Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,106,905	T→C	F398L (TTC→CTC)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,106,905	0	T	C	100.0%	215.9 / NA	59	F398L (TTC→CTC)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/35); total (24/35)

TAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGT  >  NC_000913/3106772‑3107039

tAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATg                                                                                                                                   <  2:642838/139‑1 (MQ=255)

 aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATgg                                                                                                                                  <  1:518619/139‑1 (MQ=255)

   cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  2:599840/139‑1 (MQ=255)

   cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  2:4442/139‑1 (MQ=255)

   cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:45586/139‑1 (MQ=255)

   cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACAGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:427330/139‑1 (MQ=255)

        tCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGtt                                                                                                                           >  1:684519/1‑139 (MQ=255)

         cGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTc                                                                                                                          <  1:991926/139‑1 (MQ=255)

               tATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                >  2:920495/1‑127 (MQ=255)

               tATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:920495/127‑1 (MQ=255)

                 tCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATaa                                                                                                                  <  2:684519/139‑1 (MQ=255)

                           cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACTGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAat                                                                                                                      <  1:706275/125‑1 (MQ=255)

                           cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCaa                                                                                                                        <  1:242619/123‑1 (MQ=255)

                           cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCaa                                                                                                                        >  2:242619/1‑123 (MQ=255)

                           cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAat                                                                                                                      >  2:706275/1‑125 (MQ=255)

                                 gAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCg                                                                                                  <  2:298930/139‑1 (MQ=255)

                                            ttgttgATATGTACACCCCAAACGGCATTACCGACTGGCAGACGGTATGGTTGCTTTTTGCTGGTTAATCCGAGGTTCTGGCCTTGGCGCTCATGGCGATGTTCAAATATAAACGCGTTCGTGTCCCGACAGGcacaca                                                                                       <  2:1274691/139‑1 (MQ=255)

                                            ttgttgAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGcacaca                                                                                       <  1:798263/139‑1 (MQ=255)

                                               ttgAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGAc                                                                                    >  2:376786/1‑139 (MQ=255)

                                                  agaTGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGt                                                                                 <  1:1086130/139‑1 (MQ=255)

                                                         cacCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCAc                                                                          >  2:681044/1‑139 (MQ=255)

                                                            ccAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:410422/82‑1 (MQ=255)

                                                            ccAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                >  2:410422/1‑82 (MQ=255)

                                                              aaaaCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCa                                                                                                                         <  2:1067202/87‑1 (MQ=255)

                                                              aaaaCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCa                                                                                                                         >  1:1067202/1‑87 (MQ=255)

                                                               aaaCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTa                                                                    >  2:1269052/1‑139 (MQ=255)

                                                                 aCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACg                                                                  <  2:825955/139‑1 (MQ=255)

                                                                      aTTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTc                                                                                                                          <  2:22743/78‑1 (MQ=255)

                                                                      aTTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTc                                                                                                                          >  1:22743/1‑78 (MQ=255)

                                                                                ggCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGt                                                   <  2:616801/139‑1 (MQ=255)

                                                                                     aCCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                              >  1:1121996/1‑139 (MQ=255)

                                                                                     aCCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                              >  2:1267053/1‑139 (MQ=255)

                                                                                         tATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  2:1040269/53‑1 (MQ=255)

                                                                                         tATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                >  1:1040269/1‑53 (MQ=255)

                                                                                                  ttttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  2:1121996/139‑1 (MQ=255)

                                                                                                  ttttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  1:1269052/139‑1 (MQ=255)

                                                                                                  ttttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  1:1267053/139‑1 (MQ=255)

                                                                                                   tttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGtt                                                                                                                           <  2:74435/48‑1 (MQ=255)

                                                                                                   tttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGtt                                                                                                                           >  1:74435/1‑48 (MQ=255)

                                                                                                       gCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttt                            >  2:502358/1‑139 (MQ=255)

                                                                                                           gTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTAt                        >  1:339059/1‑139 (MQ=255)

                                                                                                              aCTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCt                     <  2:339059/139‑1 (MQ=255)

                                                                                                                tCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTa                   >  2:288404/1‑139 (MQ=255)

                                                                                                                 ccGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTAc                  >  2:60281/1‑139 (MQ=255)

                                                                                                                   gTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACtt                <  1:376786/139‑1 (MQ=255)

                                                                                                                       ttCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAac            >  2:1260633/1‑139 (MQ=255)

                                                                                                                        tCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAaca           <  1:60281/139‑1 (MQ=255)

                                                                                                                          tGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttttt                          >  2:569181/1‑122 (MQ=255)

                                                                                                                          tGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttttt                          <  1:569181/122‑1 (MQ=255)

                                                                                                                           ggCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACAt        <  2:1066250/139‑1 (MQ=255)

                                                                                                                           ggCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACAt        <  1:1260633/139‑1 (MQ=255)

                                                                                                                             ccTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATaa      <  1:288404/139‑1 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 >  2:348044/1‑108 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  1:348044/108‑1 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTTTTACTTCa              >  2:246576/1‑127 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCa              <  1:246576/127‑1 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCa              >  1:700684/1‑127 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGt  >  2:830930/1‑139 (MQ=255)

                                                                                                                                 cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACAAAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCa              <  2:700684/127‑1 (MQ=255)

TAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGT  >  NC_000913/3106772‑3107039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A