1:371336/1‑89 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACg > 2:1108165/1‑106 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACg < 1:1108165/106‑1 (MQ=255)
ccAGATAACCCTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAGTTGCCCTGCCCAGTCGAAAATATCCTGATa > 1:430824/1‑139 (MQ=255)
|
GAAGATCCGCTGTGCCAGACCACTTCCCACCCGGAAGGGATGCAGCACGTTGATTTCACAATAGTAATGCGCCAGCCGCTCGACGAACTTCGCTTTCTCCAGGCCAACCAGATAACCTTCTTCCTCCAGATCCTGCATCAGGGCATTGCCCTCTTTTTCGATATAAGCAAAGTGGCAGAACGGCGTATCACCCTGATAAATATCAACTTCACGCAGTTGCCCTGCCCAGTCGAAAATATCCTGATA > NC_000913/3491020‑3491265
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
