Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,859,538	C→T	L816L (CTG→TTG)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,859,538	0	C	T	100.0%	138.9 / NA	43	L816L (CTG→TTG)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/22); total (21/22)

ACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAG  >  NC_000913/2859404‑2859670

aCGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTc                                                                                                                                  <  1:1073947/139‑1 (MQ=255)

  ggCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAg                                                                                                                                <  2:763793/139‑1 (MQ=255)

     cTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTAc                                                                                                                             <  2:539038/139‑1 (MQ=255)

       ggCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCa                                                                                                                           >  2:376090/1‑139 (MQ=255)

          ggTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCACCGCTACGCAAGTGGATGGTACGGAAATGTCTTTGTTGTCAGTACCagaa                                                                                                                        <  1:1215801/139‑1 (MQ=255)

             tGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAgaagaa                                                                                                                     >  2:1055525/1‑139 (MQ=255)

                    ctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGc                                                                                                              >  1:647657/1‑139 (MQ=255)

                       gCAGGCGTGCCAAAAGATGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTg                                                                                                           >  1:821033/1‑139 (MQ=255)

                                  aaaaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGct                                                                                                <  2:315300/139‑1 (MQ=255)

                                         gTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                                                         >  1:395766/1‑139 (MQ=255)

                                          ttattaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaaa                                                                                        >  1:721764/1‑139 (MQ=255)

                                                  cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCagaaga                                                                                                                      >  1:448593/1‑101 (MQ=255)

                                                  cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCagaaga                                                                                                                      <  2:448593/101‑1 (MQ=255)

                                                  cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                <  1:508406/139‑1 (MQ=255)

                                                      cACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                                                            >  2:471905/1‑139 (MQ=255)

                                                           cAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcac                                                                       <  2:395766/139‑1 (MQ=255)

                                                            aaaaGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcact                                                                      <  1:376090/139‑1 (MQ=255)

                                                                   gCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcg                                                               >  2:723795/1‑139 (MQ=255)

                                                                   gCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcg                                                               >  1:629431/1‑139 (MQ=255)

                                                                     gTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCGCTCACCCcgcg                                                             >  1:857390/1‑139 (MQ=255)

                                                                     gTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcgcg                                                             >  1:335519/1‑139 (MQ=255)

                                                                          cTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAgg                                                        >  1:898706/1‑139 (MQ=255)

                                                                            ggAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcg                                                      <  1:893993/139‑1 (MQ=255)

                                                                                    tttCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTAcc                                                                                                                            >  2:804050/1‑61 (MQ=255)

                                                                                    tttCGCCGAACGCCGCCGCTACGCAAGTGGATGGTAAGCAAATGTCTTTGTTGACAGTAcc                                                                                                                            <  1:804050/61‑1 (MQ=255)

                                                                                        gCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAtt                                          >  1:396151/1‑139 (MQ=255)

                                                                                                  gccgcTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGa                                <  1:1055525/139‑1 (MQ=255)

                                                                                                   ccgcTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                               <  2:898706/139‑1 (MQ=255)

                                                                                                        aCGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGcc                          <  1:638740/139‑1 (MQ=255)

                                                                                                           cAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTgg                       <  2:335519/139‑1 (MQ=255)

                                                                                                                      tACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                               <  2:808722/120‑1 (MQ=255)

                                                                                                                      tACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACaa            >  1:875318/1‑139 (MQ=255)

                                                                                                                      tACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACaa            >  1:198163/1‑139 (MQ=255)

                                                                                                                      aaCGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCATGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                               >  1:808722/2‑120 (MQ=255)

                                                                                                                           aaaTGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcg                                                      <  1:737027/92‑1 (MQ=255)

                                                                                                                           aaaTGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcg                                                      >  2:737027/1‑92 (MQ=255)

                                                                                                                             aTGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGa     <  2:914572/139‑1 (MQ=255)

                                                                                                                             aTGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGa     <  2:629431/139‑1 (MQ=255)

                                                                                                                             aTGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGa     <  1:412774/139‑1 (MQ=255)

                                                                                                                               gTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATa   <  1:723795/139‑1 (MQ=255)

                                                                                                                                tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTcc        >  1:246864/1‑133 (MQ=255)

                                                                                                                                tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTcc        <  2:246864/133‑1 (MQ=255)

                                                                                                                                tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAg  >  1:608100/1‑139 (MQ=255)

ACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAG  >  NC_000913/2859404‑2859670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A