Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,173	C→T	212 (TAG→TAA)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,173	0	C	T	100.0%	181.3 / NA	56	212 (TAG→TAA)	argO	arginine transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/26); total (30/26)

TAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCC  >  NC_000913/3068038‑3068303

tAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 <  2:408562/139‑1 (MQ=255)

             tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAg                                                                                                                        >  1:702822/1‑135 (MQ=255)

             tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAg                                                                                                                        <  2:702822/135‑1 (MQ=255)

             tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCtt                                                                                                                    >  2:144565/1‑139 (MQ=255)

               ccgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGAGGTAATTGACTGGATCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAAGTGAAAAAGACTtgt                                                                                                                  >  2:337525/1‑139 (MQ=255)

                 gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  1:694812/139‑1 (MQ=255)

                 gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  1:325269/139‑1 (MQ=255)

                   cAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCAt                                                                                                              >  1:320374/1‑139 (MQ=255)

                      ggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAg                                                                                                           >  2:1215751/1‑139 (MQ=255)

                                       tCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 >  2:464144/1‑100 (MQ=255)

                                       tCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 <  1:464144/100‑1 (MQ=255)

                                               gTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                                      <  2:1264258/103‑1 (MQ=255)

                                               gTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                                      >  1:1264258/1‑103 (MQ=255)

                                                tctctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAg                                                                                 >  1:117931/1‑139 (MQ=255)

                                                  tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGG‑GGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              <  2:1014846/139‑1 (MQ=255)

                                                     ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAAt                                                                            >  2:476332/1‑139 (MQ=255)

                                                      cGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           <  1:667140/139‑1 (MQ=255)

                                                            aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcac                                                                     >  2:935506/1‑139 (MQ=255)

                                                                  cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               <  2:978483/139‑1 (MQ=255)

                                                                  cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               <  1:144565/139‑1 (MQ=255)

                                                                  cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               >  1:978483/1‑139 (MQ=255)

                                                                   ttGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                   >  1:253384/1‑118 (MQ=255)

                                                                   ttGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                   <  2:253384/118‑1 (MQ=255)

                                                                       ccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          >  2:511870/1‑139 (MQ=255)

                                                                           aaTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCcaca                                                      <  1:1213727/139‑1 (MQ=255)

                                                                                     ctctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGa                                            >  2:685126/1‑139 (MQ=255)

                                                                                      tctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                                    >  2:350654/1‑114 (MQ=255)

                                                                                      tctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                                    <  1:350654/114‑1 (MQ=255)

                                                                                             gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  1:337525/139‑1 (MQ=255)

                                                                                             gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  1:165134/139‑1 (MQ=255)

                                                                                             gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  2:33129/139‑1 (MQ=255)

                                                                                               aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          >  2:662704/1‑115 (MQ=255)

                                                                                               aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          <  1:662704/115‑1 (MQ=255)

                                                                                               aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                                  >  2:892838/1‑139 (MQ=255)

                                                                                               aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                                  >  2:962711/1‑139 (MQ=255)

                                                                                                aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  2:1053649/1‑139 (MQ=255)

                                                                                                aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  2:1227007/1‑139 (MQ=255)

                                                                                                aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  1:965829/1‑139 (MQ=255)

                                                                                                aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  1:611854/1‑139 (MQ=255)

                                                                                                 gCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgtg                                >  2:995527/1‑139 (MQ=255)

                                                                                                  cTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                    >  2:470810/1‑118 (MQ=255)

                                                                                                  cTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                    <  1:470810/118‑1 (MQ=255)

                                                                                                         ccTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGcc                        <  2:320374/139‑1 (MQ=255)

                                                                                                           tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGt                      >  1:1151001/1‑139 (MQ=255)

                                                                                                           tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGt                      >  1:22185/1‑139 (MQ=255)

                                                                                                            ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              <  2:535419/82‑1 (MQ=255)

                                                                                                            ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              >  1:535419/1‑82 (MQ=255)

                                                                                                            ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                              <  1:665091/98‑1 (MQ=255)

                                                                                                            ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                              >  2:665091/1‑98 (MQ=255)

                                                                                                                       ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  1:888104/37‑1 (MQ=255)

                                                                                                                       ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                >  2:888104/1‑37 (MQ=255)

                                                                                                                       ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           >  2:665057/1‑74 (MQ=255)

                                                                                                                       ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           <  1:665057/74‑1 (MQ=255)

                                                                                                                        tCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGt         <  1:476332/139‑1 (MQ=255)

                                                                                                                               gATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcc  <  1:962711/139‑1 (MQ=255)

                                                                                                                               gATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcc  <  2:22185/139‑1 (MQ=255)

TAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCC  >  NC_000913/3068038‑3068303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A