Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,742,599 |
Δ1 bp |
intergenic (‑67/‑134) |
yiaJ ← / → yiaK |
transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,742,598 | 0 | C | . | 100.0%
| 95.5
/ NA
| 24 | intergenic (‑66/‑135) | yiaJ/yiaK | transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (12/12); total (12/12) |
GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGTT > NC_000913/3742463‑3742646
|
ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat < 2:747465/139‑4 (MQ=255)
cTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTg < 2:951384/139‑1 (MQ=255)
ttCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGa > 2:254213/1‑139 (MQ=255)
tCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGAt > 1:744260/1‑139 (MQ=255)
cGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGAttt > 2:886281/1‑139 (MQ=255)
gCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGAttttt < 1:254213/139‑1 (MQ=255)
gCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCtt < 1:886281/139‑1 (MQ=255)
tttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGaaa < 1:1000199/139‑1 (MQ=255)
gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGCAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATtcatc > 2:600569/1‑139 (MQ=255)
gcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaa > 1:945531/1‑139 (MQ=255)
tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGTTGGTAGCGCAAAGTGTTCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcct > 2:1050813/1‑137 (MQ=255)
tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATTGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATtc < 2:1018852/130‑1 (MQ=255)
tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATtc > 1:1018852/1‑130 (MQ=255)
cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt < 2:945531/139‑6 (MQ=255)
ttCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGt > 2:39219/1‑113 (MQ=255)
ttCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGt < 1:39219/113‑1 (MQ=255)
ttCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttga < 1:600569/139‑8 (MQ=255)
tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgacaatt > 1:65953/1‑127 (MQ=255)
ttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgacaatta < 2:561679/139‑14 (MQ=255)
ccATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgt < 2:106507/123‑5 (MQ=255)
ccATCACTTCTTTCCCCATTTTGTCGCGTCCTGAGGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgt > 1:106507/1‑119 (MQ=255)
aGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat > 2:166640/1‑47 (MQ=255)
aGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat < 1:166640/50‑4 (MQ=255)
tgCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatc < 2:484376/70‑1 (MQ=255)
tgCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCatcaatc > 1:484376/1‑70 (MQ=255)
ttCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATtcat < 1:984574/57‑1 (MQ=255)
ttCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATtcat > 2:984574/1‑57 (MQ=255)
|
GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGTT > NC_000913/3742463‑3742646
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
