Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 760 67.6 3323148 92.7% 3080558 109.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,269,012 A→G S200G (AGT→GGT)  yhaC → pentapetide repeats‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,0120AG100.0% 254.0 / NA 70S200G (AGT→GGT) yhaCpentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/30);  total (40/30)

TCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG  >  NC_000913/3268879‑3269141
                                                                                                                                     |                                                                                                                                 
tctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCa                                                                                                                              >  2:1412666/1‑139 (MQ=255)
  tGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAac                                                                                                                            >  1:1330542/1‑139 (MQ=255)
  tGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAac                                                                                                                            >  2:612164/1‑139 (MQ=255)
         aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAAc                                                                                                                     >  1:908956/1‑139 (MQ=255)
             aaTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCca                                                                                                                 <  2:1429454/139‑1 (MQ=255)
                              ttatGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGattat                                                                                                >  2:249290/1‑139 (MQ=255)
                               tatGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGattatt                                                                                               >  2:91263/1‑139 (MQ=255)
                                    tttCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTa                                                                                          <  1:249290/139‑1 (MQ=255)
                                       cacaGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTaaaa                                                                                       >  1:1402829/1‑139 (MQ=255)
                                        acaGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  1:215357/1‑114 (MQ=255)
                                        acaGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  2:215357/114‑1 (MQ=255)
                                        acaGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTaaaaa                                                                                      >  2:1487708/1‑139 (MQ=255)
                                              aaaCATATGGCTAATACTATTTTAAATCCTTTTATATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                <  1:1487708/139‑1 (MQ=255)
                                               aaCATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGcc                                                                               >  1:971511/1‑139 (MQ=255)
                                                      ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                         <  2:1225841/106‑1 (MQ=255)
                                                      ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                         >  1:1225841/1‑106 (MQ=255)
                                                      ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                        >  2:465458/1‑139 (MQ=255)
                                                        cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                         >  1:33536/1‑104 (MQ=255)
                                                        cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                         <  2:33536/104‑1 (MQ=255)
                                                        cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                      >  2:57509/1‑139 (MQ=255)
                                                        cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                      <  1:1412666/139‑1 (MQ=255)
                                                         tAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                     <  1:619997/139‑1 (MQ=255)
                                                           aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  1:1466340/1‑95 (MQ=255)
                                                           aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  2:1466340/95‑1 (MQ=255)
                                                            tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGc                                                                  >  1:637004/1‑139 (MQ=255)
                                                            tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAAAGCCTGTTTTAATAAGc                                                                  >  1:1031482/1‑139 (MQ=255)
                                                             aCTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                 <  2:1402829/139‑1 (MQ=255)
                                                                 ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaa                                                                       <  2:1071177/129‑1 (MQ=255)
                                                                 ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaa                                                                       >  1:1071177/1‑129 (MQ=255)
                                                                 ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAg                                                             >  1:557828/1‑139 (MQ=255)
                                                                 ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAg                                                             >  1:136507/1‑139 (MQ=255)
                                                                     aaaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGat                                                         <  2:557828/139‑1 (MQ=255)
                                                                       aTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:995337/1‑83 (MQ=255)
                                                                       aTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:995337/83‑1 (MQ=255)
                                                                           ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:1436664/1‑79 (MQ=255)
                                                                           ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:1436664/79‑1 (MQ=255)
                                                                           ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcac                                                                                                                >  2:999923/1‑78 (MQ=255)
                                                                           ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcac                                                                                                                <  1:999923/78‑1 (MQ=255)
                                                                                  ttGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTgaag                                            <  2:1330542/139‑1 (MQ=255)
                                                                                     aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                         <  1:540127/75‑1 (MQ=255)
                                                                                     aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                         >  2:540127/1‑75 (MQ=255)
                                                                                     aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTg                                                                                                       >  1:1388378/1‑77 (MQ=255)
                                                                                     aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTg                                                                                                       <  2:1388378/77‑1 (MQ=255)
                                                                                          aacaaATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTg                                                                             >  1:714933/1‑98 (MQ=255)
                                                                                          aacaaATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTg                                                                             <  2:714933/98‑1 (MQ=255)
                                                                                               aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTa                                                                                          <  1:629576/80‑1 (MQ=255)
                                                                                               aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTa                                                                                          >  2:629576/1‑80 (MQ=255)
                                                                                                        aTGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                <  2:479521/81‑1 (MQ=255)
                                                                                                        aTGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                >  1:479521/1‑81 (MQ=255)
                                                                                                          gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgtt                    >  1:794417/1‑139 (MQ=255)
                                                                                                              aCATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                            <  1:191045/95‑1 (MQ=255)
                                                                                                              aCATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                            >  2:191045/1‑95 (MQ=255)
                                                                                                                  gTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa             >  1:1339996/1‑138 (MQ=255)
                                                                                                                  gTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa             <  2:1339996/138‑1 (MQ=255)
                                                                                                                  gTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAAt            >  2:299340/1‑139 (MQ=255)
                                                                                                                  gTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAAt            <  1:612164/139‑1 (MQ=255)
                                                                                                                  gTTTCAGGTTAATTTTGTTGGGTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAAt            <  2:971511/139‑1 (MQ=255)
                                                                                                                   tttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATg           <  1:299340/139‑1 (MQ=255)
                                                                                                                    ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGatat                                                       <  2:1369062/94‑1 (MQ=255)
                                                                                                                    ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGatat                                                       >  1:1369062/1‑94 (MQ=255)
                                                                                                                    ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGa          >  2:584685/1‑139 (MQ=255)
                                                                                                                    ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGa          >  1:273672/1‑139 (MQ=255)
                                                                                                                         gTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                 <  2:1493100/79‑1 (MQ=255)
                                                                                                                         gTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                 >  1:1493100/1‑79 (MQ=255)
                                                                                                                          ttAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTgg    >  1:498925/1‑139 (MQ=255)
                                                                                                                            aaTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgag  >  2:851426/1‑139 (MQ=255)
                                                                                                                             aTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                     >  2:1581176/1‑71 (MQ=255)
                                                                                                                             aTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                     <  1:1581176/71‑1 (MQ=255)
                                                                                                                             aTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaa                                                                       >  1:505290/1‑69 (MQ=255)
                                                                                                                             aTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaa                                                                       <  2:505290/69‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
TCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG  >  NC_000913/3268879‑3269141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A